가미지패산(加味芷貝散)의 포도상구균 감염 유방염에 대한 항균활성 및 항염 효과

권지명 ( Ji Myung Kwon ),김동철 ( Dong Chul Kim ) 대한한방부인과학회 2013 大韓韓方婦人科學會誌 Vol.26 No.1

Objectives: The object of this study was to observe the protective effect of Gamijipaesan aqueous extracts (GJS), which has been traditionally used in Korean medicine in obstetrics & gynecological fields as anti-infectious and anti-inflammatory agents, against mastitis induced by Staphylococcus aureus infection in a rat model through antibacterial, antiinflammatory, immunomodulatory, and anti-oxidant effects. Methods: Antibacterial activities of GJS against S. aureus were detected using standard agar microdilution methods, with the effects on the bacterial invasion and intracellular killing of individual test materials in human mammary gland carcinoma cell(MCF-7) and murine macrophages(Raw 264.7) at MIC1/2, MIC and MIC2 concentration levels. In addition, the effects on the cell viability, nitric oxide (NO), tumor necrosis factor (TNF)-α and interleukin (IL)-6 productions of LPS activated Raw 264.7 cells. The changes on the mammary tissue viable bacterial numbers, myeloperoxidae (MPO), inducible nitric oxide synthetase(iNOS), TNF-α and IL-6 contents were observed in the S. aureus in vivo intramammary infectious rat model. The anti-bacterial and anti-inflammatory effects were compared with ciprofloxacin and piroxicam, respectively in the present study. Results: MIC of GJS and ciprofloxacin against S. aureus were detected as 0.860±0.428 (0.391-1.563) mg/ml and 0.371±0.262 (0.098-0.782) μg/ml, respectively. In addition, GJS and ciprofloxacin were also showed marked dosage-dependent inhibition of the both bacterial invasion and intracellular killing assays using MCF-7 and Raw 264.7 cells at MIC1/2, MIC and MIC×2 concentrations, respectively. ED50 against LPS-induced cell viabilities and NO, TNF-α and IL-6 releases of GJS were detected as 0.72, 0.04, 0.08 and 0.11 mg/ml, and as 19.04, 4.18, 5.37 and 4.27 μg/ml in piroxicam, respectively. 250 and 500 mg/kg of GJS also inhibit the intramammary bacterial growth, MPO, iNOS, TNF-α and IL-6 contents in S. aureus in vivo intramammary infected rats, respectively. GJS 500 mg/kg showed quite similar antibacterial and anti-infectious effects as compared with ciprofloxacin 40 mg/kg and also showed similar anti-inflammatory effects as piroxicam 10 mg/kg, in S. aureus in vivo intramammary infectious models. Conclusions: The results obtained in this study suggest that over 250 mg/kg of GJS showed favorable anti-infectious effects against S. aureus infection in a rat model through their antibacterial, anti-inflammatory, immunomodulatory and anti-oxidant effects and therefore expected that GJS can be used as alternative therapies, having both anti-inflammatory and anti-infectious activities. However, more detail mechanism studies should be conducted in future with the efficacy tests of individual herbal composition of GJS and the screening of the biological active compounds in individual herbs. In the present study, GJS 500 mg/kg showed quite similar anti-infectious effects were detected as compared with ciprofloxacin 40 mg/kg treated rats, and also GJS shows quite similar anti-inflammatory effects as compared with piroxicam 10 mg/kg in S. aureus in vivo intramammary infectious rats, but ciprofloxacin did not showed any anti-inflammatory effects, and piroxicam did not showed anti-infectious effects in this study.

봉독요법(蜂毒療法)의 근골격계질환(筋骨格界疾患) 치료기전(治療機轉)에 대한 문헌적(文獻的) 고찰(考察)

