Ti-6Al-7Nb 합금의 표면 활성에 미치는 양극산화 공정조건의 영향

권오성,류경호,박광범,이민호,배태성,이오연 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.2

This study was performed to investigate whether the bioactivity of the anodized and hydrothermally treated Ti-6A1-7Nb alloy were affected by the process conditions of anodic oxidation. Anodizing was performed at a current density of 5-50 mA/㎠ in up to 240~300 V in electrolyte solutions containing DL-α-glycerophosphate(DL-α-GP) and calcium acetate (CA). Hydrothermal treatment was done at 300℃ for 2 hours to produce hydroxyapatite (HA) for bioactivity. Bioactivity was evaluated from surface activation layer formed on the surfaces in a Hanks' solution with pH 7.4 at 36.5℃ for 30 days. The size of micropores and the thickness of oxide film were increased by increasing the spark forming voltage, and more complex oxide layers were formed. HA crystals were observed on the anodic oxide film after hydrothermal treatment at 300℃ for 2 hours. The anodic oxide films were composed of strong anatase peak and rutile peak, and showed the increase in intensity of anatase peak after hydrothermal treatment. When electrolyte composition, voltage and current density were increased, the deposited HA crystals showed a fine shapes. Bioactivity in Hanks' solution was accelerated when the oxide films were composed of strong anatase peak with rutile peak. Oxide layers selectively showed surface activity at the pertinent electrolyte composition. When voltage and current density were increased, the surface activity was increased. The amount of Ca and P was increased in surface having bioactivity in Hanks' solution. (Received December 1, 2004)

국내 전통주 주박의 이용과 유용생리활성

김미선(Mi-Sun Kim),신우창(Woo-Chang Shin),손호용(Ho-Yong Sohn) 한국생명과학회 2015 생명과학회지 Vol.25 No.9

발효 폐기물의 대부분을 차지하는 주박은 발효 후 알코올성 발효액을 필터, 원심분리 또는 증류 후에 얻어지는 찌꺼기 및 1차 제조된 제품의 숙성과정 중에 생성되는 찌꺼기를 말한다. 국내 전통주 주박은 식용 원재료와 다양한 약용작물을 첨가하고 이를 누룩으로 발효시켜 제조하여, 안전성이 확보되면서 생리활성이 우수한 장점을 가지고 있다. 최근의 탁주 위주의 전통주 시장 성장으로 주박 생산량은 빠르게 증가되고 있으며, 이의 폐기물 처리는 더욱 엄격해지고 있으나, 국내에서는 아직 전통주 주박의 효율적인 재이용에 대한 연구는 제한적이다. 본 총설에서는 전통주 주박의 산업동향, 연구동향 및 특허동향을 분석하고, 전통주 주박의 유용생리활성물질들을 제시하였다. 전통주 주박은 단순 발효폐기물이라는 인식에서 벗어나, 식량, 식품첨가물, 비료, 사료, 미생물 배양원, 기능성 식품소재, 향장 및 미용소재의 고부가가치 발효소재 공급원이라는 인식의 전환이 필요하며, 향후 재처리 기술, 유용물질 회수기술 개발을 위한 체계적인 산학관 연계와 연구가 필요하다. The lees of Korean traditional wine called as Jubak or Sul-jigemi in Korea is byproduct from alcohol fermentation industry, which is remnant of fermentation broth after filtration, centrifugation, distillation, or sedimentation during aging. Since, Korean traditional wines are produced from edible plant sources such as rice, foxtail millet, fruits and medicinal herbs with nuruk (a traditional fermentation starter and starch degrader), the Jubak from rice wine (takju), medicinal herb wine (yakju) and fruits wine are considered as safe byproduct and have various useful bioactivity. Considering the recent rapid increased production of Jubak from takju industry, and the reinforcement of dispose of Jubak as waste material in worldwide, the development of efficient reuse process for Jubak is necessary in traditional wine industry. In this review, the status of current industry, research and patent trends in relation with Jubak production, treatment, utilization and renewal was analyzed and different bioactive compounds including phenolic acids from Jubak were provided. Jubak is not any more waste material, and is the source of bioactive functional materials for food, cosmetics and medicinal industry. To develop the efficient and economic renewal technology including recovery process for bioactive substances from Jubak, systematic collaboration and research among the industry, academy and government is necessary.

