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      • KCI등재

        Alloxan에 의한 HIT-T15 세포의 산화적 손상에 대한 매실 (Prunus mume Sieb. et Zucc.) 주정추출물의 세포보호효과

        김인혜,김종배,조강진,김재현,엄애선 한국자원식물학회 2012 한국자원식물학회지 Vol.25 No.2

        The present study was designed to examine the potential antidiabetic and antioxidant effect of ethanol extract from Prunus mume fruit (PME) against alloxan-induced oxidative stress in pancreatic -cells, HIT-T15. To evaluate the antidiabetic effect of PME, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliu bromide (MTT) cell proliferation assay,lactate dehydrogenase (LDH) release assay, NAD +/NADH ratio and insulin secretion were assessed. We also measured its antioxidant effect against alloxan-induced oxidative stress in the cells by assessing the levels of the antioxidant enzymes including superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx). The results of this analysis showed that alloxan significantly decreased cell viability, increased LDH leakage, and lowered NAD +/NADH ratio and insulin secretion in HIT-T15 cells. However, PME significantly increased the viability of alloxan-treated cells and lowered LDH leakage. The intracellular NAD +/NADH ratio and insulin secretion were also increased by 1.5~1.9-fold and 1.4-fold, respectively, after treatment with the PME. The HIT-T15 cells treated with alloxan showed significant decreases in the activities of antioxidant enzymes, while PME significantly elevated the levels of antioxidant enzymes. Based on these results, we suggest that PME could have a protective effect against the cytotoxicity and dysfunction of pancreatic β-cells in the presence of alloxan-induced oxidative stress. 본 연구는 췌장베타세포인 HIT-T15 세포를 이용하여매실주정추출물(PME)의 alloxan에 의한 산화스트레스로부터의 세포보호, 인슐린 분비능 및 항산화 효소 활성을 평가하였다. PME는 alloxan에 의해 유발된 산화스트레스로부터 세포를 보호하여 세포생존율을 증가시켰다. PME는세포막 손상지표인 LDH 방출을 억제하였고 NAD+/NADHratio를 유의적으로 증가시켜 세포사멸이 억제되어짐을 확인하였다. 또한 alloxan 단독처리군에 비해 250 ㎍/ml PME 처리군에서 인슐린 분비량이 유의성 있게 증가하였다. Alloxan과 PME를 동시에 처리하여 HIT-T15세포의 항산화효소 활성을 측정했을 때 산화스트레스에 의해 감소되었던 항산화효소 활성이 PME에 의해 보호되는 효과를 확인하였다. 이상의 연구결과로부터 PME는 세포괴사 및 DNA fragmentation을 억제하고 세포내 항산화효소 활성을 증가시켜 alloxan에 의해 유발된 산화스트레스로부터 췌장베타세포를 보호하고 이에 따른 인슐린 분비능 조절 효과가 있는 것으로 생각된다.

      • KCI등재

        국내에 도입된 Sedum album L.의 생육 특성 및 저토심 옥상 녹화 시스템에 관한 연구

        김인혜,허무룡,허근영,Kim In-Hye,Huh Moo-Ryong,Huh Keun-Young 한국조경학회 2005 韓國造景學會誌 Vol.33 No.5

