水稻作에 對한 珪酸質肥料의 效果

이종호 東亞大學校 1964 국내석사

濟州島の植生に關する植物社會學的硏究 : 제주도 식생에관한 식물 사회학적 연구

김문홍 濟州大學校 2000 해외박사

Atonik의 藥劑處理(葉面撒布)가 고추의 生長에 미치는 影響

하상영 東亞大學校 大學院 1966 국내석사

水稻 新育成品種의 裁培時期 移動에 따른 物質生産과 生長解析에 關한 硏究

이승필 경북대학교 대학원 1977 국내석사

Orchardgrass 및 ladino clover 牧草地에 있어서 牧草와 바랭이 (Digitaria sanguinalis〔L.〕Scop.) 間의 競合에 關한 硏究

김창주 서울大學校 大學院 1974 국내박사

Cucumis sativus의 葯培養에 關한 硏究 : (1) 2倍性 Callus의 組織學的 觀察((1)Histclogical studies on the diploidal callus)

朴鶴封 전북대학교 1975 국내석사

The Histological studies were carried out about the diploidal callus appeared on the 2 kinds of the media used in the anther culture of the cucumber. The results obtained were as follows : 1. The calli were appeared on the all sides of the anther wall tissue. 2. After about 7 days culture, the calli were appeared as a root hair-like or a stick-like root, and consisted of light yellow. 3. The meristematic tissues of the callus were located inside the surface of the callus. 4. The liquid calli were appeared on the medium M_1 supplemented 2, 4-D. 5. The tracheid-like structure were formed on both of the media, M_1 and M_2. 6. After about 30 days culture, the root-primordia were differentiated from the meristematic tissue of the callus on the medium M_2 and grew into the root in a few days.

全北地方에 있어서의 사탕무우 適應性 檢定試驗

崔京求 全北大學校 大學院 1973 국내석사

In order to investigate the adaptability of sugar beets and their major agronomic characteristics in Jeonpuk area, this experiment was conducted with 7 varieties used, such as Maribo poly and the other 6 varieties, at Jeonju (51^m altitude) and Muju (650^m altituce), the results being summerized as follows ; 1. In juvenile stage, the growing state of sugar beets at Jeonju plots is better then that of those, at junju. But, their states iv. the maturing stage, are rather favorable at Muju than at Jeonju. 2. The bumper growing state comes in the late of July at Jeonju and in the middle of August at 1 Muju. 3. At Jeonju, the Cercospora leaf spot disease in sugar beets begins to appear in the middle of July and its outbreak reaches at the maximum in the middle of August. At Muju, it breaks out in the middle of August, but its degree is considerably slight. Kawecerco poly, a sugar beet variety, showes the highest resistance to the Cercospora leaf spot disease among the varieties examined at Jeonju and Muju. 4. T/R ratio at the harvest time is 0.08-0.13 at Jeonju and 0.42-0.77 at Muju. Therefore their ratios at Muju are outstandingly higher than those at, Jeonju. 5. Total sugar contents (%) of the varieties at Muju are generally higher than those at Jeonju. In comparison, Kawemega p. that showes the highest content among them has 14.9% content, at Jeonju, and at Muju, Kawecerco p. and Kawesaccha p. showes above 20.0% and 20.1%. respectively. 6. Root yields per 10 acre at Jeonju are significantly higher, in comparison with the yields at Muju. Maribopoly, the variety of the highest root yield at Jeonju, produces 5,572 kg, and Kawemega p. at Muju, 3,670 kg. 7. The varieties of the highest sucrose product are kawemega poly, 672.3 kg per 10 acre, at Jeonju, and Kawecerco poly, 632.9 kg at Muju. 8. In these respects, it is considered that the most favorable sugar beet variety is Kawecerco poly in this experiment.

