만성전립선염 환자에서 정장액 Cholesterol과 Phospholipid 농도의 변화

송윤섭,허만우,이국현,이수형,김민의,박영호,이무상,최형기 大韓泌尿器科學會 1998 Korean Journal of Urology Vol.39 No.1

Controlled release of astaxanthin from nanoporous silicified-phospholipids assembled boron nitride complex for cosmetic applications

Lee, Hye Sun,Sung, Dae Kyung,Kim, Sung Hyun,Choi, Won Il,Hwang, Ee Tag,Choi, Doo Jin,Chang, Jeong Ho Elsevier 2017 APPLIED SURFACE SCIENCE - Vol.424 No.1

<P><B>Abstract</B></P> <P>Nanoporous silicified-phospholipids assembled boron nitride (nSPLs@BN) powder was prepared and demonstrated for use in controlled release of anti-oxidant astaxanthin (AX) as a cosmetic application. The nanoporous silicified phospholipids (nSPLs) were obtained by the silicification with tetraethyl orthosilicate (TEOS) of the hydrophilic region of phospholipid bilayers. This process involved the co-assembly of chemically active phospholipid bilayers within the porous silica matrix. In addition, nSPLs@BN was characterized using several analytical techniques and tested to assess their efficiency as drug delivery systems. We calculated the maximum release amounts as a function of time and various pH. The release rate of AX from the nSPLs@BN for the initial 24h was 10.7μmol/(hmg) at pH 7.4. Furthermore, we determined the antioxidant activity (<I>K</I> <SUB> <I>D</I> </SUB>) for the released AX with DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical and the result was 34.6%.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Nanoporous silicified phospholipids particles are enhancement of rigidity by the silicification. </LI> <LI> Nobel nSPLs@BN are synthesized and demonstrated for the controlled astaxanthin release. </LI> <LI> AX-release and anti-oxidant activity were achieved as a function of time and various pH. </LI> <LI> Release amounts and anti-oxidant activity were 10.7μmol/(hmg) and 34.6%, respectively. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Schematic of the (a) boron nitride powders (BN), (b) magnesium chelated boron nitride powders (Mg@BN), (c) phospholipids-coated boron nitride powders (PLs@BN), and (d) nanoporous silicified phospholipids-coated boron nitride powders (nSPLs@BN).</P> <P>[DISPLAY OMISSION]</P>

Effects of Dietary Gum Phospholipid on Lipid Metabolism in Broiler Chicks

An, B.K.,Kang, C.W.,Nishiyama, H.,Iwata, T.,Tanaka, K. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.4

This experiment was to determine the usefulness of gum safflower phospholipid as a feed ingredient. Forty female broiler chicks were divided into four groups and fed experimental diets containing following fats and oils; beef tallow (Tallow), the blend of safflower oil and palm oil (SP-oil), gum rapeseed phospholipid (Rap-PL), or gum safflower phospholipid (Saf-PL) for 21days. There were no differences in growth performances among the treatments. Abdominal fat weight tended to be reduced in the chicks fed. phospholipids. The activity of hepatic acetyl-CoA carboxylase was significantly reduced in the Rap-PL and Saf-PL as compared to that of Tallow. Feeding dietary phospholipids resulted in a slight reduction in total fat and triglyceride contents in the breast and thigh muscles. In addition, total fat and triglyceride contents in the thigh muscle were significantly decreased by dietary Saf-PL as compared to those of Tallow. These results suggested that dietary gum phospholipids, either from rapeseed or safflower, had desirable effects of lowing abdominal and muscle fats, and could be used as a feed ingredient for broiler diets.

