http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
권석형 ( Suk Hyung Kwon ),김춘년 ( Choon Nyeon Kim ),김철용 ( Chul Yong Kim ),권석태 ( Suk Tae Kwon ),박기문 ( Ki Moon Park ),황보식 ( Sik Hwangbo ) 한국식품영양학회 2003 韓國食品營養學會誌 Vol.16 No.1
버섯 균사체로부터 분리한 단백다당체의 Th1 cell 증식효과 및 각종 암세포에 대한 세포독성을 조사하였다. 단백다당체 시료는 영지버섯, 아가리쿠스, 표고버섯, 운지버섯, 그리고 상황버섯을 100℃에서 3시간 열수 수출하는 방법으로 조제하였다. Th1 cell의 세포 증식을 조사한 결과, 10mg/ml 농도에서 모든 시료가 40% 이상 억제하는 효과가 있는 것으로 나타났다 7종류의 암세포를 이용한 암세포 생존율을 조사한 결과, 1mg/ml 농도에서 P388D1와 L1210에서 아가리쿠스는 2.4% 와 39.7%, 표고버섯은 48.4%와 52.5% 의 생존율을 보였다. Sarcoma 180 으로 복수암을 유발시킨 마우스에게 아가리쿠스와 표고버섯으로부터 추출한 단백다당체를 섭취시킬 경우 생존율이 27 ~ 40%까지 유의적으로 증가하는 것으로 나타났다. The purpose of this study is to observe the effect of protein-bound polysaccharide (PBP) on proliferation of Th1 cells and cytotoxicity of cancer cell. Mushrooms (Ganoderma lucidum, Agaricus blazei, Lentinus edodes, Coriolus versicolor and Phellinus linteus) were fractionated by 100℃ hot water for 3hr. PBP was stimulated and proliferated Th1 cells most at 10 mg/ml concentration and the percentage of cell proliferation was 40%. It was estimated cytotoxicity of PBP against 7 kinds of cancer cell lines. Antitumor activities of Agaricus blazei against P388D1 and L1210 (tumor cell lines) were 2.4% and 39.7% survival rate, and Lentinus edodes was 48.4$ and 52.5% survival rate, respectively. PBP mixtures of Agaricus and Lentinus edodes prolonged (27 ~ 40%) significantly the survival rate of mice intraperitoneally implanted with sarcoma 180.
김철호,권석태,이대실,Kim, Cheorl-Ho,Kwon, Suk-Tae,Lee, Dae-Sil Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.4
B. circulans 유래의 Cl-amylase는 전형적인 분비효소 단백질이다. 본 효소 단백질의 세포내 수송기구를 해명하고 분비에 필요로 하는 signal sequence 의 기능을 확인하기 위하여 signal sequence의 16번째 아미노산 잔기(serine)를 proline(${\alpha}$-helix breaker)과 threonine으로 In vitro mutagenesis를 통해 바꾸었다. Recombinant wild형(serine)과 mutation된 Cl-amylase(proline과 threonine) 유전자 각각을 대장균에 도입하여 세포내의 단백질 수송을 효소활성과 변역학적 방법으로 검토한 결과, proline-mutant형 이 wild형의 약 5% 정도로 periplasmic space에서 검출되었으며, 분자량에 있어서는 약 3,000 dalton의 차를 보였다. 그러나, threonine-mutant는 wild(serine) type의 경우와는 차이가 검출되지 않았다. 상기의 결과들로, 대장균에서의 이종유전자 산물의 성공적인 processing과 protein transportation을 확인, 해명하게 되였다. The possible signal sequence capable of transporting the Cl-amylase from B. circulans has been analyzed with in vitro mutagenesis techniques. A residue in the $NH_2$-terminal region near to the postulated cleavase site was changed by site-directed mutagenesis from a serine into proline and threonine. Comparison of Cl-amylase acitivity outside and inside the cell in strains containing the cloned wild type and mutagenised genes showed that this single amino acid prevents largely the translocation of the enzyme in the periplasmic space: in transformed E. coli the proline -mutant Cl-amylase showed 5% secretion of wild type Cl-amylase and threonine-mutant Cl-amylase.
Recombinant Cl - amylase 의 세포내 수송에 있어서 Signal sequence 의 역할
김철호,권석태,이대실 ( Cheorl Ho Kim,Suk Tae Kwon,Dae Sil Lee ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.4
The possible signal sequence capable of transporting the Cl-amylase from B. circulans has been analyzed with in vitro mutagenesis techniques. A residue in the NH₂-terminal region near to the postulated cleavase site was changed by site-directed mutagenesis from a serine into proline and threonine. Comparison of Cl-amylase acitivity outside and inside the cell in strains containing the cloned wild type and mutagenised genes showed that this single amino acid prevents largely the translocation of the enzyme in the periplasmic space: in transformed E. coli the proline -mutant Cl-amylase showed 5% secretion of wild type Cl-amylase and threonine-mutant Cl-amylase.