Bioactivities of the edible brown seaweed, <i>Undaria pinnatifida</i>: A review

Wang, Lei,Park, Young-Jin,Jeon, You-Jin,Ryu, BoMi Elsevier 2018 Aquaculture Vol.495 No.-

<P><B>Abstract</B></P> <P>Natural ingredients containing high nutrients, various natural compounds, with broad spectrum of bioactivities and wide range of natural distribution or production, have been attracting more and more attention in food and pharmaceutical industries. <I>Undaria pinnatifida</I>, is an edible brown seaweed that contains high carbohydrate, protein, microelement, and various types of secondary metabolites, which possess strong bioactivities. It has been one of the popular seaweeds used for food as well as medicine in most of Asian countries including China, Korea, and Japan for a long time. Recent studies suggest that <I>U. pinnatifida</I> is rich in natural compounds such as polyphenols, peptides, and polysaccharides, these compounds possess various bioactivities such as anticancer, antioxidant, anti-inflammatory, and anti-diabetes properties. In the present review, we have collected and summarized the scientific data published about the bioactivities of <I>U. pinnatifida</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The chemical components of the edible brown seaweed, <I>Undaria pinnatifida</I> had been summarized. </LI> <LI> The bioactivities of the edible brown seaweed, <I>Undaria pinnatifida</I> had been summarized. </LI> <LI> Recent bioactivities research progress of the edible brown seaweed, <I>Undaria pinnatifida</I> had been discussed. </LI> </UL> </P>

Metabolic Profiling and Biological Activities of Bioactive Compounds Produced by Pseudomonas sp. Strain ICTB-745 Isolated from Ladakh, India

( Kamal ),( Ahmed ),( Anver Basha Shaik ),( C. Ganesh Kumar ),( Poornima Mongolla ),( P. Usha Rani ),( K. V. S ),( Rama Krishna ),( Suman Kumar Mamidyala ),( Joveeta Joseph ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1

In an ongoing survey of the bioactive potential of microorganisms from Ladakh, India, the culture medium of a bacterial strain of a new Pseudomonas sp., strain ICTB-745, isolated from an alkaline soil sample collected from Leh, Ladakh, India, was found to contain metabolites that exhibited broad-spectrum antimicrobial and biosurfactant activities. Bioactivity-guided purification resulted in the isolation of four bioactive compounds. Their chemical structures were elucidated by 1H and 13C NMR, 2D-NMR (HMBC, HSQC, 1H,1H-COSY, and DEPT- 135), FT-IR, and mass spectroscopic methods, and were identified as 1-hydroxyphenazine, phenazine-1-carboxylic acid (PCA), rhamnolipid-1 (RL-1), and rhamnolipid-2 (RL-2). These metabolites exhibited various biological activities like antimicrobial and efficient cytotoxic potencies against different human tumor cell lines such as HeLa, HepG2, A549, and MDA MB 231. RL-1 and RL-2 exhibited a dose-dependent antifeedant activity against Spodoptera litura, producing about 82.06% and 73.66% antifeedant activity, whereas PCA showed a moderate antifeedant activity (63.67%) at 60 μg/cm2 area of castor leaf. Furthermore, PCA, RL-1, and RL-2 exhibited about 65%, 52%, and 47% mortality, respectively, against Rhyzopertha dominica at 20 μg/ml. This is the first report of rhamnolipids as antifeedant metabolites against Spodoptera litura and as insecticidal metabolites against Rhyzopertha dominica. The metabolites from Pseudomonas sp. strain ICTB-745 have interesting potential for use as a biopesticide in pest control programs.

Preparation and characterization of bioactive calcium silicate and poly(ε-caprolactone) nanocomposite for bone tissue regeneration

Wei, Jie,Heo, S. J.,Liu, Changsheng,Kim, D. H.,Kim, S. E.,Hyun, Y. T.,Shin, Ji-Wang,Shin, Jung-Woog Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of biomedical materials research. Part A Vol.a90 No.3

<P>A novel biocomposite of nanosized calcium silicate (n-CS) and poly(ε-caprolactone) (PCL) was successfully fabricated directly using n-CS slurry, not dried n-CS powder, in a solvent-casting method. The in vitro bioactivity of the composite was evaluated by investigating the apatite-forming ability in simulated body fluid. A proliferation assay with mouse L929 fibroblasts was used to test the in vitro biocompatibility. The composition, hydrophilicity, and mechanical properties were also evaluated. Results suggest that the incorporation of n-CS could significantly improve the hydrophilicity, compressive strength, and elastic modulus of n-CS/PCL composites, with the enhancements mainly dependent on n-CS content. The n-CS/PCL composites exhibit excellent in vitro bioactivity, with surface apatite formation for 40% (w/w) n-CS (C40) exceeding that of 20% (w/w) n-CS (C20) at 7 and 14 days. The Ca/P ratios of apatite formed on C20 and C40 surfaces were 1.58 and 1.61, respectively, indicating nonstoichiometric apatite with defective structure. Composites demonstrated significantly better cell attachment and proliferation than that of PCL alone, with C40 demonstrating the best bioactivity. The apatite layers that formed on the composite surfaces facilitated cell attachment (4 h) and proliferation during the early stages (1 and 4 days). Collectively, these results suggest that the incorporation of n-CS produces biocomposites with enhanced bioactivity and biocompatibility. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009</P>

