한국 야생 등줄쥐의 고해상도 염색체분염상

오승현,윤여성,진희경,성제경,Oh, Seung-hyun,Yoon, Yeo-sung,Jin, Hee-Kyung,Seong, Je-kyung 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.2

We investigated the cytogenetic characteristics of male black-striped field mouse (Apodemus agrarium) in Korea. Chromosome slides were obtained from blood cell cultures which were synchronized with thymidine blocking or not. In the chromosome slide which synchronization with thymidine blocking was employed on, the GTG(G bands by trypsin using Giemsa)-bands of high resolution were observed. The male black-striped field mouse has 48 chromosomes composed 46 autosomes and XY sex chromosomes. The centromeric regions of autosomes were positive to GTG-banding. According to this investigation, thymidine blocking in cell culture process was useful to get lengthened chromosomes. It may be necessary to employ RBG-banding technique to investigate complementary band patterns between R- and G-banding in black-striped field mouse.

Biogeography, Genetic Structure, and Speciation in the Striped Field Mouse, Apodemus agrarius, in Southern Korea

Yoon, Myung-Hee,Phillips, Carleton. J.,Kim, Iksoo,Oh, Hong Sik 한국유전학회 2004 Genes & Genomics Vol.26 No.1

The biogeography and population genetic structure in the striped field mouse, Apodemus agrarius, were investigated on the southern Korean mainland, coastal islets of Wan, Keoje, and Kaduk, and the offshore island, Cheju. DNA sequences from the mitochondrial cytochrome b protein-coding gene were used to test hypotheses based on previous investigations of these mice. We found 28 mtDNA-sequence-based haplotypes with maximum divergence of 2.0%. A PAUP analysis revealed at least six lineages with a southwest (Cheju Island) to northeast (the Korean mainland) polarity, The hypothesized oldest haplotypes were obtained on Cheju Island. Considering of the data in context of Late Pleistocent-Holocene landforms suggests that present-day Cheju Island is the remant of a refugium for A. agrarius. The Wan and Keoje islet haplotypes were monophyletic, whereas those on Cheju Island were not. In the broad view, significant genetic structuring was found among Cheju Island, islets, and mainland, but on the mainland itself there was no regional structuring. Genetic distance estimates suggest weak dispersal capability across narrow (< 2 km) coastal water gaps, but reprocuctive isolating mechanisms offer an alternative explanation. Overall, the data set is consistent with an earlier hypothesis that speciation has occurred, but our interpretation is that mainland A. agrarius coreae was derived from a coastal islet and offshore species, A. "chejuensis." This conclusion is a rare instance in which present-day island populations were the source of a mainland species.

보문 : 털진드기 흡혈에 의한 등줄쥐 피부의 흡입관 형성 관찰

이인용 ( In Yong Lee ),윤상선 ( Sang Sun Yoon ),이원자 ( Won Ja Lee ),신희관 ( Hee Kwan Sin ),이원구 ( Won Koo Lee ) 한국토양동물학회 2006 한국토양동물학회지 Vol.11 No.1,2

Echinostome Infections in the Striped-Field Mouse, Apodemus agrarius, and the Ussuri White-Toothed Shrew, Crocidura lasiura, Caught Near the Demilitarized Zone, Gyeonggi-do (Province), Republic of Korea

Jong-Yil Chai,Jae-Hwan Park,Bong-Kwang Jung,Sang-Mee Guk,Jae-Lip Kim,Eun-Hee Shin,Terry A. Klein,Heung-Chul Kim,Sung-Tae Chong,Luck Ju Baek,Jin-Won Song 대한기생충학열대의학회 2009 The Korean Journal of Parasitology Vol.47 No.3

