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- 주제어 : Sargassum fusiformis (검색결과 4 건)
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구재근 ( Jae Geun Koo ),조길석 ( Kil Suk Jo ),도정룡 ( Jeong Ryong Do ),박진희 ( Jin Hee Park ),양차범 ( Cha Bum Yang ) 한국수산학회 1995 한국수산과학회지 Vol.28 No.5
톳과 모자반으로부터 fucoidan을 추출, 정제하여 그 성질을 분석, 검토하였다. Fucoidan의 추출 조건은 pH 2.0의 산성 추출액으로 65℃에서 1시간씩 3회 추출을 하고 cethylpyridinum chloride를 사용하여 부분 정제하였다. 부분 정제 fucoidan의 수율은 톳 2.51%, 모자반 1.56%였다. DEAE-Sephadex A-25 이온 교환 칼럼으로 부분 정제 fucoidan을 분획한 결과 톳, 모자반 모두 3종의 획분으로 분획되었고, 각 획분은 전기 영동상에서 2종 이상의 band가 검출되어 불균일함을 알 수 있었다. 분획한 주획분을 다시 알콜 분별 침전법으로 재분획한 결과 톳은 에타놀농도 60~70%에서 침전된 획분(P-70)이, 모자반은 에타놀농도 60~65%에서 침전된 획분(P-65)이 cellulose acetate 전기 영동 및 젤 여과 크로마토그라프상에서 각각 균일성을 나타내었다. 균일하게 정제 된 톳과 모자반 fucoidan의 fucose: galactose: 황산기의 mole 조성비는 각각 1:0.66:2.74와 1:0.24:1.46였으며, 분자량은 톳은 26,000, 모자반은 105,000이었다. Fucoidans were isolated from Hizikia fusiformis and Sargassum fulvellum and characterized on their chemical properties. Crude fucoidans were extracted at 65℃ for 1hr with acid solution of pH 2.0, and cetylpyridinum chloride was used for partial purification. The yields of partially purified fucoidans were 2.51% for H. fusiformis, and 1.56% for S. fulvellum, respectively. The partially purified fucoidans were separated into 3 fractions by DEAE-Sephadex A-25 ion exchange column chromatography and the major fractions were refractionated with fractional precipitation with ethanol. 60~70% ethanol precipitated fraction(P-70) of H. fusiformis and 60~65% ethanol precipitated fraction(P-65) of S. fulvellum turned out to be homogeneous by cellulose acetate electrophoresis and gel filtration chromatography. The molar ratios of fucose, galactose, and sulfate in the purified fucoidans were 1:0.66:2.74 for H. fusiformis and 1:0.24:1.46 for S. fulvellum. The averaged molecular weights of the purified fucoidans from H. fusiformis and S. fulvellum were 26,000 and 105,000, respectively.
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Yu-Lin Dai,Yun-Fei Jiang,Yu-Hang Nie,Yu-An Lu,Min-Cheol Kang,You-Jin Jeon 제주대학교 해양과학연구소 2020 해양과환경연구소 연구논문집 Vol.44 No.-
In our previous study, the anticancer effect of the active fucoidan (JHCF4) isolated from acid-processed Sargassum fusiformis was evaluated. In this study, the liver-protective effects of JHCF4 against ethanol-induced Chang liver cell damage and apoptosis-related responses were investigated. Furthermore, the low cytotoxicity and high cell viability of JHCF4 against ethanol-induced cell damage, as well as its protective effect against ethanol-induced cell apoptosis, were observed via nuclear staining with Hoechst 33342 in Chang liver cells. Additionally, the treatment of the 72 h post-fertilization (hpf) zebrafish model with JHCF4 increased the ethanol-stimulated survival rates as well as decreased oxidative stress, lipid peroxidation, and cell death levels. JHCF4 was found to significantly decrease steatosis production in the 128 hpf zebrafish model by Oil Red O staining, as well as attenuate the malondialdehyde and increase the glutathione contents, compared with the untreated group. These results demonstrate that JHCF4 has a potential hepato-protective effect against ethanol-induced damage both in vitro and in vivo.
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서정호,감상규,오영희,이동환,이민규 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1
Lead sorption performances by biomass of nonliving, dried marine brown algae Undaria pinnatifida, Hizikia fusiformis, and Sargassum fulvellum used as biosorbent materials were investigated. As the amount of biosorbent materials added was increased, the lead removal by biosorbent materials increased but the lead biosorption capacities decreased. However, with increasing initial lead concentration the lead biosorption capacities by the biosorbent materials increased but lead removal efficiencies decreased. In the range of initial lead concentration(C_0) 10-500 mg/L the lead biosorption capacities and removal efficiencies by the biosorbent materials increased with increasing pH. Among the biosorbent materials used in this study, the lead biosorption capacities decreased in the following sequence: U. pinnatifida $gt; H. fusiformis $gt; S. fulvellum. The lead biosorption by biosorbent materials were expressed by the Langmuir isotherm better than the Freundlich isotherm. The biosorption rate could be expressed by the first order reaction rate equation for initial lead concentration like that r_ad = 0.288C_0 for U. pinnatifida, r_ad = 0.255C_0 for H. fusiformis, and r_ad = 0.161C_0 for S. fulvellum.
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해조류 추출물과 페놀성화합물의 in vitro 및 in vivo 간보호활성
최재수,송상호,최명락,김광용,최종원,박종철,Choi, Jae-Sue,Song, Sang-Ho,Choi, Myeong-Rak,Kim, Kwang-Young,Choi, Jong-Won,Park, Jong-Cheol 한국생약학회 1997 생약학회지 Vol.28 No.4
The methanol extracts of some marine algae were tested for investigating the effects on the formation of lipid peroxide and the activities of free radical generating enzyme in vitro in bromobenzene-treated rat. The extracts of Enteromorpha compressa, Capsosiphon fulvescens, Gelidium amansii, Hizikia fusiformis, Sargassum siliquastrum and Sargassum thunbergii which decreased the formation of lipid peroxide, inhibited the activity of xanthine and aldehyde oxidases by adding of each extracts. Phloroglucinol isolated from Ecklonia stolonifera reduced bromobenzene-induced hepatic lipid peroxidation. This compound administered daily over one week before intoxication with bromobenzene did not affect the activities of aminopyrine N-demethylase, aniline hydroxylase and glu tathione S-transferase. Epoxide hydrolase activity was decreased by bromobenzene, which was restored by pretreatment of phloroglucinol, The results suggest that the bromobenzene-induced hepatic lipid peroxidation by phloroglucinol is reduced by enhancing the activity of epoxide hydrolase.
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