Alteration of Phospholipids during the Mitophagic Process in Lung Cancer Cells

( Jae Won Lee ),( Kyung Mi Cho ),( Jae Hun Jung ),( Quangdon Tran ),( Woong Jung ),( Jongsun Park ),( Kwang Pyo Kim ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.10

Matrix assisted laser desorption ionization (MALDI)-time of flight/mass spectrometry (TOF/MS) was applied to investigate alterations in phospholipids in mitophagic cancer cells. Several phospholipids, including phosphatidylcholines (PCs), sphingomyelins (SMs), and phosphatidylinositols (PIs), were successfully analyzed in control and mitophagy-induced H460 cells in the positive and negative ion modes. Principal component analysis was applied to differentiate the two groups. The upregulated and downregulated phospholipid species in the mitophagic cells were also represented in a heatmap. In the volcano plot (fold change > 1.3 and p value < 0.01), individual species of seven PCs, two SMs, and three PIs were selected as differentially regulated phospholipids. In particular, almost all the molecular species of PC, SM, and PI were downregulated in the mitophagic cells. Quantification of these lipids indicated that mitophagy induces altered metabolism of phospholipids. Therefore, phospholipid alterations during the mitophagic process of lung cancer cells were well characterized by MALDI-TOF/MS.

Orotic acid 투여가 흰쥐의 혈청, 肝臟 및 腎臟 지질 농도에 미치는 영향

김석환,車載英,조영수 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4

Orotic acid 의 과잉 섭취는 지질대사의 이상으로 인한 간 장해를 야기시키는 것이 알려져있다. 특히 지방간 생성에 대하여 관심을 가지게 되어서 본 연구는 orotic acid에 의한 혈청, 腎臟및 肝臟의4 지질 농도에 미치는 영향에 대하여 검토하였다. 시판 분말 chow 식이에 orpotic acid 1% 첨가, 무첨가한 식이를 21일간 90g전후의 성장기 Spragud-Dawley 系 웅성 쥐에 급여하였다. 그 결과 orotic acid 1% 첨가 식이에서 혈청 cholesterol, triglyceride및 phospholipid 농도는 각각 5% 수준에서 유의하게 저하하였다. 肝臟의 triglyceride농도는 5% 수준에서 유의하게 저하하였다, Orotic acid 첨가 식이에서 肝臟중량은 증가한 반면 腎臟중량은 저하하였다. Orotic acid 1% 첨가한 군에서 肝臟및 腎臟의 phospholipid 조성에의 영향은 인정되지 않았으나, 腎臟의 lysophosphatidylcholine은 높은 증가율을 보였다. 肝臟 phospholipid와 비교하여 腎臟에서는 sphingomyeline phospphatidylethanolamin은 유의하게 높은 분포를 나타냈다. 腎臟 phospholipid의 지방산 조성중에서 linoleic acid(18:2)가 상승하고 arachidonic acid (20:4)가 감소하였다. It was generally known that over-ingestion of dietary orotic acid caused hepatic disorder by the lesion of fatty acid and cholesterol synthesis in rats. The present study was carried out to investigate the effects of dietary orotic acid on the lipid composition of serum. liver and kidney of rats. For the experiments rats were fed with commercialized chow powder diet in the presence or absence of 1% orotic acid. The prepared diets were fed to male rats (Sprague-Dawley strain. 90∼100g of body weight) for 21 days. According to the results, orotic acid treated group showed that each concentration of serum cholesterol, triglyceride and phospholipid was significantly decreased (p<0.05). The centration of liver triglyceride was significantly decreased (p<0.05). In the presence of 1% orotic acid, the weight of liver increased while that kidney decreased. The treatment of orotic acid seemed to have no effects on the phospholipid composition in liver and kidney, except the kidney lysophosphatidylcholine. In the comparison of the phospholipid composition between liver and kidney, the levels of sphingomyeline and phosphtidylethanolamine in the kidney were higher than those in the liver. Among the composition of fatty acid in kidney, the concentration of linoleic acid (18:2) was increased, and the concentration of arachidonic acid(20:4) was decreased with the addition of the orotic acid.