        These studies were carried out (1) to investigate the growth characteristics of Sedum album L. in the field, (2) to propose a suitable shallow peen roof system for this plant, and (3) to evaluate plant growth in the proposed system over the long term. The growth characteristics, such as morphological properties, growth habit, shade tolerance, and flowering, were surveyed. In experimental shallow green-roof systems, the effects of drainage type, substrate type, and soil depth on plant growth were investigated. Then drought tolerance was investigated. After planting Sedum album L. in the proposed system survival rate, cover, and resistance to insects, heal and cold were evaluated for about 2 years. The results of these studies are summarized below. 1. In the field, the aboveground part of Sedum album L. did not die back during the winter. Plant height was 4$\sim$7 cm. Roots were distributed to a depth of 5$\sim$7 cm. Sedum album L. is a compact ground-cover plant that spreads vigorously. Shading condition of less than $30\%$ of full sunlight didn't cause any trouble, but shading conditions above $87\%$ made the shape of the shoots and leaves abnormal. The plant bloomed from June to August and had a rather large compound umbel of white, star-shaped flowers. 2. Two systems, a drainage-blend-10 cm soil depth and a reservoir$\cdot$drainage-blend-15 cm soil depth, performed best in terms of cover, fresh weight, and dry weight. The first has an advantage for green roofs because it is lighter than the latter. 3. In drainage-blend-10 m soil depth and modified reservoir · drainage-blend-10 cm soil depth system no plants died for about 4 months after stopping the irrigation. The visual quality of the latter system was above 5 for 4 months and that of the former was under 5 after 2 months. In the field, however, the drought tolerance of Sedum album L. grown in the former would be enough to withstand the dry season. Considering the urban ecosystem and the importance of healthy growth the modified reservoir $\cdot$ drainage-blend-10 cm soil depth system was finally recommended. This system was composed of a 4 cm thick drainage layer and drain outlets placed at a height of 2.5 cm. 4. In the proposed system, the survival rate was $100\%$, and there was no injury induced by insects and heat. The leaf density decreased a little in winter. Cover increased throughout the year. Sedum album L. was planted with a cover of 72$cm^{2}$ on 3 April 2003; on 16 June 2003 and 15 June 2004, cover was $132.66\pm$5.87 $cm^{2}$(1.8 times) and $886.98\pm$63.51 $cm^{2}$(12.3 times), respectively.

      • KCI등재

        대중매체와 개인매체를 통해 제공된 영양 관련 정보의 질 평가

        김인혜,최광남,배현주 한국식품조리과학회 2017 한국식품조리과학회지 Vol.33 No.4

        Purpose: The purpose of this study was to develop a strategy for the spread of proper information regarding nutrition through television, newspapers, and social network service (SNS). Methods: A total of 341 nutrition-related information offered through television (TV), newspapers, and SNS were evaluated according to 8 quality assessment items. Statistical analyses were conducted using the SPSS program (ver. 22.0) for ANOVA analysis and Duncan’s multiple range test. Results: The evaluation score of TV was significantly higher than those of the other media in terms of the provision of scientific evidence (p<0.01), clarity and concreteness of contents (p<0.001), sufficient explanation of the contents (p<0.05). Moreover, the evaluation scores of newspapers and SNS were significantly higher than that of TV in terms of the consistency in title and contents of the article (p<0.01), and no exaggeration or distortion on the contents (p<0.001). In the case of SNS, the evaluation score for a sufficient explanation of technical terms was significantly lower than those of TV and newspapers (p<0.001). As a result of a quality evaluation according to keywords, the evaluation score of information about sugar and sodium, and nutrition labeling was significantly higher than those of the other keywords in terms of clarity and concreteness of contents (p<0.001). The evaluation score of information regarding diet was significantly lower than those of the other keywords in 7 out of 8 items. Conclusion: To spread proper information regarding nutrition, it is necessary that practical information be provided with a sufficient explanation through a wide variety of media. Furthermore, consistent conduction of education for information users and providers is necessary.

