포도 Delaware의 無核果生産을 위한 Streptomycin利用 試驗(Ⅲ)

裵基煥,朴相眩,安相烈 진주산업대학교 1987 論文集 Vol.25 No.-

前 試驗에 이어 SM混用區의 GA_3濃度를 100PPM으로 높이고 處理時期의 폭을 滿開期前 20, 18, 16, 14, 12, 10, 8일로 하여 GA_3單用의 대체 可能性을 試驗한 結果는 다음과 같다. 1. 滿開期는 兩 主區 모두 無處理보다 2~3일 빨랐다. 2. 熟期는 兩 主區 모두 無處理보다 25일 정도 早熟되었다. 3. 無核化率은 混用區에서 56個區 중 2個區가 有核粒이 1~2개 나타났고, 單用區는 56個區 中 13個區에서 1~3個의 有核粒이 나타났다. 4. 收穫果房狀態는 兩 主區間에는 有意差가 인정되지 않았고, 各 細區間에는 果房長에서만 5%수준 以上의 有意差를 보여 處理時期가 빠를수록 길어졌다. The studies were conducted continuously following previous test to determine the possibility of uitilization with streptomycin for seedless berries production in Delaware grapes. The two main plots which were applied with different chemicals (combind solution of GA_3(100PPM)+SM(200PPM), GA_3(100PPM) alone solution) as pre-bloom application and the seven split plots according to days applied before full bloom were designed. The results obtained were as follows ; 1. The days of full bloom in both main plots was accelerated by two to three days compared with that in the untreatment. 2. The days of maturity in both main plots was accelerated by twenty-five days compared with that in the untreatment. 3. In combined solution plots, seed holding bearies were found in two out of fifty-six plots, but in alone solution plots they were found in thirten out of fifty-six plots. 4. The status of cluster harvested in both main plots were nonsignificant, but the lenght of cluster havested in each split plot were singificant at the 5 percent level.

棉實의 筋收縮, 血壓降下 및 過血糖作用

배기환 충남대학교 자연과학연구소 1984 忠南科學硏究誌 Vol.11 No.1

The purpose of this study was to investigate the action of water extract of Gossypium Semen(GWE). It was clarified that GWE caused the contraction of an isolated frog's rectus abdominis muscle, the fall of the blood pressure in a rabbit, and the hyperglycemia in a hen.

은행잎 플라보놀배당체에 대한 셀루라제류의 영향

배기환,민병선,백흠영,안병준 충남대학교 암연구소 1991 癌共同硏究所 硏究誌 Vol.1 No.1

The extractability and stability of ginkgoflavonolglycosides under presence of several cellulase preparations were investigated. The enzymes used were macerosin, cellulase C and cellulase NC. The content variation of the glycosides was measured with HPLC method, using caffeic acid as an internal standard. The methanol extract of ginkgo leaf, containing the total flavonolglyco-sides of 4.46%, was used for the content comparison. By extraction with the enzymes, each or mixed, the peak levels of all the glycosides began to decrease after 1 or 2 hours. After 24 hour extraction, most of the glycosides were degraded to minor components. The flavonolglycosides in ginkgo leaf were also hydrolysed simply by the water extraction. After 24 hour extraction with water at 40°C, the peak levels of major glycosides were distinctly decreased. Rutin was hydrolysed by enzyme treatment or by ginkgo leaf itself. As a result, it was concluded that the commercially available cellulases and the ginkgo leaf itself contain the activities of β-glycosidase and α-rhamnosi-dase. Kaempferol-3-0-(6' -0-p-coumaroylglucosyl)-rhamnoside and four other ginkgo flavonolglycosides were not hydrolysed under the same condition."

포도 Muscat Bailey A의 安定生産을 위한 Gibberellin 處理試驗 (1)

