Citrobacter sp.에 의한 Triphenylmethane계 색소의 분해

민상기,조영배,전홍기 부산대학교 유전공학연구소 1995 분자생물학 연구보 Vol.11 No.-

염색공장 폐수처리장의 폭기조로부터 분리한 Citrobacter sp. SK-3균주의 생육 특성을 검토하고, triphenylmethane계 색소중 염기성 염료로 사용빈도가 높은 crystal violet을 비롯한 pararosaniline, brilliant green등에 대한 분해능을 조사하였으며, crystal violet의 분해산물을 HPLC로 추정하였다. Citrobacter sp. SK-3균주의 triphenylmethane계 색소를 분해하기 위한 최적배지 조성은 최소 무기염배지에 탄소원으로서는 galactose 0.5%, 질소원으로서는 beef extract 0.1%, 초발 pH는 8.0-9.0 였으며, 최적배지에 crystal violet 200ppm을 첨가하여 40℃ 부근에서 배양하였을 때 24시간 이내에 100%의 분해율을 나타내었다. Citrobacter sp. SK-3균주는 triphenylmethane계 색소중 crystal violet뿐만 아니라 malachite green, pararosaniline, brilliant green, methyl violet, basic fuchsin 등과, Azo계 색소인 methyl red를 분해하였다. Citrobacter sp. SK-3균주는 무기염 배지상에서 crystal violet, brilliant green을 유일한 탄소원, 질소원으로 이용하여 생육 가능하였다. Citrobacter sp. SK-3균주에 의한 crystal violet 분해산물을 TLC 및 HPLC로서 분석한 결과, crystal violet의 기본골격에 결합된 methyl기가 점차 demethylation되어 pentamethyl-, tetramethyl-, trimethyl- pararosaniline등의 물질들로 분해되어짐을 확인할 수 있었다. The optimal condition for degradation of crystal violet and other triphenylmethane dyes by Citrobacter sp. SK-3 isolated from the activated sludhe of dye manufacturing factory was investigated. The optimal culture medium for the degradation of triphenylmethane dye was composed of minimum inorganic salt medium supplemented with 0.5% galactose, 0.1% beef extract, with the initial pH of 8.0 to 9.0. Under this condition, Citrobacter sp. SK-3 degraded 200ppm of crystal violet completely within 24 hours. Citrobacter sp. SK-3 also degraded efficiently malachite green, pararosaniline, brilliant green, methyl violet, basic fuchsin and methyl red. Analysis of the degradation products of crystal violet through thin layer chromatography and high performance liquid chromatography indicated that the methyl groups bound to crystal violet backborn were gradually demethylated to pentamethyl-, tetramethyl- and trimethylpararosaniline.

목질계 활성탄에 의한 수중의 Methylene blue와 Crystal violet의 제거 특성

전진우 ( Jin Wo Jeon ),유해나 ( Hae Na Yu ),감상규 ( Sang Kyu Kam ),이민규 ( Min Gyu Lee ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.11

The adsorption ability of wood-based activated carbon to adsorb methylene blue (MB) and crystal violet (CV) from aqueous solution has been investigated. Adsorption studies were carried out on the batch experiment at different initial MB and CV concentrations (MB=150 mg/L~400 mg/L, CV=50 mg/L~350 mg/L), contact time, and temperature. The results showed that the MB and CV adsorption process followed the pseudo-second-order kinetic and intraparticle diffusion was the rate-limiting step. Adsorption equilibrium data of the adsorption process fitted very well to both Langmuir and Freundlich model. The maximum adsorption capacity (qm) by Langmuir constant was 416.7 mg/g for MB and 462.4 mg/g for CV. The thermodynamic parameters such as ΔH°, ΔS° and ΔG° were evaluated. The MB and CV adsorption process was found to be endothermic for the two dyes.

Counterion-dye staining method for DNA in agarose gels using crystal violet and methyl orange

Yang, Yong Il,Jung, Da Woon,Bai, Dong Gyu,Yoo, Gyurng Soo,Choi, Jung Kap 전남대학교 약품개발연구소 2001 약품개발연구지 Vol.10 No.-

Sensitive and safe methods for visualization of DNA in agarose gels are described. 0.001 % crystal violet dissolved in distilled water was used for DNA staining on agarose gels and it could detect as little as 16 ng of DNA (3 kb, pGem-7Zf/EcoRl) without destaining procedure. The detection limit is four times lower than that of ethidium bromide. To improve the sensitivity, we studied a counterion-dye staining method using methyl orange as a counterion-dye which contributes to reduce excessive background staining by crystal violet. Dye concentration, pH of staining solution, mixing molar ratio of two dyes, and staining times were optimized for the counterion-dye staining. By the staining with a mixed solution of 0.0025% crystal violet and 0.0005% methyl orange in distilled water, 8 ng of the 3 kb DNA in an agarose gel was detected within 30 min.

