Treadmill exercise ameliorates disturbance of spatial learning ability in scopolamine- induced amnesia rats

Yu-Mi Heo,Mal-Soon Shin,Su-Hyun Kim,Tae-Wook Kim,Sang-Bin Baek,Seung-Soo Baek 한국운동재활학회 2014 JER Vol.10 No.3

Quality Characteristics of Mayonnaise Containing Orange Peel

Yu Mi Heo,Soon Sil Chun,Seong Hee Choi,Jeong Ro Park 한국식품영양학회 2010 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2010 No.06

The expression of the receptor for advanced glycation end-products (RAGE) in RA-FLS is induced by IL-17 via Act-1

Heo, Yu-Jung,Oh, Hye-Jwa,Jung, Young Ok,Cho, Mi-La,Lee, Seon-Yeong,Yu, Jun-Geol,Park, Mi-Kyung,Kim, Hae-Rim,Lee, Sang-Heon,Park, Sung-Hwan,Kim, Ho-Youn BioMed Central 2011 ARTHRITIS RESEARCH AND THERAPY Vol.13 No.4

<P><B>Introduction</B></P><P>The receptor for advanced glycation end-products (RAGE) has been implicated in the pathogenesis of arthritis. We conducted this study to determine the effect of interleukin (IL)-17 on the expression and production of RAGE in fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). The role of nuclear factor-κB (NF-κB) activator 1 (Act1) in IL-17-induced RAGE expression in RA-FLS was also evaluated.</P><P><B>Methods</B></P><P>RAGE expression in synovial tissues was assessed by immunohistochemical staining. RAGE mRNA production was determined by real-time polymerase chain reaction. Act-1 short hairpin RNA (shRNA) was produced and treated to evaluate the role of Act-1 on RAGE production.</P><P><B>Results</B></P><P>RAGE, IL-17, and Act-1 expression increased in RA synovium compared to osteoarthritis synovium. RAGE expression and production increased by IL-17 and IL-1β (*<I>P <</I>0.05 vs. untreated cells) treatment but not by tumor necrosis factor (TNF)-α in RA-FLS. The combined stimuli of both IL-17 and IL-1β significantly increased RAGE production compared to a single stimulus with IL-17 or IL-1β alone (<I>P <</I>0.05 vs. 10 ng/ml IL-17). Act-1 shRNA added to the RA-FLS culture supernatant completely suppressed the enhanced production of RAGE induced by IL-17.</P><P><B>Conclusions</B></P><P>RAGE was overexpressed in RA synovial tissues, and RAGE production was stimulated by IL-17 and IL-1β. Act-1 contributed to the stimulatory effect of IL-17 on RAGE production, suggesting a possible inhibitory target for RA treatment.</P>