이담자균 효모의 성분화 과정중 인지질의 작용과 배지조성의 제한이 성분화에 미치는 영향

정영기,강원대,남수완 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1

The action of phospholipid on the rhodotorucine A(Rh.A) acceptance by heterobasidiomyceteous yeast Rhodospori-dium toruloides mating type a cells and the effect of medium composition during sexual differentiation were investigated. Activation of trigger peptidase(TPase) was very sensitive to the originated phospholipid from R. toruloides and was more sensitive to phospholipid liposome made up of phospholipid. Phospholipid present on the membrane of mating type a cells consists of phospatidylglycerol(PG), phosphatidylethanolamine(PE), phospatidylcholine(PC), phospatidylinositol(PI), and phosphatidylserine(PS) of 12.9, suprisingly 45.4, 11.0, and 13.9%, respectively. As the result of using C-1 and N-1 mediums which limited C and N sources capable of inhibiting the synthesis of phospholipid. it resulted inhibiting sexual differentiation and production of Rh.A from mating type A cells.

Orotic acid 투어가 흰쥐의 혈청, 간장(肝臟) 및 신장(腎臟) 지질 농도에 미치는 영향

김석환,차재영,조영수,Kim, Seok-Hwan,Cha, Jae-Young,Cho, Young-Su 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.1

Orotic acid의 과잉 섭취는 지질대사의 이상으로 인한 간 장해를 야기시키는 것이 알려져있다. 특히 지방간 생성에 대하여 관심을 가지게 되어서 본 연구는 orotic acid에 의한 혈청, 신장(腎臟) 및 간장(肝臟)의 지질 농도에 미치는 영향에 대하여 검토하였다. 시판 분말 chow 식이에 orotic acid 1% 첨가, 무첨가한 식이를 21일간 90g 전후의 성장기 Sprague-Dawley 계(系) 웅성 쥐에 급여하였다. 그 결과 orotic acid 1% 첨가 식이에서 혈청cholesterol, triglyceride 및 phospholipid 농도는 각각 5% 수준에서 유의하게 저하 하였다. 간장(肝臟)의 triglyceride 농도는 5% 수준에서 유의하게 저하 하였다. Orotic acid 첨가 식이에서 간장(肝臟)중량은 증가한 반면 신장(腎臟)중량은 저하하였다. Orotic acid 1% 첨가한 군에서 간장(肝臟) 및 신장(腎臟)의 phospholipid조성에의 영향은 인정되지 않았으나, 신장(腎臟)의 lysophosphatidylcholine은 높은 증가율을 보였다. 간장(肝臟) phospholipid와 비교하여 신장(腎臟)에서는 sphingomyeline phosphatidylethanolamine은 유의하게 높은 분포를 나타냈다. 신장(腎臟) phospholipid의 지방산 조성중에서 linoleic acid(18:2)가 상승하고 arachidonic acid(20:4)가 감소하였다. It was generally known that over-ingestion of dietary orotic acid caused hepatic disorder by the lesion of fatty acid and cholesterol synthesis in rats. The present study was carried out to investigate the effects of dietary orotic acid on the lipid composition of serum, liver and kidney of rats. For the experiments, rats were fed with commercialized chow powder diet in the presence or absence of 1% orotic acid. The prepared diets were fed to male rats (Sprague-Dawley strain, $90{\sim}100$ g of body weight for 21 days. According to the results, orotic acid treated group showed that each concentration of serum cholesterol, triglyceride and phospholipid was significantly decreased (p<0.05). The centration of liver triglyceride was significantly decreased (p<0.05). In the presence of 1% orotic acid, the weight of liver inclosed while that of kidney decreased. The treatment of orotic acid seemed to have no effects on the phospholipid composition in liver and kidney, except the kidney lysophosphatidylcholine. In the comparison of the phospholipid composition between liver and kidney, the levels of sphingomyeline and phosphtidylethanolamine in the kidney were higher than those in the liver. Among the composition of fatty acid in kidney, the concentration of linoleic acid (18 : 2) was increased, and the concentration of arachidonic acid (20 : 4) was decreased with the addition of the orotic acid.

