Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

림프질 전이가 있는 하인두암과 하부식도암의 진단에 활용된 내시경초음파 유도하 Trucut 생검 1예

광명옥,이정환,문정섭,김유선,류수형,총배천,김형훈,김현태 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

The incidence of double primary cancer is about 10% in all kinds of cancers. Double primary cancer is usually accompanied by gastrointestinal cancer. Esophageal cancer is supposed to be frequently accompanied by squamous cell carcinoma of the head and neck or the upper respiratory tract, because of such common carcinogens as smoking and alcohol ingestion. Esophageal cancer and hypopharyngeal cancer are usually diagnosed by esophagogastric endoscopy with biopsy. We performed endoscopic ultrasound-guided Trucut biopsy(EUS-TCB) to diagnose a mediastinal mass in a 49 year-old patient, who visited our hospital because of voice change. During procedure, we accidently found enlarged celiac lymph node, and obtained tissue from the mediastinal lymph node and the celiac lymph node. We confirmed double primary cancer of squamous cell carcinoma in the esophagus and the hypopharynx via esophagogastric endoscopy.

비만세포주에서 Histamine 유리에 관한 연구

김형룡,국윤아,신민,이홍수,원진숙,김형민,이영미,정헌택,이혜숙,안녕형 원광대학교 생명공학연구소 1995 생명공학연구소보 Vol.3 No.1

경영에서 grab start와 track start의 운동학적 분석

김연정,이민형,원문학,김헌수 한국운동역학회 2000 한국운동역학회지 Vol.10 No.1

본 연구는 남녀 수영 선수 6명씩을 대상으로 grab start와 track start를 실시하여 두 집단간과 두 출발법간의 동작 특성을 운동학적으로 분석한 후 변인들을 정량화 하여 선수들에게 출발법에 대한 심층적인 이해를 제공하고 효과적인 훈련에 기여하도록 하는 것이 목적이며 실험으로 카메라 4대를 이용하였으며 분석을 위해 권(1993)이 개발한 Kwon3D 동작 분석 프로그램으로 3차원 영상 분석을 실시하였다. 결과의 통계 처리 자료로는 SPSS 프로그램을 사용하였으며 출발에 관련된 운동학적 변인을 수평방향과 수직방향에서의 인체 중심점 위치와 속도의 변화를 측정하였다. 위치 변화에서는 출발대를 떠나는 지점에서 남자 선수들은 수직 지향을 여자 선수들은 수평 지향을 함을 알 수 있었고 속도 변화에서 출발대를 떠나는 순간 track start가 속도값이 크기는 하나 두 출발법 사이에 유의한 차이는 나타나지 않았다. The research has the aim to analyze and find out kinematic features of grab start and track start between male and femail. With having selected 6 swimmers form each for this experiment four video-cameras(60 frames/sec) and control-box(length-2m, width-1m, hight-1m) to get real space coordinates were used. The DLT method was employed to obtain the 3-dimensional coordinates of the 25 body points for digitizing with Kwon3D Motion Analysis Package developed by Kwon, Young-hoo (1993). SPSS was used for statistic process. Kinematic variable were measured in start: The center of mass of body in horizontal and vertical, variation of velocity, entry distance. The following are the results. 1. In change of the body-center, the starting methods of man and women are same in horizontal directions, but in vertical directions the amount of women´s body changement is decreasing ever, while the amount of man´s body changement is increasing continuously with his foot off the start line. The change of the position and velocity in vertical directions is changing with the same tendency in case of starting ways of both man and women, but in vertical directions man´s amount of changement has steep increasing curve, and women´s one has decreasing curve. 2. Horizontal ANOVA analysis of body-center shows that starting method represent not such difference at taking-off moment but significant difference at initial moving point(F=237.016, p<.01). Vertical analysis shows significant difference in gender and starting method(for each F=31.132, p<.01, f=22.591, p<.01)

난삭재인 STD11 강의 정면밀링가공시 절삭특성에 관한 연구

김형석,문상돈,오성훈 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2003 工學硏究 Vol.34 No.-

Wear and fracture mode of ceramic tool for hardened Std11 steel was investigated by face milling in this study. The cutting force and acoustic emission(AE) signal were utilized to detect the wear and fracture of ceramic tool. The following conclusions were obtained : (1) The wear and fracture modes of ceramic tool are characterized by the three types : ① wear which has normal wear and notch wear, ② wear caused by scooping on the rake face, ③ large fracture caused by thermal crack in the rake face. (2) The wear behaviour of ceramic tool can be detected by the increase of mean cutting force and the variation of the AE RMS voltage. (3) The catastrophic fracture of ceramic tool can be detected by the cutting force(Fz-component). (4) As the hardness of work material increased, acoustic emission RMS valve and mean cutting force(Fz) increased linearly, but the tool life decrease.