서울지역 공업계 특성화 고등학교 실험 · 실습실의 유해환경에 대한 전문교과 교사의 인식

허유미(Yu-Mi Heo),유현석(hyun-seok Yoo) 대한공업교육학회 2020 대한공업교육학회지 Vol.45 No.3

특성화고의 전문교과 교사들은 업무시간과 이론 중심 과목의 수업을 제외하고는 대부분 실험 · 실습실에서 수업을 진행하기 때문에 장시간에 걸쳐 소음, 분진, 용접흄, 중금속, 유기용제 등 다양한 유해환경에 노출되고 있다. 이 연구의 목적은 서울특별시 공업계열 특성화고 전문교과 교사를 대상으로 실험 · 실습실의 유해환경 요인에 대한 교사들의 인식수준을 분석함으로써, 특성화고 실험 · 실습실 유해환경 요인에 대한 교육자료 및 유해환경 요인 평가모델 개발을 위한 기초자료를 개발하는 것이다. 이를 위해 이 연구에서는 실험 · 실습실의 안전 및 환경에 대한 선행 연구문헌과 관련 법령을 검토하였으며, 서울지역 공업계역 특성화고 교사들을 대상으로 실험 · 실습실 유해환경 요인에 대한 교사들의 인식 조사 설문지를 개발하였다. 이 설문지는 실험 · 실습실의 현황, 실험 · 실습실에서 수업 시 교사가 유해환경 요인을 인식하는 정도와 교사들이 노출되는 물리적, 화학적, 인간공학적 유해환경 요소, 실험 · 실습실의 유해환경 개선 사업에 대한 교사의 인식 등을 조사하는 항목으로 구성되었으며, 서울지역 공업계열 특성화고 교사 348명이 설문에 응답하였다. 설문조사 결과 실험 · 실습실별로 교사가 인식하는 유해환경 요인의 종류와 인식 정도에는 큰 차이가 있었다. 실험 · 실습 과정에서 장비를 많이 사용하거나 화학물질을 사용하는 분야의 교사일수록 유해성을 크게 인식하는 것으로 나타났으며, 눈이나 코와 같이 바로 느낄 수 있는 유해환경 요소에 대해서는 유해성에 민감하게 인식하였으나 보이지 않는 유해환경 요소에 대해서는 유해성을 크게 인식하지 못하는 것으로 나타났다. Technical high school teachers are exposed to various hazardous environments such as noise, dust, welding fumes, heavy metals, and organic solvents over a long period of time because most of the classes are taught in practice rooms, except for work hours and classes focused on theory. The purpose of this study is to analyze the level of perception of teachers on the hazardous environmental factors in technical high schools in the Seoul City. In addition, it provides basic data for the development of educational materials for hazardous environment factors and evaluation models in technical high school practice rooms. To this end, this study reviewed previous research literature and related laws on safety and environment in the practice room and a questionnaire was developed to survey teachers" perceptions of hazardous environmental factors in the practice room of technical high schools in the Seoul. This questionnaire is for surveying the current status of the practice room, the degree to which the teacher perceives hazardous environmental factors in the practice room, the physical, chemical, ergonomic hazardous environment factors exposed to teachers, and the teacher"s perception of the hazardous environment improvement project in the practice room. And 348 teachers in Seoul area responded to this questionnaire. As a result of the survey, there was a big difference in the type and degree of recognition of hazardous environmental factors recognized by teachers for each practice subject. In the course of practice, teachers in fields that use more mechanical equipment or chemicals were found to be more aware of the hazard. In addition, the hazardous environmental factors that teachers can feel with their eyes or nose were sensitively perceived the hazard. However, it was found that the hazard was largely not recognized for the invisible hazardous environmental factors.

인간의 말초혈액에서 염증성 사이토카인에 의한 Th17 세포의 분화에 미치는 영향

허유정 ( Yu Jung Heo ),박미경 ( Mi Kyung Park ),주지현 ( Ji Hyeon Ju ),박경수 ( Kyung Su Park ),조미라 ( Mi La Cho ),김호연 ( Ho Youn Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.2

Objective: IL-17-producing T cells (Th17 cells) have been identified as a distinct lineage of CD4+ T helper cells in mice. Since this discovery, many efforts have been made to investigate the characteristics and the role of human Th17 cells and the factors involved in their differentiation. This study was undertaken to assess the effects of cytokines and stimulatory conditions on the differentiation of human CD4+ T cells into Th17 cells. Methods: Peripheral blood CD4+ T cells were isolated from healthy humans and then these cells were cultured with using various stimulatory conditions. The Th17 cells and regulatory T (Treg) cells were detected by flow cytometry (FACs). The related gene expressions of cytokines, transcription factors and chemokine receptors were determined by ELISA and RT-PCR. Results: In the presence of inflammatory cytokines, TNFa and IL-1b, the human CD4+ T cells rapidly produced IL-17 in response to anti-CD3/anti-CD28 stimulation, whereas, with anti-CD3/ anti-CD28 stimulation alone, the CD4+ T cells expressed low levels of IL-17. TNFa and IL-1b were also important inducers of IL-22 production. IL-6 and IL-23 up-regulated the RORgammat, CCR4 and CCR6 expressions in the human CD4+ T cells. In response to TGF-b and IL-2, the human CD4+ T cells were rapidly induced to express FoxP3, IL-10 and CCR7, as compared with anti-CD3/anti-CD28 stimulation alone. Conclusion: The effect of inflammatory cytokines on the differentiation of human Th17 cells may help us to understand their pathogenic role. Moreover, the differential expression of chemokine receptors and transcription factors of the subsets of CD4+ T cells with the different features of Th17 and Treg, may raise new issues concerning the pathogenesis of autoimmune inflammatory diseases.