김성수,정원석,Kim, Sung-Soo,Chung, Won-Suk 척추신경추나의학회 2002 대한추나의학회지 Vol.3 No.1

Objectives : There have been many studies of the effect of Bee Venom therapy about arthritis, but no one study was reported about its whole functional mechanism to musculo-skeletal system. This study was designed to investigate the effect, Indication, and side effect of Bee Venom therapy on musculo-skeletal disease by literature review of articles. Results : The effects of Bee Venom therapy to musculo-skeletal system are divided to Anti_inflammatory effect and Anti-nociceptive effect. Anti_inflammatory effect is achieved through competitive chemotaxis, immuno-regulation, increasing of cortisol secretion by stimulating hypothalamus-pituitary gland-adrenal cortex axis. Anti-nociceptive effect is achieved by Anti-inflammatory mechanism and it works similar to anti-nociceptive effect of the acupuncture acting on central and peripheral nociceptive transduction system. The Bee Venom therapy could cause severe side effect, for example, hypersensitivity and anaphylaxis, injury to central nerve system and cardiovascular system, peripheral blood system, and renal dysfunction. Conclusions : With its Anti-inflammatory and Anti-nociceptive mechanism, Bee Venom therapy is considered that has good effects to autoimmune disease, chronic inflammation of various musculo-skeletal disease and various pain syndrome. But the clinician must be careful for its side effects.

메꽃(Calystegia pubescens Lindl.) 추출물의 항균, 항산화 및항염증 효과에 관한 연구

변지아,신운교,장예진,황수빈,이선아,김가연,이진태,송일대,권용진 한국응용과학기술학회 2024 한국응용과학기술학회지 Vol.41 No.3

Calystegia pubescens Lindl. (C. pubesens)는 전통적으로 이뇨, 피로 및 혈당 강하 효과로사용되어 온 한국 토종 식물이다. C. pubesens는 이전 연구에서 항산화 및 미백 효과를 보였지만 항균및 항염증 특성에 대한 연구가 제한적이고 기능성 소재로서의 입증이 부족하다. 본 연구에서는 70% 에탄올로 추출한 C. pubesens의 잎(CPL)과 줄기(CPS) 추출물의 항균, 항산화 및 항염증 효과를 조사했다. CPL은 CPS보다 여드름균, 황색포도상구균, 대장균과 녹농균에 대해 우수한 항균 효과를 보였다. 또한CPL은 CPS보다 DPPH 및 ABTS+ 라디칼 소거 분석에서 더 높은 항산화 활성을 나타냈다. 따라서 CPL 은 CPS에 비해 우수한 항균 및 항산화 효과를 보였다. 이러한 결과를 바탕으로 RAW264.7 대식세포를이용한 CPL의 항염증 효과를 확인하기 위한 추가 연구를 진행하였다. 지질다당류(LPS) 자극에 의해 생성된 산화질소(NO)는 CPL에 의해 감소되었다. 또한, LPS에 의해 증가된 iNOS의 단백질 및 mRNA 발현은 CPL에 의해 감소되었으며, 이는 NO 생산과 동일한 경향을 나타내었다. 결론적으로 본 연구는CPL이 CPS에 비해 우수한 항균 및 항산화 효과를 가지고 있음을 확인하였으며, LPS에 의해 유도된iNOS 발현 및 NO 생성 억제를 통해 CPL의 항염증 효과를 입증했다. 본 연구 결과를 바탕으로 CPL의기능성 소재로서의 가능성을 제시한다 Calystegia pubescens Lindl. (C. pubesens) is a native Korean herb that has been traditionally used for its diuretic, fatigue, and blood sugar-lowering effects. In previous studies, C. pubesens has shown antioxidant and whitening effects, but research on its antibacterial and anti-inflammatory properties is limited, and its potential as functional materials is lacking. In this study, we investigated the antibacterial, antioxidant, and anti-inflammatory effects of leaf (CPL) and stem (CPS) extracts of C. pubesens extracted with 70% ethanol. When the anti-bacterial effect was confirmed, CPL showed superior anti-bacterial effect than CPS for C. acne, S. aureus, E. coli and P. aeruginosa. Additionally, CPL exhibited significantly higher antioxidant activity compared to CPS, as demonstrated by DPPH and ABTS+ radical scavenging assays. Therefore, CPL showed superior anti-bacterial and antioxidant effects compared to CPS. Based on these results, further investigation was conducted to confirm the anti-inflammatory effect of CPL using RAW264.7 macrophages. Nitric oxide (NO) produced by lipopolysaccharide (LPS) stimulation was significantly reduced by CPL treatment in a dose-dependent manner. Additionally, the protein and mRNA expression of iNOS was reduced by CPL in LPS-indueced RAW264.7 cells, which was consistent with NO production. In conclusion, this study confirmed that CPL has superior anti-bacterial and antioxidant effects compared to CPS, and demonstrated anti-inflammatory effect of CPL via inhibiting iNOS expression and NO production induced by LPS. Based on the result, we suggest the potential of CPL as a valuable functional materials.

