퇴행성관절염의 사람 무릎관절 윤활막에서 Tenascin 발현에 대한 면역전자현미경적 연구

임형수,최희준,이세정,황덕호,Lim, Hyoung-Soo,Choi, Hee-Joon,Lee, Se-Jeong,Hwang, Douk-Ho 한국현미경학회 2005 Applied microscopy Vol.35 No.2

사람 무릎관절의 윤활막을 대상으로 정상과 퇴행성관절염 시의 윤활막의 변화와 tenascin의 발현을 면역전자현미경적 방법으로 비교, 관찰한 결과 다음과 같은 결과를 얻을 수 있었다. 1. 정상의 윤활막과 비교하여 퇴행성관절염 시에 윤활막이 두터워지는 것은 주로 윤활분비세포의 수적인 증가에 의한 것임을 알 수 있었다. 2. Tenascin에 대한 면역금의 관찰 결과 정상의 윤활막을 구성하는 세포에서는 면역금의 표지가 관찰되지 않았다. 3. 퇴행성관절염 시 윤활막의 윤활분비세포 과립세포질 세망에서 면역금의 표지가 관찰되어 tenascin의 분비세포가 윤활분비세포임을 확인할 수 있으며, 윤활세포 사이의 바탕질의 아교섬유에서도 tenascin의 표지가 관찰되었다. 이상의 결과를 종합해 보면, 퇴행성관절염 시에 윤활막이 두터워지는 것과 병변의 진행과정에, 윤활분비세포의 수가 증가와 tenascin의 발현 증가가 관련성이 있는 것으로 생각된다. To observe the morphological changes and the expression of tenascin in synovium of human knee joint, between normal condition and degenerative arthritis, were processed by immunoelectron microscopic method. The results were summarized as follows. 1. In degenerative arthritis, the hyperplasia of synovial membrane was characterized by the increase of cell number in secretory synovial cells. 2. In normal condition, there was no marking of the immuno-gold for tenascin in synovial membrane. 3. In degenerative arthritis, the immuno-gold for tenascin were observed in endoplasmic reticulum of secretory synovial cells and extracellular matrix of synovial layer. On the basis of above findings, the hyperplasia of synovial membrane and the pathologic processes may be concerned with the increase of number of secretory synovial cells and of expression of tenascin, in degenerative arthritis.

Lipopolysaccharide 로 유발된 생쥐 무릎관절낭 염증에 관한 형태학적 연구 : 윤활관절막과 섬유관절막의 변화를 중심으로 Based on the Morphological Changes of Synovial Membrane and Fibrous Membrane

김진택,안상현,최난희,정재만,박인식,강윤호,김호현,이해풍 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

본 연구는 관절염 유발시 일어나는 관절낭의 형태학적 변화를 조사하기위해 ljpopolysaccharide(LPS)주사로 인위적 관절낭 염증을 유발시킨 후 시간경과에 따른 윤활관절막과 섬유관절막의 형태 변화를 관찰하였다. BALB/C 암컷 생쥐 오른쪽 무릎관절낭에 LPS 300㎍/㎏를 주사한 후 3, 7 그리고 14일에 무릎관절을 얻었다. 무릎관절은 4주동안 EDTA용액에 탈회한 후 통상적 방법으로 paraffin에 포매하였다. 또한 윤활관절막의 미세구조변화는 embed812로 포매한 후 관찰하였다. LPS 주사후 관절연골 인접부위의 윤활관절막에서 시작된 세포과형성(hyperplasia)은 시간 경과후 전체 윤활관절막으로 확대되었다. 윤활관절막내의 미세구조의 변화로는 윤활포식세포(type 1)가 관절강내로의 많은 돌기(filopodia)를 내었고, 잘 발달된 과립형질내세망을 가지는 type 2 윤활분비세포의 숫적 증가가 보였다. 한편 LPS 주사후 섬유관절막에서 나타나는 형태학적 변화는 collagen fiber 생성에 의한 섬유화가 증가되며, 이러한 섬유화를 주도하는 섬유모세포의 이주증가파 관찰되었다. 또한 혈관 주위에서는 백혈구의 이주 증가가 나타났으며, 탈과립형(degranulated type) 비만세포가 많이 관찰되었다. 이상의 결과로 LPS 주사로 관절낭에서 염증이 유발되어 윤활관전막과 섬유관절막에서 형태학적 변화가 나타났다. 이러한 일련의 형태학적 변화는 발병초기 류마티스성 관절염에서 나타나는 병리학적 소건과 동일한 결과로서, 앞으로 진행될 치료제 개발과 유발기전에 관한 해석을 위한 in vivo 실험의 적절한 모델로 기여한 것으로 기대된다. Synovial joint of BALB/C mice were injeced with Lipopolysaccharide(LPS) were observed to investigate the morphological changes of synovial capsule caused by rheumatoid arthritis(RA). The RA on female Balb/c mice were induced by LPS injection, as dose of 300㎍/㎏, into synovial cavity of knee joint. And then these specimen were fixed in 10% neutral buffered formalin and were decalcificated in EDTA solution for 4 weeks. The hyperplasia of synovium were appeared in synovial membrane. The filopodia of phagocytic like synoviocyte(type Ⅰ synoviocyte) projected into synovial cavity and the number of fibroblast like synoviocyte(type Ⅱ synoviocyte) with well-developed endoplasmic reticulum were increased in synovium. In fibrous membrane, the fibrosis induced by synthesis of collagen fiber were enlarged to all fibrous membrane, and the number of fibroblast were increased. A great number of inflammation component cell as Iymphocyte and neutrophil leukocyte were infiltrated around capillary and the degranulate typed mast cell were increased. As results indicated that the hyperplasia of synovium induced by LPS, subsequently to cause the fibrosis, infiltration of imflammation component cell, and increase of degranulated type mast call as same as symptoms of RA.

