기관내 삽관 후 발생한 기관 괴사와 협착증 : 증례보고 A case report

권진형,구자욱,유시현 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Tracheal necrosis and stenosis are rare complications of prolonged endotracheal intubation. A 62 years old female was performed cerebral aneurysmal neck clipping under general anesthesia via endotracheal intubation and transferred to the 12th intensive care unit. The patient was mechanically ventilated and tracheostomy was done on the postoperative day. Necrotic tissue, pus and stenosis were found around the ballooning site of the trachea. T-tube insertion was done on the both postoperative day and the patient was discharged on the 80th postoperative day.

Excitatory neuron–specific SHP2-ERK signaling network regulates synaptic plasticity and memory

Ryu, Hyun-Hee,Kim, TaeHyun,Kim, Jung-Woong,Kang, Minkyung,Park, Pojeong,Kim, Yong Gyu,Kim, Hyopil,Ha, Jiyeon,Choi, Ja Eun,Lee, Jisu,Lim, Chae-Seok,Kim, Chul-Hong,Kim, Sang Jeong,Silva, Alcino J.,Kaang AAAS 2019 Science signaling Vol.12 No.571

<P><B>Cell type–specific RASopathy</B></P><P>The neurodevelopmental disorder Noonan syndrome is often caused by activating mutations in the phosphatase SHP2 that enhance RAS signaling. However, SHP2 is present in multiple neuron types as well as glia; thus, where the mutant protein has its pathological effects is unclear. Ryu <I>et al.</I> examined one NS-associated SHP2 mutation in isolated cell types from mice and determined that its presence in only excitatory neurons resulted in electrophysiological and cognitive effects. This was because certain adaptor proteins that interact with SHP2 to mediate RAS signaling are abundant in excitatory but not inhibitory neurons. These findings reveal that cell type–specific variations within the RAS signaling network underlie the phenotypes of NS and possibly other “RASopathies”.</P><P>Mutations in RAS signaling pathway components cause diverse neurodevelopmental disorders, collectively called RASopathies. Previous studies have suggested that dysregulation in RAS–extracellular signal–regulated kinase (ERK) activation is restricted to distinct cell types in different RASopathies. Some cases of Noonan syndrome (NS) are associated with gain-of-function mutations in the phosphatase SHP2 (encoded by <I>PTPN11</I>); however, SHP2 is abundant in multiple cell types, so it is unclear which cell type(s) contribute to NS phenotypes. Here, we found that expressing the NS-associated mutant SHP2<SUP>D61G</SUP> in excitatory, but not inhibitory, hippocampal neurons increased ERK signaling and impaired both long-term potentiation (LTP) and spatial memory in mice, although endogenous SHP2 was expressed in both neuronal types. Transcriptomic analyses revealed that the genes encoding SHP2-interacting proteins that are critical for ERK activation, such as GAB1 and GRB2, were enriched in excitatory neurons. Accordingly, expressing a dominant-negative mutant of GAB1, which reduced its interaction with SHP2<SUP>D61G</SUP>, selectively in excitatory neurons, reversed SHP2<SUP>D61G</SUP>-mediated deficits. Moreover, ectopic expression of GAB1 and GRB2 together with SHP2<SUP>D61G</SUP> in inhibitory neurons resulted in ERK activation. These results demonstrate that RAS-ERK signaling networks are notably different between excitatory and inhibitory neurons, accounting for the cell type–specific pathophysiology of NS and perhaps other RASopathies.</P>

A study of the human skin-whitening effects of resveratryl triacetate.

