Repairing Damaged Hair Using Pentapeptides of Various Amino Acid Sequences with Crosslinking Reaction

Choi, Wonkyung,Son, Seongkil,Song, Sang-Hun,Kang, Nae Gyu,Park, Sun-gyoo Society of Cosmetic Scientists of Korea 2020 Korean journal of cosmetic science Vol.2 No.1

The aim of this study is to investigate the effect of various pentapeptides on hair repair depending on the characteristics of comprising amino acids using crosslinking agents in hair. Total ten peptides were synthesized with two kinds of amino acids respectively, of which were previously categorized according to R group of the amino acids contributing to the characteristic of each peptide: STTSS (Ser-Thr-Thr-Ser-Ser), LIILL (Leu-Ile-Ile-Leu-Leu), CMMCC (Cys-Met-Met-Cys-Cys), DEEDD (Asp-Glu-Glu-Asp-Asp), RKKRR (Arg-Lys-Lys-Arg-Arg), TAMRA-STTSS, TAMRA-LIILL, TAMRA-CMMCC, TAMRA-DEEDD, and TAMRA-RKKRR. Pentapeptide alone, or pentapeptides with crosslinking agents such as polymeric carbodiimide (PCI) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were treated to chemically damaged hair. Hair diameter and break strength (N = 40/case) were measured to calculate tensile strength of hair for computing hair repair ratio, and fluorescence yields (N = 20/case) were collected for hair treated with TAMRA-peptides. The tensile strength of hair treated with pentapeptides alone, or pentapeptides with cross-linking agents is consistent with the fluorescence yield from the microscope images of the cross-sectioned hair in vision and in numerical values. Pentapeptides consisting of hydrophobic amino acids (LIILL), amino acids with sulfur (CMMCC), and basic amino acids (RKKRR) increased the tensile strength in perm-damaged hair. Pentapeptides with no extra carboxyl/amine groups in R group of amino acids resulted in no significant differences in hair strength and fluorescence yield among hairs treated with alone and with crosslinkers. Pentapeptides with extra carboxyl groups or amine groups enabled further strengthening of hair due to increased bonds within the hair after carbodiimide coupling reaction. The hair repairs of pentapeptides with various amino acid sequences were studied using crosslinking. Depending on the physical characteristics of comprising amino acids, the restoration of damaged hair was observed with tensile strength of hair and fluorescence signals upon cross-sectioned hair in parallel to possibly understand the binding tendency of each pentapeptide within the hair.

인체모낭 기관배양 시 모낭의 형태와 성장속도에 영향을 미치는 배양인자에 관한 연구

김윤성 ( Yoon Sung Kim ),김경훈 ( Kyung Hoon Kim ),이우재 ( Woo Jae Lee ),정지원 ( Ji Won Jung ),송계용 ( Kyae Yong Song ),서기범 ( Ki Beom Suhr ),이증훈 ( Jeung Hoon Lee ),박장규 ( Jang Kyu Park ) 대한피부과학회 1998 대한피부과학회지 Vol.36 No.2

Background: In order to study hair biology, a hair organ culture system is necessary. However satisfactory hair culture systems have not been established. Objective : The purpose of this study was to examine the effectiveness of growth factors and to establish a hair organ culture system for studying hair biology and to evaluate the effectiveness of growth factors. Method: After the healthy human anagen hair follicles were collected without any visible damage, they were cultured in William E medium with several combinations of growth factors including insulin, hydrocortisone, sodium selenite, human transfemn, fetal calf serum and epidermal growth factor at 37C in an atmosphere of 5% CO2/air incubation. The culture medium was changed every 3 days. The results were evaluated by measuring hair growth and hair follicle morphology. Results : The results of this study are summarized as follows; 1) In the medium composed of insulin, hydrocortisone,sodium selenite and human transferrin, the human hair follicles continued to grow at an in vivo rate of 0.3mm in a day over 10 days without change of gross and microscopic morphology. 2) In the medium containing insulin and/or hydrocortisone the growing rate of the human hair follicles was similar to that in vivo, but the follicles revealed premature entry into catagen at 2-6 days in the culture macroscopically and microscopically. 3) Adding fetal calf serum to the above medium made the hair follicles retain the freshly isolated hair follicles morphology for 10 days in culture, even though they grew somewhat slower than the in vivo rate from 6 days in culture. 4) The effectiveness of EGF mimics the in vivo depilation of EGF in sheep. Conclusion : To supplement insulin, hydrocortisone, sodium selenite, transferrin as growth factors, William E medium was necessary for maintenance of an in vivo growth rate and the morphology the anagen hair follicles. This culture system is not enough, but it might be useful for investigation of the physiology, biology of hair follicles as well as pharmacology and toxicology in hair. (Korean J Dermatol 1998;36(2): 210-216)