A total of 1,498 small mammals (rodents and insectivores), including Apodemus agrarius (n = 1,366), Crocidura lasiura (54), Mus musculus (32), Micronytus fortis (28), Eothenomys regulus (9), Micronys minutes (6), and Cricetulus triton (3), were live-trapped in Gyeonggi-do (Province) (Paju-si, Pocheon-gun, and Yeoncheon-gun) near the demilitarized zone (DMZ) from December 2004 to September 2005. A. agrarius was found to be infected with 3 species of echinostomes (Echinostoma hortense, Echinostoma cinetorchis, and Euparyphium murinum), while C. lasiura was infected with 1 species (Echinochasmus japonicas) of echinostome. Other mammals were free from echinostome infections. Total 16 E. hortense were detected in 7 (0.5%) mice, 9 E. cinetorchis from 5 (0.4%), and 3 E. murinum from 2 (0.1%) out of 1.366 A. agrarius examined. E. japonicus was found only in 1 (1.9%; total 3 specimens) C. lasiura. These results demonstrate that A. agrarius and C. lasiura, inhabiting near the DMZ of Gyeonggi-do serve as the natural definitive hosts for several species of echinostomes, although their infection rates are low. This is the first record of natural infections of A. agrarius with E. cinetorchis and C. lasiura with E. japonicus in the Republic of Korea.

Genetic differentiation of the Korean striped field mouse, <i>Apodemus agrarius</i> (Muridae, Rodentia), based on microsatellite polymorphism

Jo, Yeong-Seok,Kim, Han-Na,Baccus, John T.,Jung, Jongwoo De Gruyter 2017 Mammalia Vol.81 No.3

<P><B>Abstract</B></P><P>The striped field mouse (<I>Apodemus agrarius</I>) is one of the most common mammals in Korea, however, limited research has focused on the population structure and biogeography of this mouse throughout Korea. In addition, the taxonomic status of the species remains unresolved. The objectives of our study were to address the inadequacies in understanding the population structure, biogeography, and taxonomic status of<I>A. agrarius</I>. We collected 160 samples from 13 localities throughout the South Korean Peninsula, two nearby islands and two remote islands. We used five microsatellite loci and estimated genetic diversity measures and populations divergence estimates (i.e.<I>F</I><SUB>ST</SUB>and<I>R</I><SUB>ST</SUB>) to address the objectives. Except for populations on the two remote islands, Jeju and Heuksan, the populations on the peninsula and nearby islands indicated low<I>F</I><SUB>ST</SUB>and<I>R</I><SUB>ST</SUB>values. The population structure based on microsatellite loci supported only one subspecies opposing three subspecies reported previously on the Korean Peninsula and nearby islands. However, populations on Jeju Island and Heuksan Island showed subspecific differences from each other and the populations on the Korean Peninsula and nearby islands. The information reported on the population genetics of the striped field mouse in Korea will allow resolving the taxonomy and biogeography of this species.</P>

4-t-octylphenol과 nonylphenol이 등줄쥐의 번식에 미치는 영향

김지혜 ( Ji Hye Kim ),윤명희 ( Myung Hee Yoon ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.2

To investigate the effects of octylphenol and nonylphenol on the reproduction of the striped field mouse, the mice were subcutaneousely injected with 4-t-octylphenol 800 mg/kg (OP800), nonylphenol 900 mg/kg (NP900) or OP800 + NP900 (OPNP), respectively, in two times a week for 2 months. As the results, there were no differences between the control and the compound-treated groups in the body weight in both sexes, and the GSI (gonadosomatic index) and SVI (seminal vesicles index) in the adult males. But histological abnormalities of the reproductive organs in the mice seems to be related to the compound. This suggestion is supported by the facts that most mice treated with the compounds had only a small number of spermatozoa in the shrunken epididymal tubules. In addition, the shrunken reproductive organ in one mouse treated with the NP, suggests that the abnormalities in the wild striped field mice might be induced by the compound. Furthermore, it is indicated that the compounds must be toxicants to inhibit pregnancy, judging from the fact that all the mice treated with the compounds had failed to deliver.