참억새로부터 분리된 S-Adenosylmethionine Synthetase (SAMS) 유전자를 이용한 담배 형질전환체계 확립

Mi Ran Jeon,Eun Soo Seong,Ji Hye Yoo,Jae Hoo Choi,Chang Heum Kim,Byeong Ju Kang,Kweon Heo,Chang Yeon Yu 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10

Background : Temperature is major factor for growth plant. Recently, because of global warming, abnormal temperature included drought, deluge, sudden temperature change and heavy snow damaged crops in the world. In Korea, crops have been sensitive to low temperature on early growth stage, e.g. fruit tree and ginseng, were damaged owing to sudden heavy snow and cold on Spring. Therefore, recently interest in cold resistance crops were increased in demand rapidly. This study was performed to establish transgenic Nicotiana benthamiana by transforming cold resistant gene related to cold tolerance S-adenosylmethionine synthetase (SAMS) isolated from Miscanthus sinensis. Methods and Results : Total RNA was extracted from leaves of M. sinensis using Trizol assay and isolated MsSAMS. Isolated MsSAMS was insert into SacⅠ- XbaⅠ sites of pMBP1 vector. The vector was transformed to Agrobacterium tumefaciens strain LBA4404 by DH5α. A. tumefaciens with binary plasmid were selected at YEP medium supplemented with kanamycin. Cut leaves of tobacco were co-cultured with selected A. tumefaciens. Co-cultured leaves was grown on regeneration medium for a month at dark condition, and transferred to at light condition. Regeneration shoot from callus were excised and transferred to root-induction medium. Approximately, 58% of leaves explant produced callus. Nearly, 30% of callus had shoot and approximately, 94% of shoots were rooted in root-induction medium. Conclusion : We established an efficient transformation system of N. benthamiana transformed by using MsSAMS gene related to cold tolerance isolated from M. sinensis. We may use the produced transgenic plants to prevent damages carried by cold.

Characterization of SHORT-ROOT Function in the Arabidopsis Root Vascular System

Yu, Nan-Ie,Lee, Shin-Ae,Lee, Mi-Hyun,Heo, Jung-Ok,Chang, Kwang-Suk,Lim, Jun Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.2

Development of the vascular tissues is a dynamic process that integrates extrinsic and intrinsic factors to control vascular tissue formation throughout the plant life cycle. During vascular tissue formation in Arabidopsis roots, radial and longitudinal signals, including nuclear factors and plant hormones, control the developmental processes involved in the specification, differentiation, and maintenance of the correct cell types. SHR, a GRAS transcription factor, has been known to regulate the specification of the stem cell niche and ground tissue identity in the root meristem in a non-cell-autonomous manner. However, the role of SHR in the root vasculature is relatively overlooked, despite localization of its mRNA and protein in the stele. Here, we investigated the role of SHR in the vascular system of the primary root using a reverse genetic approach and detailed phenotypic analysis. A novel, loss-of-function mutant, shr-6, was isolated in the Columbia background, and vascular patterning was characterized in detail. Our results reveal that shr mutants have developmental defects in both protophloem and protoxylem elements. Our study also suggests that SHR plays a central role in the root vascular system to control patterning processes, possibly regulated by longitudinal and radial signals.

