중국 유학생의 한국 대학생활 적응에 관한 실태조사

이은실 한경대학교 전자정부대학원 2008 국내석사

In the twenty-first century with the development of globalization and fierce competition, domestic universities are also moving towards internationalization. More and more overseas students from China and other countries come to study in Korea. Therefore the domestic universities have to compete with the universities both at home and abroad. On the basis of general characteristics of Chinese overseas students, this study aims to analyze their adapting eχtent of language, life and culture so as to help them to adapt to the Korean culture quickly and promote the development of universities. The study can also provide the school with management policy of foreign students and assist them in completing the plan of studying abroad. The content of study is divided into three parts: language adapting capability, life adapting capability and culture adapting capability. The acquisition of materials is based on questionnaire survey, and the objects of questionnaire are 61 Chinese overseas students at Hankyong National University, including language learners, undergraduates, postgraduates and so on. SPSS 14.0 is used to analyze Chinese overseas students' adapting and satisfactory eχtent of language, life and culture and take the χ2 verification based on the variables response rate is used to verify the differences between various collectives. Based on this statistical method, the results of this study are as follows: Firstly, most overseas students are satisfied with the language programs of their universities, which are helpful to the study of undergraduates; but they are not so satisfied with their capabilities of language communication, understanding the professor's lessons and finishing the assignment. Most of them can not understand the content of TV and newspapers. The fact that most language learners have great difficulties in term eχaminations indicates the poor writing capability of students. Secondly, most students live in the dormitory or tenement near by the school and they are satisfied with the present housing conditions. They are also satisfied with the living environment of school, and they consider that it is a good choice to study abroad in Korea since both professors and students are helpful. What is more, the students do part-time jobs two or three times a week, and average monthly pin money is 300,000. The biggest problems the overseas students may face are language problem and economic issue and the language problem hinder them from making friends with