Biological characterization of korean striped field mouse

Hye Jin Kim,Su Hyun Lim,Kyungmin Choi,Mi-gyeong Kim,Hee Jung Shin,Young Min Cho,Hae Deun Kim,Won Hee Lee,Hye-Jun Shin,Myeon-Woo Chung 한국실험동물학회 2017 한국실험동물학회 학술발표대회 논문집 Vol.2017 No.2

한국산 애생등줄쥐 (Apodemus Agrarius coreae)의 대뇌피질과 줄무늬체에서 Neuropeptide Y 면역반응신경세포의 분포

이경열,박일권,송치원,권효정,박미선,김무강,정영길,이강이,이철호,현병화,최농훈 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.2

한국산 야생등줄쥐 (Apodemus Agrarius coreae)의 대뇌피질과 줄무늬체에서 Neuropeptide Y 면역반응신경세포의 분포

이경열(Kyoung-Youl Lee),박일권(Il-Kwon Park),송치원(Chi-Won Song),권효정(Hyo-Jung Kwon),박미선(Mi-Sun Park),김무강, 정영길(Young-Gil Jung),이강이(Kang-Iee Lee),이철호(Chun-Ho Lee),현병화(Byung-Hwa Hyun),최농훈(Nong-Hun Choe) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.2

Neuropeptide Y (NPY)는 돼지의 뇌에서 처음 분리 추출된 이후 여러학자들에 의해 여러동물의 뇌에 존재함이 밝혀졌으 며 이런 NPY는 신경조절물질 또는 신경활성물질에 관여하는 것으로 있려져 있다. 본 연구에서는 한국산 야생등줄쥐 (apodemus agrarius cereae)의 대뇌피질과 줄무늬체에서의 NPY면역반응신경세포체의 분포를 관찰하고자 면역조직화학염 색을 실시하여 관찰하였던 바 다음과 같은 결론을 얻었다. 1. 야생등줄쥐의 대뇌피질에서 NPY면역반응신경세포체가 모든 층에서 출현하였으며 특히 V층과 VI층에 주로 분포하 였고 I층과 VI층에서는 드물게 나타났다. 2. 대뇌피질에서 NPY면역반응신경세포체의 모양은 타원형, 난원형, 뭇각형 등 다양하게 존재하였고 I층과 II층에서는 원 형과 타원형이 많았으며 V층과 VI층에서는 뭇각형이 많았다. 세포돌기는 두극형 또는 뭇극형으로 존재하였는데 그 세포돌기의 주행방향이 I층과 II층에서는 연질막 (pia mater)과 평행으로 III층에서 두극형의 세포는 수직방향으로 그 돌기가 뻗어 있었으며 VI층에서는 뇌량과 평행하게 주행하고 있었다. 세포체의 크기는 중간크기 (10~20 μm)의 세포가 대부분이고 20 μm 이상인 세포는 드물게 관찰되었다. 3. 대뇌피질의 부위별 분포는 후각뇌주위피질에서 그 수가 가장 많이 나타났고 두정피질에서 그 다음으로 많이 나타났 으며 팽대뒤피질에서는 가장 적은 것으로 나타났다. 그러나 섬피질에서는 갯수는 적으나 그 밀집도가 높았다. 두정피 질부위에서는 대개 III-VI층에 주로 분포하였고 전두피질에서는 다른 부위보다 II층에 많은 세포체가 존재하였다. 4. 줄무늬체에서의 NPY면역반응신경세포체는 꼬리-조가비핵에서만 관찰되었으며 뒷쪽으로 갈수록 그 수가 줄어들었고 중간부위에서는 NPY면역반응신경세포체가 배쪽내측부분에 많이 분포되어 있는 것을 볼 수 있었다. 5. 줄무늬체에서의 NPY면역반응신경세포체의 모양은 타원형, 난원형, 뭇각형 등 다양하였으나 주로 뭇각형이 많았으며 그 세포돌기도 사방으로 뻗어있었고 그 크기는 15~20 μm로 중간세포가 대부분이었다. Neuropeptide Y (NPY) was first isolated from porcine brain. This discovery has led some workers to study the distribution of this peptide in the central nervous system of various mammals. In this study examined the distribution pattern of NPY-immunoreactive (NPY-IR) neurons in the Striped Field Mouse (Apodemus agrarius coreae) cerebral cortex and striatum, using immunohistochemical method. The results obtained in this study were summarized as followings. 1. NPY-IR neurons distributed in all layer of cerebral cortex. The number of neurons were higher in layer V and VI than in layer I and IV. 2. The shape of neurons was predominantly round or oval in layer I and II, and triangular in layer V and VI. And the processes were parallel to pia mater in layer I and II and were vertical in layer III. 3. The highest number of NPY-IR neurons were found in the perirhinal cortex but a few distinct population were found in the retrosplenial cortex. 4. In stiatum NPY-IR neurons were observed only in caudate-putamen nucleus. 5. The Immunoreactive neurons in caudate-putamen varied in their shape, but most of them were triangular or multiform neurons had omnidirectional processes.

