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Chae Yeon Lee,A-Reum Kang,Kaengkan Phatcharida,Yong-Won Choi,Kyung Yoon Kam,Byung-Rok Do,Sang Hae Kim,Sung Goo Kang 한국발생생물학회 2010 한국발생생물학회 학술발표대회 Vol.29 No.-
Mesenchymal stem cells (MSCs) has been reported as multipotent progenitor cells that can be expanded rapidly in vitro and differentiated into multiple mesodermal cell type. Human MSCs have been reported to be associated with neural differentiation especially in the cholinergic phenotype in several neural system. In this study, We investigated the ability of MSCs derived human aipose tissue to differentiation into neural cells expressing Islet-1 and further differentiates into cholinergic neurons in cholinergic differentiation media. Immunocytochemistry was performed to detect the expression of Islet-1 and demonstrate characteristic of neurons and cholinergic neurons. Islet-1 was massively detected in the induction stage. Following cholinergic differentiation from Islet-1-expressing MSCs, Cholinergic neuron marker ChAT was higly expressed. Also we examined the neuroprotective effects and neural differentiation of transplanted human adipose tissue-derived mesenchymal stem cells (AT-MSCs) in ischemic stroke. For transplantation, after 3days after MCAO. animal were divided into 2 group: Group A : injected phosphate buffered saline (PBS;5 ㎕ n=10), Group B: transplanted AT-MSCs (5×105 cells, n=10). Each animal received an injection into the right penumbra region (from bregma : AP;-1.3 ㎜, ML;-4.0 ㎜, DV;-5.9 ㎜). In all animals, behavior test were performed at 1, 3, 6, 9, 12, 15 days after MCAO, that was conducted by investigators who were blined to the experimental groups. mNSS test demonstrated that motor, sensory, and balance behavior were impaired after MCAO ischemic insult. Ischemic rats that received AT-MSCs exhibited significantly improved functional performance compared with PBS injected animals and histological analysis revealed that transplanted AT-MSCs expressed marker for neuron. These results suggest that AT-MSCs can be differentiated into neuron especially in cholinergic neuron and may be a potential source of treatment for neurodegenerative disease such as stroke.
A Bacterial Metabolite, Compound K, Induces Programmed Necrosis in MCF-7 Cells via GSK3β
( Chae Won Kwak ),( Young Min Son ),( Min Jeong Gu ),( Girak Kim ),( In Kyu Lee ),( Yoon Chul Kye ),( Han Wool Kim ),( Ki Duk Song ),( Hyuk Chu ),( Byung Chul Park ),( Hak Kyo Lee ),( Deok Chun Yang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.7
Ginsenosides, the major active component of ginseng, are traditionally used to treat various diseases, including cancer, inflammation, and obesity. Among these, compound K (CK), an intestinal bacterial metabolite of the ginsenosides Rb1, Rb2, and Rc from Bacteroides JY-6, is reported to inhibit cancer cell growth by inducing cell-cycle arrest or cell death, including apoptosis and necrosis. However, the precise effect of CK on breast cancer cells remains unclear. MCF-7 cells were treated with CK (0-70 μM) for 24 or 48 h. Cell proliferation and death were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. Changes in downstream signaling molecules involved in cell death, including glycogen synthase kinase 3β (GSK3β), GSK3β, β-catenin, and cyclin D1, were analyzed by western blot assay. To block GSK3β signaling, MCF-7 cells were pretreated with GSK3β inhibitors 1 h prior to CK treatment. Cell death and the expression of β-catenin and cyclin D1 were then examined. CK dose- and time-dependently inhibited MCF-7 cell proliferation. Interestingly, CK induced programmed necrosis, but not apoptosis, via the GSK3β signaling pathway in MCF-7 cells. CK inhibited GSK3β phosphorylation, thereby suppressing the expression of β-catenin and cyclin D1. Our results suggest that CK induces programmed necrosis in MCF-7 breast cancer cells via the GSK3β signaling pathway.