In vivo and In vitro hair growth promotion effects of extract from Glycine soja Siebold et Zucc

( Jae Chan Yang ),( Bo Ae Kim ) 한국응용생명화학회 2016 Journal of Applied Biological Chemistry (J. Appl. Vol.59 No.2

Hair is a dermal adjunctive organ that protects the body from external physical and chemical stimuli; hair undergoes anagen, catagen, and telogen phases, with hair-loss occurring during the telogen phase. Alopecia is a condition wherein a person undergoes hair-loss far exceeding the normal amount, owing to diverse external factors. Wild beans are rich in isoflavone and amino acids known to prevent hair-loss; compared to cultivated beans, many wild bean species have higher protein content. This study aimed to develop a hair growth promoting solution, with superior hair growth promoting effects and fewer side effects, using naturally obtained Glycine soja Siebold et Zucc (GSSZ) extracts. Sevenweek- old C57BL/6N male mice were classified into different experimental groups. Hair growth was observed in GSSZ-treated mice, and compared against that seen in 3 % minoxidil (MXD, positive control)-treated mice. Visual observations revealed a greater reduction in hair-loss in MXD and GSSZ application groups, compared to that in TXN group (hair loss induction using 1 % testosterone). Evaluation using an image analysis software revealed that compared to the positive control, TXN + GSSZ group showed the highest hair growth. TXN + MXD and control groups exhibited similar follicular cell growth, while the hair growth promotion patterns were similar in the negative control (normal), TXN + GSSZ, and TXN groups, as observed via histological analysis. GSSZ did not induce cytotoxicity (even at 2 mg/mL) in keratinocytes and dermal papilla cells; alternately, dermal papilla cell proliferation was activated in a (GSSZ) concentration-dependent manner. Therefore, the GSSZ extract promoted hair growth and increased hair growth-related cell activity, and could therefore be utilized in alopecia treatment.

Hair Pack과 LPP 제품의 처리가 손상모발에 미치는 영향

신임순 ( Im-soon Shin ) 한국미용예술경영학회 2015 미용예술경영연구 Vol.9 No.4

Hair treatments with hair pack and LPP types were repeatedly processed for 3 times and permanent was repeated consecutively for 3 times. And changes in the cuticle layer were observed using an electron microscope to find the effects of hair treatments on the damaged hair. Then, elongation and tensile strength were measured and the following results were obtained. First, the control group with no hair treatments showed a mild degree of cuticle status but the cuticle was extremely unstable with almost half of it falling off. In hairs with 2 and 3 consecutive treatments with permanent showed loss of most cuticle layers and some of them even had holes. Especially, the 3 time treatment group showed extremely severe damage with exposure of cortex from cracks. Second, cuticle layer was somewhat maintained on the hair surface after one time permanent operation with hair pack processing. However, cuticle layers were not clearly seen after second and third permanent operations with damages such as holes. Third, cuticle layer after one time permanent operation with LPP processing was maintained. Looking at the surface of hair with 3 repeated processing of LPP, observation of damage was not observed at low magnification but rough and split surface was found in high magnification, different from first and second processing. Fourth, However, tensile strength and elongation after 1, 2 and 3 consecutive permanent operations with a hair pack and LPP processing were found to be 199.9, 102.2, 102.7 and 58.0, 58.4, 61.1, and 101.4, 100.2, 100.0 and 60.6, 59.8, 58.0.

염색(染色) 후(後) 경시적(經時的)인 모발(毛髮)의 형태변화(形態變化)와 염색(染色) 영향인식(影響認識)

이진옥 ( Jin Ok Lee ),이주영 ( Ju Young Lee ) 한국미용학회 2008 한국미용학회지 Vol.14 No.2

This study wished to search about shape changing of hair and recognition of hair-coloring effect after hair-coloring treatment. First, in view of a result after measuring changes of hair thickness before and after the hair-coloring of normal hair, average thickness of measured object hair was increased, but there was not any special difference in statistical data in comparing before hair-coloring with after 2 and 4 weeks. Second, in observing cuticles with SEM, each scale layer in the hair cuticle layer before the hair-coloring treatment was adhered closely in regular space. Third, in observing cuticles with TEM, it could be seen border membrane of cuticle layer and cuticle layer became clear border without any damage in the normal hair before the hair-coloring treatment. Forth, analysis showed that five causes were special factors of hair loss people who had experienced the hair-coloring in test as a result of multi logistic recurrence analysis.