Ryu, Ja Hyun,Seok, Jin Kyung,An, Sang Mi,Baek, Ji Hwoon,Koh, Jae Sook,Boo, Yong Chool Springer-Verlag 2015 Archives of dermatological research Vol.307 No.3

<P>Resveratrol has a variety of bioactivities that include its anti-melanogenic effects, but its use in cosmetics has been challenging partly because of its chemical instability. Resveratryl triacetate (RTA) is a prodrug that can enhance stability. The purpose of this study was to examine the skin safety and whitening effects of RTA in human subjects. The primary skin irritation potentials of RTA and resveratrol were tested at 0.1 and 0.5 % on human subjects. Resveratrol at a concentration of 0.5 % induced weak skin irritation, whereas RTA did not induce any skin responses. The skin-whitening efficacy of a cosmetic formulation containing 0.4 % RTA was evaluated in two different test models. In the artificial tanning model, the test product and the control product were applied twice daily to the skin of the forearms of 22 human subjects after pigmentation induction by ultraviolet irradiation. Applying the test and the control products to the artificial tanning model for 8 weeks increased the individual topology angles (ITA) by 17.06 and 13.81 %, respectively, a difference that was statistically significant (p < 0.05). In the hyperpigmentation model, the test product and the control product were applied twice daily to the faces of 21 human subjects. The averaged intensity of the hyperpigmented spots decreased by 2.67 % in the test group and 1.46 % in the control group, a difference that was statistically significant (p < 0.05). Therefore, RTA incorporated into cosmetic formulations can whiten human skin without inducing skin irritation.</P>

한국여성에서 자간전증과 peroxisome proliferator-activated receptor-γ, methylenetetrahydrofolate reductase 유전자 다형성의 연관성

이소현 ( So Hyun Lee ),박보현 ( Bo Hyun Park ),박미혜 ( Mi Hye Park ),박혜숙 ( Hye Sook Park ),전선희 ( Sun Hee Chun ),안정자 ( Jung Ja Ahn ),류현미 ( Hyun Mee Ryu ),이광수 ( Kwang Soo Lee ),박현영 ( Hyun Young Park ),김영주 ( You 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.4

목적: 자간전증의 발병기전에 관여할 것으로 예측되는 PPARγ와 MTHFR 유전자의 단일염기다형성 및 일배체형과 한국인에서 자간전증의 연관성을 분석하고자 하였다. 연구 방법: 자간전증 환자 226명과 대조군 235명을 대상으로 하여 말초혈액검체에서 DNA를 추출하였고, PPARγ(-796A>G, P12A (C>G), H447H (161C>T)), MTHFR (A222V (677C>T), E429A (1298A>C), R594Q (1793G>A)) 유전자의 단일염기다형성의 유전자형은 SNaPShot assay kit를 이용한 single base primer extension assay로 분석하였다. 결과는 Student`s t-test, 카이제곱검정, 선형회귀분석으로 분석하였고, 일배체형 분석은 Haploview 3.2 version을 이용하여 수행하였다. 결과: 대상 환자들에서 PPARγ, MTHFR 유전자의 대립유전자 빈도 및 유전자형 빈도는 환자군과 대조군 간에 유의한 차이가 없었고 (p>0.05), PPARγ와 MTHFR의 단일염기다형성들의 유전자형은 자간전증 위험에 있어 유의한 차이가 없었다(p>0.05). PPARγ과 MTHFR 유전자의 단일염기다형성들 중 MTHFR 유전자의 3가지 단일염기다형성 677C>T, 1298A>C, 1793G>A 사이에 서로 강한 연관불균형을 보였으나 (Lod score>2.0), MTHFR 유전자의 TAG, CAG, CCA, CCG 일배체형의 유전자형의 분포는 환자군과 대조군 간에 유의한 차이가 없었고 (p>0.05) 자간전증 위험에 있어 통계적으로 유의한 차이가 없었다 (p>0.05). 결론: 이상의 결과를 통해 한국인에서 PPARγ과 MTHFR의 유전자다형성은 자간전증과 연관성이 없었고 이들의 일배체형도 연관성이 없었다. Objective: To investigate whether polymorphisms of genes encoding peroxisome proliferator-activated receptor-γ (PPARγ) and methylenetetrahydrofolate reductase (MTHFR) are associated with preeclmapsia in Korean women and also to demonstrate whether there is any haplotypic association between preeclampsia and those genes. Methods: DNA was extracted from whole blood of 226 preeclampsia patients and 235 healthy pregnant women. The genotypes of SNPs in PPARγ (-796A>G, P12A (C>G), H447H (161C>T)) and MTHFR (A222V (677C>T), E429A (1298A>C), R594Q (1793G>A)) were analyzed by a single base primer extension assay using a SNaPShot assay kit. Results were analyzed with the Student`s t-test, Chi-square test, and Logistic regression analysis. Haplotype analyses were performed using Haploview 3.2 version. Results: There were no significant differences in genotype or allele frequencies of PPARγ and MTHFR gene polymorphisms between preeclampsia patients and controls (p>0.05). No increase in the risk of preeclampsia for those genes was observed under any model of inheritance. Among SNPs of the PPARγ, MTHFR genes, only SNPs in MTHFR gene (677C>T, 1298A>C, 1793G>A) were in a strong linkage disequilibrium with each other (Lod score>2.0), but there were no significant differences in genotype distribution of haplotypes of MTHFR gene (TAG, CAG, CCA, CCG) between preeclampsia patients and controls (p>0.05). No statistically significant associations were observed between any haplotypes of MTHFR gene and preeclampsia risk. Conclusion: This study suggest that SNPs in PPARγ and MTHFR gene were not associated with preeclampsia in Korean women, and its haplotypes were also not associated with preeclampsia.

