Effects of Osmolality and Ca2+ on Sperm Motility in Marbled Sole, Limanda yokoha

고강희 釜山水産大學校 海洋産業開發硏究所 1998 硏究業績集 Vol.1997 No.-

Effect of Diluents on the Cold Storage of Sperm in Sea Urchin, Hemicentrotus pulcherrimus

고강희,강경호,김재민 한국발생생물학회 2003 발생과 생식 Vol.7 No.1

말똥성게 정자의 냉장보존을 위한 여러 가지 희석액의 보존 효과를 비교하였다. 다양한 농도의 포도당 용액, 인공해수(ASW)를 희석액으로 하여 정자를 4에서 보존하였다 보존효과는 정자 활성지수 (SAI), 생존율 및 말똥성 게 난에 대 한 수정률로 평가하였다. ASW와 1.2 M glucose액을 희석 액으로 하여 30일간 냉장보존하였을 때 가장 높은 운동성 및 생존율을 유지하였다. 정자의 냉장보존을 위한 희석액의 적정 pH는 7.0∼8.0 이었다. 정자 A series of experiments were conducted to compare the effects of various diluents in cold storage on the sea urchin, Hemicentrotus pulcherimus sperm. Various diluents of glucose solutions, artificial sea water(ASW) and 50% ASW were used to store the sperm at 4. The storage effect was evaluated using sperm activity index(SAI), survival rate of sperm and fertilization rate to egg. ASW and 1.2 M glucose were found to be better diluents which maintained high motility and survival rate of sperm f3r a storage period of 30 days. Optimal pH of diluent to store the sperm at 4 is 7.0∼8.0. In order to keep high SAI and survival rate of sperm, addition of 400 ppm neomycin into the diluent revealed the best storage results.

Effects of Osmolality and Ca²on Sperm Motility in Marbled Sole, Limanda yokoham

고강희 부경대학교 수산과학연구소 1997 硏究業績集 Vol.22 No.-

무지개송어(Oncorhyndhus mykiss)정자의 동결보존

고강희 전남대학교 수산과학연구소 2014 수산과학연구소논문집 Vol.22 No.-

This experiments was performed to identify the suitable cryoprotectants, freezing rate and, thawing rate for sperm cryopreservation in the rainbow trout, Oncorhynchus mykiss. In cry-opreservation experiments, 300 mM glucose solution containing 20 mM KCl, 20 mM Hepes and, pH 7.6 was used as a diluent, and 10% dimethyl sulfoxide (DMSO) was used as a cryoprotectant, and 10 % egg yolk was used as an addictive material. As a results of cry-opeservation, when 10 % DMSO was used as a cryoprotectant, sperm motility showed the highest rate at the height of 2 ㎝ away from the liquid nitrogen. Thawing at 10 ℃ for 30 seconds displayed the best result in the thawing rate.

용가자미 (Cleisthenes pinetorum herzensteini ) 정자의 동결보존

고강희 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.4

Mechanisms for the Initiation of Sperm Motility

고강희,강경호,장영진 한국발생생물학회 2003 발생과 생식 Vol.7 No.2

정자의 운동개시는 수정시에 정자와 난자가 만나기 위한 전제조건이다. 동물의 정자는 CAMP와Ca2'의 조절기구에 의해서 정자의 운동개시가 일어난다. 정자운동 활성 및 개시를 위한 세포 신호전달기구는 멍게류와 연어과 어류에서 많은 연구가 이루어져 왔다. 멍게류의 경우, 난에서 분비되는 정자 활성 및 유인물질(sperm-activating and -attracting factor)은 정자 활성 및 난으로의 유인을 위하여 외부의 을 요구한다. 한편 연어과 어 Initiation and activation of sperm motility are prerequisite processes for the contact and fusion of male and female gametes at fertilization. The phenomena are under the regulation of CAMP and in vertebrates and invertebrates. Mammalian sperm requires and cyclic AMP for the activation of sperm motility. Cell signaling for the initiation and activation of sperm motility has been well studied in the ascidians, Ciona intestinalis and C. savignyi and salmonid fishes. In Ciona, whose cell signaling for activation of sperm motility has been established, the sperm-activating and -attracting factor released from unfertilized egg requires extracellular for activating sperm motility and eliciting chemotactic behavior of the activated sperm toward the egg. On the other hand, the cyclic AMP-dependent phosphorylation of protein is essential for the initiation of sperm motility in salmonid fishes. A decrease in the environmental Ti concentration surrounding the spawned sperm causes a li efflux and influx through the specific channel and dihydropyridine-sensitive L-/T- type channel, respectively, thereby leading to the membrane hyperpolarization and influx. The membrane hyperpolarization synthesizes cyclic AMP, which triggers the luther Process of cell signaling, i.e., cyclic AMP-dependent protein phosphorylation, to initiate sperm motility in salmond fishes.almond fishes.

