Rechargeable microbial anti-adhesive polymethyl methacrylate incorporating silver sulfadiazine-loaded mesoporous silica nanocarriers

Jo, J.K.,El-Fiqi, A.,Lee, J.H.,Kim, D.A.,Kim, H.W.,Lee, H.H. Munksgaard ; Elsevier Science Ltd 2017 Dental materials Vol.33 No.10

Objectives: Even though polymethyl methacrylate (PMMA) resin is widely used as a dental material, it has poor microbial anti-adhesive properties, which accelerates oral infections. In this investigation, silver-sulfadiazine (AgSD)-loaded mesoporous silica nanoparticles (Ag-MSNs) were incorporated into PMMA to introduce long-term microbial anti-adhesive effects and to make PMMA a rechargeable resin. Methods: After characterization of the Ag-MSNs in terms of their mesoporous characteristics and drug loading capacity, the 3 point flexural test and hardness were evaluated in PMMA incorporating Ag-MSNs (0.5, 1, 2.5 and 5%). Anti-adhesive effects were observed for Candida albicans and Streptococcus oralis with experimental specimens for up to 28days and after recharging with AgSD. Results: A typical spherical morphology and high mesoporosity were observed for the MSNs used for loading AgSD. Incorporation of Ag-MSNs into PMMA (0.5, 1, 2.5 and 5%) sustained its flexural strength but increased its surface hardness. Anti-adhesive effects were observed after 1h of exposure to both microbial species, and the effects accelerated with increasing Ag-MSN incorporation into PMMA. Long-term microbial anti-adhesive effects were observed for up to 14 days, and further long-term (7 days) anti-adhesive effects were observed after reloading the Ag-MSN-incorporated PMMA (aged for 28 days) with AgSD; these effects were largely caused by released silver ions and partially by changes in surface hydrophilicity. No cytotoxicity to keratinocytes was observed. Conclusions: The improved mechanical properties and the prolonged microbial anti-adhesive effects, which lasted after reloading of the drug, suggest the potential usefulness of Ag-MSN-incorporated PMMA as a microbial anti-adhesive dental material. Significance: Ag-MSN-incorporated PMMA can be used as a microbial anti-adhesive dental material for dentures, orthodontic devices and provisional restorative materials.

Oleamide–PDMS copolymer for coating urinary catheters with anti-adhesive and anti-biofilm properties

Park Jiwon,Seo Eunseok,김양훈,안지영,최다현,이경은,박윤신 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.3

Background Biofilm formation on biomedical devices is a prevalent problem that can result in several complications and is responsible for over 80% of all clinical infections. Successful treatment of biofilms requires a 1500-fold increase in antibiotic concentration, which can lead to toxicity and antibiotic resistance. Therefore, biofilm growth and infection in biomedical devices are significant concerns, and their prevention is a crucial medical challenge. Objectives To prevent biofilm infection by modifying the surface properties of medical devices, an oleamide–polydimethylsiloxane (PDMS) copolymer was synthesized to demonstrate anti-adhesion to bacteria and anti-biofilm activity. Catheter coatings for biomedical device applications were evaluated by blood toxicity. Results We synthesized an oleamide–PDMS copolymer (OPC) and evaluated its anti-adhesion and anti-biofilm activities against the gram-negative bacterium Escherichia coli (E. coli) and the gram-positive bacterium Staphylococcus aureus (S. aureus). The OPC films inhibited the growth of biofilms by inhibiting early adhesion of bacteria. As the oleamide content increased, the ability of the OPC films to inhibit E. coli and S. aureus surface adhesion also increased. In addition, the biofilm formation ability of both E. coli and S. aureus was significantly inhibited at oleamide contents of 2.5 wt% and 5 wt% in the OPC films, respectively. OPC films were applied to the catheter using a simple dip-coating method, and a low hemolytic capacity was confirmed by hemolysis analysis. Conclusion The anti-adhesion ability of OPC enables them to prevent biofilm growth and infection. Furthermore, they can be applied in medical devices owing to their high biocompatibility and poor hemolytic properties. Background Biofilm formation on biomedical devices is a prevalent problem that can result in several complications and is responsible for over 80% of all clinical infections. Successful treatment of biofilms requires a 1500-fold increase in antibiotic concentration, which can lead to toxicity and antibiotic resistance. Therefore, biofilm growth and infection in biomedical devices are significant concerns, and their prevention is a crucial medical challenge. Objectives To prevent biofilm infection by modifying the surface properties of medical devices, an oleamide–polydimethylsiloxane (PDMS) copolymer was synthesized to demonstrate anti-adhesion to bacteria and anti-biofilm activity. Catheter coatings for biomedical device applications were evaluated by blood toxicity. Results We synthesized an oleamide–PDMS copolymer (OPC) and evaluated its anti-adhesion and anti-biofilm activities against the gram-negative bacterium Escherichia coli (E. coli) and the gram-positive bacterium Staphylococcus aureus (S. aureus). The OPC films inhibited the growth of biofilms by inhibiting early adhesion of bacteria. As the oleamide content increased, the ability of the OPC films to inhibit E. coli and S. aureus surface adhesion also increased. In addition, the biofilm formation ability of both E. coli and S. aureus was significantly inhibited at oleamide contents of 2.5 wt% and 5 wt% in the OPC films, respectively. OPC films were applied to the catheter using a simple dip-coating method, and a low hemolytic capacity was confirmed by hemolysis analysis. Conclusion The anti-adhesion ability of OPC enables them to prevent biofilm growth and infection. Furthermore, they can be applied in medical devices owing to their high biocompatibility and poor hemolytic properties.

