계측분석을 위한 연약지반 거동의 이해

김상환,김상환 대한지질공학회 2003 지질공학 단기강좌 Vol.2003 No.-

Propylene 산화반응에서 촉매내 전달 현상의 영향

김상환,이인철,박형상 한국화학공학회 1988 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.26 No.5

Propylene 부분 산화용 tin-antimony oxide계 공업용 촉매에서 촉매내 물질전달 현상이 반응에 미치는 영향을 다공성 매질내의 물질전달에 관한 Dusty-Gas model과 Feng-Stewart model을 이용하여 검토하였다. 본 촉매반응의 경우 촉매내에 multiple solution은 생기지 않았으며, 촉매 내부 전달 현상에 의하여 propylene의 acrolein으로의 선택성은 감소하였다. 위 두 model이 서로 다른 basis를 갖고 있는데 반하여, 기공 크기가 uniform하고 촉매내에서 viscous flow가 없는 경우에는 같은 결과를 보여줌을 알 수 있다. The influence of internal diffusion on the partial oxidation of propylene on the tin-antimony oxide based catalyst has been studied. For this study, two flux relations in the porous medium (Dusty-Gas model and FengStewart model) have been adopted. In this catalytic reaction system, multiple solution problem has not happened and the internal diffusion decreases the selectivity of propylene to acrolein. The two models differ conceptually, but it has been known that they give the same result for the catalytic reaction system of uniform pore size and no net viscous flow.

칡소의 MC1R의 유전자형에 따른 교배 조합이 자손의 모색과 유전자형 변이에 미치는 영향

김상환,정경섭,이호준,백준석,정덕원,김대은,윤종택 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.3

Bovine coat color is decided by the melanocortin receptor 1 (MC1R) genotype mutation and melanogenesis. Specially, in the various cattle breeds, dominant black coat color is expressed by dominant genotype of ED, red or brown is expressed in the frame shift mutation of recessive homozygous e by base pair deletion and wild type of E+ is expressed in various coat colors. However, not very well known about the effected of MC1R genotype mutation on the coat color through family lines in KBC. Therefore, this study were to investigate effect of MC1R genotype mutation on the coat color, and to suggest mating breed system in accordance with of MC1R genotype for increased on brindle coat color appearance. Parents (sire 2 heads and dam 3 heads) and offspring (total : 54 heads) from crossbreeding in KBC family line with the MC1R genotype and phenotype records were selected as experimental animals. The relationship between melanocortin 1 receptor (MC1R) genotypes expression verified by PCR-RFLP, and brindle coat color appearance to the family line of the cross mating breed from MC1R genotype pattern was determined. As a result, 4MC1R genetic variations, E+/E+ (sire 1), E+/e (sire 2 and dam 3), E+/e with 4 bands of 174, 207 and 328 bp (dam 1) and E+/e with 3 bands of 174, 207, 328 and 535 bp (dam 2) from parents (sire and dam) of KBC. However, 3 genetic variations, e/e (24%), E+/E+ (22%) and E+/e (56%) were identified in offspring. Also, brindle coat color expressrated was the e/e with the 0%, E+/E+ with 67% and E+/e with 77% from MC1R genotype in offspring on the cross mating of KBC. Furthermore, when the sire had E+/e genotype and the dam had E+/E+ with the 3 bands or E+/e genotype, and both had whole body-brindle coat color, 62% of the offspring had whole body-brindle coat color. Therefore, the seresults, the mating system from MC1R genotype patterns of the sires (E+/e) and dams (E+/E+ with the 3 bands or E+/e) with brindle coat color may have the highest whole body-brindle coat color expression in their offspring.

이미지에서 線으로

김상환 한국외국어대학교 철학문화연구소 2006 인문학연구 Vol.11 No.-

Detection of Matrix Metalloprotease-9 and Analysis of Protein Patterns in Bovine Vaginal Mucus during Estrus and Pregnancy

김상환,백준석,이호준,민관식,이득환,윤종택 사단법인 한국동물생명공학회 2012 한국동물생명공학회지 Vol.27 No.2

To investigate the biochemical nature of changes in vaginal physiology during estrus and pregnancy, we examined the cytology and viscosity, and monitored the protein expression profile in vaginal mucus during estrus and pregnancy. The viscosity progressively decreased from estrus to pregnancy. Cell type analysis revealed that white blood cells progressively increased from estrus to pregnancy, while red blood cells progressively decreased during pregnancy. The cornification index (CI) was higher in estrus than in pregnancy. Protein mass spectrumetry identified the presence of ribosome-binding protein 1, GRIP 1 (Glutamate receptor-interacting protein 1)-associated protein 1, DUF729 (Domain of unknown function729) domain-containing protein 1, prolactin precursor, dihydrofolatereductase, and MMP (Matrix metalloprotease)-9 in vaginal mucus. MMP-2 and MMP-9 proteins in the vaginal mucus were active throughout estrus and gestation, as measured by a gelatinase assay, but most abundant in the vaginal mucus on day 0 of estrus. Results from ELISA of MMP-2 and MMP-9 were in accordance with the gelatinase assay. In light of the crucial role of metalloproteinases in extracellular matrix remodeling, the level of MMP-9 in vaginal mucus might be useful as an indicator of estrus and pregnancy to increase the efficiency of reproduction.