차가버섯 물 추출물의 추출온도에 따른 효능 비교 연구(II) -항산화 효능, 소염 및 항암 효과 연구-

박규천,한효상,이영종,Park, Kyu-Cheon,Han, Hyo-Sang,Lee, Young-Jong 대한본초학회 2007 大韓本草學會誌 Vol.22 No.4

Objectives : The present study purposed to compare the antioxidant effect, anti inflammatory effect and cancer cell multiplication inhibition effect of Fructificatio Inonoti Obliqui aqueous extract according to extraction temperature. Methods : We medicated animal models, which had experimental oxidation, with Fructificatio Inonoti Obliqui total extract and $50^{\circ}C$ low temperature leachate, and performed hematological analysis and blood chemical analysis with measuring SOD, GSH, catalase, NO and MDA content in the liver. In addition, we made comparative observation of anti inflammatory effect and anti-cancer effect. Results : Compared to the control group, both the group medicated with Fructificatio Inonoti Obliqui total extract and with $50^{\circ}C$ low-temperature leachate were found to decrease the number of thrombocytes in blood plasma and NO content while to increase SOD activity and catalase activity significantly. Both groups also showed anti-inflammatory effect against THP-1 cells and a multiplication inhibition effect against liver cancer cells and stomach cancer cells significantly. Conclusions : Both Fructificatio Inonoti Obliqui total extract and Fructificatio Inonoti Obliqui $50^{\circ}C$ low-temperature leachate have significant antioxidant effect, anti inflammatory effect and anti cancer effect.

Anti-Oral Microbial Activity and Anti-Inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Stimulated MC3T3-E1 Osteoblastic Cells on a Titanium Surface

( Moon-jin Jeong ),( Do-seon Lim ),( Kyungwon Heo ),( Soon-jeong Jeong ) 한국치위생과학회 2020 치위생과학회지 Vol.20 No.4

Background: The purpose of this study was to investigate the anti-oral microbial activity and anti-inflammatory effects of rosmarinic acid (RA) in lipopolysaccharide (LPS)-stimulated MC3T3-E1 osteoblastic cells on a titanium (Ti) surface during osseointegration, and to confirm the possibility of using RA as a safe natural substance for the control of peri-implantitis (PI) in Ti-based dental implants. Methods: A disk diffusion test was conducted to confirm the antimicrobial activity of RA against oral microorganisms. In order to confirm the anti-inflammatory effects of RA, inflammatory conditions were induced with 100 ng/ml of LPS in MC3T3-E1 osteoblastic cells on the Ti surface treated with or without 14μg/ml of RA. The production of nitric oxide (NO) and prostaglandin E₂ (PGE₂) in LPS-stimulated MC3T3-E1 osteoblastic cells on the Ti surface was confirmed using an NO assay kit and PGE₂ enzyme-linked immunosorbent assay kit. Reverse transcription polymerase chain reaction and western blot analysis were performed to confirm the expression of interleukin (IL)-1β and tumor necrosis factor (TNF)-α in total RNA and protein. Results: RA showed weak antimicrobial effects against Streptococcus mutans and Escherichia coli, but no antimicrobial activity against the bacteria Aggregatibacter actinomycetemcomitans and the fungus Candida albicans. RA reduced the production of pro-inflammatory mediators, NO and PGE₂, and proinflammatory cytokines, TNF-α and IL-1β, in LPS-stimulated MC3T3-E1 osteoblastic cells on the Ti surface at the protein and mRNA levels. Conclusion: RA not only has anti-oral microbial activity, but also anti-inflammatory effects in LPS-stimulated MC3T3-E1 osteoblasts on the Ti surface, therefore, it can be used as a safe functional substance derived from plants for the prevention and control of PI for successful Ti-based implants.

Anti-inflammatory Effects of Co-treatment of Jingyoganghwaltang and Cheongsimhwan on Croton Oil Induced Hemorrhoid Model in Rats

( Nayoung Jo ),( Jong-cheng Mou ),( Kanghyun Leem ),( Taeyeon Kim ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.6