홍화자유약침이 CIA 모델 생쥐의 윤활관절막 손상 억제에 미치는 영향

백성욱,김은정,황지후,윤종화,이승덕,김갑성 대한침구의학회 2012 대한침구의학회지 Vol.29 No.1

Objectives : The purpose of this study is to observe the inhibitory effects of Chrthami semen oil pharmacopuncture(CSOP) on CIA (collagen-induced arthritis) mice. Materials and Methods : Two types of experiments were conducted: in vitro assay, inhibition of MIF mRNA and TNF-α mRNA expressions in synovial membranes was observed, and in vivo assay, 1 ㎕/㎏ CSOP was injected every day to the left Weizhong (BL40) from day 3 to 21 after induction of CIA, and changes in paw edema, apical surface morphology, neovascularization in synovial membranes, fibrosis, pro-inflammatory cytokines production, Th-1 differentiation, and anti-inflammatory effect were investigated. Results : 1. In synoviocytes of the CIA mice treated with CSOP, MIF mRNA and TNF-α mRNA expressions were down-regulated in a dose-dependent manner. 2. Paw edema of the CIA mice treated with CSOP was diminished. 3. Tissue injury in the synovial membranes, capillary distribution and fibrosis were reduced in CSOP-treated mice. 4. MIF, TNF-α, IL-6, MMP-9 expressions were repressed in CSOP-treated mice during the experiment to observe the inhibitory effect on cytokines production in early stage RA. 5. IL-12 and CD28 were reduced in CSOP-treated mice during the observation of inhibitory effect on Th1 differentiation. 6. PPAR-γ was increased during the experiment to observe the anti-inflammatory effect of CSOP. Conclusions : The results may suggest that administration treatment using Chrthami semen oil pharmacopuncture decreases the inflammatory response on an Animal Model with CIA.

활액막 추벽 증후군과 정상 추벽에서의 활액막 추벽의 자기공명영상 소견

김한복 대한영상의학회 1996 대한영상의학회지 Vol.35 No.6

Purpose : To compare MRI appearance between plicae syndrome and normal plicae. Materials & Methods : MR imagesof 60 cases of arthroscopically-confirmed plicae syndrome and 18 of arthroscopically-proven normal plicae wereretrospectively analyzed. Sagittal T2-weighted MR images in all cases and MPGR(200) in 37 cases of plicae syndromewere obtained. Statistical analysis was performed using the chi-square test. Results : On the basis of operatingresults, we observed 55 medial plicae, eight combined medial and suprapatellar plicae, four suprapatellar plicae,and one lateral plica. T2-weighted sagittal MR scans of the 60 cases demonstrated 37 medial plicae, 8suprapatellar and one lateral plica. Joint effusion was found in 26 cases of 55 medial plicae. In T2-weightedsagittal MR scans, the identification of medial plicae was superior in the presence of joint effusion than itsabsence(plicae syndrome, p < 0.001 ; normal plicae group, p < 0.05). Medial plicae were well demonstrated onMPGR(200) axial images; on T2-weighted sagittal MR scans, they could be more frequently identified in the plicaesyndrome group than in the normal control group(p < 0.001). Plicae syndrome-associated pathology includeddegenerative change of the articular cartilage of the medial femoral condyle in eight cases(14.5%), discoidmeniscus in nine(16.4%), lateral meniscus tear in 12(21.8%), medial meniscus tear in 21(38.1%), anterior cruciateligament tear in three(5.5%), medial collateral ligament tear and osteochondritis dissecans in one case.Conclusion : The present study revealed that synovial plicae were well demonstrated in T2-weighted sagittalimages, particularly on the presence of joint effusion. Medial plicae could be more frequently identified in theplicae syndrome group than in the normal control group, especially on T2-weighted sagittal MR scans.

