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- 주제어 : Stimulated saliva (검색결과 9 건)
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김아현,김영남,권호근,김백일 한국식물생명공학회 ( 구 한국식물조직배양학회 ) 2008 한국식물생명공학회 춘계학술연구발표회 Vol.32 No.3
Objective: The aim of this study was to evaluate erosive potential of commercial fruit flavored candies in vitro and in vivo using candy stimulated human saliva. Methods: Three kinds of commercial fruit flavored sour candies (pomegranate, plum, lemon) evaluated in this study. According to measurement of pH in vitro, candy solutions were prepared by dissolving 4 grams of candy powder in 20 ml of distilled water or artificial saliva. For in vivo evaluation, Saliva was collected from 12 healthy non-medicated volunteers. The candies were given in a randomized order and test persons were blinded as to which candy subjects were having. Collections of whole saliva lasted 19 minutes and were divided into four periods: 5 minutes; non-stimulated saliva, 4 minutes; candy-stimulated saliva, and 10 minutes; post-stimulated saliva which was divided into two steps. For evaluating erosive potential of each candy, 5 mg of pure hydroxyapatite (HAp) powder was added to 5 ml of candy-stimulated saliva. Then the pH was monitored for 5 minute at 15 seconds intervals under constant stirring. Immediately after dissolution of HAp, a back titration with acid (1 M HCl) was performed to estimate how much HAp was dissolved. Results: The pH of candies dissolved in distilled water and artificial saliva of in vitro evaluation were < 5.5. In terms of in vivo trial, pH of the candy-stimulated saliva of all samples was lower than pH of original non-stimulated. Each candy-stimulated saliva was dissociated HAp powder. Among them plum candy-stimulated saliva showed the highest solubility. Conclusion: In this study, fruit flavored acidic candies induced an erosive environment in the oral cavity during intake and those have erosive potential. Especially plum candy showed higher erosive potential than other candies.
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혼합치열기 어린이에서 mutans streptococci에 대한 자일리톨 껌의 향균효과에 관한 연구
박재홍 大韓小兒齒科學會 2007 大韓小兒齒科學會誌 Vol.34 No.4
Xylitol 껌의 mutans streptococci(MS)에 대한 항균효과를 평가하기 위하여 6세∼11세 사이의 혼합치열기 어린이 62명을 선정하였다. 4개월 동안 1개의 xylitol 껌을 하루 3회 씹는군,2개의 xylitol 껌을 하루 3회 씹는 군과 xylitol 껌을 씹지 않는 대조군으로 나누어 하루 3번 식후 최소 5분간 껌을 씹게 한 후 1개월 간격으로 자극성타액을 채취하여 선택배지 (mitis salivarius kanamicin bacitracin agar)에 배양한 후 MS 군락 수의 감소를 평가하였다. 1회 1개의 xylitol 껌을 씹는 군에서는 MS의 감소를 보이지 않았고,1회 2개의 xylitol 껌을 씹는 군에서 4개윌째 MS의 유의한 감소를 보였다(P < 0.05). 따라서 혼합치열기 어린이의 경우 하루 3회,총 10g 정도의 xylitol을 4개월 이상 꾸준히 사용하였을 경우 치아우식증에 대한 예방효과가 있을 것으로 사료된다. The purpose of this study was to investigate the effect of xylitol chewing gums on mutans streptococci(MS) counts in saliva. Sixty two children 6 to 11 years old were randomly assigned into one of three groups, Stimulated saliva specimens were plated in duplicate on conventional selective culture (mitis salivarius kanamycin bacitracin agar) for mutans streptococci. Polymerase chain reaction (PCR) amplication was performed to identify MS. After the 4-month period a significant decrease of the MS counts occurred in the group B (two gum 3 times a day: P < 0.05) but not in group A (one gum 3 times a day) and control group (didn t receive xylitol gum). According to qualitative evaluations, xylitol use did reduce the levels of MS in mixed dentition children. It has been suggested that a daily intake of 2 tablet for 3 times a day (about lOg) is needed in order to obtain a clinical anticariogenic effect.
