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      • 엄나무 (Kalopanax pictus Nakai) 줄기 추출물이 In vitro 반추위 발효와 메탄저감에 미치는 영향

        김재성,황문석,김용채,윤영만,배귀석,김창현,Kim, Jae Seong,Hwang, Moon Seok,Kim, Yong Chae,Yoon, Young-Man,Bae, Gui Sek,Kim, Chang-Hyun 한국축산환경학회 2015 한국축산시설환경학회지 Vol.21 No.3

        An experiment was conducted to examine the effects of Kalopanax pictus Nakai (Kalopanax) on in vitro rumen fermentation and methane (CH4) reduction. Kalopanax trunk was extracted with 70% ethanol and 70% methanol. Rumen fluid, alfalfa hay and buffer (control: C) supplemented with 0.3% Kalopanx juice (T1), 0.3% ethanol extract (T2) and 0.3% methanol extract (T3) in the total volume of culture medium were incubated at $38^{\circ}C$ for 24h and 48h. Rumen pH was lower in all Kalopanax treatments during all incubations than that in control (p<0.05). Total VFA and total gas production in T2 and T3 was significantly higher than that in C at 48h incubation (p<0.05). Ammonia-N was decreased in all treatments compared with C during the incubation periods (p<0.05). At 24h incubation, $CH_4$ contents were significantly reduced by both alcohol extracts. It is concluded that supplementing Kalopanax extracts can stimulate ruminal fermentation of rumen microorganisms and inhibit methanogenesis.

      • KCI등재

        저선량 $\gamma$선 조사가 토마토의 초기생육과 후속고선량 $\gamma$선 저항성에 미치는 영향

        김재성,김진규,백명화,김동희,Kim, Jae-Sung,Kim, Jin-Kyu,Back, Myung-Hwa,Kim, Dong-Hee 대한방사선방어학회 1999 방사선방어학회지 Vol.24 No.3

        저선량 $\gamma$선 조사한 토마토 2품종, 서광과 하우스모모타로 종자의 발아와 초기생육 및 후속 고선량에 대한 생육효과를 조사하였다. 저선량에 의한 발아와 생육촉진효과는 품종에 따라 달랐다. 발아율의 경우 서광은 모든 저선량 조사구에서 증가되었으나, 하우스모모타로는 오히려 감소되었다. 유묘초장은 저선량 조사에 의해 증가되었으며 두 품종 모두 4 Gy와 8 Gy 조사구에서 가장 효과적이었다. 생육 2개월 후 서광의 초장은 억제되었으나 생체중은 4 Gy와 2 Gy에서 증가되었고, 하우스모모타로의 초장은 12 Gy와 20 Gy에서, 생체중은 20 Gy와 4 Gy에서 가장 높은 증가효과를 보였다. 토마토 식물체의 고선량 피폭에 의한 생육장해는 사전 저선량 조사에 의해 감소되었는데 서광의 경우 2 Gy와 8 Gy 조사구에서 저항성이 높았으며 하우스모모타로의 경우 모든 저선량 조사구에서 저항성 효과가 나타났으며 특히 2 Gy와 12 Gy 조사구에서 높았다. Tomato (Lycopericum esculentum $M_{ILL}$ cv. Seokwang and cv. Housemomotaro) seeds were irradiated with the doses of $1{\sim}20$ Gy from $^{60}Co$ $\gamma$-ray source to investigate the effect of the low dose $\gamma$-ray radiation on the early growth and resistance to subsequent high dose of radiation. Germination rate of seeds irradiated with low dose $\gamma$-ray was enhanced in Seokwang cultivar but not in Housemomotaro cultivar. Seedling height increased in 4 Gy and 8 Gy irradiation group of both cultivars. Plant height of Seokwang cultivar was depressed in low dose irradiation group but fresh weight was increased in 2 Gy and 4 Gy irradiation group. In Housemomotaro cultivar, plant height increased in 12 Gy and 20 Gy irradiation group and fresh weight increased in 4 Gy and 20 Gy irradiation group. Growth inhibition of tomato plants by high dose radiation was noticeably reduced by pre-irradiation of low dose radiation. Resistance to subsequent high dose of radiation was enhanced in 2 Gy and 8 Gy Irradiation group of Seokwang cultivar and in 2 Gy and 12 Gy irradiation group of Housemomotaro cultivar.

