Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

광섬유 집합체 센서를 이용한 6MV 광자선의 반음영 및 심부선량백분율 측정

신상훈,장경원,조동현,유욱재,서정기,이봉수,문주현,박병기 제주대학교 방사선응용과학연구소 2008 방사선응용과학연구소논문집 Vol.22 No.-

In this study, a fiber-optic bundle sensor has been developed using water-equivalent organic scintillating fibers for photon beam measurement. Beam penumbra and percent depth doses(PDD) are measured according to the depth of the photon beam. This sensor has many advantages such as high resolution, real-time measurement and ease of calibration over conventional radiation measurement devices.

[논문]다른 온도로 소성한 왕겨를 콘크리트 흔화재로 사용한 콘크리트의 강도특성에 관한 연구

신상엽,정의창,정유진,박문석,백계승,최세윤,김영수 釜山大學校生産技術硏究所 2007 生産技術硏究所論文集 Vol.66 No.-

본 논문은 다른온도로 소성한 왕겨를 콘크리트 흔화재로 사용한 콘크리트의 강도특성을 알아보기 위한 것으로 물결합재비를 35% 로 고정하고 왕겨의 혼입률을 각기 달리하여 소성온도에 따른 압축강도 특성과 왕겨의 혼입률에 따른 강도특성을 평가하였다. 실험결과 왕겨가 혼화재로서 성능을 발휘하는 최저 온도가 600℃인 것으로 나타났으며, 700℃로 소성한 경우 가장 우수한 강도 값을 나타내었다. 또한 혼입률별 압축강도 특성에서는 혼입률 10% 에서 가장 우수한 강도발현을 나타내었다.

Acriflavine-Guanosine 복합제 (AG60)의 조직내 분포에 대한 형광현미경적 연구

고정식,임수재,안의태,김진국,김상건,이경영,강종구,홍은경,정영신,유보림,한영복 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

중학교 가정교과의 열린교육 활성화를 위한 수업 모듈 및 웹 기반 교육자료 개발

신상옥,유태명,한규정,윤지현,홍정숙,김유정,양정혜 한국 가정과 교육 학회 2000 한국가정과교육학회지 Vol.12 No.3

This study is about open and secondary level Home Economics Education which is applicable to field and which includes the characteristics, purpose and problems of each open education. secondary education and home economics education. Based on the ideology of open education, this study will identify the meaning of openness in terms of Home Economics Education reconstructing meaning. purpose, characteristics, curriculum paradigm and curriculum. The instructional model and module which use web, will be Presented as the results of the reconstruction works. The open instructional module in Home Economics Education, which will be operated on the web, is consisted of 1)setting the orientation of curriculum development, 2)instructional model development. 3)rebuilding the Home Economics Education curriculum 4)development of instructional modules and web-based materials and 5)construction of the web-site . Six instructional module begin with critical/emancipatory curriculum Paradigm in open Home Economics Education. use module as a unit of class management instead of 1 class. facilitates nest-type integration which is applied from open education keeping the nature of Home Economics Education. and focus on practical problem and problem solving through reconceptualization with dialectical perspective on individual, family and society. The practical problem solving model has four steps : Identifying practical problems. Understanding the contexts of Problem, Searching for solutions and examining its consequences . Action and evaluation . The web-site has to be developed based on the six instructional module includes those issues : self-identification for reinforcing autonomy and self-esteem. living with famity. living with friends, autonomous living. healthy living, and understanding sexuality.

太白山의 나방相에 關하여

申裕恒,鄭明淑,金聖秀 慶熙大學校 1990 論文集 Vol.19 No.-

1. The authors made faunal study on the moths of Mt. T'aebaek, during the periods of May 21-24, June 23-27 and July 23-26 in 1987 respectively. 2. Hitherto 114 species of 17 families of the moths were recorded from this area. During the survey the authors collected 298 unrecorded species of 20 families of the moths from this area. Therefore, a total of 412 species of the moths are known at Mt. T'aebaek. 3. The following ten species are recorded newly from Korea. Pyralidae Eurrhypara hortulata(Linnaeus) Geometridae Rheumaptera Hedemannaria(Oberthu¨r) Cabera exanthemata (Scopoli) Cleora insolita (butler) Epione emundata Christoph Epiplemidae Epiplema plagifara (Butler) Notodontidae Lophontosia cuculus (Staudinger) Noctuidae Hermonassa arenosa Butler Dysaletia inanis (Oberthu¨r) Hapalotis venustula Hu¨bner

Al-Mg합금(Al-5wt%Mg-3wt%Zn-1wt%Mn)의 T6 열처리에 따른 미세조직 및 기계적 특성변화

유정훈,신기삼,허성강,김정민 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-

Al-Mg base alloys are attractive for aircraft. automobile and leisure applications because of their properties of low density, high intensity and wear resistance. The effect of Zn and Mn on the properties was studied in an Al-Mg base alloy. After T6 heat-treatment, the change of the microstructure and mechanical property was examined. Our investigations show that after T6 heat-treatment, the elongation was improved. The microstructures are investigated by TEM, which shows that the size of Al6Mn, Al57Mn12 phase is 100~500nm, and it also appears presence of the very small size MgZn₂ phase about 30nm.

성공적인 마을공동사업의 운영특성 분석-충청남도와 충청북도를 중심으로-

정상숙, 윤성수, 장우석, 리신호 忠北大學校 農業科學硏究所 2019 農業科學硏究 Vol.35 No.2

본문참고

企業 附設 硏究所 活動實態에 관한 硏究 : 釜山·慶南地域을 中心으로

河正鎭,黃圭完,申大赫,兪炳哲 동아대학교 공과대학 부설 한국자원개발연구소 1990 硏究報告 Vol.14 No.2

The research institute of an enterprise attaches have been enlarged in korea. This study grasps the status and the research activity in the research institute of an enterprise attached in the Pusan and Kyungnam. So, we find the important role in the research institute of a enterprise attached.

방사성 페기물 처리장의 지하수 온도 측정을 위한 광섬유 센서의 제작 및 특성 분석

서정기,유욱재,장경원,조동현,신상훈,이봉수 제주대학교 방사선응용과학연구소 2008 방사선응용과학연구소논문집 Vol.22 No.-

Fiber-optic temperature sensing technology can alternative to classical measurement techniques in nuclear environment, Its advantages allow low cost, a long distance measurement and real-time, In this study, we have measured the optical power of power meter and the output voltage of photodiode according to change temperature of water.