Comparison of Physiological Activities using Different IT Accessions of Sorghum bicolor M. in National Agrobiodiversity Center

Mi Ran Jeon(전미란),Eun Soo Seong(성은수),Ji Hye Yoo(유지혜),Jae Hoo Choi(최재후),Chang Heum Kim(김창흠),Byeong Ju Kang(강병주),Na Young Kim(김나영),Kweon Heo(허권),Chang Yeon Yu(유창연) 한국약용작물학회 2016 한국약용작물학회 학술대회논문집 Vol.2016 No.1

농산물중의 잔류농약 실태에 관한 연구(Ⅰ) : 고체상 추출에 의한 농산물중 잔류농약 분석에 관한 연구 Analysis of Residual Pesticides in Agricultural Products by Solid-Phase Extraction

허옥순,이재관,이정성,서정혁,주인선,허수정,김세은,김정근,신영희,유미자,김지연,심규창,김성환 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

신속 · 정밀하고 효율적인 짠류농약 검출을 위하여 극싱칼럼 (Extrelut-3')을 사옹한 SPE법을 시도하였다. 시료는 채소류체 사용하는 농약중 잔류성이 있고 식품공전상 시험 전처리가 각각 다른 Chlorothalonil등 6종의 농약을 선정하고 그들 표준액에 대한 PPIR수준의 농도로추출용라의 종류 및 용매량 등의 추출조건을 달리하여 회수율 등을 측정한 결과 최적의 분석조건을 얻었다. 1) 추출 용매량은 증가할수록 추출 수율이 높았고,용매 종류에 대한 추출수율은 각 농약별로 다소 차이는 있었으나 극성 정도가 비교적 높은 Ethyl acetate애서 평균 추출수율이 가장 높았다 2) 최대 평균회수율은 Ethyl acetate 60mL를 사웅할 경우로서 93.6%였다. 3)표준액을 시료에 첨가한 시험에서도 역시 Ethyl ace늘to가 푼출수율이 가장 높았으며 불순물 분리제거 효과도 LLE법 보다 월등히 우수하였다. 4) 각 농약별 검출한계는 치소 0.001ppin으로 농산물 중 미량 잔류하는 농약을 검출할 수 있을 것으로 사료된파. 그러므로 SPE법은 LLE법보다 경제적인 방법으로 판명되었으며, 앞으로 농산물 중 잔류농약 분석에서 LEE법을 대치할 쑤 있을 것으로 기대된다. Solid-phase extraction by polar column(Extrelut-3') was attempted to develope the fast and efficient method of detecting pesticide of farm product.5·ix kinds of pesticide used in farming fieldfrequently and different in pretreatment, were chosen from Korea Food Cord. Optfmal analysis condi-tions were determiBed from t31e recovery rate of standard pesticides according to extraction solvent andextraction volurae. Extraction yietd was increased as solvent polarity and extraction volume. Maximumrecovery rate was acquired at 93.6% when 60mL of ethyl acetate was used as eluent. Tllis method wasmore effective than liquid-liquid partition extract·ion method to eliminated the impurity and had 0.001ppm of detection limit. Therefore. solid-phase eEl=raction was expected to be economical substitute fortraditional liquid-liquid extT·action method.

2기 매독 환자에서 발생한 간질성 폐렴 양상의 Jarisch-Herxheimer 반응 1예

허중연,노지윤,김미정,최원석,조유미,송준영,정희진,김우주 대한감염학회 2009 감염과 화학요법 Vol.41 No.5

Jarisch-Herxheimer reaction (JHR) is a self-limited, acute febrile hypersensitivity reaction that occurs after antibiotic therapy against spirochetes disease. When occurring in the text of syphilis therapy, the JHR begins typically 1-2 hour after the administration of penicillin and is characterized by fever, chills, myalgias, and exacerbation of skin lesions. Rarely, severe JHR can occur in the form of endocarditis, fulminant hepatitis, and hypersensitivity pneumonitis. Recently, we experienced an interesting case of JHR complicated by the hypersensitivity pneumonitis after treating secondary syphilis. Proper differential diagnosis is required to differentiate this reaction from drug-induced hypersensitivity reaction.