류마티스 관절염과 퇴행성 관절염의 활액막조직에서 VLA 항원의 발현에 관한 연구

유대현(Dae Hyun Yoo),정성수(Sung Soo Jung),이인홍(In Hong Lee),배상철(Sang Cheol Bae),김성윤(Seong Yoon Kim),김목현(Mok Hyun Kim),정현기(Hyun Kee Chung),최일용(Il Yong Choi) 대한내과학회 1994 대한내과학회지 Vol.46 No.5

N/A Objective: The adhesion molecule that mediate cell-cell and cell-extracellular matrix provides very important role in various physiologic and pathologic conditions, VLA integrin is heterodimeric transmembrane glycoprotein which participate in cell-cell and cell-extracellular matrix, especially collagen, laminin and fibronectin. Rheumatoid arthritis is important prototype of chronic inflammatory disease. Continuous emigration and anchorage of chronic inflammatory cells are prerequisite for the perpetuation of disease. Transient episode of inflammatory synovitis could be found in osteoarthritis also. Although the notion that VLA-integrin play a major role in the initiation and perpetuation of arthritis would be possible, distribution of VLA integrin in synovial tissue of rheumatoid arthritis and osteoarthritis was investigated by immunohistochemistry. Methods: Immunohistochemical analysis of synovial tissue utilizing avidin-biotin complex method was con- ducted. Applied monoclonal antibodies were CD49a-f, CD29, CD51, and CD61. The pattern of expression was scored by semiquantitative grading system. Results: 1) The expression of CD29 was diffuse and intense at synovial lining cells, endothelium and infiltrating inflammatory cells. Endothelial stain was evident with VLA-1, 6, CD51 and negative with VLA-4. Inflammatory cells expressed VLA-4, 5 intesively and synoviocytes expressed VLA-3, 5, 6 and CD51 as ligand for fibronectin and laminin. 2) CD29 expression of inflammatory cells were prominent around small blood vessles which showed strong stain of CD29 and located just beneath the synovial lining cells. 3) VLA-integrin expression in synvoium of osteoarthritis was smimilar to that of rheumatoid arthritis except little inflammatory cell infiltratilon and little proliferation of microvasculature. Conclusion: It was able to presume from above findings that VLA-integrin has been doing some role in the development and perpetuation of synovitis. Future perspectives in field of VLA-integrin may be exact process of intracellular signalling, functional aspect of integrin and regulation of expression by some cytokines and growth factors.

활액 육종의 자기공명영상 소견

이진주 대한영상의학회 1993 대한영상의학회지 Vol.29 No.5

MR images of seven patients with histologically documented synovial sarcoma were reviewed to find the suggestive or pathognomonic findings of the disease. MR appearance of the tumor was inhomogeneous. multiseptated mass with various degree of internal septation and infiltrative tumor margin. The signal intensity of the mass was slightly hyperintense relative to muscle on T1-weghted image and hyperintense relative to subcutaneous fatty tissue on T2-weighted image.

MRI Findings of Synovial Lipoma Arborescens of the Hip: A Case Report

박희진,박성일,박찬섭,김미성,박노혁,박재형,이상엽 대한영상의학회 2010 대한영상의학회지 Vol.63 No.6

Lipoma arborescens is a rare intra-articular lesion consisting of villous lipomatous proliferation of the synovium that tends to occur in the knee joint, especially in the suprapatellar pouch. The lesion has been observed in other locations, including the glenohumeral joint, subdeltoid bursa, hip, and elbow. We report a case of a lipoma arborescens of a unilateral hip joint in a 34-year-old man. MRI and a whole body bone scan were performed. In addition, a surgical synovectomy was performed and microscopic examination of the tumor revealed multiple variable-sized villous masses composed of mature fat tissue and lined by synovial cells which confirmed the diagnosis of lipoma arborescens.

