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      • KCI등재후보

        Investigation of optimal in vitro and ex vitro conditions for the development of Athyrium acutipinnulum Kodama synthetic seed technology

        이하민,장보국,박경태,조주성,이철희 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.1

        Athyrium acutipinnulum Kodama ex Nakai is an edible fern that is native to Ulleung island and Gangwon Province (Taebaek Mountain), South Korea. A. acutipinnulum has high nutritional value and is rich in protein, carbohydrates, vita mins, minerals, and nicotinic acid. However, the cultivation of A. acutipinnulum is generally carried out on a small scale. In addition, the current rhizome propagation method is labor intensive and ineffi cient with regard to root growth-point establishment. The present study was conducted to improve the effi ciency of the A. acutipinnulum cultivation method via in vitro culture and synthetic seed technology. To select an eff ective medium for in vitro proliferation, the concentrations of mineral salts, sucrose, activated charcoal, and total nitrogen in Murashige and Skoog (MS) media were adjusted. The 1 MS medium (3% sucrose, 60 mM total nitrogen, 0.8% agar, pH 5.8), had the highest gametophyte proliferation. Spo rophyte formation and growth were outstanding in the mixed substrate including horticultural substrate and decomposed granite 2:1. The production of SFG (synthetic seeds using fern gametophyte) using the proliferated gametophyte led to a percent of sporophyte formation of 76%. Plant growth regulators were added in SFG in order to increase the percent of sporophyte formation. Gibberellin acid (GA 3 ) added SFG showed an increase in the percent sporophyte formation to more than 90%. The present study explored the optimal growth medium conditions for A. acutipinnulum gametophytes to use for SFG. SFG were successfully produced and their conditions for the massive sporophyte formation were confi rmed.

      • KCI등재

        Sprouting Condition of Crown Bud and Plug Seedling Production in Yacon

        Doo, Hong-Soo,Choo, Byung-Kil,Ryu, Jeom-Ho The Korean Society of Crop Science 1998 Korean journal of crop science Vol.43 No.4

        The objectives of this study were to find the sprouting condition and to establish the optimum production methods of plug seedlings in yacon (Polymnia sonchifolia Poepp. & Endl.). The sprouting ratio was greatest at $30\pm 1^{\circ}C$ at 20 days after planting. Crowns with single buds were more effective than those with two or more buds for sprouting, which might be due to the apical dominance. Planting the shoots separated from crown after sprouting in the single- and double-layer polyethylene-covered greenhouses reduced seedling period with 25% and 50%, respectively. Planting the shoots after sprouting was more effective than planting the crown buds. Double-layer polyethylene-covered green-house was good for plug seedling production than open field or single-layer polyethylene-covered greenhouse. The bed soils composed of clay loam : compost or sand : compost (1:l=v:v) were more effective to produce plug seedlings than only clay loam, sand or compost. Seedlings could be produced at 30 days after planting in our studies.

      • KCI등재

        Sprouting Condition of Crown Bud and Plug Seedling Production in Yacon

        Hong Soo Doo,Byung Kil Choo,Jeom Ho Ryu 韓國作物學會 1998 Korean journal of crop science Vol.43 No.4

        The objectives of this study were to find the sprouting condition and to establish the optimum production methods of plug seedlings in yacon (Polymnia sonchifolia Poepp. & Endl.). The sprouting ratio was greatest at 30~pm 1~circC at 20 days after planting. Crowns with single buds were more effective than those with two or more buds for sprouting, which might be due to the apical dominance. Planting the shoots separated from crown after sprouting in the single- and double-layer polyethylene-covered greenhouses reduced seedling period with 25% and 50%, respectively. Planting the shoots after sprouting was more effective than planting the crown buds. Double-layer polyethylene-covered green-house was good for plug seedling production than open field or single-layer polyethylene-covered greenhouse. The bed soils composed of clay loam : compost or sand : compost (1:l=v:v) were more effective to produce plug seedlings than only clay loam, sand or compost. Seedlings could be produced at 30 days after planting in our studies

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