      • SCOPUSKCI등재

        Methylovorus sp. strain SS1 DSM 11726의 메탄올 대사와 세포외 다당류 생합성

        김재성,김시욱,김영민,Kim, Jae. S.,Kim, Si W.,Kim, Young M. 한국미생물학회 1998 미생물학회지 Vol.34 No.4

        메탄올을 이용하여 성장하는 Methylovorus sp. strain SS1은 formaldehyde의 산화를 위한 linear route의 주효소인 $NAD^+$-linked formaldehyde dehydrogenase 및 $NAD^+$-linked formate dehydrogenas와 cyclic route의 주효소인 hexulose-6-phosphate synthase, glucose-6-phosphate isomerae, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase 등의 활성을 나타내었는데, cyclic route에 관여하는 효소의 활성이 상대적으로 더 높았다. 이 세균은 formaldehyde의 동화와 관련된 ribulose monophosphate 경로의 주효소와 Entner-Doudoroff 경로의 주효소 및 transaldolase 활성과 함께 세포외 다당류 합성과 관련된 phosphoglucomutase, UDP-glucose pyrophyosphorylase, mannose-6-phosphate isomerase의 활성도 나타내었다. 2.3 mM의 ammonium sulfate가 포함된 배지에서 성장한 세균은 7.6 mM의 ammonium sulfate가 포함된 배지에서 성장한 세균보다 더 많은 세포외 다당류를 생산하였지만 균체 수율은 낮았고, 6-phosphogluconate dehydrogenase와 phosphoglucomutase 및 UDP-glucose pyrophoshorylase의 활성은 높게 나타내었으나 6-phosphogluconate dehydratase/2-keto-3-deoxy-6-phosphogluconate aldolase의 활성은 낮았다. Melhylovorus sp. strain SS1 grown on methanol was found to show activities of key enzymes of the linear route, $NAD^+$-linked formaldehyde and formate dehydrogenases, and the cyclic route, hexulose-6-phosphate synthase, glucose-6-phosphate isomerase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase, for formaldehyde oxidation. The activities of the cyclic route enzymes were higher than those of the linear route enzymes. The bacterium also exhibited activities of the key enzymes of the ribulose monophosphate and Entner-Doudoroff pathways and transaldolase involved in the formaldehyde assimilation and the enzymes involved in the biosynthesis of extracellular polysaccharide. Cells grown in the presence of 2.3 mM ammonium sulfate were higher in the productivity of extracellular polysaccharide, but lower in the growth yield, than those grown in the presence 7.6 mM ammonium sulfate. The activities of 6-phosphogluconate dehydrogenase, phosphoglucomutase, and UDP-pyrophosphorylase in cells grown under nitrogen-limited condition were higher, but that of 6-phosphogluconate dehydratase/2-keto-3-deoxy-6-phosphogluconate aldolase was lower, than those in cells grown in the presence of sufficient amount of nitrogen source.

      • KCI등재

        OPC UA 기반 스마트팩토리 디지털 트윈 테스트베드 시스템 개발

        김재성,정석찬,서동우,김대기,Kim, Jaesung,Jeong, Seok Chan,Seo, Dongwoo,Kim, Daegi 한국멀티미디어학회 2022 멀티미디어학회논문지 Vol.25 No.8

        The manufacturing industry is continuously pursuing advanced technology and smartization as it converges with innovative technology. Improvement of manufacturing productivity is achieved by monitoring, analyzing, and controlling the facilities and processes of the manufacturing site in real time through a network. In this paper, we proposed a new OPC-UA based digital twin model for smart factory facilities. A testbed system for USB flash drive packaging facility was implemented based on the proposed digital twin model and OPC-UA data communication scheme. Through OPC-UA based digital twin model, equipment and process status information is transmitted and received from PLC to monitoring and control 3D digital models and physical models in real time. The usefulness of the developed digital twin testbed system was evaluated through usability test.