Evaluation of Anti-Sapstain Activity of Rice Powder Adhesives Modified with Wood Preservatives

Min Lee,Eun Chang Kang,Sang Min Lee 한국목재공학회 2016 목재공학 Vol.44 No.6

Demand of natural interior finishing material has been widely sprayed in nowadays because many weak people as children, pregnant women, and elder people are being struggled with sick house syndrome due to volatile organic compounds such as formaldehyde, toluene, benzene, etc. Our research group developed a no-added formaldehyde adhesive for wood-based panels from mainly rice powder and some additives in the previous study for abating sick house syndrome. Since the rice powder adhesive provides a good source of nutrients with microorganisms, it was suspected a susceptibility of the rice powder adhesive to fungal and sapstain attack. We evaluated anti-sapstain activity of the rice powder adhesives modified by adding wood preservatives. We modified the rice powder adhesive by adding three different types of anti-sapstain preservatives at three different concentrations to assess their anti-sapstin activity. The bonding strengths of the modified rice powder adhesives were still outstanding performance on all samples. Moreover, the plywood manufactured with the modified rice powder adhesive satisfied outdoor use requirement for ordinary plywood (KS F3101, Korean Standard). The results obtained showed that at least 3% of preservative should be added to the rice powder adhesive to obtain effective anti-sapstain activity.

Anti-cell Adhesive Effect of Phenylacetylshikonin Analogues Related to their Cytotoxicity in A549 Cells

Kim, Seon-Hee,Song, Gyu-Yong,Sok, Dai-Eun,Ahn, Byung-Zun The Pharmaceutical Society of Korea 1997 Archives of Pharmacal Research Vol.20 No.2

An attempt to estabilish the relationship between anti-cell adhesive action of phenylacetylshikonin analogues and their cytotoxicity against A549 cells was done. In the one hour incubation with A549 cells,${\alpha}$-methoxyphenylacetyl-(9), ${\alpha}$-acetoxyphenylacetyl-(13), 3,4-methylenedioxyphenylacetyl-(15) and 4-(N,N-dimethylamino)-phenylacetylshikonin (17) analogues showed a high anti-cell adhesive activity $(IC_100; value, 4-8{\mu}g/ml)$, while halophenylacetyl- and dimethoxy- or trimethoxyphenylacetyl analogues expressed no activity at $40{\mu}g/ml$, indicating that the presence of a bulky group at $ C^I-{\alpha}$ and a polar group at C-4 of phenylacetyl moiety may be important. A similar structure activity relationship exists for the 48 hr cytotoxocity $(ED_{50})$ of phenylacetylshikonin analogues in A 549 cells, but not in either K562 or L1210 cells. Furthermore, the difference between $IC_{100}$ values for anti-cell adhesive activity and$ED_{50}$ values for cytotoxicity of potent compound in A549 cells was not so great (1.5 to 3 times). Based on these observations, it is proposed that the anti-cell adhesive action of phenylacetylshikonins might be responsible for their cytotoxicity in A549 cells.

