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성범죄 증거물에서 Y-STR Genotyping을 위한 PowerPlex®Y23 Kit의 법과학적 적용 가능성 검토
이해용,황인관,박민선,정규식,박선희,강필원,조남수,최동호 한국과학수사학회 2015 과학수사학회지 Vol.9 No.3
성범죄에 있어 남성의 흔적을 찾기 위한 Y-STR형 분석은 다량의 여성 DNA가 오염된 시료에서 큰 의미가 있다. 최근, 출시된 PowerPlex®Y23 kit (PPY23 kit)와 기존에 사용되었던 AmpFLSTR®Y-filerTM kit (Y-filer kit)의 민감도와 검출한계를 비교하고 두 제품과의 민감도 비교를 위하여 AmpFLSTR®Identifiler PLUS kit (ID PLUS kit)를 추가하 였다. 순차적으로 희석된 시료들을 이용하여 각 kit에서 전체 유전자형이 안정적으로 확인되는 DNA 시료의 최소량을 비교한 결과, ID PLUS kit와 Y-filer kit는 0.063 ng 그리고 PPY23 kit는 0.031 ng에서 안정적인 유전자형을 확인할 수 있는 민감성을 보였다. 다량의 여성 DNA에 미량의 남성 DNA가 혼합된 경우, 남성 특이적인 Y-STR 유전자형을 확보할 수 있는 최대 비율(남성 DNA:여성 DNA)을 확인해 보았다. 그 결과, Y-filer kit는, 1:2,000 혹은 1:4,000까지 남성의 유전자형을 안정적으로 확인할 수 있는 반면에 PPY23 kit는 그 보다 더 높은 비율인 1:20,000 배까지 안정적으로 남성의 유전자형을 확보할 수 있었다. PPY23 kit의 이러한 높은 민감도는 미량의 남성 DNA에 다량의 여성의 DNA가 혼합된 여성의 팬티나 질 내용물과 같은 현장증거물에서도 동일하게 확인되었다. 그러나, 두 남성의 DNA가 서로 혼합된 경우에는 PPY23 kit와 Y-filer kit 사이에서 민감성의 차이를 확인할 수 없었다. 이러한 결과들은 현장 증거물에서 PPY23 kit가 Y-filer kit를 충분히 대체할 수 있을 뿐 아니라, 미량의 DNA 시료에 대해 유용성이 높아 적용이 유용할 것으로 사료된다.
Genetic polymorphisms of eight X-STR loci of Mentype Argus X-8 kit in Koreans
Yoo, S.Y.,Cho, N.S.,Park, S.W.,Hwang, J.H.,Kim, J.N.,Chung, K.W.,Han, M.S. Elsevier 2011 Forensic science international. Genetics. Suppleme Vol.3 No.1
We have examined 45 Korean haplotype transfers in 41 families at eight X-linked STRs (DXS10135 and DXS8378 in linkage group 1, DXS7132 and DXS10074 in linkage group 2, HPRTB and DXS10101 in linkage group 3, DXS10134 and DXS7423 in linkage group 4) of Mentype Argus X-8 kit. The allele frequencies and haplotype frequencies within the four linkage groups were determined for females and males, respectively. Deviation from Hardy-Weinberg expectation could not be detected (p<0.05). No recombination was observed within each 4 linkage groups, however, six cases of mutation were founded in DXS10135, DXS10101, and DXS10134. Details of X-STR haplotype study in Koreans would be useful in kinship testing and forensic applications.
Forensic Genetic Analysis for the PowerPlex-16 System in the Korean Population
Han Jun Jin,Kyoung Don Kwak,Seung Bum Hong,Youl Hey Cho,Myun Soo Han,Wook Kim 한국유전학회 2007 Genes & Genomics Vol.29 No.4
We have analyzed 15 short tandem repeat (STR) markers included the Power Plex-16 (Promega) kit in a sample of 165 unrelated individuals from Korea. Allele frequencies and forensic parameters were calculated to provide an expanded and reliable forensic database. The Exact Test demonstrated that all loci surveyed here were found to be no deviation from Hardy-Weinberg equilibrium. Statistical analyses based on allele frequencies of the 15 STR loci show that twelve Eurasian populations tested can be classified into two distinct clusters; the Koreans appear to have the most genetic affinity with the Japanese of the East Asian cluster but tend to be different from the European cluster. The calculated parameters showed Penta E to be the most valuable marker from the STR loci studied. The combined probability of match calculated from 15 STR loci was 2.19 × 10-17, which is highly informative. Our data, therefore, can be used to extend the results obtained with other STRs, as well as provide valuable information for forensic and population genetic studies in the Korean population.
Forensic and population genetic analyses of the GlobalFiler STR loci in the Mongolian population
최은지,박기원,이양한,남윤형,Ganbold Suren,Uyanga Ganbold,김지애,김소연,김혜민,김기철,김욱 한국유전학회 2017 Genes & Genomics Vol.39 No.4
We have analyzed 24 loci including autosomal and Y-chromosomal short tandem repeats (STRs), Y-indel, and sex-determining marker in a sample of 267 unrelated individuals from the Mongolian population using the GlobalFiler™ PCR Amplification Kit to provide an expanded and more reliable forensic database. Khalkh among 15 Mongolian minor-groups accounts for about 80% of the entire Mongolian population. A total of 267 different DNA profiles were found in this work. The highest gene diversity was observed in the SE33 (0.9376) locus, and the lowest value was found in the TPOX (0.6142) locus. Although individual power of discrimination estimates varied at the studied loci, combined probability of match from the 21 STR loci was estimated to be 1.139 × 10−24, which is highly informative. Based on the results of pairwise FST genetic distances and multi-dimensional scaling plot showed that Mongolians were clustered into Europeans and Asians, although Mongolia is geographically located in Northeastern Asia. Thus, the present survey of the Mongolian population may help establish a comprehensive reference database for forensic and population genetic analyses.
Ahmed M.A. Ibrahim,Lamia A. ALRAKAN,Saleh Ahmed ALAIFAN 한국곤충학회 2015 Entomological Research Vol.45 No.5
Female mosquitoes feed on human blood, which can be collected to analyze human short tandem repeat (STR) sequences; these are specific to each human individual. Analysis of STRs might help in identification of a person found near a crime scene. Aedes aegypti and Culex pipiens mosquitoes fed on human blood were cultured at 18°C or 40°C (median temperature for summer and winter time in Riyadh governorate, Saudi Arabia) for 3, 6, 12, 24, 48 and 72 h. In A. aegypti, human DNA concentration was reduced with time at both temperatures. At 18°C, we obtained full STR profiles up to 48 h post feeding on human blood while none of the 16 loci were obtained at 72 h. At 40°C, we missed six sites at 12 h after blood sucking, 12 at 24 h, and 15 at 48 h and 72 h. In C. pipiens cultured at 18°C, full profiles were developed up to 48 h following blood feeding while we could not amplify five sites at 72 h. At 40°C, mortality among females was 50% at 24 h and 100% at both 48 h and 72 h; however, we had full profiles in all samples including dead insects. This research addressed the possibility of using mosquitoes in forensic research by DNA genotyping by changing the mosquito culturing temperature and mosquito genus. Our findings proved that different types of mosquito change the temporal pattern of STR analysis and showed that the mosquito culturing temperature affects the integrity of DNA for STR analysis.