過濕에 의한 콩의 生長과 生理的 形質의 反應

이재은 忠北大學校 2005 국내박사

在來種 들깨의 作物學的 特性과 分類

홍성택 忠北大學校 2004 국내박사

Bromelain 및 cathelicidin 유전자 도입에 의한 배추의 내병성 증진 효과

정유진 忠南大學校 2007 국내박사

The main purpose of this study is to investigate the function of bromelain gene from pineapple and cathelicidin peptide originated from human on enhancing resistance through raising transgenic cabbages, testing gene expression and evaluating resistance to diseases. The experimental results are summarized as follows; Chapter 1: Expression of a BAA1 gene encoding fruit bromelain in Chinese cabbage enhances resistance to soft rot disease 1. BAA1 gene was isolated from pineapple, build Ti-plasmid vector under control of CaMV 35S promoter in pIG121Hm, and transformed into Agrobacterium tumefacienciens strain LBA 4404. Eight whole plants were regenerated after transformation of cotyledon of Chinese cabbage. The eight transgenic plants were used to bring progenies. 2. Presence of BAA1 gene in the eight transgenic plants was confirmed by PCR analysis and expression of the gene in the plant was also confirmed by RT-PCR analysis. For the exact dose of mRNA, real-time PCR was held and the amount differed between the transgenic plants. The most highly expressed transgenic line was B66-3-1. 3. BAA1 protein was found at 120kDa of the transgenic plant by Western blot analysis, and B66-3-1 line had 0.9% more total protein compared to wild type plants in ELISA analysis. 4. B66-3-1 line harboring the new BAA1 gene showed difference on expression of 2,013 genes in microarray analysis. Among those, over-expressed genes were defense related protein (At1g75830), protein kinase (At2g40560), and protease inhibitor (At5g38195) which were over eight times more expressed compared to non-transgenic plants. 5. To find out the resistance to the soft rot of transgenic cabbage which BAA1 gene was expressed, leaves of the transgenic lines were inoculated with Pectobacterium carotovorum ssp. carotovorum at the density of 2 × 10^(4) and 2 × 10^(6) CFU/ml. Although there was difference in symptom development between the transgenic lines, scarcely no soft rot symptoms developed at 2 × 10^(4) CFU/ml and a little disease developed at 2 × 10^(6) CFU/ml in the transgenic plants. But soft rot symptom was found in 24 hours in the wild type plant even at 2 × 10^(4) CFU/ml. Chapter 2: Expression of a antimicrobial peptide gene(Cathelicidin LL-37) in Chinese cabbage enhances resistance to both fungal and bacterial pathogens 1. Cathelicidine LL-37 peptide gene was amplified by PCR, and constructed at E. coli expression vector pET 28. As the result of examining the antifungal ability to gram negative E. coli DH5α and gram positive Bacillus subtilis, the clear inhibition zone was found. 2. Plant infection vector VB2 with LL-37 gene was constructed, transformed into Agrobacterium tumefacienciens LBA 4404 line, infected the cotyledon of cabbage and raised 20 transgenic plants. Presence of HPT, 35S promoter, and LL-37 genes in four transgenic plants were confirmed by PCR analysis. 