Does treatment of Helicobacter Pylori Infection Reduce Gastric Precancerous Lesions?

Mansour-Ghanaei, Fariborz,Joukar, Farahnaz,Mojtahedi, Kourosh,Sokhanvar, Homayoon,Askari, Kourosh,Shafaeizadeh, Ahmad Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4

Background: Treatment of Helicobacter pylori (H. pylori) decreases the prevalence of gastric cancer, and may inhibit gastric precancerous lesions progression into gastric cancer. The aim of this study was to determine the effect of treatment on subsequent gastric precancerous lesion development. Materials and Methods: We prospectively studied 27 patients who had low grade dysplasia at the time of enrollment, in addition to dysplasia atrophic gastritis and intestinal metaplasia observed in all patients. All were prescribed quadruple therapy to treat H. Pylori infection for 10 days. Patients underwent endoscopy with biopsy at enrollment and then at follow up two years later. Biopsy samples included five biopsies from the antrum of lesser curvature, antrum of greater curvature, angularis, body of stomach and fundus. Results of these biopsies were compared before and after treatment. Results: Overall, the successful eradication rate after two years was 15/27 (55.6%). After antibiotic therapy, the number of patients with low grade dysplasia decreased significantly (p=0.03), also with reduction of the atrophic lesions (p=0.01), but not metaplasia. Conclusions: Treatment of H. pylori likely is an effective therapy in preventing the development of subsequent gastric premalignant lesions.

Scoring System and Management Algorithm Assessing the Role of Survivin Expression in Predicting Progressivity of HPV Infections in Precancerous Cervical Lesions

Indarti, Junita,Aziz, M. Farid,Suryawati, Bethy,Fernando, Darrell Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Background: To identify the risk factors and assess the role of survivin in predicting progessivity precancerous cervical lesions. Materials and Methods: This case-control study was conducted from October 2009 until May 2010. We obtained 74 samples, classified according to the degree of cervical intraepithelial neoplasia (CIN): 19 samples for CIN 1, 18 samples for CIN 2, 18 samples for CIN 3, and 19 samples as controls. Demographic profiles and risk factors assesment, histopathologic examination, HPV DNA tests, immunocytochemistry (ICC) and immunohistochemistry (IHC) staining for survivin expression were performed on all samples. Data was analyzed with bivariate and multivariate analysis. Results: Multivariate analysis revealed significant risk factors for developing precancerous cervical lesions are age <41 years, women with ${\geq}2$ sexual partners, course of education ${\geq}13$ years, use of oral contraceptives, positive high-risk HPV DNA, and high survivin expression by ICC or IHC staining. These factors were fit to a prediction model and we obtained a scoring system to predict the progressivity of CIN lesions. Conclusions: Determination of survivin expression by immunocytochemistry staining, along with other significant risk factors, can be used in a scoring system to predict the progressivity of CIN lesions. Application of this scoring system may be beneficial in determining the action of therapy towards the patient.

Screening of Precancerous Gastric Lesions by Serum Pepsinogen, Gastrin-17, Anti-Helicobacter Pylori and Anti-Caga Antibodies in Dyspeptic Patients over 50 years Old in Guilan Province, North of Iran

Mansour-Ghanaei, Fariborz,Joukar, Farahnaz,Rajpout, Yaghoub,Hasandokht, Tolou Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Background: The aim of this study was to investigate the value of serum gastric markers to differentiate between patients with precancerous lesions and nonatrophic chronic gastritis. Materials and Methods: Serum samples of 128 patients with dyspepsia who were candidates for endoscopic examination were tested for pepsinogen (PG I and PG II), PG I/II ratio, gastrin 17(G-17), anti-Helicobacter pylori (anti-H pylori ) and anti-CagA antibodies. Two sample t-tests, chi-square tests and Pearson's correlation analyses were used for analysis using SPSS (version 20). Results: PGI, PG I/II ratio values were decreased significantly in the precancerous lesion group (0.05, 0.001 respectively). The frequency of H pylori infection was significantly (p=0.03) different between the two groups ofthe study. Conclusions: We suggest PGI and the PG I/II ratio as valuable markers for screening of premalignant gastric lesions.