      • KCI등재

        답토양(畓土壤)에서 Strontium-90의 탈착(脫着)에 관(關)한 연구(硏究)

        김재성,임수길,이영일,정규회,Kim, Jae-Sung,Lim, Soo-Kil,Lee, Young-Il,Cheong, Kyu-Hoi 한국환경농학회 1987 한국환경농학회지 Vol.6 No.2

        방사성핵종중(放射性核種中) 식물(植物) 흡수율(吸收率)이 높은 $Sr^{90}$을 5개 답토양(畓土壤)에 처리(處理)하여 흡착(吸着)시킨 후 $Sr^{90}$ 탈착(脫着)에 대한 답토양(畓土壤)의 이(理)${\cdot}$화학적(化學的) 성질(性質)의 영향(影響)과 토양내(土壤內)에서의 형태별(形態別) 분포상태(分布狀態)을 조사(調査)하여 다음과 같은 결과(結果)를 얻었다. 1. 답토양(畓土壤)에 흡착(吸着)시킬 $Sr^{90}$의 대부분은 치환성(置換性)과 수용성(水溶性)이었고 특히 치환성함량(置換性含量)이 매우 높았고 그 량(量)은 $Sr^{90}$처리량(處理量)과 정비례적(正比例的)으로 증가(增加)하였으나 이에 반하여 Ca처리(處理)에 의해서는 감소(減少)되었다. 2. 답토양(畓土壤)에서의 $Sr^{90}$ 존재형태(存在形態)는 토양통(土壤統)에 따라서 차이가 있으나 평균적으로 치환성(置換性)이 59.3%, 수용성(水溶性)이 28.6%, 비치환성(非置換性)이 12.6%로 나타났다. 3. 비치환성형태(非置換性形態)로 전환되는 $Sr^{90}$량(量)은 Illite함량(含量)이 높고 Vermiculite함량(含量)이 적은 토양(土壤)에서 높았다. 4. 토양(土壤)에 흡착(吸着)된 $Sr^{90}$의 형태중(形態中) 수용성(水溶性)과 치환성(置換性) $Sr^{90}$의 탈착량(脫着量)은 토양(土壤) pH 와 치환성염기(置換性鹽基) 함량(含量)의 증가(增加)에 따라 감소(減少)하였고 유기물(有機物)과 점토함량(粘土含量)의 증가(增加)에 따라서는 증가(增加)하였다. The present study was carried out to determine the effect of the physical, chemical and mineralogical properties of paddy soil on the adsorption and desorption of $Sr^{90}$ from absorbed soils. The results obtained were as follows: 1. Most of the adsorbed $Sr^{90}$ was exchangeable and water soluble. $Sr^{90}$ extracted by ammonium acetate was very high compared to the water soluble fractions, and the ammount decreased with the increase of calcium application, but increased proportionally with the increase of $Sr^{90}$ treatment. 2. The distribution of $Sr^{90}$ in paddy soil depend on the soil type. Average-distribution rates of water soluble, exchangeable and non-exchangeable fractions of $Sr^{90}$ in the soils were 28.6%, 59.3% and 12.1% respectively. 3. The non-exchangeable from of $Sr^{90}$ was high in the soils of high illite and low vermiculite content. 4. The desorption of $Sr^{90}$ from adsorbed soils decreased with the increase of pH and ex-cations of the soils, but increased with the amount of organic matter and clay content in the soil.

