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권민희,염승호 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.2
Biodiesel production from microalgal biomass by a one-step direct process was investigated, and optimal conditions for this process were determined. The one-step direct process comprises the extraction of lipids from microalgae and the simultaneous transesterification of the unpurified lipids with methanol. The lipid content of Nannochloropsis sp. KMMCC 290, which was cultivated in a raceway pond, was determined to be 16.1% by pretreatment using a bead beater and conventional lipid measurement by the Bligh and Dyer method. Among the combinations of catalysts and organic extraction solvents evaluated, sulfuric acid with chloroform showed the highest lipid extraction efficiency and biodiesel conversion. Contrary to previous reports, the effect of shaking on lipid extraction and biodiesel conversion during biodiesel production was not significant. The optimal temperature and reaction time were determined to be 70°C and 90 min, respectively. Under the optimal conditions, the biodiesel conversion based on the total lipid content and the convertible lipid content was 84.2 and 96.7%, respectively. The highest biodiesel production was 155.7 mg/g-dry cell.
권민희,염승호 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
To decrease the cost of biodiesel production from microalgae, biodiesel was produced from wet marine microalgae by a one-step direct process. In this process, simultaneous lipid extraction from the wet microalgae and transesterification of the lipid with methanol was conducted. Among the combinations of catalysts and organic extraction solvents that were evaluated, sulfuric acid and chloroform represented the optimum combination. The degree of water content for wet microalgae significantly influenced biodiesel production: in the presence of 348.4% water content in intact wet microalgae, lipid extraction efficiency (LEE) was 73.2% and biodiesel conversion (BC) was 50.5%, but at a lower water content of 185.7%, LEE increased to 84.7% and BC to 69.9%. Increasing the amount of chloroform by 50% relative to the standard amount increased LEE and BC to 81.2 and 56.1%, respectively. Of the adsorbents evaluated, zeolite noticeably increased LEE to 98.7%. Increasing the amount of chloroform by 50% in the presence of zeolite caused a further significant increase in LEE and BC to 98.3 and 75.3%, respectively. These results indicated that biodiesel production from wet microalgae could be enhanced markedly by the addition of adsorbents with increased amounts of organic extraction solvents.
권민희,정상미,정봉환,김송근,변상직,라문진 강원대학교 농업생명과학연구원 2021 강원 농업생명환경연구 Vol.33 No.-
Among fatty acids, omega-3 and omega-6 fatty acids are called polyunsaturated fats because they have several double bonds. Both are biologically active and play an important role in immune system and metabolism processes in the body. The intake of omega-3 and omega-6 fatty acids in a ratio of 1:4–5, which is recommended by the World Health Organization for a balanced nutrition, can help prevent the onset of insulin resistance, depression, high blood pressure, stroke, and obesity. However, in modern diets, people consume too much omega-6 fatty acids. One reason is the consumption of mostly vegetable oils, which contain a high amount of omega-6 fatty acids. We hypothesized that a proper mix of oil extracted from pine nuts and general oils could provide the desirable functional benefits through ensuring a 1:4–5 ratio of omega-3 to omega-6 fatty acids. Therefore, this study investigated whether mixing pine nut oil with general oils could provide the ideal composition ratio of 1:4–5 of omega-3 to omega-6 fatty acids. Perilla, hazelnut, olive, avocado, and hemp seed oils were selected as the candidates for mixing with pine nut oil. The individual profiles of fatty acids and the omega-3:omega-6 fatty acids ratio in the mixtures were analyzed using a GC-FID system. Our results showed that the mixture of 85% pine nut oil and 15% perilla oil had an optimal omega-3 to omega-6 fatty acids ratio of 1:4–5. These findings suggest that the proportioned mixture of pine nut oil and perilla oil has the potential to develop oil blends with improved functionality.