수입산 식물성 생약제 원료의 중금속 함량 및 안전성 평가

안효진 부경대학교 2023 국내석사

Interest and demand for herbal medicines with a wide range or uses due to various pharmacological actions are increasing. However, as domestic production decreases due to the aging of rural areas, it is dependent on imports, and imported herbal medicines may cause heavy metal contamination problems depending on the medicinal part and the place of production. As the need to evaluate the safety of heavy metals in such illegally distributed herbal medicines is emphasized, this study evaluated the heavy metal content and safety of imported herbal herbal medicines. Lead, cadmium, and arsenic were analyzed in order to conduct heavy metal analysis on 935 cases of 29 herbal medicines imported and distributed in Busan for three years from 2019. The microwave digestion method was used to determine the concentrations of lead, cadmium, and arsenic in samples by using ICP-MS (NexlON 350, Perkin Elmer, USA). In addition, the mercury content was detected by using a mercury analyzer (IT/DMA-80, Milestone, USA). Lead, cadmium, arsenic, and mercury were detected below the standard values, and the content of lead and cadmium was higher in the above-ground part than in the underground part, but in the case of arsenic, the underground part was higher than the above-ground part. When heavy metal content was compared by country of origin, lead and cadmium were detected the highest in Vietnam, and arsenic was highest in Russia. In the case of mercury, there were no significant differences by country. Higher levels of lead, cadmium and arsenic were detected in China and Vietnam, which are among the top importing countries of herbal medicines, compared to other countries. When heavy metal content was compared by year, no heavy metal exceeded the standard, but the number of cases with lead content of 4 mg/kg or more occurred once every year for 3 years, and the number of cases higher than the average cadmium limit of 0.3 mg/kg was in 2019 33 Case, 26 cases in 2020, 22 cases in 2021. As a result of analyzing the heavy metal content and evaluating the risk of imported botanical herbal medicine raw materials, there were no samples exceeding the standard for heavy metal content, and almost all samples were analyzed to be safe. However, Glycyrrhizae Radix, Angelicae Gigantis Radix, Cinnamomi Cortex, and Ulmi Cortex, where relatively high levels of heavy metals were detected in lead, cadmium, and arsenic, showed higher detection values than other samples, and in Vietnam and China, which are high-income countries, higher levels than the overall average by country. Therefore, continuous monitoring of herbal medicine products, particularly concerning heavy metals, should be adopted to ensure human safety and security.

LC/MS/MS, GC/MS/MS를 이용한 국내유통 다소비 농산물의 잔류농약에 대한 안전성 평가

박영주 부경대학교 2023 국내석사

In this study, we conducted a survey for the safety management of 511 agricultural chemicals and verified the multicomponent analysis method. Brown rice and citrus were selected from the representative agricultural products, and 511 pesticides were analyzed by using Quick, Easy, Cheap, Effective, Rugged and Safe EN method and LC/MS/MS and GC/ㅌMS/MS. In order to verify the effectiveness of the multicomponent analysis method, a calibration curve was prepared at 6 concentrations in the range of 0.005-0.2 mg/kg containing 0.01 mg/kg of PLS level using an untreated sample solution. In all specimens, the correlation coefficient of pesticides was (R2) 0.8 or higher, showing high linearity, and the quantitative limit of each component was 0.01 or less. As a result of verifying the recovery rate at the LOQ level in the sample, it was confirmed that the recovery rate was 60-120% for most pesticides, and the relative quasi-deviation was less than 31%. In this study, as a result of verification, the multi-component analysis method confirmed the applicability of a survey on residual pesticides among agricultural products. In addition, a total of 511 pesticides (577 ingredients) were analyzed using GC/MS/MS and LC/MS/MS for 280 agricultural products of 15 kinds, and 159 pesticides of 95 kinds were detected from 12 kinds, all below the acceptable residual standard.