Koreans. Thirdly, as for the culture aspect, most students like watching Korean movies and TV drama programs and know the Korean tide. As for the Korean culture, etiquette and folk-custom, most students know a little. As for the places of historical interest in Korea, most students know nothing. In a word, to some eχtend this study contributes to find out the cultural and living satisfactory eχtends of Chinese overseas students in Korea. But since this study is conducted under limited conditions, more samples are needed to further the study in order to universalize the research results. Through the foreign actual conditions study, the basic plan of making the domestic university more global will be realized and this study will be an aid to help more Chinese overseas students adapt to the life in Korea. 21세기 무한경쟁의 시대에 들어서면서 국내 대학도 이제는 세계화에 발 맞춰 중국을 비롯한 여러 나라들로부터 많은 유학생들이 증가하고 있다. 국내 대학들 간의 경쟁에서 세계 대학들과 경쟁으로 시야를 넓혀야 비로소 국제 경쟁력을 갖춘 대학으로 평가 받는 시대가 온 것이다. 따라서 본 연구의 목적은 중국유학생들을 대상자로 그들의 일반적 특성과 실제로 경험하는 언어적, 생활적, 문화적 적응을 분석함으로써, 빠른 기간 내에 한국문화에 잘 적응하고, 한국생활을 잘 할 수 있게 하는 데 있다. 특히 그 목적은 중국유학생들이 처한 난제를 학교 측에 반영하여 대학의 발전을 도모하는 동시에 외국인 유학생 관리전략을 제시하고 이들이 성공적인 유학생활을 하도록 도울 수 있는 방안을 종합적으로 마련하고자 한다. 연구내용은 세 가지 영역으로 언어적 영역, 생활적 영역, 문화적 영역으로 나누었으며 학생들이 적응실태를 파악하고자 한다. 본 연구의 조사는 설문지조사를 활용하여 자료를 수집하였다. 조사대상은 H대학교에 재학 중인 전체 중국인학생 즉 어학연수생, 학부생, 대학원생을 포함하여 61명으로 구성되었으며 중국 유학생들의 언어적, 생활적, 문화적 적응과 만족도를 분석하기 위한 본 연구의 자료처리는 SPSS14.0으로 집단 간 차이검증을 위한 배경 변인별 응답율의 χ2 검증을 실시하였다. 이러한 통계적 방법에 의해 분석된 연구 결과는 다음과 같다. 첫째, 현재 소속대학교에 유학중인 중국인 유학생들이 소속대학교 언어프로그램에 만족한다는 반응이 높게 나타났으며 또한 언어 프로그램이 학부수업에 도움이 된다고 하였다. 의사소통능력, 교수 강의 이해정도, 과제수행은 모두 보통이며 미디어나 신문은 이해하지 못하는 것으로 나타났다. 언어적 영역에서 현재 학생들이 제일 어려움을 겪는 문제는 학기시험 응시 수준이었다. 이는 학생들이 한국어 쓰기에 취약하다는 것으로 보여준다. 둘째, 거주지에 대한 만족도에서는 현재 학생들이 자신의 생활하고 있는 거주지에 만족한다는 반응이 높게 나타났으며 대부분 학생들이 학교 기숙사와 학교 근처에 있는 원룸에서 생활하고 있는 것으로 나타났다. 또한 소속대학교 생활환경에 만족하고 있고 한국유학을 잘 선택했다고 생각하고 있으며 교수님 및 학생들의 도움도 받는 정도로 나타났다. 아르바이트 하는 횟수는 2∼3일/1주로 나타났으며 용돈은 월 평균 30만 원 정도 쓰고 있는 것으로 나타났다. 한국 유학중 가장 어려운 문제는 언어적인 문제가 가장 높은 비중을 차지하였으며 다음은 경제적인 문제로 나타났다. 이런 언어적인 문제는 또한 한국친구들을 사귀지 못하는 원인으로 연결된다. 셋째, 문화적 영역에서 학생들은 한국의 영화나 드라마를 가장 즐겨보는 것으로 나타났으며 또한 한류에 대해 아는 정도도 비교적 높은 편이었다. 한국의 문화와 예의범절, 여가 문화 적응은 중간 정도로 나타났지만 한국의 명승고적에 대한 이해가 가장 낮은 것으로 나타났다. 결론적으로 중국유학생의 한국 대학생활 적응에 관한 연구를 통하여 현재 소속대학교에 재학 중인 외국인 유학생들의 실태를 언어적 측면, 생활적 측면, 문화적 측면으로 파악하는데 도움이 될 것으로 생각된다. 그러나 본 연구의 결과가 제한적인 조건에서 추진된 만큼 보다 많고 다양한 표본을 대상으로 하여 후속 연구가 수행됨으로써 연구결과의 일반화 가능성을 높일 필요가 있다고 본다. 외국인 학생들의 실태조사를 통하여 소속대학교는 세계화된 대학을 구축하기 위한 기반을 마련하고, 중국인 유학생들의 대학생활 적응에 관한 실태를 조사함으로써 그들이 한국에서 유학하는 동안 보다 잘 적응할 수 있는 계기를 마련해주어야 한다.