유령멍게 정소에서 스테로이드 호르몬 합성효소의 발현

고강희,장영진 한국수정란이식학회 2005 한국수정란이식학회 학술대회 Vol.2005 No.1

Identification and intracellular localization of carbonic anhydrase I in gills, heart, muscle, and intestine of rainbow trout, Oncorhynchus mykiss

고강희,김정우,김수철,최명락,한경호,이원교,최갑성 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.5

Carbonic anhydrases (CAs) play a key role in physiological functions such as pH homeostasis, calcification, photosynthesis, and ionic regulations. Among CA isozymes, CA I is a specific marker for the cytoplasm or apical cell membranes of colonic epithelial cells, and it is related to enterocyte proliferation in mammals. The major aim of the present study was to investigate the regional, cellular, and subcellular localization of CA I in a fish, rainbow trout, Oncorhynchus mykiss. The CA I was identified in the gills, heart, and intestine of the rainbow trout. CA I protein detected in the tissues of the rainbow trout was similar to a CA III with a molecular weight of 29 kDa. Immunohistochemical results demonstrated that CA I was localized in the various cells of gills, heart, muscle, and intestine of the rainbow trout.

유령멍게 정소에서의 스테로이드 호르몬 합성효소의 발현

고강희,장영진 한국발생생물학회 2005 한국발생생물학회 학술발표대회 Vol.2005 No.1

김치 종류에 따른 유산균의 생물학적 및 기능적 특성

고강희(Kang Hee Ko),유문려(Wenli Liu),이현희(Hyun Hee Lee),은걸(Jie Yin),김인철(In Cheol Kim) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.1

갓김치, 배추김치, 열무김치, 깍두기로부터 348종의 유산균을 분리하여 각 김치 종류에 따른 유산균의 특징을 확인하였다. 열무김치의 유산균은 다른 3종의 김치에 비해 간균+단간균:구균의 비가 5.6:1로 구균의 함량이 적었다. Leuconostoc속으로 추정되는 구균의 함량은 4종의 김치가 모두 유사하였으나 Lactobacillus 속으로 추정되는 간균과 단간균의 함량은 깍두기에서 60.7%로 높게 나타났다. 다른 김치에 비해 배추김치 유래 유산균 중 18.7%가 plasmid가 없었으나 plasmid를 지닌 유산균 중에는 열무김치 유래 유산균에 평균 4.1±0.5개의 plasmid bands가 나타났다. 세포 외 다당(EPS)을 5 mg/mL 이상 생산하는 유산균은 무를 주재료로 한 깍두기와 열무김치에 각각 11.1%, 10.9%로 갓김치와 배추김치보다 많았지만 배추김치 유래 유산균이 8.4±2.0 mg/mL의 EPS를 생산해 다른 김치 유래 유산균들보다 1 mg 이상 높았다. 갓김치에는 V. parahaemolyticus에 대한 항균력을 지닌 유산균이 많은 반면 열무김치, 배추김치, 깍두기에서 Bacillus 속, L. monocytogenes, Salmonella Typhimurium에 대해 항균력을 지닌 유산균이 갓김치보다 2배 이상으로 나타났다. 열무김치와 깍두기 유래 유산균 중 43.3%,45.5%가 내산성을 지녔으며, 특히 깍두기의 유산균 중 36.3%가 내담즙성을 나타내 다른 김치보다 많았다. Caco-2 세포에 대한 장내부착능을 지닌 유산균은 18.6%의 비율로 갓김치에 가장 많았다. 이러한 결과에서 볼 때, 김치에 함유된 유산균은 종이 한정적임에도 불구하고 김치 종류에 따라 각 김치에 함유된 유산균의 생물학적 특징에 차이가 있었으며, 특히 내산성, 내담즙성, 장내부착능을 지닌 유산균이 김치에 따라 차이가 나타남으로써 본 연구의 결과가 프로바이오틱 기능성을 지닌 유산균을 선별하는데 유용한 자료가 될 것으로 기대된다. Biological and functional characteristics of lactic acid bacteria (LAB) were investigated in mustard stem/leaf kimchi (MK), cabbage kimchi (CK), young radish kimchi (YRK), and cubed radish kimchi (CRK). LAB of young radish kimchi were mainly composed of bacilli in contrast to the other kimchi. 89.2% LAB isolated from all kimchi harbored plasmids. However, LAB had an average of 4.1±0.5 plasmid bands in YRK, more than MK, CK, and CRK. Exopolysaccharides were produced by 10.9~11.1% of LAB, and were especially by LAB isolated from radish kimchi. A significant percentage of LAB (69.5%) had antibacterial activity against one sensitive strain or more. LAB from CK, YRK and CRK had antimicrobial activities against Bacillus sp., Listeria monocytogenes, and Salmonella Typhimurium, while the LAB from MK had activities against Vibrio parahaemolyticus higher than those from the other kimchi. In YRK and CRK, acid-tolerant LAB were twice as prevalent as those in MK and CK. Bile-tolerant LAB isolated from CRK were more prevalent than other kimchi. When 10<SUP>8</SUP> CFU of LAB were added to Caco-2 cells, 12.1% of LAB isolated from all kimchi showed similar adherent activity to Lactobacillus rhamnosus GG. LAB of MK particularly adhered to Caco-2 cells, 2.0~4.1 fold higher than LAB in the other kimchi. From these results, biological and functional characteristics of LAB varied according to the type of kimchi and LAB existing in kimchi were limited to their respective species.