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강병화,Kang, Byeung-Hoa 한국잡초학회 1984 Weed&Turfgrass Science Vol.4 No.2
Chlorsulfuron (chemical name : 2-Chloro-N-(4-methoxy-6-methyl-1,3,5-triazin-2-yl)-aminocarbonyl-benzenesulfonamide) is a herbicidally active ingredient which shows effect against susceptible weeds already at such low rates like 5-20g active ingredient per hectare. In the here reported trials metabolism in several sensitive cultivated plants and weeds have been analysed using ^{14}C-labelled active ingredient. The uptake of chlorsulfuron by leaves or the root system is good in all plants species, and translocation takes place either symplasmatically or apoplasmatically. Metabolism takes place in all investigated plant species by development of hydrophile suhstances in roots and shoots. Decomposition of chlorsulfuron in roots and shoots of tolerant species (Triticum aestivum and Hordeum vulgare) to polare substances takes place quantitatively faster and quicker than in susceptible species (Beta vulgaris and Matricaria chamomilla).
임균의 R-Plasmid에 의해 생산되는 Beta-Lactamase의 정제에 관한 연구
박정희,구자현,이성록,고재경,오영배,강병화 건국대학교 의과학연구소 1991 건국의과학학술지 Vol.1 No.-
The beta-lactamase was easily and effectively prepared from E. coli transformant having bla gene cloned from R-plasmid of N. gororrhoeas. Through osmotic shock treatment, DEAE-celluse column chromatography, Sephadex G-100 column chromatography and high performance liquid chromatography-gel filtration using Protein column I-125 the beta-lactamase was purified 52 fold. The purified enzyme prepared gave a single protein band on SDS polyacrylamide gel electrophoresis. From the results of these experiments, it was realized that the beta-lactamase produced in this transformant strains was composed of single subunit protein and was localized mostly in periplasm.