악성림프종에서 SV40 Large T Antigen의 검색

김영아,백진호,김철우,윤선옥,전윤경,장미수,김지은 대한병리학회 2009 Journal of Pathology and Translational Medicine Vol.43 No.4

Background : The association of simian virus 40 (SV40) with certain types of human cancers, including malignant lymphomas, has been a topic of interest for some time. Although the virus is distributed worldwide, its incidences vary according to the specific types of tumors, and the epidemiological areas. The aim of this study was to investigate the frequency of SV40 in malignant lymphomas among Korean patients. Methods : One hundred seventy three cases of malignant lymphomas were evaluated by immunohistochemical staining for SV40 large T antigen (TAg), using an extremely sensitive, tyramide based, catalyzed signal amplification method. Results : From 158 non-Hodgkin’s lymphomas, including 115 diffuse large B-cell lymphomas, and 15 Hodgkin’s lymphomas, none of the cases were positive for SV40 TAg. Conclusions : SV40 does not appear to be related to the pathogenesis of malignant lymphomas among Koreans. Background : The association of simian virus 40 (SV40) with certain types of human cancers, including malignant lymphomas, has been a topic of interest for some time. Although the virus is distributed worldwide, its incidences vary according to the specific types of tumors, and the epidemiological areas. The aim of this study was to investigate the frequency of SV40 in malignant lymphomas among Korean patients. Methods : One hundred seventy three cases of malignant lymphomas were evaluated by immunohistochemical staining for SV40 large T antigen (TAg), using an extremely sensitive, tyramide based, catalyzed signal amplification method. Results : From 158 non-Hodgkin’s lymphomas, including 115 diffuse large B-cell lymphomas, and 15 Hodgkin’s lymphomas, none of the cases were positive for SV40 TAg. Conclusions : SV40 does not appear to be related to the pathogenesis of malignant lymphomas among Koreans.

No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea

Eom, Minseob,Abdul-Ghafar, Jamshid,Park, Sun-Mi,Han, Joung Ho,Hong, Soon Won,Kwon, Kun Young,Ko, Eun Suk,Kim, Lucia,Kim, Wan Seop,Ha, Seung Yeon,Lee, Kyo Young,Lee, Chang Hun,Yoon, Hye Kyoung,Choi, Yo The Korean Society of Pathologists and The Korean 2013 KOREAN JOURNAL OF PATHOLOGY - Vol.47 No.2

<P><B>Background</B></P><P>Simian virus 40 (SV40), a polyomavirus, was discovered as a contaminant of a human polio vaccine in the 1960s. It is known that malignant mesothelioma (MM) is associated with SV40, and that the virus works as a cofactor to the carcinogenetic effects of asbestos. However, the reports about the correlation between SV40 and MM have not been consistent. The purpose of this study is to identify SV40 in MM tissue in Korea through detection of SV40 protein and DNA.</P><P><B>Methods</B></P><P>We analyzed 62 cases of available paraffin-blocks enrolled through the Korean Malignant Mesothelioma Surveillance System and performed immunohistochemistry for SV40 protein and real-time polymerase chain reaction (PCR) for SV40 DNA.</P><P><B>Results</B></P><P>Of 62 total cases, 40 had disease involving the pleura (64.5%), and 29 (46.8%) were found to be of the epithelioid subtype. Immunostaining demonstrated that all examined tissues were negative for SV40 protein. Sufficient DNA was extracted for real-time PCR analysis from 36 cases. Quantitative PCR of these samples showed no increase in SV40 transcript compared to the negative controls.</P><P><B>Conclusions</B></P><P>SV40 is not associated with the development of MM in Korea.</P>

No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea

엄민섭,정순희,Jamshid Abdul-Ghafar,박선미,한정호,홍순원,권건영,고은숙,김루시아,김완섭,하승연,이교영,이창훈,윤혜경,최유덕,정명자 대한병리학회 2013 Journal of Pathology and Translational Medicine Vol.47 No.2

Background: Simian virus 40 (SV40), a polyomavirus, was discovered as a contaminant of a human polio vaccine in the 1960s. It is known that malignant mesothelioma (MM) is associated with SV40, and that the virus works as a cofactor to the carcinogenetic effects of asbestos. However, the reports about the correlation between SV40 and MM have not been consistent. The purpose of this study is to identify SV40 in MM tissue in Korea through detection of SV40 protein and DNA. Methods: We analyzed 62 cases of available paraffin-blocks enrolled through the Korean Malignant Mesothelioma Surveillance System and performed immunohistochemistry for SV40 protein and real-time polymerase chain reaction (PCR) for SV40 DNA. Results: Of 62 total cases, 40 had disease involving the pleura (64.5%), and 29 (46.8%) were found to be of the epithelioid subtype. Immunostaining demonstrated that all examined tissues were negative for SV40 protein. Sufficient DNA was extracted for real-time PCR analysis from 36 cases. Quantitative PCR of these samples showed no increase in SV40 transcript compared to the negative controls. Conclusions: SV40 is not associated with the development of MM in Korea.