외국인 유학생의 한국 물류기업 취업의향과 영향 요인 연구 : 한경대학교 재학생을 중심으로

박준철 한경대학교 2018 국내석사

현장시험을 통한 연직배수재의 통수능 평가

이응기 韓京大學校 産業大學院 2005 국내석사

인공배수재를 타설하여 연약지반의 압밀을 촉진하는 연직배수공법에서 연직배수재의 통수능은 지반의 압밀특성에 영향을 미치는 것으로 알려져 있다. 일반적으로 현장에 타설된 배수재의 통수능은 압밀시간이 경과되면서 감소하는데 감소되는 이유로는 배수재의 배수단면적의 감소, 세립토사에 의한 배수재 휠터의 막힘(clogging)과 지반침하로 인한 타설된 배수재의 겹침(folding) 등의 변형으로 인한 것으로 알려져 있다. 준설토로 조성된 초연약지반의 지반개량에 인공배수재를 적용하는 경우 성토하중에 의해 발생되는 과도한 압밀침하는 배수재 겹침과 같은 변형을 유발시켜 통수능이 저하되어 압밀시간에 영향을 미치게 된다. Bergado(1996)는 각종 변형조건의 통수능 시험결과 통수능의 감소율은 배수재의 종류에 따라 차이가 크나, 일반적으로 10%굴곡변형에서 14~66%, 20%굴곡변형 에서 22~78%, 뒤틀린 경우에 25~85%의 통수능 감소를 보이는 것으로 실내시험결과를 발표한바 있으며, Holtz et al.(1991), Bergado(1996), 박(1994), 김(2001)등도 주로 실내실험을 수행하여 배수재의 변형에 따른 배수재의 통수능 저하를 평가하였다. 하지만 실제 연약지반에 타설된 배수재의 변형양상은 지반의 압밀속도나 침하크기 등에 따라서도 달라질 것이므로 배수재의 변형에 따른 통수능의 저하를 현실적으로 확인하기 위해서는 실제 현장에서의 통수능시험이 필요하다. 본 연구에서는 원지반 연약층 층후가 8m이며, 준설매립층후가 7m 인 총I5m 지반에 인공배수재를 타설하여 통수능의 변화를 조사하였다. 타설시 배수재 안쪽에 직경 6mm의 압력관을 설치하였으며, 지반침하에 의한 배수재의 겹침이 발생하더라도 물의 흐름에 영향이 없을 정도의 강성이 있는 재질을 사용하였다. 배수재를 타설하고 지반침하가 발생하지 않았을때 압력관을 통해 일정한 수압을 가하고 수압소산정도로부터 배수재의 통수능을 측정하고 지반의 압밀의 진행에 따른 통수능 저하를 측정하며 통수능 변화를 조사하였다. 시험결과 배수재 길이의 9% 침하발생이 통수능은 최고 95% 감소하였으며, 배수재 종류에 따른 통수능 저하 차이는 크지 않은 것으로 나타났다. PBD(Plastic Borad Drain) installed vertically by the mandrel accelerates consolidation of the soft ground, and consists of a flat plastic core wrapped with a filter sleeve non-woven geotextile filter. The relevant features for the design and performance of PBDs are their hydraulic properties: the discharge capacity of the cross-section, and the filter permeability which is known to decrease with time. Because the reason for reduction is not only minute soil particles of drain cross section are clogged in filter of drain board but also the deformation like folding of PBD. PBD was installed to improve the super soft soil dredged in the southern port of Korea. PBD foldings due to excessive consolidation settlement by it self-weight would be anticipated. Folding of PBD because of large settlements may result in a reduced discharge capacity. Bergado (1996) showed the big differences depending upon all sorts of results on discharge capacity and attrition rate of discharge capacity which was transformed by drain board altered 10% curve as 14~66%, 20% curve as 22~78%, and twist condition as 25~85%. which was estimated by Holtg et al(1991), Bergao (1996), Park(1994), and Kim(2001) with local experiment results. It was presented that the delay of consolidation would result in discharge capacity with deformation of PBD. However, a shape of deformation of PBD in soft ground relied on consolidation time and drain board features. It was performed that in-situ discharge capacity tests in construction field to verify decreased discharge capacity with time, with regarding to deformation rates of PBD. In the soft ground consisted of 8 meters thick of dreged soft soil underlying soft marine clay in the southern coastal area, total 15m depth, the PBDs were installed with a 6mm-diameter pressure pipe inserted in them. The pressure pipe is used with product enough stiffness without disturbance of water flow in the case of occurrence of folding which was created by ground settlement. It was pressured with a constant water pressure through pressure pipe, when there is no ground settlement, discharge capacity of PBD was contingent upon degree of water pressure dissipation. In the test results, it is investigated that discharge capacity approximately diminished 8% in occurring about 10% deformation. There might be little differences and lowered discharge capacity on kinds of PBDs.

