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      • SCIEKCI등재

        Cyclodextrin 분해효소의 정제 및 그 특성

        문영희,김용휘,심규광 한국농화학회 1990 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.1

        Cyclodextrin hydrolase from Bacallus stearodhermophilus KFCC 21203 was purified and the properties of the purified enzyme were investigated. The enzyme was purified 15 folds with 77% recovery by ammonium sulfate fractionation, DEAE-cellulose chromatography, and Ultro AcA 34 gel filtration. The specific activity and the molecular weight of the enzyme were 1.30 units/㎎ protein and about 29,500, respectively. The maximum activity of the enzyme was shown at 55℃ and pH 5.5. However, stable temperature and pH were 40 ℃ and 5.0∼8.0, respectively. The Km value for γ-cyclodextrin was 3.78 × 10^(-3) M. The degradation activity of the enzyme was selectively high for γ-cyclodextrin, and very low for β-cyclodextrin, but not for α-cyclodextrin. The decomposed products of γ-cyclodextrin were mainly glucose and maltose, and a little mlatotriose. The activity of the enzyme was very high for amylose, potato starch, corn starch, amylopectin and maltooligomer, and relatively high for glycogen and dextrin. The decomposed products of them were mainly glucose and maltose.

      • 생강나무의 消炎. 鎭痛作用에 관한 연구(Ⅱ)

        文永熙,高在鍾,金庚完 朝鮮大學校 1996 藥學硏究誌 Vol.18 No.1

        Lindera obtusiloba has been used for the treatment of fever, abdominal distention and pain in traditional medicine. The anti-inflammatory activity of the extracts from Lindera obtusiloba was investigated utilizing carrageenin-induced edema, granuloma pouch in rats. The effects of this agent on capillary vascular permeability and acetic acid-induced writhing in mice were also examined. It showed to have significant analgesic activity on the writhing syndrome in mice induced by 0.7% acetic acid at Water extract of root(600㎎/㎏). It was also inhibites the increase in carpillary vascular permeability induced by acetic acid in mice. It showed anti-inflammatory activity in 1% carrageenin-induced edema with oral administeration in rats and exhibited significant preventive effect on edema at Water extract of root(600㎎/㎏). The water extract of root from Lindera obtusiloba showed significant inhibition on granuloma and exudate formation in rats. In the present study, the Water extract of root was indicated to have the analgesic and anti-inflammatory activity.

      • 白毛藤엑기스가 白鼠의 實驗的 肝臟障碍에 미치는 影響

        文永熙,姜壽鐵 조선대학교 약학연구소 1989 藥學硏究誌 Vol.11 No.1

        In order to study the effect of extracts of Solanum lyratum Thunberg on the liver damage of the rats intoxicated with CCl^4, the activities of GPT, GOT, Al.P, LDH and the levels of total cholesterol, total bilirubin in serum were measured. The results were as follow: The extracts (M-Ex, W-Ex.) wee showed to be significanfty decrease in the activities of GPT and GOT in serum rats intoxicated by CCl_4. The Al.p activity was significantly decreased on the 2nd day after administration of the extracts. The LDH acticity was significantly decreased in all sample groups, but W-Ex was more decreased than M-Ex. The level of total cholesterol and total bilirubin was showed to be signicantly decreased on the 2nd and 4th days after administration of M-Ex., but W-Ex. was not influenced, when copared with control group. Acording to the above results, it is thought that the extract of Solanum lyratum improves the hepatic function in the CCl_4-infoxicated rats.

      • 植防風 Extract의 家兎血壓에 미치는 影響

        文永熙,白采善,高錫太 조선대학교 약학연구소 1983 藥學硏究誌 Vol.5 No.1

        Intravenous injection of Peucedani Japonici Radix Extract (PJE) regularly elicited the dose-related lowering of blood pressure in the rabbits anesthetized with urethane, and then did not show the cumulative effect and the tachyphylaxis. The hypotensive action induced by PJE was inhibited by pretreatment with atropine or bethanidine, but potentiated slightly by preteatment with physostigmine. The hypotensive action induced by PJE was not influenced by propranolol, phentolamine, chlorisondamine or diphenhydramine. Above results suggest that the PJE produces the hypoensive action by dual action, that are muscarinic action and inhibition of adrenergic activation.

      • KCI등재

        전북지역 양호교사의 AIDS 관련 지식과 태도 조사연구

        정영숙,문영희 韓國保健敎育學會 1994 보건교육건강증진학회지 Vol.11 No.2

        AIDS preventional efforts need to be guided by well trained health care personnel especially by school nurses for the adolescents. This study was designed to get basic data about AIDS educational program development for school nurses. The objectives of this study were to 1) identify AIDS knowledg of school nurses 2) identify AIDS attitudes of school nurses and 3) identify association between AIDS knowledge and sociodemographic characteristics. Data were collected from 173 subjects in Chonbuk province. Self-reporting questionnaire were administered during the period from 1st of June to 30th of June, 1994. AIDS related knowledge was measured by using 44 questions on cause(3 items), testing(3 items), mode of transmission(15 items), clinical manifestations(5 items), treatment(3 items), prevention(5 items), complication(4 items), infection control(3 items) and Using resources(3 items). AIDS related attitudes were measured by five point Likert scales using 13 questions on perceived threats from AIDS crisis(4 items), perceived severity to AIDS(2 items), perceived needs about psychosocial care for HIV infected patients(3 items) and perceived educational needs of AIDS(4 items). The collected data were analyzed by SPSS/PC+, using percentages, Mean and S.D. descriptive purpose and t-test or F for comparing the variables. The major findings were as follows: 1. Respondents ranged in knowledge of AIDS between 0 and 44 with the 33.79 mean score. Percentage of correctly answered respondents to each categories -mode of transmission :87.0% -clinical manifestation :85.0% -cause :82.5% -prevention :81.5% -treatment :76.1% -infection control :72.8% -testing of HIV infection 71.8% -using community resourses :50.9% -complication :45.8% 2. The respondents' attitudes of AIDS ① The majority(70%) showed higher perceived threat ② The majority(91.3%) showed higher perceived severity to AIDS crisis ③ As a whole, perceived needs about psychosocial care for HIV infected patients negative ④ The majority(over 96.5%) showed highly perceived educational needs of AIDS. 3. The relationship between AIDS knowledge and each of those general character. ① AIDS knowledge shows significant difference with age(F=3.50, p<.016), years of professional experience(F=4.14, p<.007) and received lecture about AIDS(F=4.54, p<.000). ② There was no significant difference between AIDS knowledge and job satisfaction.