Objectives : Hemorrhoids are one of the most common diseases in humans. Jingyoganghwaltang (JG) and Cheongsimhwan (CS) have been used for treating hemorrhoids in Korean traditional clinical practice. The present study was designed to evaluate the traditional effects of JG and CS on the experimental hemorrhoid model in rats. Methods : Hemorrhoids are closely related to inflammation. Accordingly, we examined the nitric oxide (NO) production in macrophage cell line in order to evaluate the anti-inflammatory effect. The expression levels of inflammation related genes including IL-1 beta, IL-6, INOS, and TNF-alpha were examined via a real-time quantitative PCR. Croton oil-induced hemorrhagic animal model was used to test the in vivo efficacy against hemorrhoids. The rectal tissues were weighed and the inflammatory proteins were measured to confirm the anti-inflammatory effects. Results : JG and CS have a statistically significant effect on inhibition of NO production and on the reduction of inflammatory gene expression such as IL-1 beta, IL-6, INOS, and TNF-alpha. The synergistic effects of co-treatment of JG and CS were found out in the IL-6 gene expression. The in vivo study using croton oil-induced hemorrhoid model in rat was performed to check the co-treatment effects. As a result, the co-treatment reduced the inflammation of the rectal tissue and decrease the inflammation related protein productions including ICAM1, MMP2 and MMP9. Conclusions : These results suggest that JG and CS co-treatment demonstrated anti-inflammatory effects in croton oil-induced hemorrhoid model in rat.

CS 약침의 항염증 및 항산화 활성에 대한 연구

김학주 ( Hak Ju Kim ),김수경 ( Soo Kyung Kim ),최일환 ( Il Hwan Choi ),정철 ( Chul Jung ),여서원 ( Hsu Yuan Lu ),두경희 ( Kyeong Hee Doo ),김민경 ( Min Kyung Kim ),조승연 ( Seung Yeon Cho ),박정미 ( Jung Mi Park ),고창남 ( Chang 대한면역약침학회 2012 대한면역약침학회지 Vol.1 No.1

Objectives: CS has been used as effective pain reducing pharmacoacupunture in clinical fields, however its constituents and physiological activity have not been yet certified by experiment. This experimental research was to analyze the component of CS Yakchim and prove its anti-oxidant and anti-inflammatory effect. Methods: Content of protein, glucose, crude fat and crude ash was calculated. Anti-oxidant effect was measured using 1,1-diphenyl-2-picryl hydrazyl(DPPH) free radical scavenging activity. Anti-inflammatory effect was measured with COX-2 kit and RAW 264.7 macrophage. the concentration of TNF-α and IL-6 was measured by Enzyme-linked immuno-sorbent assay. Results: CS Yakchim is consist of 0.17% of protein, 0.32% of glucose, 95.19% of crude fat and 0.32% of crude ash. CS showed better anti-oxidant effect than its control sample. CS COX-2 inhibiting capacity was better than its Indomethacin control sample. Inflammatory cytokine production was reduced but not at transcription level. Conclusions: These results showed that CS Yakchim is consist protein, glucose, crude fat and crude ash. CS Yakchim anti-oxidant and anti-inflammatory effect.

HO 약침액의 항염증 및 항산화 활성에 대한 연구

김학주 ( Hak Ju Kim ),최일환 ( Il Hwan Choi ),정철 ( Chul Jung ) 대한면역약침학회 2012 대한면역약침학회지 Vol.1 No.1

Objectives: The aim of this experimental study was to investigate the component of HO Yakchim and prove its anti-oxidant and anti-inflammatory effect. Methods: Content of protein, glucose, crude fat and crude ash was calculated. Anti-oxidant effect was measured using DPPH free radical scavenging activity. Anti-inflammatory effect was measured with COX-2 kit and RAW 264.7 macrophage. the concentration of TNF-α and IL-6 was measured by Enzyme-linked immuno-sorbent assay. Results: HO Yakchim is consist of 0.09% of protein, 0.23% of glucose, 97.04% of crude fat and 0.37% of crude ash. HO Yakchim showed better anti-oxidant effect than its BHA control sample. The COX-2 inhibiting capacity of HO Yakchim was better than its Indomethacin control sample. Inflammatory cytokine production was reduced but not at transcription level. Conclusions: These results represents that HO Yakchim is composed of protein, glucose, crude fat and crude ash. HO Yakchim anti-oxidant and anti-inflammatory effect.