염모제의 성분 특성 및 사용횟수에 따른 모발손상도 연구

김경진(Gyeung-Jin Kim),강민숙(Min-Sook Kang),문덕환(Deog-Hwan Moon) 한국인체미용예술학회 2011 한국인체미용예술학회지 Vol.12 No.4

This study was conducted to assess the level of hair damage according to type of hair dye components used and to prepare fundamental data for promoting healthy hair. Samples came from a 14-year-old girl whose hair had never been dyed. It was cut into 20cm lengths at a point 5㎝ away from the scalp. Each sample weighed 2g. The hair samples were dyed once, twice, and three times with eight kinds of hair dyes and dried naturally. The cuticles were observed with SEM to examine hair damage. To evaluate the damage of hair observed with SEM, 5 levels of damage were classified. Ten professors of hairdressing in the Department of Cosmetology evaluated the samples. There were significant differences in hair damage according to the number of times the hair was dyed. The level of damage was greatest in hair dyed with chemical henna and lowest in hair dyed with the squid ink.

Analysis of pubic hair as an alternative specimen to scalp hair: A contamination issue

Lee, S.,Han, E.,In, S.,Choi, H.,Chung, H.,Chung, K.H. Elsevier Sequoia 2011 Forensic Science International Vol. No.

Pubic hair is often analyzed as an alternative to scalp hair to prove previous drug use. However, urine is a potential source of external contamination. In the present study, the concentrations of methamphetamine (MA) and amphetamine (AP) in both scalp and pubic hair from illegal MA users were compared. Furthermore, in order to investigate the external contamination of pubic hair by urine, MA and AP absorbed into pubic hair that had been contaminated with authentic urine from a MA user were measured using a previously validated method. The effect of shampoo-wash on the contaminated pubic hair was also examined. However, no correlation was found in the MA and AP concentrations between scalp and pubic hair from illegal MA users. As the number of contamination events by authentic urine increased, the concentrations of MA and AP in pubic hair increased. Both MA and AP were detected in the first methanol washes of the contaminated hair samples but were not detected in the second methanol washes. As the number of shampoo-washes of the contaminated pubic hair increased, the concentrations of MA and AP gradually decreased. Even though pubic hair can be used as an alternative to scalp hair to prove previous drug use, it should be avoided when estimating drug use history. It should be also noted that higher quantitative results in pubic hair do not necessarily represent heavier drug use.