A case of the metaplastic synovial cyst of the dorsum of the hand

( Chang Jin Jung ),( Chong Hyun Won ),( Sung Eun Chang ),( Mi Woo Lee ),( Woo Jin Lee ) 대한피부과학회 2021 대한피부과학회 학술발표대회집 Vol.72 No.2

Slug suppression induces apoptosis via Puma transactivation in rheumatoid arthritis fibroblast-like synoviocytes treated with hydrogen peroxide

Cha, Hoon-Suk,Bae, Eun-Kyung,Ahn, Joong-Kyong,Lee, Jae-Joon,Ahn, Kwang-Sung,Koh, Eun-Mi Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.6

Inadequate apoptosis contributes to synovial hyperplasia in rheumatoid arthritis (RA). Recent study shows that low expression of Puma might be partially responsible for the decreased apoptosis of fibroblast-like synoviocytes (FLS). Slug, a highly conserved zinc finger transcriptional repressor, is known to antagonize apoptosis of hematopoietic progenitor cells by repressing Puma transactivation. In this study, we examined the expression and function of Slug in RA FLS. Slug mRNA expression was measured in the synovial tissue (ST) and FLS obtained from RA and osteoarthritis patients. Slug and Puma mRNA expression in FLS by apoptotic stimuli were measured by real-time PCR analysis. FLS were transfected with control siRNA or Slug siRNA. Apoptosis was quantified by trypan blue exclusion, DNA fragmentation and caspase-3 assay. RA ST expressed higher level of Slug mRNA compared with osteoarthritis ST. Slug was significantly induced by hydrogen peroxide ($H_2O_2$) but not by exogenous p53 in RA FLS. Puma induction by $H_2O_2$ stimulation was significantly higher in Slug siRNA-transfected FLS compared with control siRNA-transfected FLS. After $H_2O_2$ stimulation, viable cell number was significantly lower in Slug siRNA-transfected FLS compared with control siRNA-transfected FLS. Apoptosis enhancing effect of Slug siRNA was further confirmed by ELISA that detects cytoplasmic histone-associated DNA fragments and caspase-3 assay. These data demonstrate that Slug is overexpressed in RA ST and that suppression of Slug gene facilitates apoptosis of FLS by increasing Puma transactivation. Slug may therefore represent a potential therapeutic target in RA.

Slug suppression induces apoptosis via Puma transactivation in rheumatoid arthritis fibroblast-like synoviocytes treated with hydrogen peroxide

Hoon-Suk Cha,안중경,Jaejoon Lee,안광성,Eun-Mi Koh,Eun-Kyung Bae 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.6

Inadequate apoptosis contributes to synovial hyperplasia in rheumatoid arthritis (RA). Recent study shows that low expression of Puma might be partially responsible for the decreased apoptosis of fibroblast-like synoviocytes (FLS). Slug, a highly conserved zinc finger transcriptional repressor, is known to antagonize apoptosis of hematopoietic progenitor cells by repressing Puma transactivation. In this study,we examined the expression and function of Slug in RA FLS. Slug mRNA expression was measured in the synovial tissue (ST) and FLS obtained from RA and osteoarthritis patients. Slug and Puma mRNA expression in FLS by apoptotic stimuli were measured by real-time PCR analysis. FLS were transfected with control siRNA or Slug siRNA. Apoptosis was quantified by trypan blue exclusion, DNA fragmentation and caspase-3assay. RA ST expressed higher level of Slug mRNA compared with osteoarthritis ST. Slug was significantly induced by hydrogen peroxide (H2O2) but not by exogenous p53 in RA FLS. Puma induction by H2O2stimulation was significantly higher in Slug siRNA-transfected FLS compared with control siRNA-transfected FLS. After H2O2 stimulation, viable cell number was significantly lower in Slug siRNA-transfected FLS compared with control siRNA-transfected FLS. Apoptosis enhancing effect of Slug siRNA was further confirmed by ELISA that detects cytoplasmic histone-associated DNA fragments and caspase-3 assay. These data demonstrate that Slug is overexpressed in RA ST and that suppression of Slug gene facilitates apoptosis of FLS by increasing Puma transactivation. Slug may therefore represent a potential therapeutic target in RA.