수집 외대파의 생리적 특성과 품종간 유전적 다양성 분석에 관한 연구

손규형 韓京大學校 産業大學院 2002 국내석사

수집한 외대파의 특성을 비교하기 위하여 파종 후, 초장 연백장, 연백경, 생체중, 엽수, 화분의 유무 및 월동능력을 SAS통계프로그램으로 분산분석을 실시하여 통계적 유의성을 검토한 결과 조사항목에서 품종간 통계적 5%의 유의수준에서 유의한 차이를 보였다. 품종간 초장에서 품종간의 비교에서 가장 높았던 것은 동경화목(T.V. 토끼다사) 이었으며, 청용외대(F1, 다끼이)와 장죽한대파(F1, 동부한농) 순으로 나타났으며, 연백장에서 가장 높았던 동경흑동(TV, 도기다), 흑강외대파 (T.V, 세미니스)이었으며, 가장 낮은 수치를 보인 것은 금장외대파(TV, 동부한농)로 나타났다. 연백경의 경우 가장 높았던 품종은 한솔외대파(F1, 동부한농), 청용외대(F1 다끼이), 명성흑금장(F1, 동부한농)순으로 나타났으며, 조사항목 중 생산능력을 판가름할 수 있는 생체 중에서는 가장 높았던 흑금장(TV, 대농), 한솔외대(F1, 동부한농), 흑엽한대파(TV, 동부한농)순이었다. 수집품종간 유전적다양성을 평가하기위해 DNA flngerprinting을 이용하여 PCR분석을 실시한 결과 설난대파, 진성월동파, 동경하흑, 흑병장금에서 250bp의 특이적인 증폭산물(URP 02), 흑금장, 대관흑, 흑병금장 설장 한대파등에서 1,000bp의 부근의 특이적인 증폭산물(URP 03), 동경동흑, 청용외대의 품종에서 2,300bp부근에서 품종 특이적(URP05)으로 나타나 분자 marker로 품종을 구별 할 수 있었다. 또한 수집품종에서 얻어진 21개의 Polymorphic band를 이용하여 NTSYS에 의한 유전적 유사도에서 수집품종간 3 Group으로 나누어졌으며, 제1 Group에는 설난대파(F1, 동원), 진성월동파(F1, 동원), 동경화목(TV, 도기다) 흑병금장(TV, 사까다), 제2 Group에 분류된 동경동흑(TV, 도기다), 흑금장(TV, 대농), 설장한대파(F1, 세미니스), 청용외대(F1, 다끼이), 제3 Group내에서 청용외대 및 금장외대파를 제외하고는 2개의 소 Group로 나누어지는데 제1소 Group는 금장외대파(TV, 동부), 흑장외대파(TV, 신젠타), 한솔외대(F1, 동부), 명성흑금장(F1, 동부), 제2의 소 Group에는 흑강외대파(TV,세미니스), 흑협한대파(TV, 동부), 금장외대파(TV, 세미니스), 장죽한대파(TV, 동부)로 나눌수 있었다. 위 결과로 살펴볼 때 크게 두 가지 형태로 구분될 수 있는데 첫 번째는 일본 재래종을 근간으로 교배조합 및 선발을 통해 육성된 품종과 다른 하나는 우리나라 고유의 재래종을 기반으로 교배 및 선발을 통해 육성된 품종으로 명확히 입증할 수 있었다. In order to compare physiological characterization and genetic diversity among collected welsh onion(Allium fistulosum L.), 24 varieties was investigated plant height, blanching height, blanching stem, fresh weight, number of leaves, ability of hibernation and genetic diversity. These results obtained were summarized a s follow; 1 In the plant height, "Dongkyung Hwamoc (Tokida)" was showed the highest among varieties, followed Chungyong"(Dachn), " Jangjuk(Dongbu Hannong). 2. In the blanching height, "Dongkyung Dongheuk (Tokida)" was showed the highest among varieties, and "kumgang (Dongbu Hannong)" was showed the lowest. 3. In the blanching stem, the highest species is "Hansol( Dongbu Hannong)", followed "Chung Yong (Dachn)", "Myungsumg Heukkumjang (Dongbu Hannong). 4. In the fresh weight, which determine the ability of production, "Heuk Kum Jang (Daenong)" was showed the highest among varieties, followed Hansol (Dongbu Hannong)", "Heukhyup Han onion (Dongbu Hannong) 5. Penogram among 18 varieties generated by UPAGMA cluster analysis. The first group is Sulnan-dae-pa, Jinsung-woldong-pa, Dongkyung-hwa-moc, Heukbyung-kumjang. The second group is classified Dongkyung-dongheuk, Heukkumjang, Suljang-han-dae-pa, Chungyong-we-dae-pa. Especially the third group could be classify into sub-group of two exclusive of Chungyong-we-dae-pa and Kurnjang-we-dae-pa. The first sub-group has Kumjang-we-dae-pa, Heukjang-we-dae-pa, Hansol-we-dae-pa, Myungsung-Heukkum -jaw. The second sub-group has Heukkang-we-dae-pa, Heukkeup -han-dae-pa, Kumjang-we-dae-pa, Jangjuk-han-dae-pa. 6. DNA markers among collected varieties were showed unique band (URP02_(250bp)) in SulNan, Jinsung Woldong, Dongkyung Hwamoc and Heukmyum Kumjang. And URP03_(1000bp) was showed in Hgeuk Kumjang, Daekwan Heuk, Heukmyun Kumjang and Suljang Handaepa. Also, Dongkyung Dongheuk and Chungyong-we-dae-pa were showed with specific band URP05_(2300bp)