pBRG-4를 이용한 Metarhizium anisopliae의 형질전환
이동규,예완해,황철원,권석태,강선철,Lee, Dong-Gyu,Yeh, Wan-Hae,Hwang, Cher-Won,Kwon, Suk-Tae,Kang, Sun-Chul 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.2
작물 병해충에 대한 무공해 미생물 농약 개발의 일환으로 곤충 병원성 곰팡이 Metarhizium anisopliae의 분자생물학적 육종을 위해 이 균주의 형질전환계를 확립하였다. M. anisopliae의 원형질체를 제작하여 benomyl 약제에 대하여 저항성을 나타내는 Aspergillus flavus 유래의 ${\beta}-tubulin$ 유전자를 갖는 pBRG-4 plasmid DNA를 polyethylene glycol 방법으로 형질 전환하였다. 이 형질전환은 pBRG-4 DNA $50\;{\mu}g$당 10개의 효율로 이루어졌으며, 그 결과 야생형 균주들의 $2.5\;{\mu}g/ml$ 농도의 benomyl 존재 하에서도 성장이 억제되는데 반하여 선발된 형질전환체들은 $5.0\;{\mu}g/ml$ 농도의 benomyl 함유 배지에서도 잘 성장하였다. 또한 이 형질전환체들의 chromosomal DNA를 분리하여 Southern 분석한 결과 ${\beta}-tubulin$ 유전자가 homologous recombination에 의하여 M. anisopliae의 genome속에 삽입 되었음을 확인하였다. We have established a transformation system for entomopathogenic fungus, Metarhizium anisopliae, in order to develop mycoinsecticide by recombinant DNA techniques. Protoplasts of M. anisopliae would be transformed to a benomyl-resistant by introducing pBRG-4 plasmid DNA, which contains a ${\beta}-tubulin$ gene of Aspergillus flavus conferring resistance to benomyl and a pyr4 gene of Neurospora crassa, in the presence of 5% polyethylene glycol and 10 mM calcium chloride. Transformants occuring at a frequency of 10 colonies per $50\;{\mu}g$ pBRG-4 DNA grew on the $5\;{\mu}g/ml$ concentrations of benamyl, while the wild type was inhibited by $2.5\;{\mu}g/ml$. From the Southern analysis using genomic DNAs isolated from M. anisopliae transformants, the positive signals suggested that the ${\beta}-tubulin$ gene had integrated in the M. anisopliae genome by homologous recombination.
곤충 병원성 곰팡이 Beauveria bassiana로부터 Protease의 정제와 특성
고휘진,김현규,김범기,강선철,권석태 ( Hwi Jin Ko,Hyun Kyu Kim,Beom Gi Kim,Sun Chul Kang,Suk Tae Kwon ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.5
Extracellular protease (bassiasin I), from the culture filtrate of entomopathogenic fungus Beauveria bassiana ATCC7159, was successively purified by precipitation with ammonium sulfate followed by DEAE-Sephadex A-50, CM-cellulose and Hydroxyapatite column chromatography. A typical procedure provided 41-fold purification with 13.6% yield. The molecular weight of the purified protease (bassiasin I) was found to be approximately 32,000 by SDS-PAGE. Isoelectric-focusing analysis of the enzyme showed a pI of 9. 5. NH₂-terminal sequence of the protease showed homology with those of the fungal proteases. The enzyme has an optimal pH for activity at 10.5 and is stable over pH 5.0-11.0. The maximum activity of the enzyme was at 60-65℃, and approximately 20% activity remained at 60℃ after 120 min. The protease was inhibited by phenylmethylsulfonyl fluoride (PMSF) and diisopropyl fluorophosphate (DIFP).
Thermus caldophilus GK24로부터 내열성 $\beta$-galactosidase의 최적 생산
유진상,김현규,인만진,김민홍,권석태,Yoo, Jinsang,Kim, Hyunkyu,In, Man-Jin,Kim, Min-Hong,Kwon, Suk-Tae 한국미생물 · 생명공학회 1997 한국미생물·생명공학회지 Vol.25 No.3
Thermus caldophilus GK24 was selected as sources of thermostable $\beta$-galactosidase from a survey of genus Thermus. T. caldophilus GK24 (Tca) $\beta$-galactosidase was found to be inducible. The enzyme was optimally active at 75$\circ$C. Enzyme induction was achieved by addition of lactose, galactose and cellobiose to basal media. The addition of glucose to culture media had a repressive effect on further enzyme synthesis. T caldophilus GK24 was tested for production of $\beta$-galactosidase by addition of various concentration of lactose, galactose and cellobiose to standard media. Cellobiose was found to be effective for the $\beta$-galactosidase induction. The optimal induction medium for production of $\beta$-galactosidase was composed of 0.2% cellobiose, 0.3% bactotryptone, 0.3% yeast extract, basal salts and Tris/HCI(pH 7.8). The activity of the enzyme in the optimal induction medium increased nearly 16.5-fold compared to the standard medium. Tca $\beta$-galactosidase was detected when cell extracts was subjected to electrophoresis in a nondenaturing polyacryamide gel and stained for activity with 6-bromo-2-naphtyl-$\beta$-D-galactopyranoside(BNG).
pBRG-4 를 이용한 Metarhizium anisopliae 의 형질전환
이동규,예완해,황철원,권석태,강선철 ( Dong Gyu Lee,Wan Hae Yeh,Cher Won Hwang,Suk Tae Kwon,Sun Chul Kang ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.3
We have established a transformation system for entomopathogenic fungus, Metarhizium anisopliae, in order to develop mycoinsecticide by recombinant DNA techniques. Protoplasts of M. anisopliae would be transformed to a benomyl-resistant by introducing pBRG-4 plasmid DNA, which contains a β-tubulin gene of Aspergillus flavus conferring resistance to benomyl and a pyr4 gene of Neurospora crassa, in the presence of 5% polyethylene glycol and 10 mM calcium chloride. Transformants occuring at a frequency of 10 colonies per 50 ㎍ pBRG-4 DNA grew on the 5 ㎍/㎖ concentrations, of benamyl, while the wild type was inhibited by 2.5 ㎍/㎖. From the Southern analysis using genomic DNAs isolated from M. anisopliae transformants, the positive signals suggested that the β-tubulin gene had integrated in the M. anisopliae genome by homologous recombination.