裵基煥 진주산업대학교 1980 論文集 Vol.18 No.-

포도 Muscat Bailey A는 品種의 生産安定을 期하기 위하여, 이 地方에 알맞는 Gibberellin의 單回處理의 適期를 모색코저 100ppm. 濃度로써 滿開 直後(G_1區)와 滿開後 4日(G_2區), 7日(G^3區), 10日(G_4區), 13日의 處理區(G_5區)를 두어 그 處理가 果房에 미치는 影響을 調査하였던 바 다음과 같은 結果를 얻었다. 1. 熱期는 處理區가 無處理區에 비하여 1∼2日 程度 이르고, G_1區는 다른 處理區에 비하여 1日 정도 遲延되는 傾向이 있었다. 2. 果房長은 無處理區에 비하여 G_1, G_2區만이 길고, 其他의 處理區는 有意差가 없었다. 3. 果房重은 處理區가 모두 無處理區에 비하여 무겁고, 各 處理區間에는 有意差가 없었다. 4. 着粒數는 無處理區에 비하여 G_1區만이 많고, 其他의 處理區는 有意差가 없었다. 5. 平均果粒重은 G_1區를 除外하고는 各 處理區가 모두 無處理區에 비하여 무거웠고, 이들 各 處理區間에는 有意差가 없었다. 6. 果房內 4g 未滿의 小粒含有率은 無處理區에 비하여 G_1區는 현저히 높으나, 반대로 G^3, G_4, G_5區는 현저히 낮으며, G_2區는 有意差가 없었다. 7. 以上의 今年度의 試驗結果로 미루어서는 本品種에 대한 處理適期는 滿開後 7日부터 13日 사이에 있는 것으로 思料된다. In order to find the best time of gibberellin application for safe production of Muscat Bailey A grapes, this study was designed. GA_3 at 100 ppm concentration was applied by dipping the clusters in accordance with the designed treatments. The designed treatment were the following 5 kinds :G_1 treatment applied on the first day, G_2 treatment on the 4th day, G_3 treatment on the 7th day, G_4 treatment on the 10th day, G_5 treatment on the 13th day after full bloom. The results were as follows: 1. The maturities in all treatments were forced by one or two days compared with that in untreatment. 2. The cluster's lengths in G_1 and G_2 treatments were long, but those in the otder treatments were not different significantly, compared with that in untreatment. 3. The cluster's weights in all treatments were heavy compared with that in untreatment, but significant differences were not shown between those in these treatments. 4. The number of berries per cluster in G_1 treatment was largy, but those in the other treatments were not different significantly, compared with that in untreatment. 5. The mean weights of berry in the other treatmendts except G_1 treatment were heavy compared with that in untreatment, but significant differences were not shown between those in those treatments. 6. The percentage of light berries not exceeding 4gr. in G_1 treatment was remarkbly high, but on the contrary those in G_3, G_4 and G_5 treatments were remarkbly low, and that in G_2 treatment was not different significantly, compared with that in untreatment. 7. From the results of this study,I judged that the best time for gibberellin application was from the 7th day to 13th day after full bloom.

7,8-Dioxo-A-norerythrinan의 합성

배기환,서원준 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

7,8-Dioxo-A-norerythrinan (A) was synthesized from acid catalyzed cyclization of 6a-hydroxy-1-(2-phenylethyl)-octahydrocyclo-penta[b]pyrrole-3a-caboxylic acid ethyl ester (B) with concomitant deethoxycarbonylation. The intermediate (B) was a hydroxylated compound of N-acyliminium (C). The unstable pyrrolinium (C) was prepared from oxalylation of the enamine of phenethylamine and ethyl 2-oxocyclopentanecarboxylate.

L1210 세포에 대한 약용 식물의 세포독성 검색

배기환,민병선,도동선,김남수,양기종,안병준 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

For the research of cytotoxic natural products, 50 medicinal plants were extracted with benzene and methanol, separately, and screened against L1210 cells. From the results(Table I), 6 samples showed cytotoxicity both in benzene and methanol extracts of 17 samples in benzene extracts and 3 samples in methanol extracts, respectively. Generally, the cytotoxicity exhibited high frequency (34%) in benzene extract but low frequency in methanol extract(6%), it meant that active cytotoxic components had less polarity. ED_50 values less than 10 ㎍/㎖ were observed in 17 medicinal plants.

대추로부터 베튜리닉 산과 알피톨릭 산의 분리 및 정량

배기환,이상명,이은실,이준성,강종성 충남대학교 약학대학 의약품개발연구소 1996 藥學論文集 Vol.12 No.-

Betulinic acid and alphitolic acid, the triterpenoids of Zyziphi Fructus, were isolated with silica gel column chromatography and used as the standard substances for the analysis. The compounds were determined with HPLC and HPTLC. They were separated on reversed phase coumn (Nova-Pak C_18) with 0.05 M Na_2HPO_4-methanol (19:81) in HPLC and detected at 210 ㎚. Separation on HPTLC precoated sillica gel F_254 plates was carried out with chloroform-methanol (6:1) and the separated compounds were reacted with ρ-anisaldehyde and detected at 540 ㎚. The contents of betulinic acid and alphitolic acid in Zyziphi Fructus from four different regions in Korea were in the range of 2.9~3.8% and 3.2~3.9%, respectively.

4,4'-Biphenol 유도체의 합성 및 충치균 Streptococcus mutans OMZ 176에 대한 항균작용

배기환,서원준,임승희 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

In a continuous study for the developing of the anticariogenic agents, 3,3'-diacyl-4,4'-biphenol derivatives (Fig.2, 2 and 3) and 3,3'-bis-(l-hydroxyalkyl)-4,4'-biphenol (Fig.3, 4 and 5) derivatives are synthesized successively from 4,4'-diphenol (Fig.2, l). The synthesized compounds are tested for their antibacterial activity against a cariogenic bacterium, Streptococcus mutans OMZ 176. The acyl derivatives, 2 and 3, do not show antibacterial activity, but the hydroxyalkyl derivatives, 4 and 5, reduced from the acyl group of 2 and 3, show the activity. The antibacterial activity of 2 and 3 may be inhibited due to intramolecular hydrogen bonding between the acyl group and the hydroxyl one (Fig.4).