      • KCI등재
      • KCI등재

        중국 2016년 개정 「조선말 띄여쓰기」의 변화 내용과 남북한 현행 띄어쓰기 규정 비교

        문영희,조일영 민족어문학회 2017 어문논집 Vol.- No.81

        The prupose of this study is to compare between newly revised contents of Joseonmal Ttuieosseugi Gyujeong (Word Spacing Rules for Korean Language) in ‘4 general provisions’ in Chinese Joseonmal Gyubeomjip (Collection of Standards for Korean Language) (2016) and the Korean Spacing Word Rules of current South & North Korea. Before the division of Korea, the Korean people maintained their language life based on Hangeul Orthography Unity (1933, HOU). After the liberate from the Japanese control, however, South Korean, North Korean, and China’s Joseonmal have different language standard with different social system. After liberation of China, China’s Joseonmal followed Word Spacing rules in Hangeul Orthography Unity (1946, HOU), Word Spacing rules in Joseoneo Cheoljabeop (Spelling Rules for Korean Language) (1954) of North Korea, and Joseonmal Gyubeomjip (Collection of Standards for Korean Language) (1966) of North Korea. In 1977, Joseonmal Ttuieosseugi (Word Spacing Rules for Korean Language in china) was enacted in the way of corresponding with Joseonmal Gyubeomjip (Collection of Standards for Korean Language) of North Korea. In 1995, 2004, and 2007, Joseonmal Ttuieosseugi (Word Spacing Rules for Korean Language) was revised and applied in china. China’s Joseonmal spacing word regulation needed adjustment because of the morphologic features of China’s Joseonmal, but it had not had scientific characteristic enough because of the effect of custom and economic feasibility. Regarding these problems, new Joseonmal Gyubeomjip (Collection of Standards for Korean Language in china) was published in 2016. This study analyzes and compares Joseonmal Ttuieosseugi (Word Spacing Rules for Korean Language), which are revised the most on this revision, to Korean spacing word rules of current South & North Korea. This study has the significance for noticing the normative differences between Korean and China’s Joseonmal to high level korean learners through comparing the China’s Joseonmal standard to that of South & North Korean. Moreover, the direction of progressive standardization of Korean language can be assumed with this study, since the normative changes of China’s Joseonmal could be regarded as process of completion of unified Korean language standard. 이 연구는 새로 개정된 중국 『조선말규범집』(2016) ‘4칙’ 중의 「조선말 띄여쓰기」 규정의 개정 내용을 살펴보고 현행 남북한의 띄어쓰기 규정과 비교하는 데 목적이 있다. 우리 한민족은 남북한 분단 이전에는 모두 같은 『한글 마춤법 통일안』(1933)에 근거해 언어생활을 영위하였지만 광복 이후에는 남한, 북한과 중국 조선족이 각각 다른 사회 체제 속에서 사용하는 어문 규범 또한 달라지게 되었다. 중국에서 조선어는 해방 후에 『한글맞춤법통일안』(1946)의 「띄어쓰기」 규정과 북한의 『조선어철자법』(1954) 중의 「띄여쓰기」 규정 그리고 북한의 『조선말규범집』(1966)에 준하여 사용하다가 1977년에 북한의 『조선말규범집』과 일치시키는 방향으로 「조선말띄여쓰기」를 제정하였고 1995년과 2004년, 2007년에 「조선말띄여쓰기」를 일부 개정하였다. 띄어쓰기에 관해서 조선어의 형태론적 특성을 반영하는 적당한 수정이 있어야 했지만 관습의 영향과 경제성 등으로 인하여 과학성이 충분히 보장되지 못한 상태가 계속 유지되고 있었다. 이런 문제점들을 고려하여 2016년에 새로운 규범집이 출판되었다. 본고는 이번 개정에서 변화가 제일 큰 「조선말 띄여쓰기」 규정의 바뀐 부분을 중심으로 살펴보고 이에 해당하는 남북한 현행 띄어쓰기 규정과 비교하였다. 이 연구는 중국 조선족들이 사용하고 있는 어문 규범을 남북한 어문 규범과 비교하면서 중국인 한국어 고급 학습자들에게 한국어와 중국 조선어의 규범상의 차이를 보이는 데도 의의가 있지만, 이를 통해 한국어 어문규범의 앞으로의 진행 방향을 가늠해 보고자 함에도 의미를 둔다. 중국 조선어문 규범상의 변화가 통일한국어문규범을 완성해 가는 한 과정으로 이해할 수도 있기 때문이다.

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