주문식 교육의 효율적 협력업체 발굴을 위한 사례 연구 : D대학을 중심으로

송장헌 한경대학교 전자정부대학원 2008 국내석사

Nowadays, most colleges have been faced with many difficulties, which had been caused the rapidly changing environment, in both internal and external. Internally, it has hard time inducing new students to be enrolled due to the lack of entrance resources. Furthermore, a number of investment is necessary in order to meet the students' various desire. Externally, on the other hand, reconstruction of an organization has occurred under keen competition among universities, the depression of economy makes the turnover of jobs decreased and keeps the unemployment rate of university graduate low and longer. The college is facing with the another problem under this crisis situation of the universities. Even though the goal of foundation of the college is to cultivate the worker who is professional, as what university does, it pursues both scholastic department of pedagogy and occupational training simultaneously.The way to survive from crisis for the 21st century colleges, however, is faithful to the foundation gist of the origin and develop the discriminated curriculum compared to universities's one. In this matter, the customized training system which is diffused rapidly in the college would be the one of alternatives. Also the government has increased the financial support every year for the active diffusion of customized training, and supported 28 billion won to 80 colleges in 2007. The customized training program is composed of the requests of the industries, and the experts and specialists who have the plenty of practical experience and professors participate together so as to develop a educational programs. Therefore, the first thing to be done is to seek industries which can be the demander and common provider in this system. The agreement between college and industries becomes the first step of all customized training programs. It has been started from the question how the college dig out industries efficiently under the present condition of the customized training program. While college share the role of customized training programs mutually, reaching to the agreement with industries, it is not easy for college to do the a thing. It means, college invests a plenty of resources like as university, however, the result is yet to be known. To increase the effect, invest the same amount of resources or save the resources to obtain the same effect. This research approached from the theoretical side that sees a collective agreement will be more efficient than the individual agreement. The effectiveness judgment has been set as standard with the person who is (the number of persons who are) demanded by the agreement enterprise. Based on this analyses, I analyzed the agreement enterprise of order training(student cultivation department of pedagogy and industries improvement training) of D college from 2004 until 2007. Then I divided the type of agreement enterprise with the group and individual case, and compared and analyzed with the result of D college. From the analysis of examples in D college, the four implications have been deduced. First, the collective agreement is more efficient than the individual agreement on a contract agreement of customized training. Second, the group collective agreement is preponderated in special subject and process only. Third, continuity of agreement is not enough as compared to the importance of group collective agreement. Fourth, the connection between agreement enterprise and other department of college is insufficient. In conclusion, what is the most essential for the college to achieve success of customized training is that to make an more effort on the collective agreement contract than Individual agreement. In addition, to raise the effectiveness and the benefit shall be needed from the active agreement contract exchange.