저농도 인산 이온의 정량법 개발에 관한 연구

정형근,구현빈,신용승 연세대학교 보건과학연구소 1999 보건과학논집 Vol.9 No.-

Crystal violet as CMOS-compatible alkali-free promoter for CVD growth of MoSe2 monolayers: Comparative surface analysis with alkali-based promoter

김수진,Gwang Hwi An,최민,Kang Yae Zy,Kim Tae Yeon,Rahman Ikhwan Nur,방준혁,김경완,김동현,이현석 한국물리학회 2023 Current Applied Physics Vol.48 No.-

The wafer-level growth of transition metal dichalcogenides (TMDs) using chemical vapor deposition (CVD) is essential for industrial applications involving complementary metal-oxide-semiconductor (CMOS) processes. In the CVD processes of TMDs, promoters containing alkali elements such as Na and K are typically used to obtain high-quality crystals. However, the use of alkali elements is rigorously restricted in CMOS processes because of contamination problems. In this study, we investigated crystal violet (CV) as a promising alkali-free promoter in the CVD growth of MoSe2. Particularly, the surface properties were intensively examined by optical, chemical, and topographical analyses and compared to those of samples prepared with NaOH as a representative alkali-based promoter. Na residues causing a doping effect were observed in the NaOH process, whereas the doping effect was negligible in the CV process. After the transfer process from the as-grown state, the strain effect in the NaOH process was prominently changed, resulting in wrinkles on MoSe2, whereas the wrinkles were negligible in the CV process, suggesting relatively smaller strain accumulation in the CV process during crystal growth. Our results showed the feasibility of CV as an alkali-free promoter for utilizing CVD-grown TMDs in industrial CMOS processes.

Myricetin Disturbs the Cell Wall Integrity and Increases the Membrane Permeability of Candida albicans

( Heung-shick Lee ),( Younhee Kim ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.1

The fungal cell wall and membrane are the principal targets of antifungals. Herein, we report that myricetin exerts antifungal activity against Candida albicans by damaging the cell wall integrity and notably enhancing the membrane permeability. In the presence of sorbitol, an osmotic protectant, the minimum inhibitory concentration (MIC) of myricetin against C. albicans increased from 20 to 40 and 80 μg/ml in 24 and 72 h, respectively, demonstrating that myricetin disturbs the cell wall integrity of C. albicans. Fluorescence microscopic images showed the presence of propidium iodide-stained C. albicans cells, indicating the myricetin-induced initial damage of the cell membrane. The effects of myricetin on the membrane permeability of C. albicans cells were assessed using crystal violet-uptake and intracellular material-leakage assays. The percentage uptakes of crystal violet for myricetin-treated C. albicans cells at 1×, 2×, and 4× the MIC of myricetin were 36.5, 60.6, and 79.4%, respectively, while those for DMSO-treated C. albicans cells were 28.2, 28.9, and 29.7%, respectively. Additionally, myricetin-treated C. albicans cells showed notable DNA and protein leakage, compared with the DMSO-treated controls. Furthermore, treatment of C. albicans cells with 1× the MIC of myricetin showed a 17.2 and 28.0% reduction in the binding of the lipophilic probes diphenylhexatriene and Nile red, respectively, indicating that myricetin alters the lipid components or order in the C. albicans cell membrane, leading to increased membrane permeability. Therefore, these data will provide insights into the pharmacological worth of myricetin as a prospective antifungal for treating C. albicans infections.

Adsorption of Crystal Violet from Wastewater by Modified Bambusa Tulda

Nirban Laskar,Upendra Kumar 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.8

In the present study sodium carbonate modified Bambusa tulda was utilised for the removal of crystal violet dye from aqueoussolution. The functional group characterization and the surface morphology was done by Fourier Transform Infrared Spectroscopy(FTIR) and Scanning Electron Microscope (SEM). It confirms the hydroxyl groups and carboxyl group present on the surface ofmodified Bambusa tulda. The optimum condition for the removal of crystal violet was taken place at pH 7, 200 rpm, dose at 10gm/l,initial concentration 50 mg/l, at equilibrium time 60 minutes and 298 K temperature with maximum adsorption capacity of 20.84 mg/gm. The adsorption of crystal violet by modified Bambusa tulda best fits in Langmuir isotherm model with R2 value 0.924 andPseudo 2nd order rate equation model with R2 value of 0.999. Other parameters like isosteric heat analysis, thermodynamics profileand activation energy were investigated. Thus, modified Bambusa tulda can be an efficient and economically used as an alternativefor activated carbon for the removal of crystal violet from waste water.