Nonkaryophilic T Antigen of Simian Virus 40 does not have the Functions of Transcriptional Regulation and Transformation

Kim, Gwi-Sook,Kim, Yi-Hwa,Kim, Dong-Wan The Korean Society for Microbiology 2001 Journal of Bacteriology and Virology Vol.31 No.2

The T antigen of simian virus 40 (SV40) is a multifunctional phosphoprotein normally locating in the cell nucleus. It is implicated in the regulation of viral and cellular gene expression, in the replication of DNA and in the transformation of infected cells. Here, we have constructed the expression system of karyophilic and nonkaryophilic T antigens, and investigated their activities of transcriptional regulation and transformation in the African green monkey kidney CV1 cells and the rat fibroblast 3Y1 cells. The wild type, karyophilic T antigen showed the transcriptional regulatory activities of trans-activation and trans-suppression on the SV 40 promoter and also showed the transforming activity. But the mutant, nonkaryophilic T antigen showed none of the activities of transcriptional regulation and transformation. These results indicate that the nuclear localization of the SV40 T antigen is necessary for the regulation of gene expression and transformation of infected cells. Our results also suggest that the function of transcriptional regulation might be closely related to the transformation of cells by T antigen.

Analysis of the Stoichiometry and the Domain for Interaction of Simian Virus 40 Small-t Antigen with Protein Phosphatase 2A

Yang, Sung-Il,Mumby, Marc C. Korean Society for Biochemistry and Molecular Biol 1995 Journal of biochemistry and molecular biology Vol.28 No.4

Simian virus 40 (SV40) small-t antigen (small-t) has been known to regulate the activity of a cellular enzyme, protein phosphatase 2A (PP2A), composed of A. B, and C subunits, via binding to the A subunit In the study presented here, the stoichiometry of the binding of small-t to PP2A was determined to be 1: 1. It was also shown that small-t binds to the AC form of PP2A with a higher apparent affinity than it binds to the free A subunit. We also characterized the interaction of PP2A with wild-type and various mutant small-ts. A single-point mutant (Val134Met) and a double-point mutant (Trp147Gly;Leu152 Pro) of small-t exhibited 3-fold and 5-fold lower potencies in inhibiting PP2A activity. respectively. This suggests that the region around amino acids between 134 and 152 of small-t might be important in regulating the enzyme activity of PP2A.

Endometrial Cell Culture: Isolation, Characterization, and Immortalization

홍인선,김석현,궁미경,전진현,이영순,강경선,Hong, In-Sun,Kim, Seok-Hyun,Koong, Mi-Kyoung,Jun, Jin-Hyun,Lee, Yong-Soon,Kang, Kyung-Sun The Korean Society for Reproductive Medicine 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.4

목 적: 본 실험의 목적은 자궁내막세포를 분리 및 배양법 확립과 함께 불멸화 시키는 것이다. 방 법: 자궁내막에서 상피세포(epithelial cells)와 기질세포(stromal cells)의 분리는 Satyawaroop 등(1979)의 방법에 기초를 두었다. 자궁내막에서 상피세포와 기질세포의 순수 분리도를 확인하고, 불멸화된 기질세포에서 SV40 large T antigen을 확인하기 위하여 면역형광 염색(immunocytochemistry)과 Western blot 기법을 이용하였다. 정상 기질세포의 경우 subconfluence (60%) 상태에서 transfection을 진행하였다. 순수 분리된 plasmid DNA와 Qiagen 사의 superfect를 이용하여 transfection을 실시하였다. 결 과: 본 연구에서 우리는 두 가지 형태의 자궁내막 세포의 분리 및 배양에 성공하였다. 상피세포는 다면체의 형태를 띠며, 선(grandular)조직의 조각으로부터 나선형으로 자란다.기질 세포는 길쭉한 형태를 띠며, 상피세포에 비해 오래 살고, 빠르게 증식하여 나란한 형태로 배열된 세포 다발(cell bundle)을 형성한다. 이렇게 분리된 세포들은 95%의 균질성을 보였으며, 면역형광염색과 western blot을 통해 확인 하였다. 한편 SV40(Simian Virus 40) large T 항원을 암호화 하고 있는 염기 서열을 포함한 플라스미드 벡터로 안정적인 트랜스펙션을 시킴으로써 불멸화 된 자궁내막의 기질 세포주를 확립하였다. 불멸화 된 세포는 그 세포가 유래한 정상의 세포와 동일한 표현형을 가지고 있었다. 결 론: 본 연구에서, 우리는 자궁내막에서 상피세포(epithelial cells)과 기질세포(stromal cells)를 분리하여 배양법을 확립하였다. 동시에 SV40 large T antigen을 이용하여 불멸화된 세포주를 확립하였다. 이렇게 확립된 세포주는 자궁의 생리작용 연구 및 자궁내막증(Endometriosis)과 자궁암(Endometrial cancer) 등과 같은 여러 자궁관련 질병 연구에 많은 도움이 될 것으로 사료된다.

Well-Differentiated Papillary Mesothelioma of the Tunica Vaginalis: A Case Study and Review of the Literature

Seyda Erdogan,Arbil Acikalin,Handan Zeren,Gulfılız Gonlusen,Suzan Zorludemir,Volkan Izol 대한병리학회 2014 Journal of Pathology and Translational Medicine Vol.48 No.3

Well-differentiated papillary mesothelioma is an uncommon tumor of the testes that usually presents as a hydrocele. Here, we present the case of one patient who did not have a history of asbestos exposure. The tumor was localized in the tunica vaginalis and was composed of three pedunculated masses macroscopically. Microscopically, branching papillary structures with focal coagulative necrosis were present. In addition to immunohistochemistry, simian virus 40 DNA was also tested by polymerase chain reaction. This report presents one case of this rare entity, its clinical and macroscopic features, and follow-up results.