도로주변 낙하분진의 침착속도와 수계환경에 미치는 영향

김환기,송호면,김형훈 全北大學校 附設 都市및環境硏究所 1997 都市 및 環境硏究 Vol.12 No.1

The air pollutants were transported over the long distant by the wind and were deposited to the ground or water surface by the gravitational sedimentation, precipitaion or Brown movement and so on. In this case, the deposited particles might give a bad effect to the water environment, such as eutrophication, damage of aquatic organism. This study was carried out to compare the deposition flux to the 17th national road and Il-San lake, Kyunggi, and to investigate the principal organic components. The deposition flux of 17th national road was 228.2㎎/㎡ day from Feb. to June., and that of Il-San lake was 276.4㎎/㎡ day from Feb., to June. Each components exist in the ratio of COD : T-N : T-P = 100 : 10.8 : 0.9, and the ratio of COD to the VSS is 1.2. The ratio of VSS to TSS was 0.12, the ratio of NH_3-N to T-N was 0.48 and the ratio of PO_4-P to T-P was 0.25. The average deposition velocity was 4.2㎝/s in 17th national road and 4.9 ㎝/s in the Il-San lake. The minimum velocity site was forest at the rate of 1.0㎝/s.

마이크로-매크로 셀룰라 시스템을 위한 계층선정 기법분석

김장섭,이상헌,정우곤,최형진 成均館大學校 科學技術硏究所 1998 論文集 Vol.49 No.1

A control scheme to select the appropriate layer between macro- and microcell hierarchical structure is considered. With the assumption that the each mobile speed is known to the system. the algorithm determines the optimal velocity threshold by which the cell section performed. The optimal criterion is to minimize the Grade of Service(GOS), a service criterion in cellular telephone systems. Mobile station with faster speed relative to the determined optimal speed threshold are assigned to a macrocell, while slower moving terminals are assigned to the smaller cells, i,e.. microcells. For the evaluation purpose for the proposed scheme, two different evaluation perspectives are compared and the algorithm proves to work

계층구조 무선망 셀룰라 시스템에서 신호세기 측정을 이용한 이동국의 위치와 속도 추정에 관한 연구

이상헌,김장섭,정우곤,최형진 成均館大學校 科學技術硏究所 1998 論文集 Vol.49 No.1

Estimating the positron and velocity of mobile stations is an important issue tar hierarchical cellular systems as the efficient allocation of mobiles to macro or microcells depends on its position and velocity. rower measurement method is applied and discrete location path-loss information is used to estimate the position of the mobile. Velocity estimation is treated in the same way with the position estimation problem since the mobility is the movement in a unit time. Lowpass smoothing technique and moving average are applied. The results prove to enhance the performance of the system. The path-loss data used in the simulator 2D-tracing method. Direction of _Future research in the topics are presented.

휴대용 근적외분광분석기를 이용한 유탕면의 수분 함량 측정 : Infrared(NIR) System

임현량,김도형,우영아,김효진 동덕여자대학교 종합약학연구소 2002 동덕약학연구지 Vol.6 No.-

This experiment was carried out to determine non-destructively water content in a intact fried noodle in oil by portable near-infrared system. Water content was ranged from 0 to 14.3w/w% and the near infrared (NIR) measurements were made on 70 intact samples over the spectral range 1100-1750nm. We found the variation of absorbance band due to OH vibration of water in the noodles depending on the concentration change around 1450nm in the second derivatives spectra. Partial least squares(PLS) regression models were set up using a calibration set(42 E.A.) and a validation set(28 E.A.) between the NIR response and value from loss on dry. Various pretreatments of the spectra were examined: the smallest stnadard error of prediction(0.35%) was obtained using autoscaling spectra over 1100-1700nm. 10 factors were required for the PLS model to give a good relationship between the spectral information and the noodle water content. In order to validate the developed calibration model, routine analyses were performed using other intact noodles. Water contents from the NIR calibration model were compared with the values from loss on dry and the results showed good correlation with those of loss on dry. This study showed that the rapid and non-destructive determination of water content in a intact fried noodle in oil was successfully performed by portable NIR system.

백서에서 Depulpin®과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,김선호,황윤찬,오병주,황인남,김선헌,정선와,윤창,오원만 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

One fifth dilution of formocresol is usually for pulpotomy of the primary teeth and emergency pulpotomy of the permanent teeth. However, the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it may also alter the pulp tissue rendering it immumologically active, and have carcinogenic potential. Recently Depulpin®(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49% paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier. But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine. Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rats were sacrificed after 2 days, 4 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue, in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2 days, 4 days, 1 week, 2 weeks in which vital tissue was present. it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3 weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin® group The area of necrotic tissue which had no cells and fibers , was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nucleli. A short stump of vital pulp with odontoblasts was present at the end of the canal after 2 days. Inflammatory infiltrate was in the periodontal ligament after 4 days and after 1week. Severe root resorption and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3 weeks. Periapical lesion which consist of necrotic tissue surrounded by a fibrous connective wall. was found after 4 weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.