      • KCI등재

        Analysis of Designation and Symbolic Meanings of Floral Emblems in South Korea as Elements of Garden Tourism and Design

        김인혜,박진실,최경옥 인간식물환경학회 2020 인간식물환경학회지 Vol.23 No.1

        This study was conducted to analyze the current state and symbolic meanings of floral emblem designation in local governments of South Korea. The scope of local governments subject to analysis was limited to special city, metropolitan city, province, self-governing province, city, self-governing city, county and district based on their administrative divisions. The floral emblems of local governments and their symbolic meanings as of 2019 were examined. A total of 44 plant species were designated as floral emblems. Many plant species with high designation frequency were included in Rhododendron spp., Rosa spp., Camellia spp., Magnolia spp., and Prunus spp.. Plant species with higher designation frequency tended to have more symbolic meanings. A total of 155 terms were used for the symbolic meanings assigned to all the designated floral emblems. The major symbolic meanings were relevant to material affluence or economic growth, community spirit, and personality generally required from local residents. Most of the plant species linked to the top 10 most frequently assigned terms in symbolic meaning were those of the top 10 most frequently designated floral emblems. In the case of floral emblems with high designation frequency, it was shown that they were linked with various symbolic meanings in order to grant identity with regional distinctiveness and differentiation, regardless of the symbolic meaning that the designated flowers generally have. However, the floral emblems with low designation frequency seem to have relatively strong physical or emotional relevance with local governments and thus are expected to have high utilization in regional branding and tourism marketing.

      • KCI등재

        『 발신집(發心集)』 의 「 스키(數寄)」 설화에 대한 일고찰

        김인혜 한국일어일문학회 1998 日語日文學硏究 Vol.33 No.1

        日本の中世は戰亂がつづき佛敎の無常觀の影響などで出家·遁世する人人が增えるようになった. 日本の中世の遁世者達は宗敎生活として佛道を修行しながら「數寄」にも熱中して日本の中世の文學に大きな影響を及ぼした. 遁世者達の生活の中での「數寄」は, そのまま彼らの文學の中に現われて遁世者による隨筆文學や佛敎說話集の發達も著しかった. このような遁世者の「數寄」の槪念が現われている『發心集」の數寄說話には, 前の時代の「袋草紙」や「今鏡』とほとんど同じ逸話が載せられている. 本考では「發心集」の數寄說話を通じて前の時代と區別される中世の「數寄」の槪念及び宗敎との關係を考察して見た.「發心集」の數寄說話を內容によって 1)「數寄者」としての人柄と資質, 2)「數寄」の樣相, 3)「數寄」と佛道との關係に分けて調べて見た. 考察の結果を見ると, 1)「數寄者」としての人柄と資質には, 第5卷8話「中納言顯基, 出家·籠居の事」と第6卷6話「侍從大納言幼少の時, 驗者の改請を止むる事」がある. その中で第5卷8話は『袋草紙」上卷「雜談」にも類似な話が載せられている. これを比べて見ると『袋草紙』では後一條天皇の死後すべての權力·榮華等をすてて出家すると言う忠臣としての顯基を描いている.『發心集」は, 『袋草紙』とほぼ同じ內容だが, 名利を越える, 世の濁りに心をそめない点に重点をおいていた.『發心集』に現われた數寄說話は「數寄」を行なう「數寄者」の人柄と資質が重要になって人としての道理·情などを持ち, 比の世の榮えを好まず名利を越えた 淸い心を持つことなどを「數寄者」の資質と見て, これから展開される數奇說話に前提的な役割を果たしている. 2)「數寄」の樣相には, 第6卷7話「永秀法師, 數奇の事」と第6卷8話「時光·茂光, 數奇天德に及ぶ事」がある. その中で第6卷8話は『今鏡』昔語第九「賢き道道」にも同一な話が載せられている. これを比べて見ると『今鏡』では二人の音樂に對する熱情より王の心有りに重みをおき, 『發心集』では風雅の持つ絶對的な自由に重みをおいて音樂に熱中する二人の「數奇」が世の主從關係を越えるという純粹な「數寄」を現わしてしいる.「發心集」の數寄の樣相は「數寄」の對象への專念, すなわち風流の心を持ち, 淸い心で「數寄」だけを追求することを樂しみとする. 純粹な「數寄」の樣相を描いて典型的な數寄說話を展開した. 3)「數寄」と佛道との關係には, 第6卷9話「寶日上人, 和歌を詠じて行とする事 井蓮如, 讚州崇德院の御所に參る事」と第7卷5話「太子の御墓覺能上人, 管絃を好む事」がある. 第6卷9話は 四人の話で構成されているがその中で惠心僧道の逸話は『袋草紙」上卷「雜談」にも同一の逸話がある. その差は和歌と佛敎との關係の違いで, 『袋草紙』では和歌が觀念の助緣として, 「發心集』では和歌と佛道の一致として現われている. 和歌を詠じたり, 管絃を行なったりする「數寄」その自體が修行の一方法として認められたところで「數奇」と佛道の融化を現わした. このように『發心集』に現われた數寄說話は「數寄」を行なう者の資質等を重要視して, 名利を越えた, 純粹な「數奇」の姿を見せ, 「數寄」と佛道との融化と言う前の時代と違う中世の「數寄」の槪念を明確にした.