ACP-PCR 기법을 이용한 복제 수송아지 폐 조직 특이발현유전자 발굴

김창회 韓京大學校 生物環境·情報通信專門大學院 2007 국내석사

본 연구는 생후 6개월째에 폐 관련 질환으로 사망한 체세포 복제 수송아지 한경 100호의 병리학적 사망원인인 소합포성 폐렴과 아카노박테리아성 폐렴이 진단되어 직접적인 폐사의 사인이 호흡기질환 이외에 유전적인 부분에서의 원인을 분석하기 위하여 실시하였다. 복제 수송아지 한경 100호의 폐와 동일한 연령의 정상 수송아지의 폐를 이용하여 두 조직 간에 다르게 발현하는 유전자 (differentially expressed gene, DEG)를 발굴하고 그 유전자의 특성을 확인하기 위하여 annealing control primer (ACP)-PCR, cloning, sequencing, quantitative real-time PCR 및 semi-quantitative RT-PCR을 실시하였다. 본 연구를 통하여 얻은 결과는 아래와 같다. 1. Annealing control primer-PCR 기법을 이용하여 복제 및 정상 수송아지의 폐에서 총 46개의 DEG를 발굴하였다. 2. 발굴된 46개의 DEG들 중에서 12개의 복제소 특이발현 유전자를 확인하여 각 유전자의 특성을 확인하고자 염기서열 분석을 실시하였다. 3. 염기서열 분석을 통하여 유전자의 특성을 확인한 결과 복제 수송아지 한경 100호가 한우임이 확인되었으며, 또한 소의 구강, 폐 또는 비강 상피암 (palate, lung, and nasal epithelium carcinoma associated (PLUNC) 관련 유전자를 발굴하여 한경 100호의 사망원인이 감염성 폐렴 이외에 유전자 발현 이상에 따른 폐의 기능 이상일 가능성을 확인하였다. 4. 소 구강, 폐 또는 비강 상피암 관련 유전자를 정량 분석한 결과 복제 수송아지 폐 조직에서만 특이적으로 발현하는 것을 확인하였다. 이상의 연구 결과를 살펴보면 생후 6개월째에 감염성 폐질환으로 사망한 한경 100호의 경우 앞에서 언급한 병리학적 소견 이외에도 PLUNC 유전자의 과 발현에 의한 폐 기능의 이상이 사망원인의 중요한 한 요인이 될 수 있었음을 확인하였으며, 이 유전자의 유전적 특성에 대한 추가적인 연구가 이루어질 경우 복제동물 생산에 유용한 자료가 될 것으로 사료된다. The cloned Hanwoo male calf of Hankyong #100 was died by the result of bovine respiratory syncytial virus (BRSV) and arcanobacterial pneumonia at 6-month old. This study was performed to determine the causes of the clone's death in genetical aspects in addition to the pathological diagnosis. To identify and analyze the characteristics of the differentially expressed genes (DEGs) in cloned or normal lung tissue, annealing control primer (ACP)-PCR, cloning, sequencing, quantitative real-time PCR, and semi-quantitative RT-PCR were performed. The results obtained from this study were as follows: 1. Total 46 DEGs were identified from lung tissues of both calves using ACP-PCR. 2. Among 46 DEGs, 12 DEGs were expressed specifically in Hankyong #100. These DEGs were identified, cloned and sequenced to analyze the characteristics. 3. According to sequencing and BLAST search, it was confirmed that the clone was Hanwoo and the gene of palate, lung, and nasal epithelium carcinoma associated (PLUNC, NM_174426) was overexpressed in lung tissue of Hankyong #100. 4. We confirmed that the gene identified was expressed specifically in lung tissue of Hankyong #100 using semi-quantitative RT-PCR Based on these results, it can be postulated that Hankyong #100 may be died by the disfunction of lung associated with the overexpression of the PLUNC gene as well as a infection of BRSV and arcanobacterial pneumonia. Further studies are needed to understand the functions and characteristics of this gene in cloned animals.

專門大學生의 中途脫落 要因 分析에 關한 硏究 : 동원대학을 중심으로

손창수 한경대학교 2009 국내석사

The aim of this study is to analyze the factors influencing the college students' dropping out of the college. This study provides an analysis of the college dropouts in recent four years and a preventive measure for them. As a result of this study, 1st year male students compared with 1st year female students and 2nd year students tend to give up their studies easily. It is also found that the students on leave of absence tend to eventually drop out. It is also found that withdrawal from school occurs mostly in March. And it is found that the students who got a scholastic warning tend to leave the school more easily than the students who didn't. The students who got scholastic warning tend to leave the school voluntarily or get expelled from the school without enrolling. It is found that the students who took the leave of absence due to the poor scholastic achievement tend not to return to the school. Therefore, we may consider the students with poor scholastic achievement, like the students who took the leave of absence, as would-be dropouts. In the analysis of the reasons for leaving the school, it is found that the dissatisfaction with their majors or departments usually makes them to cram to repeat entrance exams, to enter other colleges, or to act badly in studying majors. The dissatisfaction tend to be greater reasons for withdrawal from the school than home environment, personal reasons, or economic reasons. It may be concluded that we should try to bend our efforts in guiding the freshmen with concern and counselling, finding measures in preventing long-term absences, and developing new programs helping the students to continue their studies rather than taking the leave of absence in order to reduce the number of dropouts.

