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金龍昊,蔡永岩 서울大學校 農科大學 1985 서울대농학연구지 Vol.10 No.1
本 實驗은 callus水準에서 담배 역병 저항성 세포주의 선발 가능성을 검토하기 위하여 실험을 수행하였으며 얻어진 결과는 다음과 같이 요약할 수 있다. 1. 담배 심부 절편을 배양하여 callus를 유기시키는데는 IAA 2mg/l+kinetin 0.2mg/l가 添加된 Murashige-Skoog(MS) 배지가 가장 良好하였다. 2. Callus에 接種原을 接種시킬 때 反應을 잘 觀察할 수 있는 接種原의 濃度는 callus當 3個정도의 유주자낭(유주자 30∼60개)이 가장 適當했다. 3. 接種後 callus의 反應에는 호르몬의 濃度가 影響을 미쳤는데 IAA 2mg/l+kinetin 0.2mg/l은 低抗性을 IAA 2mg/l+kinetin 0.2mg/l은 罹病性을 많이 發現시켰다. 4. 低抗性 反應을 보인 callus를 繼代培養하여 再接種한 結果 低抗性 程度는 처음 반응과 같았다. 5. Callus에서 shoot의 分化는 BA 2mg/l를 첨가한 MS배지가 가장 좋았으며, 뿌리의 分化는 호르몬이 添加되지 않은 MS배지가 가장 良好했다. 6. 低抗性 callus에서 分化시킨 植物體를 土壤接種했을 때의 反應은 callus에서의 反應과 같아 callus水準에서 선발한 低抗性은 再分化 植物體에서도 유지됨을 알 수 있어 callus水準에서의 耐病性 細胞株의 選拔 可能性이 입증되었다. This experiment was carried out to know the possibility for the selection of resistant cell line at callus level. Phytophthora parasitica var. nicotianae was used as inoculum. The results are summarized as follows; 1. Addition of 2mg/l IAA and 0.2mg/l kinetin to Murashige-Skoog basal medium was the best hormonal combination for callus induction in tobacco pith culture. 2. Three zoosporangia per callus piece was optimum level to distinguish the response of calli. 3. The effect of differing cytokinin and auxin concentration on the resistance of tobacoo tissue culture were important. "Hypersensitive reaction" was observed when adding IAA 2mg/l and 0.2mg/l kinetin, however, hypersensitive reaction did not occur in case of IAA 2mg/l and 2mg/l kinetin addition to the medium. 4. Resistance was maintained in subculture of resistant calli. 5. Shoots were developed when adding BA 2mg/l to Murashige-Skoog medium, and roots were differentiated in free hormonal Murashige-Skoog medium. 6. The results of seeding test was so well corresponded with that of callus lines could be selected as breeding materials based on callus reaction to the pathogen.
국내 자생 배향초의 화학형 분류와 수집지역에 따른 화학형의 분포
채영암 서울대학교 농업개발연구소 1999 농업생명과학연구 Vol.3 No.-
Equilibrium headspace analysis connected with gas chromatography/mass spectrometry was used to identify volatile compounds released from the leaves of 73 individual plants of Agastache rugosa collected from 11 sub-regions in South Korea. Chemometric investigation of the infra-specific variability in volatiles led to identify five main chemotypes; elaborated estragole (Type 1) as the major component, and the other four chemotypes, characteristically contain monoterpenois compounds, isomenthone (Type 2), pulegone (Type 3), limonene (Type 4) and isomenthone+pulegone (Type 5), In these chemotypes, pulegone, limonene and isomenthone+pulegone types revealed that the diversity of new chemotypes were present in Korea.
崔奎煥,蔡永岩 서울大學校 農科大學 1988 서울대농학연구지 Vol.13 No.1
This experiment was carried out to determine the proper condition for protoplast isolation and fusion in Nicotiana sylvestris and N. plumbaginifolia, and plant regeneration from fused cells. Enzyme mix of 2.0% cellulase R10 0.5% macerozyme R10 was proper for protoplast isolation from leaf mesophyll cells. Optimum fusin frequency was observed in solution(pH5.8) of PEG(6000) 35% and 9mM CaCl₂Callus was induced 20 days after plating on culture medium. Shoots were formed 6 weeks after plating on shooting medium and rooted on MS basal medium thereafter. Three out of 20 regenerated plants were identfied as somatic hybrids by peroxidase isozyme analysis.
채영암,류수노,이봉호,김관수,방진기,송지숙 한국국제농업개발학회 1999 韓國國際農業開發學會誌 Vol.11 No.1
Changing lifestyles in developed countries have led to dramatic increases in demand for natural products, particularly over the last decade. More varied use has been made of oils and herbs in cooking, and oil components are now being regarded as safe alternatives to synthetic food additives and crop protection substances. Volatile compounds among the secondary metabolites in plants have a great potential for their industrial application. With the development of advanced analytical technologies, various studies have been conducted on chemical properties, biosynthetic pathways, and functional bioactivity of the essential oil compounds in plants. Genetic studies, however, for these compounds have not been actively conducted, which is mainly due to difficult methodologies and their high cost of analytical instruments. One of the most difficult procedures for analysis of volatile compounds is the step of their extraction from plants. A few methodologies of extraction and trapping for these compounds have been introduced in terms of different experimental purposes. Recently, either equilibrium headspace autosampler or solid-phase microextraction(SPME) coupled with gas chromatography-mass selective detection was introduced to be a rapid and reliable method for qualitative evaluation of volatile compounds including terpenes and phenylpropenes in the aromatic plant germplasms and comparison between natural and synthetic compounds. In this study, we described the chemical properties of volatile compounds extracted from plant resource and introduced the various analytical techniques for their isolation and identification. We would discuss later the possibility of these analytical technologies for application to quality evaluation of volatile oil compounds and cultivar development.