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Visfatin Stimulates Proliferation of MCF-7 Human Breast Cancer Cells
김재근,Eun Ok Kim,Bo Ra Jeong,Young Joo Min,박정우,Eun Sook Kim,Il Seong Namgoong,Young Il Kim,Byung Ju Lee 한국분자세포생물학회 2010 Molecules and cells Vol.30 No.4
Obesity, a condition characterized by increased fat content and altered secretion of adipokines, is a risk factor for postmenopausal breast cancer. Visfatin has recently been established as a novel adipokine that is highly enriched in visceral fat. Here we report that visfatin regulated prolifera-tion of MCF-7 human breast cancer cells. Exogenous ad-ministration of recombinant visfatin increased cell prolif-eration and DNA synthesis rate in MCF-7 cells. Further-more, visfatin activated G1-S phase cell cycle progression by upregulation of cyclin D1 and cdk2 expression. Visfatin also increased the expression of matrix metallopro-teinases 2, matrix metalloproteinases 9, and vascular en-dothelial growth factor genes, suggesting that it may func-tion in metastasis and angiogenesis of breast cancer. Taken together, these findings suggest that visfatin plays an important role in breast cancer progression.
분사열분해 CVD 법으로 증착된 YBCO 박막의 특성에 미치는 기판 온도의 영향
김재근,홍석관,유석구,조한우,김병주,안지현,홍계원,이희균,Kim, Jae-Geun,Hong, Suk-Kwan,Yu, Seok-Koo,Cho, Han-Woo,Kim, Byung-Joo,Ahn, Ji-Hyun,Hong, Gye-Won,Lee, Hee-Gyoun 한국초전도학회 2007 Progress in superconductivity Vol.9 No.1
YBCO films have been synthesized using a spray pyrolysis method. We used nitrates of Y, Ba, Cu as precursors. Deposition was made on $LaAlO_3$ (100) single crystal substrate by spraying the mist of aqueous precursor solution generated by a concentric nozzle. The distance between concentric nozzle and substrate was 15 cm. C-axis oriented films were obtained at deposition temperature of $740{\sim}800^{\circ}C$ and working pressure of 20 Torr. Oxygen partial pressure was 3 Torr and substrate was transported with the speed ranging from 0.23 cm/min to 0.7 cm/min by reel to reel. Scanning electron microscope (SEM) and X-ray diffraction (XRD) observation revealed that films are smooth and highly textured with (001) planes parallel to substrate. Highest critical current density (Jc) was $1.38\;MA/cm^2$ at 77K and self-field for the film with a thickness of $0.5\;{\mu}m$ prepared at a substrate temperature of $780^{\circ}C$ and $PO_2\;=3\;Torr$. The effect of temperature on the microstructure and YBCO phase formation will be discussed.
김재근,이선왕,김호진,홍계원,이희균,Kim, Jae-Geun,Lee, Sun-Wang,Kim, Ho-Jin,Hong, Gye-Won,Lee, Hee-Gyoun 한국초전도학회 2006 Progress in superconductivity Vol.7 No.2
Nickel and nickel-tungsten alloy were electroplated on a cold rolled and heat treated copper(Cu) substrate. 4 mm-thick high purity commercial grade Cu was rolled to various thicknesses of 50, 70, 100 and 150 micron. High reduction ratio of 30% was applied down to 150 micron. Rolled texture was converted into cube texture via high temperature heat treatment at $400-800^{\circ}C$. Grain size of Cu was about 50 micron which is much smaller compared to >300 micron for the Cu prepared using smaller reduction pass of 5%. 1.5 km-long 150 micron Cu was fabricated with a rolling speed of 33 m/min and texture of Cu was uniform along length. Abnormal grain growth and non-cube texture appeared for the specimen anneal above $900^{\circ}C$. 1-10 micron thick Ni and Ni-W film was electroplated onto an annealed cube-textured Cu or directly on a cold rolled Cu. Both specimens were annealed and the degree of texture was measured. For electroplating of Ni on annealed Cu, Ni layer duplicated the cube-texture of Cu substrate and the FWHM of in plane XRD measurement for annealed Cu layer and electroplated layer was $9.9^{\circ}\;and\;13.4^{\irc}$, respectively. But the FWHM of in plane XRD measurement of the specimen which electroplated Ni directly on cold rolled Cu was $8.6^{\circ}$, which is better texture than that of nickel electroplated on annealed Cu and it might be caused by the suppression of secondary recrystallization and abnormal grain growth of Cu at high temperature above $900^{\circ}C$ by electroplated nickel.