다당류 및 TGase를 처리한 동결 무지개송어육을 이용한 간편식 가공기술 개발

박혜민 부경대학교 2023 국내석사

국내산 마른김의 물리화학적 및 관능적 품질지표를 이용한 품질등급체계 개발

이우진 부경대학교 대학원 2023 국내석사

국내 혼획 고래류의 섭취기준 마련을위한 무기질 및 중금속 함량 평가

한형구 부경대학교 2023 국내석사

Effects of Physical Structure and Surface Charge of Adsorbents for Reduction of Biogenic Amines in Salt-Fermented Fish Sauce

Jung Jin In 부경대학교 대학원 2023 국내석사

3D 푸드 프린팅을 위한 어류 단백질의 영양강화 및 물성개선 기술개발

안병규 부경대학교 대학원 2023 국내석사

멸치액젓잔사를 활용한 조미소재 및 수용성 칼슘 제조기술 개발

손승아 국립부경대학교 대학원 2024 국내석사

For the acid-hydrolyzed fish sauce produced using the optimal conditions derived from preliminary research, a quality evaluation was conducted using fish sauce, seasoned fish sauce, and acid-hydrolyzed soy sauce as control with the similar raw materials and manufacturing methods. It has been confirmed that the ingredients vary depending on whether the fish sauce by-product is reused by the company. First, the contents of amino acid nitrogen was analyzed as 443.80 ± 5.44 mg/100 g for not reused fish sauce by-product whether it was 257.96 ± 5.11 mg/100 g for reused fish sauce by-product. The amino acid nitrogen contents of the not reused fish sauce by-product was at a level that met the standards for anchovy seasoned fish sauce in the Korean Industrial Standard, and in the case of free amino acids, the free amino acids that affected taste were at the level of (82.70%-82.83%) of the total free amino acids. It could be anticipated that it might be used as a rice polishing ingredient. The Content of histamine, the most important and problematic biogenic amine, was found to be 2.16 ± 0.10 mg/kg for unreused fish sauce by-product and 149.34 ± 5.71 mg/kg for reused fish sauce by-product. Both the not reused fish sauce by-product and the acid-decomposed fish sauce made from the reused fish sauce by-product did not exceed the histamine contents and were found to meet the standard. Neither unreused nor reused fish sauce by-product was detected 3-MCPD. Therefore, it was found that it did not exceed 0.02 mg/kg, which is the standard for 3-MCPD for acid-decomposed soy sauce, and the safety of acid-decomposed fish sauce was confirmed. Lastly, in the case of minerals, the significantly checked item was Ca. The contents of Ca in not reused fish sauce by-product was 519.15±41.96 mg/kg, and the reused fish sauce by-product was 806.59±21.75 mg/kg. It was confirmed that the Ca of acid-decomposed fish sauce manufactured using fish sauce by-product was at least 2.5 to as much as 4 times higher. Therefore, it is possible to prepare rice polishing ingredients with enhanced Ca, and it is believed that this may have a special advantage of acid-decomposed fish sauce using fish sauce by-product. Thus, it is judged that the rice polishing ingredients extracted from each fish sauce by-product will have commercial value in itself if standards and specifications are established. It is believed that it can be used as an additional ingredient to overcome the limitations of price and cost. In addition, as the fish sauce by-product is decomposed with acid, the calcium contained in the by-product is dissolved and precipitated again as calcium phosphate upon neutralization. The precipitated calcium phosphate is affected by pH. Thus, the supernatant of the fish sauce by-product was used as a seasoning materials ingredient, and the precipitate was developed into a calcium phosphate material to study ways to fully utilize the fish sauce by-product. The precipitated calcium phosphate was washed with water to reduce the high salt concentration, and was incinerated in a furnace to remove organic matter and developed into calcium material. At this time, 800℃ and 1,000℃ were compared and analyzed to derive the appropriate temperature. Through mineral analysis, it was found that washing with water increased the purity of phosphorus and calcium, however decrese sodium. It was found that temperature did not significantly affect the mineral contents when washing with water. However, in X-ray diffraction analysis, it was found that as the temperature increased, crystallinity increased and the purity of the crystals increased, and it was found that incineration at 1,000℃ was appropriate. As a result of X-ray diffraction analysis, the crystal was analyzed to be Whitlockite, a form of Mg combined with calcium phosphate, and some Trimagnesium bis(phosphate(V))-Ⅲ was also found to be present. Through SEM and STEM analysis, it was analyzed that the majority of Ca and P were distributed in the elemental composition, and the particle size was analyzed to be around 10 ㎛. Therefore, it was possible to develop calcium phosphate material simply by incinerating the sediment. To increase its absorption rate, it was attempted to manufacture water-soluble calcium. Including chelating calcium with an organic acid, an attempt was made to dissolve calcium by lowering its pH using vitamin C, however only 0.22% was dissolved at the maximum. So, additional research is believed to be necessary to prepare water-soluble calcium material.