시판 멸치액젓의 품질평가 방법에 관한 연구

김재현(Jae-Hyun Kim),류기형(Gi-Hyung Ryu),안현주(Hyun-Joo Ahn),이경행(Kyoung-Haeng Lee),이현자(Hyun-Ja Lee),변명우(Myung-Woo Byun) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.5

시판 멸치액젓의 객관적 품질평가 및 그 기준에 대한 기초자료를 제시하고자 현재 국내에서 유통되는 6개사 멸치액젓의 미생물학적, 화학적, 물성학적 및 관능적 품질특성을 검토하였다. 식염농도의 경우 시판제품 모두 품질 규격보다 높게 나타났고, 수분함량 및 pH의 경우 적합하였다. 아미노태 질소, 휘발성 염기태 질소 및 TMA도 제품간의 함량 범위가 다양하게 나타났으며, 시판 제품중에는 아미노태 질소 함량이 규격치에 도달하지 못하고 관능적으로 부적합한 제품이 유통되는 것으로 나타났다. 미생물의 경우 식품에 존재시 위해 가능성이 있는 병원성 세균이 검출되어 품질의 안정화를 위한 위생화 기술이 요구되었다. 관능검사와의 상관관계에서 아미노태질소, 휘발성 염기태질소 및 물리적 특성인 점도가 상관성이 큰 것으로 나타나 품질평가의 기준으로 적합하였다. Commercially distributed salted and fermented anchovy sauces in Korea were purchased from six different companies. Microbiological, chemical, rheological and sensory analyses were carried out to evaluate the quality and safety and to provide basic information of the products. The salinity of every product was lower than that of regulatory standard. All samples tested had acceptable in moisture content and pH. However, wide range of amino nitrogen (AN), volatile basic nitrogen (VBN), and trimethylamine (TMA) contents were observed. One product among tested had lower level of AN than regulatory standard. Pathogenic micro- organisms were also detected from the samples, therefore sanitation procedure will be needed to ensure safety. From the results, AN, VBN, and viscosity were highly correlated to sensory scores, suggesting that these values can be utilized as a standard method to evaluate quality of the salted and fermented anchovy sauce.

