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효모 발현 시스템을 이용 PAP(Pokeweed Antiviral Protein) 유전자의 세포독성 연구
김선원,박성원,강신웅,이영기,이종철,최순용,이청호 한국연초학회 2001 한국연초학회지 Vol.23 No.2
PAP-I cDNA was synthesized from total RNA of Phytolacca americana leaves by RT-PCR, and then subcloned to recombinant vector pBluescript II SK-. Using PCR with primers designed in our laboratory, we could get the 9 deletion mutant PAP-I cDNA fragments. The first of the fragments was deleted by 66bp from immature N-terminal and then the rest were deleted by 90bp sequentially. Sequentially deletion mutant PAP-I cDNAs were inserted to pAc55M, on down-stream of gall promoter. Recombinant pAc55M was transformed to yeast cells, psy1 and the cells were spreaded on SC_urn-/glucose plate media. Colonies on SC_ura-/glucose plate were streaked on the same position of SC_ura-/glucose and SC_ura-/galactose plate, and we selected colonies growing on both plates, which carry non-cytotoxic deleted mutant PAP-I cDNA. We selected 4 deletion mutant PAP-I cDNAs which have not cytotoxicity.
담배식물체에 PMT(Putrescine N-Methyltransferase) antisense RNA유전자 도입
김선원,박성원,이정헌,이청호,류명현,복진영,김도훈,최순용 한국연초학회 2003 한국연초학회지 Vol.25 No.1
Transgenic tobacco plants were selected by using the transformation of putrescine N-methyltransferase(PMT) gene, the key enzyme in diverting polyamine metabolism towards the biosynthesis of nicotine. PMT was fused in reverse orientation to the CaMV 35S promoter of the plant expression vector pBTEX(pPAB3) to produce tobacco plants of low nicotine content. To compare nicotine content, only pBTEX vector and PMT gene which was fused in forward orientation to the CaMV 35S promoter(pPAB2) were also transformed to the leaf tobacco plants(Nicotiana tabacum cv. NC82 and N. tabacum cv. Br2l). The presence of sense- and antisense-PMT gene, and pBTEX vector in the transgenic plant was confirmed by genomic PCR.
김선원,송병기,이성재,Kim, Sun-Won,Song, Byung-Ki,Lee, Song-Jae 한국광학회 2002 한국광학회지 Vol.13 No.4
LED 램프의 기본 설계이론을 제시하였다. 설계된 LED 램프에 사용된 reflecting cup과 렌즈는 몇몇의 설계 변수에 의하여 다양한 형태로의 모양의 변화가 가능하다는 특징을 갖는다. Monte Carlo 기법을 이용하여 설계된 램프를 해석한 결과 다양한 응용분야에서 요구되는 빔 패턴특성을 손쉽게 구현할 수 있음을 확인할 수 있었다. The fundamentals of light-emitting diode lamp design are presented. The shapes of the reflecting cup and lens employed in LED lamps can be flexibly adjusted, over a relatively large range, by using a few parameters. The analysis of the designed lamps by Monte Carlo photon simulation shows that diverse far-field beam patterns required in various application areas can be achieved.