터널 리스크 평가 기법의 적용성에 대한 연구

김상환,이충환,Kim, Sang-Hwan,Lee, Chung-Hwan 한국터널지하공간학회 2007 한국터널지하공간학회논문집 Vol.9 No.1

불확실한 지반의 상태는 항상 터널 건설에 있어서 많은 문제점을 일으킨다. 그러므로 새로운 터널의 건설에 앞서 위험에 대한 평가가 필요하다. 이 논문은 수정된 안정계수를 이용한 간편 위험도 평가 시스템인 URI(Underground Risk Index)를 제안하였다. URI의 평가요소들은 각 요소의 등급에 따라 점수화하여 분석함으로서 설계단계에서 위험 가능성에 대한 평가에 이용이 가능하도록 하였으며, 기존의 Interaction Matrix에서 8개의 설계 요소 중(RQD, 일축압축강도, 풍화도, 안정계수, 토피고, 지하수위, RMR, 투수계수)안정계수 산정방법을 수정하여 보다 신뢰성을 높였다. 또한 현장적용에 대한 신뢰성을 검증하기위하여 URI 시스템을 실제현장에 적용하였다. Unexpected ground conditions have always been a major problem for the construction of tunnel. Therefore, it is necessary to evaluate the risk capacity before and/or during construction of new tunnel. This paper presents the simplified risk assessment system using modified stability number (N), namely Underground Risk Index (URI) system, to evaluate the tunnel risk possibility in the design stage. URI is a scoring system for risk possibility by rating the each appraisal elements. The modified stability number (N) which is one of risk factor in the Interaction Matrix parameters such as RQD, UCS, weathering, overburden, stability number, ground water-table, RMR, permeability and so on, is used in the system. In addition, the case study is performed in order to verify the applicability of URI-system in practice.

젖소 BCS관리의 중요성

김상환 한국종축개량협회 2000 젖소개량 Vol.2000 No.10

석회암 공동발달유형에 따른 터널지보패턴의 표준화에 대한 연구

김상환,Kim, Sang-Hwan 한국터널지하공간학회 2003 터널기술 Vol.5 No.3

일반적으로 석회암 공동발달지역의 터널지보는 터널의 안정성을 좌우하는 매우 중요한 요소중에 하나이다. 이를 위하여 설계단계에서 석회암 공동발달지역과 같은 불확실성 지반조건에서 터널의 지보패턴에 대하여서는 용이하게 결정 할 수 없을 것이다. 따라서 석회암 공동발달지역에서의 일반적으로 제시할 수 있는 터널지보패턴에 대한 표준화가 요구되어진다. 이 논문은 석회암공동발달지역에서의 터널지보패턴의 표준화기법에 대한 연구 결과를 제시하였다. 이 연구는 국내의 석회암 공동발달지역의 불확실한 지반조건에서의 터널지보 System에 대한 간편화 기법에 대하여 기술하였다. 특히, 석회암공동발달지역에 대한 지반의 등급화기법 뿐만 아니라 이에 따른 터널지보패턴 간편화 기법에 대하여서도 제시하였다. 또한, 석회암 공동지반등급과 지보패턴별 요구되는 보강 및 보조공법에 대하여서도 상세 서술하였다. 이 연구를 수행하기 위하여 석회암 공동이 발달되어 있는 ${\bigcirc}{\bigcirc}$지역에 계획되었던 터널에 대하여 실질적으로 설계과정을 통하여 지보패턴의 형식선정기법의 적용에 대한 연구를 실시하였다. 이 연구 결과를 통하여 향후 석회암 공동발달지역 뿐만 아니라 이와 유사지반에서의 터널지보 설계에 적용하므로써 터널기술향상을 도모할 것이다. In karst formation area, the tunnel support system is an important factor for the tunnel safety during operation. It is also not easy to determine the tunnel supporting system in the design stage. Therefore, it is necessary to standardize the tunnel supporting system in uncertain ground condition. This paper presents the standardization of the tunnel supporting systems to be adopt in karst formation. For the tunnel planned in the project area, karst features and the expected scenarios in the tunnel area were developed based on the results of the geological and geotechnical assessment. In order to provide specific supporting system and construction details for a wide range of possible karst features, the generalized typical support systems are developed according to the classification of karst features. In addition, the initial support systems and construction sequence for each karst feature are also presented in this paper.

TES 보존액이 미니돼지 동결 융해 정자의 생존성 및 성상에 미치는 영향

김상환,강현아,이명섭,서강석,윤종택 韓國受精卵移植學會 2012 한국동물생명공학회지 Vol.27 No.1

The objective of this study is to estimate the effect of adding TES to LEY and FGE freezing extender for the sperm viability, acrosomal morphology and DNA fragmentation from miniature pig sperm, we evaluated sperm characteristics in TFGE, TLE and LEY with various thawing condition ( for 20 sec, 45 sec and for 5 sec, respectively), and in different concentration of glycerol at 1%, 1.5%, 3%. The sperm viability and normal acrosome intact(NAI) in TFGE (Viability : , NAI : ), TLE (, ) extender significantly(p<0.05) increased than that in LEY (, ) extender thawed at for 5 sec. According to the results from glycerol concentration, the viability and NAI of miniature pig sperm in 1.5% glycerol TLE (, ) was highest among the experimental groups. In accordance with this, DNA fragmentation rates was the lowest in TLE () while that in LEY () is the highest. Therefore, these results suggest that TLE extender method for freezing- thawing of miniature pig sperm increased the viability after thawing.

Age-Associated Increasing of MCP-1 in Adults

김상환,임지애 대한의생명과학회 2007 Biomedical Science Letters Vol.13 No.3