Hair-dyeing Effects and Changes in Hair Texture by Arecae Semen Pigment

Ju-Sub Kim 한국피부과학연구원 2021 아시안뷰티화장품학술지 Vol.19 No.1

목적: 본 연구는 빈랑자 염료로 모발에 도포하여 염색효과와 모질 특성 변화를 알아보고자 하였다. 방법: 빈랑자 염료를 중탕하여 사용하였다. 염료를 건강모와 탈색한 시료 모발에 도포 후 열처리와 자연방치 시간을 다르게 한 후 시료별로 도포 전과 후를 측정 하여 비교 분석하였다. 염색효과를 알기 위해 색차계를 이용하여 L*, a*, b* 값과 물 빠짐을 측정하였다. 또한 모질 특성 변화를 알 기 위해 인장강도, 메틸렌블루를 이용한 흡광도, 광택을 측정하였다. 결과: 염색효과 측정 결과는 L*값은 건강모에서는 변화가 없고 7L(2)과 7L(3), 9L(3)에서는 염색 효과가 있었다. a*값과 b* 값은 도포 전과 후의 통계분석 결과 건강모는 변화가 없음을 알 수 있었 고, 7L(1)과 7L(2), 7L(3), 9L(3)은 유의미한 결과로 염색 효과가 있었다. 물 빠짐 측정결과는 1일차에는 가장 많이 빠졌고, 3일차부 터는 더 이상의 물 빠짐이 없었다. 인장강도 측정결과는 7L(1)과 7L(2), 7L(3), 9L(3) 시료에서 통계분석 결과 모질의 특성 변화는 없 었다. 메틸렌블루를 이용한 흡광도 분석결과 건강모와 7L(1)과 7L(2), 7L(3), 9L(3) 시료에서 통계분석 결과 모질의 특성 변화는 없 었다. 광택 측정결과는 통계분석 결과 광택에 변화를 주는 것을 알 수 있었다. 결론: 빈랑자 염료에 의한 모발 염색효과가 있었고, 모질의 특성 변화는 광택은 있고, 인장강도와 흡광도 변화는 없었다. 차후 다양한 염색 처리방법에 의한 염색효과 연구와 결과의 신 뢰성 확보를 위한 시료의 측정 회수를 늘릴 필요가 있다. Purpose: This study examines hair-dyeing effects and changes in the characteristics of hair texture by applying Arecae semen pigment to hair. Methods: The Arecae semen pigment was double-boiled and applied to two samples, namely, healthy and bleached hair. The samples were heat-treated and left in natural condition for different hours. The samples were measured before and after application and comparatively analyzed. To quantify the hair-dyeing effects, the L*, a*, and b* values as well as fading were measured using a color-difference meter. Additionally, tensile strength, absorbance using methylene blue, and gloss were measured to examine changes in the characteristics of hair texture. Results: The results indicated no changes in the L* value for healthy hair, whereas hair-dyeing effects were observed for 7L(2), 7L(3), and 9L(3). Analysis of a* and b* values revealed that healthy hair remained the same, whereas significant hair-dyeing effects were noted for 7L(1), 7L(2), 7L(3), and 9L(3). In terms of fading, the most notable fading occurred on Day 1, whereas none was noted on Day 3. Samples 7L(1), 7L(2), 7L(3), and 9L(3) were used to test for tensile strength, where no changes in hair texture were observed. Furthermore, analysis was conducted on healthy hair and samples 7L(1), 7L(2), 7L(3), and 9L(3) after measuring absorbance using methylene blue. No changes in hair texture were noted. Lastly, the hair samples exhibited no changes after measuring gloss. Conclusions: The study confirmed the hair-dyeing effects of the Arecae Semen pigment. According to hair texture, changes were observed for gloss, whereas tensile strength and absorbance remained the same. In the future, conducting the study on larger samples is necessary to ensure the reliability of results and research on various methods of hair dyeing.

Henna(Lawsonia intermis) 염색이 모발의 색도변화와 큐티클 형태 변화에 미치는 영향

박은준 ( Eun Jun Park ),김기영 ( Ki Young Kim ) 한국미용학회 2008 한국미용학회지 Vol.14 No.4

Continuous using of blaching agents and oxidative hair dyeing agents induced to damage of hair cuticle and cortex layer. In this study, color change and morphological change of cuticle were observed by henna (Lawsonia innermis) dyeing on hair. Prepared 5 hair pieces without normal hair piece were delcolored, acid colorant treated or henna treated, and observed lightness, redness and yellowness by measurement of chromo meter and morphological change of cuticle by observation of SEM. In the result, the diameter of bleached hair (6% hydrogen peoxide treated) was significantly thinner than normal hair, acid colorant incubated and henna incubated hairs(p<0.005), and normal and 1/3 hrs henna incubated hairs were 12 μm. The highest lightness was showed bleached hair and henna treated hairs were showed the highest redness and yellowness. Lightness, redness and yellowness is not dependent on reaction time by using of acid colorant and henna. Cuticle damage and microfilament bundle were revealed on bleached hair. Henna treated hairs were showed weak cuticle damage and no exposure of microfilament. And hair damage on acid colorant treated hairs was stronger than henna treated hairs. In conclusion, henna could be protect of cuticle and may be very good natural dye for hair coloring.

두개피 관리 의식도 조사

오강수,지정훈 한국패션뷰티학회 2006 한국패션뷰티학회지 Vol.4 No.3

Hair is the part of body which expresses man into more manlike. When we create some artworks, we need the most suitable material for the artworks. Similarity, the healthy scalp hair condition and treatment are required to keep our hair clean and healthy. And the improper condition of scalp hair prohibits our hair from normal growing, proceeds the alopecia(loss of hair) and double our physical and mental stresses. The necessity of health treatment of scalp hair which was damaged by frequent permanent hair wave and hair coloring is on the rise. This study will present how to control and treat the scalp hair and will check how we feel about the necessity of scalp hair treatment. This study finally shows that people think their scalp hair conditions are not normal and chemically treated hair in spite of their natural hair. However, most people think that the scalp hair treatment is very useful to their hair and this result enables us to predict the future market of the scalp hair treatment business will be growing day by day. We need to study further not only scalp hair treatment but also prevention stage in order to keep the healthy hair and will check how we feel about the necessity of scalp hair treatment.