Combined Phospholipase and Lipase Catalysis for Biodiesel Production from Phospholipids-containing Oil

Yang Li,Yanfei Huang,Wei Du,Lingmei Dai,Dehua Liu 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.5

Free lipase-mediated biodiesel production has been considered to be promising due to its advantages of high catalytic efficiency and lower preparation cost. Exploring the feasibility of free lipase to convert potential low quality oil feedstock into biodiesel is of great significance for further reducing the cost of biodiesel production. However, it is reported that low quality oils usually contain high concentration of phospholipids. Our previous study showed that the presence of high phospholipids content in oil feedstock would lead to poor catalytic performance of free lipase NS81006.Thereby, in order to improve the process, a combined catalysis together with phospholipase Lecitase Ultra and lipase NS81006 was developed in this paper. First, the effect of different factors involved in the process on Lecitase Ultra’s catalytic performance was investigated, then a two-step method via phospholipase-catalyzed phospholipids degradation followed by lipase-catalyzed methanolysis was further attempted to promote the conversion of phospholipids-containing oils for biodiesel production. When using oil containing 2,235 ppm initial phosphorus as feedstock, the final biodiesel yield could reach 96.4%, while the yield without phospholipase was only 76.6%. This work demonstrates that the combined catalysis of phospholipase and free lipase has a great prospect in biodiesel production from high phospholipids-containing oil feedstocks.

효소 Lipoxygenase의 신규기질 : Acylglycerol, acylethanolamide, lysophospholipids 및 phospholipids

황룡쌍,류희문,박천호,석대은 충남대학교 약학대학 의약품개발연구소 2007 藥學論文集 Vol.22 No.-

Lipoxygenase belongs to a diverse family of nonheme ferroproteins that oxygenate polyenoic fatty acids containing 1,4-pentadiene structure to form their corresponding hydroperoxy derivatives. Lipoxygenases (LOXs), widely distributed in animals and plants, have a key function in the formation of biologically active substances from pulyunsaturated fatty acids. Generally, free polyunsaturated fatty acids, liberated from membrane phospholipids via phospholipase-catalyzed hydrolysis, are used as substrates for LOXs. Although it is acknowledged that free polyunsaturated fatty acids are preferred to phospholipids or triglycerides as substrates, there have been recent reports that mammalian enzymes can oxidize certain phospholipids. Especially, reticulocyte LOX (15-LOX) leukocyte 15-LOX, leukocyte LOX (12-LOX) can oxygenate complex substrates such as phospholipids and biomembranes. In addition, acylglycerol and acylethanolamide are utilized by lipoxygeanse as well as cycoloxygenase; the latter enzyme contributes to generation of bioactive prostanoids derivative. Furthermore, lysophosphatidylcholine or lysophosphatidic acid containing linoleoyl or arachidonoyl moieties are known to be oxygenated by reticulocyte LOX, leukocyte 15-LOX or leukocyte-type 12-LOX; oxygenated lysophospholipids can play a carrier role in transporting oxygenated derivatives. Thus, the use of various lipid substrates as new substrates for lipoxygenase may extend the physiological roles of those lipids containing unsaturated fatty acyl chains.

Visualizing phospholipid inhibition mechanism by highly stable albumin layer at the air-water interface

박수진,이현로,최시영 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0

We visualized the albumin adsorption from the top to the interface with phospholipid monolayer, to understand their dynamics and the effects of the serum albumins on the phospholipids. It is known that the serum albumin in the blood inhibits the lung surfactant and leads to respiratory failure, but it was never investigated before that what happen if albumin in blood flow directly to the alveoli surface through airway, not from the aqueous alveolar fluid. When the droplet of the serum albumin coalesces with phospholipid monolayer, the serum albumin leaves a ‘scar’ at the interface. This scar-like layer has radial fingering patterns, and it is very stable. The structures are not completely disappeared for more than 3 hours even above 20 mN/m, the equilibrium surface pressure of the albumin. The stable albumin layers are observed when the albumin concentration is above 0.05 mg/ml, and it is very critical value to patients considering that ~40 mg/ml of albumin is in the blood.