Anti-slip 제어기를 이용한 유도전동기 병렬운전

金重敎(Jung-Gyo Kim),李柱(Ju Lee) 대한전기학회 2006 전기학회논문지 P Vol.55 No.1

In electric motor coaches, the rolling stocks move by the adhesive effort between rail and driving wheel. Generally, the adhesive effort is defined by the function of both the weight of electric motor coach and the adhesive effort between rails and driving wheel. The characteristics of adhesive effort is strongly affected by the conditions between rails and driving wheel. When the adhesive effort decreases suddenly, the electric motor coach has slip phenomena. This paper proposes a anti-slip control algorithm which uses the maximum adhesive effort by instantaneous estimation of adhesion force using load torque disturbance observer. Based on this estimated adhesive effort, the anti-slip control is performed to obtain the maximum transfer of the tractive effort..

A Hydrogel-based First-Aid Tissue Adhesive with Effective Hemostasis and Anti-bacteria for Trauma Emergency Management

Dongjie Zhang,Li Mei,Yuanping Hao,Bingcheng Yi,Jilin Hu,Danyang Wang,Yaodong Zhao,Zhe Wang,Hailin Huang,Yongzhi Xu,Xuyang Deng,Cong Li,Xuewei Li,Qihui Zhou,Yun Lu 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Clinical tissue adhesives remain some critical drawbacks for managing emergency injuries, such as inadequate adhesive strength and insufficient anti-infection ability. Herein, a novel, self-healing, and antibacterial carboxymethyl chitosan/polyaldehyde dextran (CMCS/PD) hydrogel is designed as the first-aid tissue adhesive for effective trauma emergency management. Methods We examined the gel-forming time, porosity, self-healing, antibacterial properties, cytotoxicity, adhesive strength, and hemocompatibility. Liver hemorrhage, tail severance, and skin wound infection models of rats are constructed in vivo, respectively. Results Results demonstrate that the CMCS/PD hydrogel has the rapid gel-forming (~ 5 s), good self-healing, and effective antibacterial abilities, and could adhere to tissue firmly (adhesive strength of ~ 10 kPa and burst pressure of 327.5 mmHg) with excellent hemocompatibility and cytocompatibility. This suggests the great prospect of CMCS/ PD hydrogel in acting as a first-aid tissue adhesive for trauma emergency management. The CMCS/PD hydrogel is observed to not only achieve rapid hemostasis for curing liver hemorrhage and tail severance in comparison to commercial hemostatic gel (Surgiflo ®) but also exhibit superior anti-infection for treating acute skin trauma compared with clinical disinfectant gel (Prontosan ®). Conclusions Overall, the CMCS/PD hydrogel offers a promising candidate for first-aid tissue adhesives to manage the trauma emergency. Because of the rapid gel-forming time, it could also be applied as a liquid first-aid bandage for mini-invasive surgical treatment.

Potent anti-adhesion agent using a drug-eluting visible-light curable hyaluronic acid derivative

Han, Ga-Dug,Kim, Jae-Won,Noh, Seung-Hyun,Kim, Shin-Woong,Jang, Eui-Chan,Nah, Jae-Woon,Lee, Young-Gi,Kim, Mi-Kyung,Ito, Yoshihiro,Son, Tae-Il Elsevier 2019 Journal of industrial and engineering chemistry Vol.70 No.-

<P><B>Abstract</B></P> <P>Post-operative adhesion is a very serious complication in the clinical field, frequently occurring after surgery. In severe cases, reoperation may be required. In this study, a visible light-curable furfuryl hyaluronic acid derivative was prepared and combined with ibuprofen for anti-adhesion and anti-inflammatory applications. A furfuryl isocyanate moiety was introduced in hyaluronic acid affording furfuryl hyaluronic acid (F-HA). The anti-adhesion and anti-inflammatory effects of F-HA were examined in chickens at 2, 4, and 8 weeks postoperation. It was concluded that F-HA combined with ibuprofen is a suitable anti-adhesion and anti-inflammation agent.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Developed anti-adhesion agent that can photo-immobilization drug by using visible light alginate derivative