한우에서 CIDR와 bST가 수정란이식 효율과 유전자 발현에 미치는 영향

이호준 韓京大學校 生物·情報通信專門大學院 2005 국내박사

This study was conducted to the effect of CIDR and/or bST treatment on the production of in vivo embryos and pregnancy, on the concentration of plasma hormonal levels and on the expression of genes associated with reproduction in blastocysts and uterine epithelial cells in Hanwoo. The results were as follows; 1. The number of embryos collected and transferable on CIDR or CIDR+bST treatment was 9.5±1.9 or 13.1±1.1 and 6.9±1.6 or 9.6±1.8, respectively. The grade 1 embryos in CIDR+bST group(84.4%) was significantly higher than that in CIDR group(60.6%) (p<0.001). However, the grade 2 embryos in CIDR+bST treatment(8.9%) was detrimentally lower than that in CIDR treatment(27.9%) (p<0.05). 2. The pregnancy in recipients treated with bST(68.6%) was significantly higher than that in recipient without bST treatment(39.1%) ((p<0.05). The transferred embryos were recovered from the donors without bST treatment. However, the pregnancy in the bST treated recipients(69.0%) was similar to that in no bST treatment group(63.0%). The transferred embryos were recovered from the donors with bST treatment. 3. The concentration of plasma P4 in CIDR+bST group(14.9±5.5ng/㎖) tended to higher than that in CIDR group(6.4±8.4ng/㎖), although the difference was not statistically significant. The plasma IGF-Ⅰ level in CIDR+bST group (1691.4±195.3ng/㎖), however, was significantly higher compared to that in CIDR group(576.1±289.7 ng/㎖) (P<0.01). 4. The size of corpus luteum did not differ regardless of bST treatment. The concentration of plasma P4 in recipients at day-7, day-14 and day-21 of estrus cycle was not different in either with or without bST treatment. But the plasma IGF-Ⅰ level in bST treatment group(891.3±78.8ng/ml) at day-7 of estrus cycle in recipients was significantly higher than that in the group without bST treatment(398.5±93.3ng/ml) (p<0.01). 5. Plasma P4 level showed positive correlation with plasma IGF-Ⅰ level in bST treatment group, however, the concentration of plasma P4 showed negative correlation with the concentration of plasma IGF-Ⅰ in recipients without bST treatment. 6. In bST treated recipients, there was a positive correlation between day-7 and d-14 plasma P4 level. They showed increasing tendency continuously at those days. However, there was a negative correlation between day-7 and day-14 plasma level in the recipients without bST treatment. The concentration of plasma P4 at day-14 showed decreasing tendency after day-7(r=0.55, P=0.09). 7. There was a positive correlation between plasma P4 level and the size of corpus luteum at day-7 in both with and without bST treatment(r=0.57, p<0.05) in recipients. The bigger size of corpus luteum, the higher concentration of plasma P4 in both groups. 8. There was a positive correlation between plasma P4 level and the size of corpus luteum at day-14 in bST treatment. However, the concentration of plasma P4 was decreased at day-14 of estrus cycle in recipients without bST treatment, although the size of corpus luteum was increased at that day(r=0.53, p=0.06). 9. It was found 584bp of specific band(B217) in epithelial cells in the recipients treated with bST using RAPD-PCR analysis. After analysis amino acid, it had 94aa of open reading frame(ORF). It had 10 serine and 10 tyrosine residues. Isoelectric point(pI) was 7.3 and hydrophobicity was -0.36. It could be postulated that it is soluble protein. 10. To analyze the expression pattern of B217 gene in different cell types, PCR was performed. There were bands in day-14 of fresh and in vitro cultured uterine epithelial cells, liver and in vitro cultured fetal fibroblast cells, however, there were no bands in cumulus and granulosa cells and serum starved fetal fibroblast cells. 11. The expression of interferon tau, COX-2, oxytocin receptor, progesterone receptor, and estrogen receptor was strong in day-14 of embryos produced by IVF and day-14 of uterine epithelial cells in donors without bST treatment, day-14 of embryos produced by IVF, day-14 of uterine epithelial cells in donors without bST treatment, day-7 of uterine epithelial cells and day-14 of embryos produced by IVF and day-7 of uterine epithelial cells in donors treated with bST, respectively. 12. Somatotropin receptor was only expressed in day-14 of embryos produced by IVF and day-14 of uterine epithelial cells in donors with bST treatment. The expression of growth hormone receptor and pregnancy associated plasma protein-A was strong in day-14 of uterine epithelial cells in donors with bST treatment. Based on these results, it could be concluded that the treatment of CIDR+bST during superovulation procedures was an effective method to produce high quality and quantity of in vivo embryos and to obtain higher pregnancy rate caused by the maintenance of endocrine regulation. And we found B217 gene expressed specifically in day-7 of uterine epithelial cells in donors with bST treatment, however. the characteristics and genetic informations of this gene were not evaluated yet. The further studies of this gene are needed to understand the mechanisms associated with implantation and pregnancy.