포도 Muscat Bailey A의 無核果生産을 위한 Gibberellin 의 處理試驗(1)

裵基煥 진주산업대학교 1983 論文集 Vol.21 No.-

포도 Muscat Bailey A 品種의 無核果生産을 위한 Gibberellin의 前處理 適期를 모색코저 本 試驗이 試圖되었다. 生育이 良好한 17年樹 供試하여 後處理는 滿開 後 8日에 모든 處理區를 同一히 하고, 前處理時期는 滿開 前 14日(G_1區), 12日(G_2區), 10日(G_3區), 8日의 處理區(G_4區)를 두어, 前後 處理 共히 GA_3 100 ppm 濃度로서 果房을 浸漬處理하여 그 處理가 果房에 미치는 影響을 調査하였던 바, 다음과 같은 結果를 얻었다. 1. 熟期는 모든 處理區가 無處理區에 비하여 約 20日 旱熟되었다. 2. 無核粒의 比率은 處理區가 無處理區에 비하여 有意的으로 높으나, 處理區라 할지라도 有核粒의 含有率이 높아, 無核果生産을 위한 滿足한 結果를 얻지 못하였다. 3. 果房長은 G_4 區를 除外한 그외의 處理區는 無處理區에 비하여 有意的으로 길었다. 4. 果房重은 無處理區에 비하여 G_2 및 G_3 區만이 有意的으로 무거웠고, G_1 및 G_4區는 有意差가 없었다. 5. 着粒數는 處理區라 할지라도 無處理區에 비하여 有意性이 없었다. 6. 平均果粒重은 處理區가 無處理區에 비하여 有意的으로 무거웠다. 7. 果粒의 均一度는 處理로 因한 有意性이 없었다. 8. 果粒의 密着度는 無處理區에 비하여 G_3 및 G_4區는 有意的으로 密着되나, G_1 및 G_2區는 有意性이 없었다. In order to find the best time of gibberellin application for seedless berry production in Muscat Bailey A grapes, this study was designed. The 17­year­old trees grafted on Teleki No. 8B rootstocks were selected as test trees. GA_3 at 100 ppm concentration was applied twice before and after full bloom by dipping the clusters. The before applications were carried out on different days according to designed treatments, and all the after application on the 8th day after the full bloom. The designed treatments were the the following 4 kinds : G_1 treatment applied on the 14th day, G_2 treatment on the 12th day, G_3 treatment on the 10th day, G_4 treatment on the 8th day before the full bloom.The results obtained through all the treatments were not satisfiable for seedless berry production, but the maturity in all the treatments were hastened by about 20 days compared with in the untreatment. Therefore, these treatments showed the possibility to early mature cultivation. The results of this study suggested that the practical before application period for early mature cultivation was within the range of the 12th day to the 10th day before full bloom.

傾斜地 果樹園의 開園方法과 土壤管理法이 土壤의 粒團化와 腐埴含量에 미치는 影響

裵基煥 진주산업대학교 1970 論文集 Vol.6 No.-

傾斜地 果樹園에 알맞은 合理的인 開園方法과 土壤管理法을 摸索하기 위하여, 이와같은 곳에 알맞으리라 假想되는 몇가지의 開園方法과 土壤管理法을 選定하여, 이들이 果樹園의 地力과 가장 關係가 깊은 土壤의 粒團形成과 腐埴含有量에 미치는 影響을 調査하였다. Split plot design에 依據하여, 主區로서는 等高線難立區와 階段田構築區 및 標準區를 두고, 細區로서는 淸耕區와 草生區, 敷草區의 3種類를 두어 試驗하였는데, 試驗結果의 大要는 다음과 같았다. 1. 各 試驗區의 粒團分析 結果는 開園方法間에서는 階段田構築區가 粒團含量이 越等히 높고, 等高線難立區도 標準區에 比하여 높은 傾向이 있었으며, 土壤管理法間에서는 敷草區가 다른 區보다 높은 傾向이 있었다. 2. 本 試驗地의 腐埴含有量은 平均 1.85%에 不過하였으며, 年中의 腐埴消耗量은 全腐埴量의 15%에 達하는 아주 높은 消耗量을 나타내고 있었다. 3. 試驗區에 따른 腐埴含有量은 開園方法間에서는 階段田構築區와 等高線難立區가 標準區에 比하여 越等히 높았고, 土壤管理法間에서는 草生區-敷草區-淸耕區의 順位로 높았다. As a trial to find an effective establishment method and soil management system for slope-land orchard, this study was made to investigate effects of those on granule formation and humas quantity in orchard soil. The author established three main plots-control, contour tillage and bench terrace plot-and three split plots-clean culture, sod culture and grass mulch plot-according to split plot design. Sample soil of each experimental plot was gathered on November 2 this year and analysed. The results obtained as follows: 1. Granule formation among establishment methods was most excellent in bench terrace plot soil, also one in contour tillage plot soil was considerably good compared with that in control plot soil. Among soil management systems, one in grass mulch plot soil tended to be good than those in other plot soil. 2. The slope land used as experimental plots had humas quantity of only 1.85 percent, and this land exhausted a largy humas quantity amounted to 15 percent of total humas in a year. 3. Humas quantities in both bench terrace and contour tillage plot soil were much more compared with that in control plot soil. Among soil management systems, one in sod culture plot soil was most and one in grass mulch plot soil more than that in clean culture soil