( Byung Ho Lee ),( Ho Won Seo ),( Myeong Yun Chae ),( Kyu Jeong Yeon ) 한국응용약물학회 2004 Biomolecules & Therapeutics(구 응용약물학회지) Vol.12 No.1
N/A The vascular relaxant effect of amlodipine adipate, a new salt of amlodipine, was evaluated in isolated rat aorta, and compared with that of amlodipine besylate. Furthermore, antihypertensive effects were measured in hypertensive rat models, such as spontaneously hypertensive rats(SHR) and renal hypertensive rats(RHR). Amlodipine adipate concentration-dependently inhibited Ca^(2+)-induced contraction of rat aorta with a very slow onset of action (reached its maximum at 3.5h; IC_(50): 3.76 nM), having a pattern and a potency similar to those of amlodipine besylate (IC_(50): 4.01 nM). In SHR and RHR, orally administered amlodipine adipate produced at dose-dependent and long-lasting(>10-24 h) antihypertensive effect(ED,,,:2.48 and 1.57 mg/kg, respectively). with a pattern and a potency similar to those of amlodipine besylate(ED_(20):2.50 and 1.90mg/kg in SHR and RHR, respectively). These results suggest that amlodipine adipate is a potent and long-lasting antihypertensive agent and that its antihypertensive effect is not significantly different to that of amlodipine besylate.
( Byung Ju Kang ),( Youn Suck Koh ),( Chae Man Lim ),( Jin Won Huh ),( Myong Ja Han ),( Hyun Suk Seo ),( Sang Bum Hong ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: High fiow nasal cannula (HFNC) provides a high fiow of humidified and heated oxygen. The use of HFNC allows respiratory failure patients required mechanical ventilation a chance to delay intubation. We compared the hospital outcomes of critical ill patients with HFNC therapy, early failure or late failure. Methods: We performed a retrospective observational study of patients receiving HFNC therapy in a tertiary hospital between January 2013 and March 2014. We selected intubated patients for the failure of HFNC therapy and classified the patients into two groups depending on the timing of intubation, intubation before and after 48 hours. Results: A total of 615 patients receiving HFNC therapy were enrolled in our study. Among them, 175 patients clinically deteriorated despite HFNC therapy and finally received endotracheal intubation. Before 48 hours, 130 patients (74.3%) were intubated and after 48 hours, 45 patients (25.7%) were intubated. There were no significant differences in baseline characteristics between two groups except higher diabetes mellitus (33.85% vs. 15.56%, p=0.02) and Sequential Organ Failure Assessment (SOFA) score day 1 (9.81±3.82 vs. 8.07±3.85, p=0.009) in the patients with intubation before 48 hours. The patients with intubation before 48 hours had higher extubation (37.69% vs. 15.56%, p=0.006) and ventilator weaning rate (55.38% vs. 28.89%, p=0.002), lower ICU mortality (39.23% vs. 66.67%, p=0.001), and longer ventilator free days (8.58±10.06 vs. 3.62±7.48, p=0.011). Conclusions: In a patient with clinically deterioration during HFNC therapy, delayed failure may cause bad outcome and harmful.
( Byung Ju Kang ),( Younsuck Koh ),( Chae Man Lim ),( Jin Won Huh ),( Myong Ja Han ),( Hyun Suk Seo ),( Sang Bum Hong ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.0
Background: High flow nasal cannula (HFNC) provides a high flow of humidified and heated oxygen. The use of HFNC allows respiratory failure patients required mechanical ventilation a chance to delay intubation. We compared the hospital outcomes of critical ill patients with HFNC therapy, early failure or late failure. Methods: We performed a retrospective observational study of patients receiving HFNC therapy in a tertiary hospital between January 2013 and March 2014. We selected intubated patients for the failure of HFNC therapy and classified the patients into two groups depending on the timing of intubation, intubation before and after 48 hours. Results: A total of 615 patients receiving HFNC therapy were enrolled in our study. Among them, 175 patients clinically deteriorated despite HFNC therapy and finally received endotracheal intubation. Before 48 hours, 130 patients (74.3%) were intubated and after 48 hours, 45 patients (25.7%) were intubated. There were no significant differences in baseline characteristics between two groups except higher diabetes mellitus (33.85% vs. 15.56%, p=0.02) and Sequential Organ Failure Assessment (SOFA) score day 1 (9.81±3.82 vs. 8.07±3.85, p=0.009) in the patients with intubation before 48 hours. The patients with intubation before 48 hours had higher extubation (37.69% vs. 15.56%, p=0.006) and ventilator weaning rate (55.38% vs. 28.89%, p=0.002), lower ICU mortality (39.23% vs. 66.67%, p=0.001), and longer ventilator free days (8.58±10.06 vs. 3.62±7.48, p=0.011). Conclusions: In a patient with clinically deterioration during HFNC therapy, delayed failure may cause bad outcome and harmful.