Case Report : Severe ischemic bowel necrosis caused by terlipressin during treatment of hepatorenal syndrome

( Hae Rim Kim ),( Young Sun Lee ),( Hyung Joon Yim ),( Hyun Joo Lee ),( Ja Young Ryu ),( Hyun Jung Lee ),( Eileen L Yoon ),( Sun Jae Lee ),( Jong Jin Hyun ),( Sung Woo Jung ),( Ja Seol Koo ),( Rok Sun 대한간학회 2013 Clinical and Molecular Hepatology(대한간학회지) Vol.19 No.4

Terlipressin is a vasopressin analogue that is widely used in the treatment of hepatorenal syndrome or variceal bleeding. Because it acts mainly on splanchnic vessels, terlipressin has a lower incidence of severe ischemic complications than does vasopressin. However, it can still lead to serious complications such as myocardial infarction, skin necrosis, or bowel ischemia. Herein we report a case of severe ischemic bowel necrosis in a 46-year-old cirrhotic patient treated with terlipressin. Although the patient received bowel resection, death occurred due to ongoing hypotension and metabolic acidosis. Attention should be paid to patients complaining of abdominal pain during treatment with terlipressin.

Abdominal Obesity as a Risk Factor for the Development of Erosive Esophagitis in Subjects with a Normal Esophago-Gastric Junction

( Ja Seol Koo ),( Sang Woo Lee ),( Sun Min Park ),( Sung Woo Jung ),( Hyung Joon Yim ),( Jong Jae Park ),( Hoon Jai Chun ),( Hong Sik Lee ),( Jai Hyun Choi ),( Chang Duck Kim ),( Ho Sang Ryu ) 대한소화기기능성질환·운동학회 2009 Gut and Liver Vol.3 No.4

Background/Aims: Obesity is reported to be associated with erosive esophagitis (EE). However, the temporal association of obesity and abdominal obesity with EE is unclear. We conducted this study to investigate the temporal association of obesity, especially abdominal obesity with EE. Methods: Among 1,182 subjects who underwent health screening examinations including upper endoscopy in both 2003 and 2006, a total 1,029 subjects with a normal esophagogastric junction on upper endoscopy in 2003 were enrolled. All subjects completed questionnaires and anthropometric measurements were obtained twice by trained personnels. The patients with newly developed EE were compared to the subjects without newly developed EE. Results: Among 1,029 subjects, 42 (4.1%) were newly diagnosed with EE and 82 (8.0%) with hiatal hernia. The mean body mass index (BMI) in both examinations was significantly different between the two groups based on the development of erosive esophagitis (p<0.05 in both examinations). The mean waist circumference (WC) in both examinations was also significantly different between the two groups (p<0.01 in both examinations). The multivariate analysis demonstrated that EE was not associated with the BMI in 2003 and the increase of BMI; however, it was associated with the WC in 2003 (Odds ratio, 7.21; 95% CI, 1.78 to 29.19; >90 cm vs <80 cm). Conclusions: Our study showed that abdominal circumference is an independent risk factor for EE, demonstrating a temporal relationship between abdominal obesity and EE. (Gut and Liver 2009;3: 276-284)

Prognostic Value of Preoperative Metabolic Tumor Volume and Total Lesion Glycolysis Measured by ¹⁸F-FDG PET/CT in Salivary Gland Carcinomas

Ryu, In Sun,Kim, Jae Seung,Roh, Jong-Lyel,Lee, Jeong Hyun,Cho, Kyung-Ja,Choi, Seung-Ho,Nam, Soon Yuhl,Kim, Sang Yoon Society of Nuclear Medicine 2013 The Journal of nuclear medicine Vol.54 No.7

