http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
배 검은별무늬병(Venturia nashicola) 고도 저항성 ‘93-3-98’ 유래 PR-10 유전자의 특성
천재안,김세희,조강희,김대현,최인명,신일섭 한국식물생명공학회 2015 식물생명공학회지 Vol.42 No.1
A PyrcpPR-10 gene with differentially expressedwas isolated by using the suppression subtractive hybridizationassay between ‘93-3-98’ (highly resistant against scab causedby Venturia nashicola) and ‘Sweat Skin’(highly susceptible)and analyzed the expression pattern according to organs andcultivars. The full length of PyrcpPR-10 was cloned as743bp with 480bp’s ORP, and was determined to encode aprotein of 159 amino acid residues. On analyzing PyrcpPR-10gene sequence compared with resistant and susceptiblecultivars, ‘Hwangsilri’ (resistant), ‘Gamcheonbae’ (moderatelyresistant), ‘Wonhwang’ (moderately susceptible), ‘Niitaka’(highly susceptible), and ‘Sweat Skin’ (highly susceptible)had identical gene sequence but ‘Bartlett’ (highly resistant)showed partly different sequences. The deduced amino acidsequence showed 64 ~ 98% homology and had the GXGGXGmotif to known amino acid of other plants PR-10 by theBLAST X analysis. Among several organs or tissues, petalwas showed highest expression level of PyrcpPR-10 genefollowed by leaf, floral axis, bud, and bark. The expressionlevel of PyrcpPR-10 gene was dramatically increased at 24hr after inoculation in all cultivars and also up-regulated inaccordance with resistant degree of cultivars. While resistant cultivars (‘Bartlett’, ‘93-3-98’, and ‘Hwangsilri’) inducedrelatively high expression level of PyrcpPR-10 gene, susceptiblecultivars (‘Niitaka’, and ‘Sweat Skin’) showed lowexpression level. PyrcpPR-10 gene is assumed that it isdirectly connected with defense mechanisms to pear scab.