활성탄에 의한 Crystal Violet 흡착에 있어서 흡착동력학, 열역학 인자 및 등량흡착열

이종집 ( Jong Jib Lee ) 한국공업화학회 2017 공업화학 Vol.28 No.2

활성탄을 사용하여 수용액으로부터 crystal violet 염료의 흡착에 대해 조사하였으며, 흡착제의 양, 초기농도와 접촉시간 및 온도를 흡착변수로 사용하여 수행하였다. 흡착평형관계는 Langmuir 등온식에 잘 맞았다. 평가된 Langmuir 분리계수(R_L = 0.02~0.106)를 바탕으로 이 흡착공정이 효과적인 처리(0 < R_L < 1)가 가능하다는 것을 알았다. 흡착동력학 데이터는 유사 2차 반응속도식에 잘 맞는 것으로 나타났다. Gibbs 자유에너지(-1.61~-11.66 kJ/mol)와 엔탈피(147.209kJ/mol)는 흡착공정이 자발적이고 흡열반응으로 진행된다는 것을 나타냈다. 등량흡착열은 표면덮임이 증가됨에 따라 흡착제-흡착질의 상호작용이 제한되어 표면부하량이 증가할수록 작아졌다. The adsorption of crystal violet dyes from aqueous solution using the granular activated carbon was investigated. Adsorption experiments were carried out as a function of the adsorbent dose, initial concentration, contact time and temperature. The adsorption characteristic of crystal violet followed Langmuir isotherm. Based on the estimated Langmuir separation factor (R_L = 0.02~0.106), this process could be employed as an effective treatment (0 < R_L < 1). The adsorption kinetics followed the pseudo second order model. The values of Gibbs free energy (-1.61~-11.66 kJ/mol) and positive enthalpy (147.209 kJ/mol) indicated that the adsorption process is a spontaneous and endothermic reaction. The isosteric heat of adsorption decreased with increasing of surface loading by the limited adsorbent-adsorbate interaction due to increased surface coverage.

Efficient removal of crystal violet and eosin B from aqueous solution using Syzygium cumini leaves: A comparative study of acidic and basic dyes on a single adsorbent

Arshad Mehmood,Sheher Bano,Aisha Fahim,Riffat Parveen,Shazia Khurshid 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.5

The adsorption capabilities of Syzygium cumini leaves were investigated for crystal violet and eosin B using batch adsorption method. Removal conditions were optimized by varying operational parameters like pH, dose of adsorbent, contact time and temperature. Presence of salts had a profound effect on the adsorption and the experimental data for both adsorbates, providing good correlation with the Temkin, Langmuir and Freundlich patterns, but differing from Dubinin-Radushkevich model. Maximum adsorption capacity was found to be 38.75 mg/g for crystal violet and 16.28mg/g for eosin B respectively. Boyd-Adamson-Myers, Morris-Weber and Bangham’s surface mass transport models revealed that film diffusion was the rate controlling process and followed pseudo-second order kinetics. Activation energy was estimated to be 57.265 and 6.721 kJ/mol for crystal violet and eosin B respectively. Adsorption of crystal violet is endothermic and that of Eosin B is exothermic but both were spontaneous at all temperatures. To study the bulk removal of the dyes, column operations were made. The exhausted columns were regenerated by eluting HCl solution and almost 91.94% of CV and 58.08% of EB were recovered from columns, respectively.

Removal of Crystal Violet Dye from Textile Wastewater by Using Rice Husk As Low Cost Adsorbent

( Phyo Phyo Kyi ),( Seong-jik Park ) 한국농공학회 2018 한국농공학회 학술대회초록집 Vol.2018 No.-

Textile industry significantly contributes to the rapid economic growth rate of any countries. On the other sides, the textile industry uses a large amount of organic and inorganic chemicals as dyes that are directly or indirectly responsible for producing wastewater. The colored wastewater discharged from textile industries causes water pollution problems and it has been becoming one of the environmental concerns nowadays. The objective of this investigation was to explore the adsorption potential of rice husk (RH), as a low-cost adsorbent for the removal of Crystal Violet (CV) dye from textile wastewater. A single stage batch adsorber was designed to obtain the optimum condition for Crystal Violet (CV) dye removal. Equilibrium and kinetics studies were carried out to evaluate the adsorption of Crystal Violet (CV) onto rice husk. According to the equilibrium assessment, the adsorption data were best fitted with Freundlich isotherm. The adsorption kinetics followed the pseudo-second-order kinetic model. The results of this study indicate that rice husk is found to be a cost-effective and an efficient adsorbent for the removal of CV from textile wastewater.