WAP 환경에서의 학사정보지원시스템 개발

노영희 한경대학교 생물정보통신전문대학원 2006 국내석사

모바일 통신은 급속도로 성장하고 있고 20년 이상동안 활발한 연구가 진행되어있다. 그 결과의 예로 모바일 전화 사용자를 위한 서비스 등 매우 다양한 분야에서 사용되고 있다. 오늘날 모바일 전화 사용자는 언제, 어디서나 서비스들을 제공받을 수 있다. 다양한 모바일 무선 인터넷 서비스는 모바일 통신과 인터넷 기술의 통합에 의해 발전되어 왔다. 2005년 7월 현재, 국내 이동 전화 가입자 수는 총 3,768만 명으로 전체 인구의 80%에 해당한다. 그 중 90%가 넘는 가입자들은 모바일 무선 인터넷을 사용하고 있다. 유선 인터넷 서비스를 제공 받는 사용자는 시간과 장소에 제약을 받는다. 현재 모바일 무선 인터넷 시스템은 어떠한 제약없이 이 서비스를 모든 사람들에게 제공할 수 있다. 특히, 일반 사용자인 학생들은 수강신청, 성적조회, 강의실 조회 등과 같은 모바일 서비스 사용을 매우 필요로 한다. 본 논문에서는 WAP 환경에서의 학사정보지원시스템을 제안한다. 학사정보는 캠퍼스에서 학생들에 의해 활발히 사용되고 있다. 따라서 본 논문에서는 시간과 장소뿐만 아니라 유선 인터넷 서비스에 제한없이 학생들이 쉽게 서비스를 이용할 수 있도록 하기 위해 이 시스템을 설계, 구현하였다. 향후 다양한 학사정보를 제공하기 위해 더 많은 서비스가 개발되어야 할 것이다. 본 논문에서 제안된 시스템의 결과는 캠퍼스 학생 중에서 LG-텔레콤과 SK-텔레콤 가입자에게 유용하다는 것을 입증한다. Mobile communication is growing rapidly, and has now been an active area of research for over two decades. Their results, example, services for mobile phone users, are being used in many different areas. Today, mobile phone users are able to be provided these services at anytime, anywhere. Diverse mobile wireless internet services have been developing by the integration of mobile communication and internet techniques. As of July 2005, subscribers of mobile phone was 37,680 thousands in South Korea, which corresponds to 80% of our total population. The 90% of these subscribers uses the mobile wireless internet services. Users supported by wire internet service have been restricted to time and places. Now, mobile wireless internet systems can provide all people with these services without any limitations. In particular, students of generic users need to use widely these mobile services such as registering classes, referencing their grades, finding classrooms, and so on. In this thesis, we propose a School Affairs Information Support System in a WAP environment. The school affairs information is actively being used by students at campus. Thus we design and implement our system so that students use easily these services without being restricted to time and places, as well as existing wire internet services. In the near future, more these services will have to be developed in order to provide various school affairs information. The results of the proposed system validate that our system is useful to campus students, including subscribers of both LG-Telecom and SK-Telecom.

돼지의 CART와 TNF-α 유전자내 다형성 관찰 및 경제형질과의 연관성 분석

이제현 한경대학교 생물환경·정보통신전문대학원 2008 국내석사

CART(Cocain-and amphetamine-regulated transcript) peptides are neurotransmitters that have receives much attention as mediators of feeding behavior and body weight regulation in mammals. TNF-α (Tumor necrosis factor-α) production by adipocytes could be involved in the pathogenesis of insulin resistance. The objectives of this study were to detect associations of genetic variations with economic traits in the porcine CART and TNF-α gene as candidate genes for growth and body weight traits in pigs. In this study, SNPs were screened in CART(Accession no. EF581838) between intron 1 to 3'UTR from 191 independent pig by DNA sequencing and PCR-RFLP. Total 9 SNPs were found and C+1543T SNP(ATC:Isoleucine → ATT:Isoleucine) of them detected in coding region. But economic traits(body weight, average daily gain, backfat thickness, age at 90kg) were not significant(p>0.05) in any model. Also, the SNPs were screened in TNF-α(Accession no. AJ251914) between exon 1 to exon 4 from 191 independent pig by DNA sequencing and PCR-RFLP. Total 3 SNPs were found and G+4648A(GGC:Glycine → AGC:Serine), C+4894T(CTG:Leucine → TTG:Leucine) SNPs of them detected in coding region. The frequency of two SNPs was G+4648A(0.51/0.49) following C+4894T(0.05/0.95). G+4648A SNP had a significant effect(p<0.05) on body weight. C+4894T SNP had a significant effect on average daily gain(p<0.01) and age at 90kg(p<0.05). The results obtained from using candidate genes may be used as an useful index for the genetic improvement of body weight and growth in pig.