Identification of Neuroactive Constituents of the Ethyl Acetate Fraction from Cyperi Rhizoma Using Bioactivity-Guided Fractionation

( Yeomoon Sim ),( Jin Gyu Choi ),( Pil Sung Gu ),( Byeol Ryu ),( Jeong Hee Kim ),( Insug Kang ),( Dae Sik Jang ),( Myung Sook Oh ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.4

Cyperi Rhizoma (CR), the rhizome of Cyperus rotundus L., exhibits neuroprotective effects in in vitro and in vivo models of neuronal diseases. Nevertheless, no study has aimed at finding the neuroactive constituent(s) of CR. In this study, we identified active compounds in a CR extract (CRE) using bioactivity-guided fractionation. We first compared the anti-oxidative and neuroprotective activities of four fractions and the CRE total extract. Only the ethyl acetate (EA) fraction revealed strong activity, and further isolation from the bioactive EA fraction yielded nine constituents: scirpusin A (1), scirpusin B (2), luteolin (3), 6’-acetyl-3,6-diferuloylsucrose (4), 4’,6’ diacetyl-3,6-diferuloylsucrose (5), p-coumaric acid (6), ferulic acid (7), pinellic acid (8), and fulgidic acid (9). The activities of constituents 1-9 were assessed in terms of anti-oxidative, neuroprotective, anti-inflammatory, and anti-amyloid-β activities. Constituents 1, 2, and 3 exhibited strong activities; constituents 1 and 2 were characterized for the first time in this study. These results provide evidence for the value of CRE as a source of multi-functional neuroprotectants, and constituents 1 and 2 may represent new candidates for further development in therapeutic use against neurodegenerative diseases.

CaO-SiO₂-B₂O₃계 결정화 유리의 제조와 in-vitro법을 이용한 생체활성 평가

류현승,서준혁,김환,홍국선,김득중,이재협,이동호,장봉순,이춘기,Ryu, Hyun-Seung,Seo, Jun-Hyuk,Kim, Hwan,Hong, Kug-Sun,Kim, Deug-Joong,Lee, Jae-Hyup,Lee, Dong-Ho,Chang, Bong-Soon,Lee, Choon-Ki 한국세라믹학회 2002 한국세라믹학회지 Vol.39 No.5

$CaO-SiO_2-B_2O_3$계의 결정화유리의 소결 특성 및 기계적 특성, 생체활성을 알아보았다. 이 조성의 결정화유리는 750-830${\circ}$ 사이에서 소결 되었으며 치밀한 미세구조를 보였다. 이 결정화 유리는 단사정계 월라스토나이트(monoclinic wollastonite), 칼슘보레이트(Calcium borate, $CaB_2O_4$) 결정상과 보로실리케이트(borosilicate) 유리 기지상(matrix)의 3상으로 구성되었다. 기계적 강도는 지금까지 알려진 다른 생체활성 세라믹스보다 우수하였으며 특히 압축강도(2813 MPa), 파괴인성($3.12 MPa{\cdot}m^{1/2}$)이 높았다. 생체활성은 결정화유리 중 calcium borate와 보로실리케이트 유리의 양에 의존하였는데, 용해도가 높은 calcium borate는 의사체액(SBF)의 칼슘이온의 과포화도를 상승시키고 borosilicate 유리는 탄산아파타이트 핵생성에 필요한Si-OH기를 형성시켜 탄산아파타이트 층이 빨리 생성되기 때문으로 판단된다. 따라서 $CaB_2O_4$와 borosilicate 유리가 많을수록 결정화유리의 생체활성이 뛰어난 것으로 생각된다. Sintering property, mechanical property and bioactivity of $CaO-SiO_2-B_2O_3$ glass-ceramics were investigated. This glass-ceramics was sintered at 750-830${\circ}$ and showed nearly pore-free microstructure. The glass-ceramics consisted of three phases, i.e. monclinic-wollastonite, calcium borate and borosilicate glass matrix. The mechanical strength was higher than that of other bioactive ceramics, especially compressive strength(2813 MPa) and fracture toughness($3.12 MPa{\cdot}m^{1/2}$). Bioactivity of the glass-ceramics depends on amount of $CaB_2O_4$ and borosilicate glass matrix. It might be likely that more soluble $CaB_2O_4$ raises supersaturation of Ca ion in SBF solution and borosilicate glass forms Si-OH group that presents nucleation site of hydroxycarbonate apatite(HCA) layer. So, glassceramics of more $CaB_2O_4$ and borosilicate glass showed better bioactivity.