신서란(Phormium tenax) 잎 조추출물 및 용매 분획물의 항염증 및 항아토피 효과

양권민,송상목,이도승,윤원종,김찬식,김창숙 한국자원식물학회 2019 한국자원식물학회지 Vol.32 No.5

This study describes a preliminary evaluation of the anti-inflammatory activity and anti-atopic activity ofPhormium tenax leaf extracts. P. tenax leaf was extracted using 70% ethanol and then fractionated sequentially withn-hexane, methylene chloride, ethyl acetate, n-butanol. In order to effectively screen for anti-inflammatory agents, we firstinvestigated the inhibitory effects of P. tenax leaf crude extracts and solvent fractions on production of pro-inflammatoryfactors[nitric oxide(NO), prostaglandin E2(PGE2), inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2)]and pro-inflammatory cytokines [tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-1β(IL-1β)] inlipopolysaccharide(LPS)-stimulated RAW 264.7 cells. In addition, we also evaluated of their inhibitory effect on the atopicdermatitis-like inflammatory markers such as macrophage-derived chemokine(MDC) and thymus and activation-regulatedchemokine(TARC) in HaCaT cells. Among the five solvent fractions of P. tenax, methylene chloride and ethyl acetatefractions inhibited production of pro-inflammatory factors and pro-inflammatory cytokines in a dose dependent manner,respectively. These fractions were also showed inhibitory activity for MDC and TARC expression levels in IFN-γ-stimulated HaCaT cells, respectively. These results suggest that P. tenax have significantly effects of anti-inflammatoryactivity and anti-atopic activity that might be beneficial for the topical treatment of inflammatory skin disorders. 본 연구는 하라케케(Harakeke)로 불리는 신서란(Phormiumtenax)를 화장품 및 의약품산업의 기능성 소재로서의 이용 가능성을 확인하기 위하여 신서란 잎을 대상으로 70% 에탄올 추출물과 용매 분획물을 제조하여, 이것들의 항염증 및 항아토피의효과를 조사하였다. LPS로 유도된 RAW 264.7 세포에서 신서란에탄올 추출물과 용매 분획물의 항염증 효과를 조사한 결과,methylene chloride와 ethyl acetate 분획물에서 NO와 PGE2 생성 억제 활성이 가장 높게 나타났으며, 농도 의존적으로 NO와 PGE2 생성 억제 활성을 보였다. 또한, 이들 분획물에서는iNOS 및 COX-2 발현 억제 활성을 보였다. 신서란 잎 조추출물과 용매 분획물에 의한 NO, PGE2 생성 억제 활성이 NOS 및COX-2 발현 억제에 의한 것임을 제시한다. 더불어, hIFN-γ로자극된 HaCaT 세포에 용매 분획물을 처리하여 MDC 및 TRAC생성억제 효과를 조사한 바, methylene chloride 분획물은MDC 및 TATC의 생성을 각각 65%, 52% 생성억제 시켰으며,ethyl acetate 분획물은 MDC 및 TATC의 생성을 각각93%, 84%억제 효과를 보였다. 이상의 결과는 신서란 잎 조추출물과 용매분획물을 이용한 항염증 및 항아토피 효능을 갖는 유효성분 분리 및 활용화 연구에 중요한 기초자료가 될 것이며, 기능성 화장품, 의약외품 및 의약품 소재 개발에 적용 가능성이 높다고 사료된다.

蜂毒療法의 筋骨格界疾患 治療機轉에 대한 文獻的 考察

정원석(Won-Suk Chung),김성수(Sung-Soo Kim) 척추신경추나의학회 2002 대한추나의학회지 Vol.3 No.1

Objectives : There have been many studies of the effect of Bee Venom therapy about arthritis, but no one study was reported about its whole functional mechanism to musculo-skeletal system. This study was designed to investigate the effect, indication, and side effect of Bee Venom therapy on musculo-skeletal disease by literature riview of articles. Results : The effects of Bee Venom therapy to musculo-skeletal system are divided to Anti-inflammatory effect and Anti-nociceptive effect. Anti-inflammatory effect is achieved through competitive chemotaxis, immuno-regulation, increasing of cortisol secretion by stimulating hypothalamus-pituitary gland-adrenal cortex axis. Anti-nociceptive effect is achieved by Anti-inflammatory mechanism and it works similar to anti-nociceptive effect of the acupuncture acting on central and peripheral nociceptive transduction system. The Bee Venom therapy could cause severe side effect, for example, hypersensitivity and anaphylaxis, injury to central nerve system and cardiovascular system, peripheral blood system, and renal dysfunction. Conclusions : With its Anti-inflammatory and Anti-nociceptive mechanism, Bee Venom therapy is considered that has good effects to autoimmune disease, chronic inflammation of various musculo-skeletal disease and various pain syndrome. But the clinician must be careful for its side effects.