김재근,Kim, Jae-Geun 국립문화재연구소 1992 保存科學硏究 Vol.13 No.-
The Wando ship was discovered in 1984 in South Western Korea. The cargo of 30,000 celadons originated from the nearby kiln are thought to date from around the second half of the 11th century. The construction and timber species of the vessel indicate that it was built in Korea. The construcition is distinctive and unusual. There is no keel, rather five hevylongitudinal timbers which are pinned together with mortise and ten on joints. The side planking is attached to the bottom with chine planks. There are 5 strakes of the sides of the ship arranged in a rabbetted construction. All these features are associated with traditional ship construction method of old Korean ship.
김재근,민지은,박재우,Kim. Jae-Keun,Min. Jee-Eun,Park. Jae-Woo 한국방재학회 2008 한국방재학회 학술발표대회논문집 Vol.2008 No.1
To see the effect of magnesium on adhesion to natural zeolites, a series of batch tests were performed in this research. Mixed bacteria were sampled from the digestion tank at a local sewage treatment plant in Seoul. Magnesium-zeolites were synthesized by mixing natural zeolites with 0.096 M, 0.24 M, and 0.48 M of MgCl2 solution. For comparison, manganese and trivalent ferric zeolites were also prepared. Two grams of 0.2 mm <TEX>$\sim$</TEX> 0.3 mm sized zeolites(non-treated, Mg, Mn and Fe(III) treated zeolites) and 20 mL of water were mixed in a Corex 25 mL tube. Five milliliters of culture solution including bacteria was added to the tube. The tubes were equilibrated in a shaking incubator at mesophilic temperature <TEX>$(30{\pm}2^{\circ}C)$</TEX>. The bacterial concentrations were measured with a Microluminometer (New Horizons 3550i) and total organic carbon (TOC) spectrophotometer (Multi NC-3100).
분무 열분해 CVD법으로 이동 중인 LaAlO_3(100) 단결정 위에 증착시킨 YBCO 박막의 특성
김재근,홍석관,김호진,유석구,조한우,안지현,주진호,이희균,홍계원,Kim, Jae-Gun,Hong, Suk-Kwan,Kim, Ho-Jin,Yu, Seok-Koo,Cho, Han-Woo,Ahn, Jin-Hyun,Joo, Jin-Hoo,Lee, Hee-Gyoun,Hong, Gye-Won 한국초전도학회 2006 Progress in superconductivity Vol.8 No.1
YBCO films were deposited on a moving substrate by a spray pyrolysis method using nitrate aqueous solution as precursors. Deposition was made on $LaAlO_3$(100) single crystal substrate by spraying precursor droplets generated by a concentric nozzle. The cation ratio of precursor solution was Y:Ba:Cu=1:2.65:4.5. The distance between nozzle and substrate was 15 cm. Substrate was transported with a speed ranging from 0.23 cm/min to 0.5 cm/min. Films were deposited at the pressure ranging from 10 Torr to 20 Torr and the deposition temperature was ranged from $740^{\circ}C\;to\;790^{\circ}C$. Oxygen partial pressure was controlled between 1 Tow and S Torr. Superconducting YBCO films were obtained from $740^{\circ}C\;to\;790^{\circ}C$ with an oxygen partial pressure of 3 Torr. Scanning electron microscope(SEM) and X-ray diffraction(XRD) observation revealed that films are smooth and highly texture with(001) plans parallel to substrate plane. Highest Jc was 0.72 $MA/cm^2$ at 77K and self-field for the film with a thickness of 0.15 m prepared at a substrate temperature of $740^{\circ}C$ and $PO_2$=3 Torr.