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Caries-related Microbiological Screening in Children under Three Years of Age
Park, Jae-Hong,Lee, Keung-Ho 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.4
3세 이하의 어린이에서 구강 내 미생물과 타액완충능이 치아우식증과의 관련성을 알아보았다. 87명의 어린이를 실험대상으로 하여 치태와 자극성타액을 각각 면봉과 면구를 이용하여 채취하였다. 0.94%의 lactic acid를 타액 표본에 첨가하기 전과 후의 pH를 각각 측정하였고 타액표본을 순차적으로 희석하여 선택배지와 비선택배지에 접종하였다. Mutans streptococci (MS), lactobacilli (LB), total viable count (TVC)의 집락수와 타액의 pH와 완충능을 어린이의 치아우식과 비교하여 다음과 같은 결과를 얻었다. 1. 자극성타액과 치태 모두에서 MS 와 LB 가 치아우식증과 높은 통계학적 유의성을 보였다. 2. 치아우식의 발생을 예측할 수 있는 미생물의 집락수는 다음과 같았다. 1) 자극성타액 1 ml 당 MS 집락수가 10^5 이상 2) 치태 1 ml 당 MS 집락수가 2×10^5 이상 3) 자극성타액 1 ml 당 LB 집락수가 10^3 이상 4) 치태 1 ml 당 LB 집락수가 10^3 이상 3. 타액의 pH와 완충능은 유아기우식증과 관련이 없었다. 4. MS 검사는 LB 검사 보다 높은 예측치 (predictive value)와 교차비 (odds ratio)를 보였다. 5. MS 집락수는 어린이에 있어서 치아우식 발생을 예측할 수 있는 가장 믿을만한 미생물학적 검사로 일반적인 세균배양법을 이용하거나 상품화된 검사기구를 이용하여 쉽게 임상에 적용할 수 있다. To evaluate microbial data and salivary measurements from clinically compatible, culture-based screening procedures employed with children younger than 36 months old. Plaque and stimulated saliva specimens were collected from 87 children. The pH of each saliva sample was measured before and after 0.94% lactic acid was added. Specimens were diluted and plated on selective media and non-selective media. Data collected were counts of mutans streptococci (MS) and lactobacilli (LB). In addition, total viable counts (TVC) of specimens, salivary pH and buffering capacity were also assessed. Each variable was compared to caries status of subjects. According to this study, the results were as followed: 1. Highly significant correlation with caries rates were found for counts of MS and LB. 2. The specific counts/ml saliva of plaque above which caries is predicted, or below which caries is not predicted were as follows: 1) Saliva MS; 10^5 2) Plaque MS; 2×10^5 3) Saliva LB; 10^3 4) Plaque LB; 10^3 3. Salivary pH and buffering capacity versus caries status were significant. 4. Microbial screening methods based on mutans streptococci had higher predictive values and odds ratios than methods for lactobacilli. 5. MS counts were clearly the best indicators of caries status in young children. This measurement can easily be obtained in a dental clinical setting both by conventional culture techniques, or commercial kits for MS recovery.
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혼합치열기 어린이에서 Streptococcus mutans와 Streptococcus sobrinus의 분포도 조사
이명성,최성철,박재홍 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.2
본 연구는 125명의 6-11세 혼합치열기 아동을 대상으로 연령에 따라 초기 혼합치열기 A군(6-8세; 62명)과 후기 혼합치열기 B군(9-11세; 63명)으로 분류하고 유치의 dfs, 영구치의 DFS를 기록한 후 자극성 타액을 채취하여 TYCSB 배지에 배양해서 S. mutans와 S. sobrinus를 구분하였으며 PCR을 시행하여 확인하였다. S. mutans와 S. sobrinus의 분포도와 치아우식증과의 상관관계를 조사하여 다음과 같은 결과를 얻었다. 1. S. mutans와 S. sobrinus의 상관계수는 A군 0.70, B군 0.50로 두 군 모두에서 높은 상관관계를 보였다. 2. S. mutans와 치아우식증과의 관계는 A군(r=0.25)과 B군(r=0.34) 모두에서 약간의 관련성이 나타났다. 3. A군에서 S. sobrinus와 치아우식증간에 상관관계가 없는 것으로 나타났으며, B군(r=0.21)에서는 미약한 상관관계가 성립되었다. 4. S. mutans와 나이의 상관관계는 A군과 B군 모두에서 관련성이 없는 것으로 나타났다. 5. S. sobrinus와 나이의 상관관계는 A군(r=0.32)에서는 약간의 관련성이 나타났고, B군에서는 관련성이 없는 것으로 나타났다. Mutans streptococci have been reported to be implicated in dental caries. Of these streptococcal species, Streptcoccus mutans and Streptococcus sobrinus are most commonly found in human dental caries. Prevalence of these bacterial species in dental caries is found to be varied in different races and countries. Therefore, importance of these bacteria in dental caries remains to be determined. The present study was performed to find out correlation S. mutans and S. sobrinus with dental caries in 125 Korean children with mixed dentition between 6 to 11 years of age. They were classified as group A(6-8 years) and group B(9-11 years) by age. For the study, stimulated saliva samples were collected from each subject. The vials containing saliva specimens were serially diluted (1:10) in saline and plated in duplicate on tryptone-yeast extract-cysteine with sucrose and bacitracin (TYCSB) for S. mutans and S. sobrinus. After genomic DNA was extracted from the samples, polymerase chain reaction (PCR) amplification was performed for identification using universal primers and specific primers to S. mutans and S. sobrinus. Data of microbial variables were compared to caries status of the subjects. According to this study, the result were as follows: 1. S. mutans versus S. sobrinus were moderate positive linear correlated in both group A(r=0.70) and group B(r=0.50). 2. Between S. mutans and dental caries, there were weak positive linear correlation in both group A(r=0.25) and group B(r=0.34). 3. S. sobrinus versus dental caries were not correlated in group A but slightly correlated in group B(r=0.21). 4. Between S. mutans and age, there were not correlation in both group. 5. S. sobrinus versus age were weak correlated in group A(r=0.32) but not correlated in group B.
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