등줄쥐(Apodemus agrarius Pallsa)에 있어서 α-amylase의 특성연구(Ⅱ) : 당뇨 모델동물로서 등줄쥐의 유용성

채갑용,임철주,김용규,김철규,장인석,황진희,황대연,조성화,이은,조정식 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-

등줄쥐를 실험동물로서 유풍성을 증진하고 당뇨 모델동물간의 생리적 발칠 특성을 연구하기 위하여 비만 감수성 동물에 특이적으로 비만을 유발하는 사료 모델인 고지방사료(34% 지방에너지/총에너지)를 등줄쥐 및 당뇨 모델동물(KK 마우스와 chinese hamster)에 급여하여 당뇨 및 비만의생리적 특성을 연구하였다. 각 동물은 4-6주령의 수컷 24마리, 총 72마리를 10준간 급여하였다. 이들의 결과 빅만 감꼭성 모질사료인 고찌방사료 급여에 의하예 딘K마 우스는 고혈당,혈중 지질함량 및 leptin이 현저한 증가가 나타나인간의 비만형 당뇨모델동물로서 고지방에 민감한 반응을 나타내고 있는 반면에 IDDM이 다발하는 chinese hamster에서는 영향을 나타나지 않는 것으로 나타났고 등줄쥐는 KK마우스 및 chinese hamster에 비하여 혈중 중성지질 및 cholesterol 함량이 현저히 낮고 고지방 사료에는 민감한 반응을 나타내지 깥아 지방대사에는 현저한 항상성은 있으나 혈당 및 혈중 인슬린 함량이 높은 당뇨모질동물로서 가능성을 시사하고 있다. The levels of enzyme activity (o-amylase), metabolite (serum glucose, cholesterol, triglyceride) and peptide hormones (insulin, leptin) were exarBined fn striped field mice and the diabetesmodel animals fed moderatelr high fat diet for 10 weeks. Four-six weeks old of male animal in stripedfield mice, KK mice and chinese hamsters were used in this experimrnt and fed pelleted diet containiBgmoderately high fat diet wifh 34% of energy supplied as fat. At the ending 70 days of feeding, blood wasquickly collected from hea3f puncture and parotid gland, pancreas, liver and kidneys were removed.Serum were prepared for engfme activity, metabolite and peptide hot·none levels. As a result of experi-meBt, a moderately high fElt diet gave rise to significant increase in blood glucose, lipid and leptin i8KK mice, kRown as a mode:1 animal for human diabetes(non- insulin dependent diabetes Taellitvs). How-ever, a high fat diet did not induce a sigBifincantly change in chinese hamsters knoweB as a insulin de-Thus, strired field mice indicated a potentially useful aBiinal model for diabetes to studf of mechanismunderlying in development of diabetes.