김신웅,이태훈,민성준,손태일 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0

This study demonstrated the anti-adhesion and wound healing effect of a visible light curable anti-adhesion agent using an alginate derivative modified with a furfuryl moiety. The cytotoxicity, cell adhesion, and cell permeability of the F-Alg were evaluated for use in anti-adhesion applications. Drug immobilization and protein release were assessed to verify whether the alginate derivatives and drugs were photo-immobilized. In in vivo anti-adhesion testing, the new anti-adhesion agent prepared in this study acted as a physical protective layer by forming a biofilm on the surgical site. Additionally, along with gradual decomposition of the photo-crosslinked alginate derivative, the immobilized drug was released, and additional effects such as accelerated wound healing are expected. Thus, visible light-curable F-Alg has good application potential as an anti-adhesion agent.

의료용 접착제 및 유착방지막의 최신 연구개발 동향

이종호,김현용,정태곤,한인호,박종철,박기동,최재봉,현승휴,한동근,한동욱 한국생체재료학회 2010 생체재료학회지 Vol.14 No.2

In the case of surgical operation for the treatment of cancers, vascular diseases and diabetes mellitus, the use of medical (or biotissue) adhesives is gradually increasing instead of suturing. Together with this trend, the development of anti-adhesion membranes (or barriers) is rapidly increasing for preventing complications originated from post-operative tissue adhesions frequently occurring. In general, all medical devices should pass the testing for biological safety and effectiveness. Thus, newly developed medical adhesives and anti-adhesion membranes must be evaluated according to the well-regulated standard guidelines, such as ISO, OECD, ASTM, etc, under authorized institution, otherwise they cannot be commercialized. In this review, medical adhesives and anti-adhesion membranes were categorized and compared according to their use and materials followed by a brief description of the properties of products commercially available. Furthermore, this review dealt with the present state of commercialization, the size of market and the recent trends of research and development regarding medical adhesives and anti-adhesion membranes

Biocompatible, drug-loaded anti-adhesion barrier using visible-light curable furfuryl gelatin derivative

Kim, Eun-Hye,Kim, Jae-Won,Han, Ga-Dug,Noh, Seung-Hyun,Choi, Jae-Hee,Choi, ChangSun,Kim, Mi-Kyung,Nah, Jae-Woon,Kim, Tae-Yeon,Ito, Yoshihiro,Son, Tae-Il Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.120 No.1

<P><B>Abstract</B></P> <P>Recently, many of studies have been attempted to determine how to decrease adhesion. To effectively prevent adhesion, decrease in unnecessary surgical procedures, prevention of contact with other tissue, and drug treatment for inflammation are required. However, current anti-adhesion materials have disadvantages. To solve current problems, we prepared a biocompatible drug-loaded anti-adhesion barrier using a visible-light curable furfuryl gelatin derivative. We used riboflavin as a photo-initiator in the photo-curing process. The biocompatibility of riboflavin was estimated compared with that of Rose Bengal. In addition, the curing ratio was measured to determine whether riboflavin initiated photo-curing. We also evaluated the curing ratio of riboflavin according to the concentration of F-gelatin and the photo-irradiation time. A drug used to decrease inflammation that causes adhesion should not disappear from the surgical site and should also be released consistently. For this, we observed the release profiles of photo-immobilized ibuprofen with different concentrations of F-gelatin. Because an anti-adhesion barrier should protect from bacterial infection we evaluated the protective ability of a barrier formed by F-gelatin. In conclusion, a drug-loaded anti-adhesion barrier was prepared using a visible-light curable furfuryl gelatin derivative, with riboflavin as a photo-initiator. We expect that this drug-loaded anti-adhesion barrier effectively decrease adhesion formation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Riboflavin showed the better photo-curable property and biocompatibility as photo-initiator than Rose Bengal. </LI> <LI> As concentration of furfuryl gelatin and photo-irradiation time increase, the photo-curing ratio increase. </LI> <LI> Release profile of photo-immobilized ibuprofen can be controlled by changing the concentration of furfuryl gelatin. </LI> <LI> Surgical site can be protected from bacterial infection by furfuryl gelatin barrier and adhesion also can be decreased. </LI> <LI> Drug-loaded anti-adhesion barrier can protect surgical site and prevent inflammation. </LI> </UL> </P>