3. LL-37 mRNA was detected in four transgenic plants by RT-PCR analysis and the expected 4kDa sized protein band was found in the four transgenic plants by Western blot analysis. 4. To find out the resistance to the soft rot of transgenic cabbage which LL-37 gene is expressed, leaves of transgenic lines were inoculated with Pectobacterium carotovorum ssp. carotovorum at the density of 2 × 10^(4) and 2 × 10^(6) CFU/ml. After 12 hours, tissues of the leaves of the wild type plant began to soften and soft rot symptoms were found all over the leaves after 48 hours. However, in transgenic lines except line B23, there was no soft rot symptom developed even until 72 hours after inoculation. 5. Transgenic plant harboring LL-37 gene showed 66%, 75%, and 80% more resistance to Colletotrichum higginsianum, Rhizoctonia solani and Fusarium oxysporium, respectively than wild type plant. 본 연구에서는 파인애플 유래 bromelain 및 인체 유래 cathelicidin 유전자를 이용하여 형질전환 배추 육성, 유전자의 발현 및 식물병원균에 대한 저항성 증진효과를 조사하여 얻어진 결과를 요약하면 다음과 같다. 제1장: 파인애플 유래 Bromelain 유전자 (BAA1) 도입에 의한 배추의 무름병 저항성 증진 1. 파인애플로부터 BAA1 유전자를 분리하여 pIG121Hm vector에 CaMV 35S promoter로 조절되도록 Ti-plasmid vector를 구축하고, Agrobacterium tumefacienciens LBA 4404 계통에 형질전환 하였다. 이를 매개로 배추의 배축에 감염하여 얻어진 형질전환식물체 중 8개체가 완전한 식물체로 재 분화 되어 후대를 육성하였다. 2. 형질전환 식물체에서 도입유전자의 확인을 위한 PCR 분석 결과 조사한 8개의 형질전환 식물체에서 예상하였던 band가 검출되었고, RT-PCR 분석 결과 BAA1 유전자가 발현되고 있음을 확인하였다. 또한 mRNA의 정량을 위해 real-time PCR을 수행한 결과 형질전환 식물체들에서 발현 양의 차이를 보였고, 가장 높게 발현하는 형질전환 계통은 B66-3-1이었다. 3. Western blot 분석 결과 형질전환 식물체의 120kDa 부근에서 BAA1 단백질의 발현을 확인하였고, ELISA 분석을 실시한 결과 B66-3-1 계통은 대조식물체보다 총 단백질에서 0.9% 정도로 높게 발현하였다. 4. BAA1 유전자가 도입되어 발현하고 있는 B66-3-1 계통은 microarray 분석 결과 총 2,013개의 유전자에서 발현량의 차이를 보였다. 그 중 over expression 되고 있는 유전자는 식물 방어관련 단백질 (At1g75830), protein kinase (At2g40560) 및 protease inhibitor (At5g38195)로 대조 식물체에 비해 발현양이 8배 이상 많았다. 5. BAA1 유전자가 발현하는 형질전환 배추의 무름병 저항성 정도를 조사하기 위해 형질전환계통들의 잎에 직접 2 × 10^(4)과 2 × 10^(6) CFU/ml 농도로 감염시켜 24시간 후의 발병률을 살펴본 결과 형질전환 계통들 간에 차이는 있었으나, 2 × 10^(4) CFU/ml에서는 무름 증상이 거의 나타나지 않았고 2 × 10^(6) CFU/ml에서는 약간의 무름 증상을 보였다. 그러나 대조 식물체에서는 2 × 10^(4) CFU/ml에서도 24시간 내에 모두 무름 증상이 나타났다. 제2장: 인체 유래 cathelicidin 유전자 (LL-37) 도입에 의한 배추의 항균력 증진 효과 1. 인체 유래 cathelicidin 유전자 (LL-37)는 PCR 분석에 의해 유전자를 분리하고 E. coli 발현 vector pET 28에 구축하여 그람 음성균 E. coli DH5α와 그람 양성균 Bacillus subtilis의 항균활성여부를 검토한 결과 생장이 억제되는 clear zone을 볼 수 있었다. 2. LL-37 유전자는 식물감염벡터 VB2에 구축하여 Agrobacterium tumefacienciens LBA 4404 계통에 형질전환 하였고, 배추의 배축에 감염하여 20개의 형질전환체를 육성하였다. 그리고 PCR 분석을 통해 HPT, 35S promoter 및 LL-37 유전자의 도입여부를 확인한 결과 4개의 형질전환 식물체에서 예상한 band를 검출하였다. 3. 형질전환 식물체들의 발현 양상을 알아보기 위해 RT-PCR 분석한 결과 4개의 형질전환 식물체에서 LL-37 mRNA가 검출 되었고, Western blot 분석을 통해 4개의 형질전환 식물체에서 4kDa 크기의 band를 검출 하였다. 4. LL-37 유전자가 발현하는 형질전환 식물체들의 무름병 저항성 정도를 조사하기 위해 형질전환계통들의 잎에 직접 2 × 10^(4) 과 2 × 10^(6) CFU/ml 농도로 감염시킨 결과 대조식물체에서는 감염 후 12시간부터 조직이 무르기 시작하여 48시간 후에는 잎 전체에 무름 증상이 나타났다. 그러나 형질전환 계통에서는 B23계통을 제외하고 감염 후 72시간 까지도 무름 증상이 나타나지 않았다. 5. LL-37 유전자가 발현되는 형질전환체는 대조식물체에 비해 탄저병균(C. higginsianum), 밑둥썩음병균(R. solani) 및 시들음병균 (F. oxysporium)에 대한 저항성이 각각 66%, 75% 및 80% 증진된 효과를 보였다.