Nanoscale modification of magnesium with highly textural lamellar nanosheets towards increasing the corrosion resistance and bioactivity

T.S.N., S.N.,Lee, M.H. Elsevier Sequoia 2016 Surface & coatings technology Vol.304 No.-

The present study aims to modify the surface of microarc oxidation (MAO) coated Mg using an alkaline fluoride solution so as to impart a nanoscale surface feature, which would be beneficial to improve the corrosion resistance and to promote a better bioactivity. The MAO coated Mg is modified with the formation of a highly textural lamellar nanosheet-like morphology after immersion in 0.1M NaF (pH: 8.40) at 25<SUP>o</SUP>C for 120min, which completely covered the porous structure with the formation of nanosheets along with some agglomerated crystals. Thin film X-ray diffraction measurement, chemical composition analysis and Fourier transform infrared spectroscopy studies confirmed that the nanosheets are primarily Mg(OH)<SUB>2-x</SUB>F<SUB>x</SUB> while the agglomerates are NaMgF<SUB>3</SUB>. The complete coverage of the porous structure by the modified layer along with the formation of Mg(OH)<SUB>2-x</SUB>F<SUB>x</SUB> and NaMgF<SUB>3</SUB> has enabled a better corrosion resistance for MAO coated Mg modified by NaF. The higher surface area of the nanosheets favoured nucleation of monocalcium phosphate anhydrous and newberyite, both of which are biologically relevant. The unique morphological feature of the modified surface helped to achieve an improved cell adhesion and proliferation of MC3T3-E1 osteoblast-like cells. The relative growth rate of both uncoated and coated Mg are >75% on all the three days, fulfilled Grade 1 specification in terms of cytocompatibility as per ISO 10993-5 standard. The formation of nanoscale surface feature, improvement in corrosion resistance, better bioactivity and acceptable cytocompatibility point out that this methodology could be of immense help to modify the surface of Mg based absorbable implants.

Effect of fermentation on antioxidant, antimicrobial, anti-inflammatory, and anti-Helicobacter pylori adhesion activity of Ulmus davidiana var. japonica root bark

이성현,임정묵,이세원,장태후,박정희,서영석,이정호,Kamala-Kannan Seralathan,오병택 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.9

The limited yield of Ulmus davidiana var. japonica root bark (URB) extract is considered an economic loss to the food industry. Improving extraction yield and bioactivity through fermentation increase the industrial usage of URB. The study aims to optimize the fermentation with cellulolytic and pectinolytic bacteria and evaluate the bioactivity and anti-Helicobacter pylori activity of the fermented URB extract. URB fermentation with the Bacillus licheniformis FLa3, isolated from salted seafood (Sardinella zunasi), under optimal conditions (37 °C, pH 6, 10% inoculum dose, and 36 h) improved the extraction yield by 36% compared to the control. The antioxidant and antimicrobial activity of the fermented extract were significantly higher than non-fermented extract. High-performance liquid chromatography results confirmed that the fermentation increased the proportion of bioactive components such as catechin (171.7%), epicatechin (144.3%), quercetin (27.3%), and kaempferol (16.7%). The results confirmed that the fermentation increased both the extraction yield and bioactivity.

Bioactive peptides-derived from marine by-products: development, health benefits and potential application in biomedicine

Idham Sumarto Pratama,Yanuariska Putra,Ratih Pangestuti,Se-Kwon Kim,Evi Amelia Siahaan 한국수산과학회 2022 Fisheries and Aquatic Sciences Vol.25 No.7

Increased fisheries products have raised by-products that are discarded due to low economic value. In addition, marine by-products are still rich in protein and nutritional value that have biological activities and give benefits to human health. Meanwhile, there is raised pressure for sustainability practices in marine industries to reduce waste and minimize the detrimental effect on the environment. Thus, valorization by-products through bioactive peptide mining are crucial. This review focus on various ways to obtain bioactive peptides from marine by-products through protein hydrolysis, for instance chemical hydrolysis (acid and based), biochemical hydrolysis (autolysis and enzymatic hydrolysis), microbial fermentation, and subcritical water hydrolysis. Nevertheless, these processes have benefits and drawbacks which need to be considered. This review also addresses various biological activities that are favorable in pharmaceutical industries, including antioxidant, antihypertensive, anticancer, anti-obesity, and other beneficial bioactivities. In addition, some potential marine resources of Indonesia for the marine biopeptide from their by-product or undesired marine commodities would be addressed as well.