      • KCI등재

        진주 남가람공원의 바닥분수에 대한 이용자의 행태 및 만족 평가

        김인혜,허현철,허근영 인간식물환경학회 2009 인간식물환경학회지 Vol.12 No.3

        수조가 지하에 매설되어 배관이나 배선이 노출되지 않고 바닥면에서 물이 분사되는 형태의 분수인 바닥분수는 일반적으로 공공 정원의 광장에 도입되어 수경관을 접할 기회를 제공하고 분수가 가동되지 않을 때에는 광장의 기능을 하는 다목적 옥외 공간을 제공하고 있다. 본 연구는 이용후 평가를 통하여 바닥분수를 위한 효과적인 설계와 관리 방안을 제안할 목적으로 수행되었다. 진주 남가람 공원 중앙광장에 조성된 바닥분수가 도면 및 현장조사, 관찰, 인터뷰, 설문조사를 통하여 이용자의 행태와 만족도를 중심으로 평가되었다. 이용자의 주요 행태는 ‘물놀이’, ‘더위 식히기, ’분수 감상‘, ’산책‘이었다. 바닥분수의 이용은 남가람 공원 중앙광장의 전체 이용에 유의성 있는 영향을 미치고 있었다. 이용 만족도는 ’안전성‘, ’안전 관리자의 배치‘, ’용수의 음용 적합성‘을 제외하고는 전반적으로 높은 수준을 나타내었다. 종합적인 만족도에 영향을 미치는 변수들은 ’분수의 기능적 적합도‘, ’용수의 정기적 교체‘, ’놀이 공간으로서의 적합성‘이었다. 바닥분수에서의 개선 및 보강 사항으로는 ’미가동 기간 중의 프로그램‘, ’안전 시스템의 보완‘, ’프로그램 분수‘ 등에 대한 이용자들의 요구도가 높았다. Pop-jet fountain is a type of fountain in which the pond is laid under the ground and water spouts out from the ground without exposing its piping or wiring. It has been generally applied to squares at public gardens providing more opportunities to contact water features and multipurpose outdoor space which is used as a public square during the time when the fountain is not operated. This study was carried out to suggest effective design and management for pop-jet fountain through a Post-occupancy Evaluation(POE). The pop-jet fountain installed on the main square at Namgaram Park in Jinju was evaluated with regards to user's behavior and satisfaction by plan investigation, observation, interview, and questionnaire. The users' main behaviors included 'playing in the water', 'avoiding heat', 'looking at the fountain' and 'taking a walk'. The use of pop-jet fountain had a significant influence on the total use of the main square in Nagaram park. The satisfaction of the users showed high level except in 'safety', 'disposition of supervisors', and 'fitness for drinking the water'. The variables affecting the overall satisfaction were 'functional suitableness of the fountain', 'regular change of the water', and 'convenience of using as a playing space'. Improvements for the fountain and the main square such as 'programs during the non-operating period', 'supplementation of safety systems', and 'programed operation of the fountain' were requested by users.

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