<P>Metabolic tumor volume (MTV) and total lesion glycolysis (TLG) from <SUP>18</SUP>F-FDG PET/CT are emerging prognostic biomarkers in various human cancers. This study examined the prognostic value of these metabolic tumor parameters measured by pretreatment <SUP>18</SUP>F-FDG PET/CT in patients with salivary gland carcinomas. <B>Methods:</B> Forty-nine patients with intermediate- or high-grade salivary gland carcinomas who underwent definitive surgery with or without radiotherapy or chemoradiotherapy were evaluated preoperatively by <SUP>18</SUP>F-FDG PET/CT. Maximum standardized uptake values (SUV<SUB>max</SUB>), MTV, and TLG were measured for each patient. Univariate and multivariate analyses were used to identify clinicopathologic and imaging variables associated with progression-free survival (PFS) and overall survival (OS). Univariate analyses included the following variables: age, sex, pT and pN classifications, overall pTNM stage, histologic grade, resection margin, tumor lymphovascular invasion and perineural invasion, postoperative adjuvant therapy, gross total volume, SUV<SUB>max</SUB>, MTV, and TLG. <B>Results:</B> The 3-y PFS and OS rates for all study patients were 66.9% and 81.6%, respectively. The median SUV<SUB>max</SUB>, MTV, and TLG were 5.1 (range, 1.7–21.5), 16.2 mL (1.0–115.1 mL), and 24.4 g (2.1–224.4 g), respectively. Univariate analyses showed that there were significant correlations between pT classification, pN classification, MTV, and TLG and both PFS and OS (<I>P</I> < 0.05). However, SUV<SUB>max</SUB> was not associated with either PFS (<I>P</I> = 0.111) or OS (<I>P</I> = 0.316). Multivariate analyses revealed that MTV (<I>P</I> = 0.011; hazard ratio, 11.50; 95% confidence interval, 1.45–91.01) and TLG (<I>P</I> = 0.038; hazard ratio, 3.55; 95% confidence interval, 1.07–11.76) were independent variables for PFS. <B>Conclusion:</B> Pretreatment values of MTV and TLG are independent prognostic factors in patients with intermediate or high-grade salivary gland carcinomas.</P>

Transformation Rate of Insecticide Spirotetramat to Its Metabolites in Perilla Leaves

Ja-Gun Kang,Jeong-In Hwang,Sang-Hyeob Lee,Se-Yeon Kwak,Min-Su Kang,Jun-Sang Ryu,Seong-Hyun Hong,Jang-Eok Kim 한국농약과학회 2017 한국농약과학회 학술발표대회 논문집 Vol.2017 No.4

노년기 주관적 기억력 감퇴의 예측인자 : 한국인의 건강과 노화에 관한 종적 연구 결과

유지민,김기웅,박준혁,이석범,최은애,최진영,정지운,도연자,노현아,박영선,이동영,우종인 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

Objectives : We investigated the predictors of subjective memory complaints in the community-dwelling normal elderly. Methods : This study was conducted as a part of Korean Longitudinal Study on Health and Aging (KLoSHA). 747 non-demented community-dwelling elderly aged 65 years or older were recruited. All participants underwent clinical evaluation for dementia and psychiatric disorder conformed to the Korean version of the Consortium to Establish a Registry for Alzheimer's Disease (CERAD-K.) Clinical Assessment Battery and Korean version of Mini-Intemational Neuropsychiatric Interview, res-pectively. Word list recall test, frontal assessment battery, Mini Mental Status Examination (MMSE-KC) and Korean version of Geriatric depression scale (GDS-K) were administered to evaluate episodic memory, frontal function, global cognition and depression, respectively. Subjective memory complaint was defmed in two different ways: worse than one's past (SMC-P) and worse than others of one's age (SMC-O). Results : In highly educated elderly, minor depressive disorder (0R=7.23, 95% C.I.= 2.29-22.86) and frontal dysfunction (0R=2.48, 95% C.I.= 1.29-4.77) significantly increased the risk of SMC-O. However, they did not influence the risk of SMC-P. In low educated elderly, both the minor depressive disorder and frontal dysfunction did not influence the risk of SMC-0 as well as that of SMC-P. Conclusion : SMC-O can be a sensitive subjective recognition of mild depression and/or frontal dysfunction in highly edu-cated normal elderly.