      • KCI등재

        3요소(三要素) 및 석회처리(石灰處理)가 들깨의 지방조성(脂肪組成) 및 수량(收量) 구성요소(構成要素)에 미치는 영향(影響)

        김재성,이영일,신인철,Kim, Jae-Sung,Lee, Young-Il,Shin, In-Chul 한국토양비료학회 1992 한국토양비료학회지 Vol.25 No.2

        들깨의 시비조건(施肥條件)에 따른 수량(收量)과 함유량(含油量) 등을 조사한 결과는 다음과 같다. 1. 시비조건(施肥條件)에 따라 초장(草長), 가지수, 마디수, 화방수는 별 차이가 없었으며 가리시용(加里施用)은 들깨의 수량(收量)과 함유량(含油量)을 증가시키는 효과가 있었고,인산혼용(燐酸混用)은 들깨의 수량(收量)을, 석회(石灰)는 함유랑(含油量)을 증가시키는 효과가 있었다. 2. 가리(加里), 석회(石灰), 인산(燐酸)의 조합시비(組合施肥)는 종실(種實)을 크게 하나 함유량(含油量)은 낮았다. 3 들깨의 지방산조성(脂肪酸組成)은 lenolenic acid 가 64.5%로 최고(最高)였으며 다음이 oleic acid (14.0%), linoleic acid(13.8%) palmitic acid (5.4%). stearic acid (1.8%) 순이었고 시비조건(施肥條件)에 따른 특별한 분포경향을 보이지 않았다. 4. 들깨 종실(種實)의 회분중 무기성분(無機成分)은 K(21.3%), Ca(12.8%), Mg(8.2%), Fe(0.34%), Zn(0.15 %), Mn(0.1%), Na(0.08%) 순이었다. This experiment was carried out to investigate the effect of the application conditions of fertilizers on the seed yield and oil content of Perilla ocymoides L. The results were summarized as follows; 1. Plant height, number of nodes and branches, and number of clusters were not significantly affected by the application of fertilizers, but yield capacity and oil content of perilla increased by the potassium application. Phosphate application resulted in incerased seed yield and calcium application significantly affected the oil content. 2. The combined application of phosphate, potassium and calcium resulted in a big seed size but oil content was not high. 3. The fatty acid composition of perilla was not showed affected by the effect of application of fertilizer Unsaturated linolenic acid (64.5 %) was highest in the content of main fatty acid in perilla lipid followed by oleic acid (14.0 %), linoleic acid (13.8 %), palmitic acid (5.4 %) and stearic acid (1.8 %). 4. The contents of inorganic substances in seed ash of perilla showed the distribution pattern of K(21.3 %), Ca(12.8 %), Mg(8.2 %), Fe(0.34 %), Zn(0.15 %), Mn(0.10 %) and Na(0.08 %).

      • KCI등재

        Comparison of the Efficacy of Oral Capecitabine versus Bolus 5-FU in Preoperative Radiotherapy of Locally Advanced Rectal Cancer

        김재성,Jun Sang Kim,Moon June Cho,Wan Hee Yoon,Kye Sang Song 대한의학회 2006 Journal of Korean medical science Vol.21 No.1

        The effects of treatment with oral capecitabine vs. bolus 5-FU, administered concurrently with preoperative radiotherapy, were compared in the treatment of locally advanced rectal cancer (LARC). One hundred and twenty-seven patients with LARC received concurrent preoperative chemoradiation using two cycles bolus 5-FU (500 mg/m2/day) plus leucovorin (LV, 20 mg/m2/day) (Group I). Another LARC group received concurrent chemoradiation using two cycles 1,650 mg/m2/day of oral capecitabine and 20 mg/m2/day of LV (Group II, 97 patients). Radiation was delivered to the primary tumor at 50.4 Gy in both groups. Definitive surgery was performed 6 weeks after the completion of chemoradiation. A pathologic complete remission was achieved in 11.4% of patients in Group I and in 22.2% of patients in Group II (p= 0.042). The down-staging rates of the primary tumor and lymph nodes were 39.0/ 68.7% in Group I and 61.1/87.5% in Group II (p=0.002/0.005). Sphincter-preserving surgery was possible in 42.1% of patients in Group I and 66.7% of those in Group II (p=0.021). Grade 3 or 4 leucopenia, diarrhea, and radiation dermatitis were statistically more prevalent in Group I than in Group II, while the opposite was true for grade 3 hand-foot syndrome. Preoperative chemoradiation using oral capecitabine was better tolerated than bolus 5-FU and was more effective in the promotion of both down-staging and sphincter preservation in patients with LARC.

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