Adverse Effects of Preserved Vegetables on Squamous Cell Carcinoma of Esophagus and Precancer Lesions in a High Risk Area

Song, Qing-Kun,Zhao, Lin,Li, Jun,He, Yu-Ming,Jiang, Cui-Ping,Jiang, Hai-Dong,Qu, Chen-Xu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Introduction: Squamous cell carcinoma of esophagus (ESCC) is one of the most common cancers in China. Preserved vegetables are processed foods and consumed in high amounts in the high risk areas for ESCC. This study aimed to investigate the relationships of preserved vegetable consumption with ESCC and precancer lesions. Methods: Cases from Yanting cancer hospital with pathological diagnosis of primary cancer, along with controls and individuals diagnosed with precancer lesions by endoscopy with iodine staining were interviewed. Trained staff collected data on dietary habits 1 year before the interview. An unconditional logistic regression model was used to estimate odds ratios of preserved vegetable consumption for precancer lesions and cancer. Results: Adjusting for potential confounders, intake of preserved vegetables (OR=2.92, 95%CI 1.32~6.47) and longer intake period (OR=5.78, 95%CI 2.26~14.80) were associated with higher risk of ESCC. Compared with lowest intake frequency, the highest was associated with a 3.0-fold risk for precancer lesions and 3.59-fold risk for ESCC (both p<0.05). Conclusion: Consumption of preserved vegetables is a risk factor for esophageal lesions in high risk areas. The carcinogenicity of preserved vegetables needs investigation in further studies and the public health strategies for reducing the consumption might be initiated in high risk areas.

구강점막의 유두상 병소

윤정훈(Jung-Hoon Yoon) 대한치과의사협회 2012 대한치과의사협회지 Vol.50 No.12

Oral mucosal lesions with a papillary or verrucous surface are a heterogenous group of conditions with widely varying clinical behaviour. The etiology is variable and includes infective, traumatic or neoplastic processes. The majority of these lesions are precanceous lesion. Therefore, a biopsy is required to establish a diagnosis and the microscopic examination must be coordinated with the clinical findings. This paper reviews the clinical features and significance of papillary or verrucous lesions.

Feasibility of quantifying <i>SDC2</i> methylation in stool DNA for early detection of colorectal cancer

Oh, Tae Jeong,Oh, Hyun Il,Seo, Yang Yei,Jeong, Dongjun,Kim, Changjin,Kang, Hyoun Woo,Han, Yoon Dae,Chung, Hyun Cheol,Kim, Nam Kyu,An, Sungwhan BioMed Central 2017 CLINICAL EPIGENETICS Vol.9 No.1

<P><B>Background</B></P><P>Colorectal cancer (CRC) screening is the most efficient strategy to reduce disease-related mortality. Frequent aberrant DNA methylation is known to occur in selected genes and early during CRC development, which has emerged as a new epigenetic biomarker for early detection of CRC. Previously, we reported that we identified that CpG sites of <I>SDC2</I> were aberrantly methylated in tumor tissues of most CRC patients through comprehensive methylation analysis and demonstrated a high potential of quantification of <I>SDC2</I> methylation in blood for early detection of colorectal cancer. In this study, we aim to investigate the feasibility of quantifying <I>SDC2</I> methylation in stool DNA for the early detection of CRC. The objective of this study was to confirm a high frequency of <I>SDC2</I> methylation in tumor tissues at various stages of CRC and investigate the feasibility of a quantitative test for <I>SDC2</I> methylation in fecal DNA by highly sensitive and accurate real-time PCR for early detection of CRC.</P><P><B>Methods</B></P><P>Bisulfite-pyrosequencing assay was performed to measure the <I>SDC2</I> methylation status in tissue samples. For methylation analysis in stool DNA, a highly sensitive and accurate method was applied which implements consecutive two rounds of PCR consisting of unidirectional linear target enrichment (LTE) of <I>SDC2</I> and quantitative methylation-specific real time PCR (qMSP) for <I>SDC2</I>, named as me<I>SDC2</I> LTE-qMSP assay. Its limit of detection was 0.1% methylation (corresponding to ~ 6 copies in total ~ 6200 genome copies).</P><P><B>Results</B></P><P>Positive <I>SDC2</I> methylation was observed in 100% of primary tumors, 90.6% of adenomatous polyps, 94.1% of hyperplastic polyps, and 0% of normal tissues. <I>SDC2</I> methylation level also significantly (<I>P</I> < 0.01) increased according to the severity of lesions. In stool DNA test for <I>SDC2</I> methylation by LTE-qMSP comparing CRC patients with various stages (I to IV) (<I>n</I> = 50) and precancerous lesions (<I>n</I> = 21) with healthy subjects (<I>n</I> = 22), the overall sensitivity was 90.0% for detecting CRC and 33.3% for detecting small polyps, with a specificity of 90.9%.</P><P><B>Conclusions</B></P><P>Taken together, our result indicates that stool DNA-based <I>SDC2</I> methylation test by LTE-qMSP is a potential noninvasive diagnostic tool for early detection of CRC.</P>

Comparison of p16INK4a Immunocytochemistry with the HPV Polymerase Chain Reaction in Predicting High Grade Cervical Squamous Intraepithelial Lesions

Indarti, Junita,Fernando, Darrell Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Aim: To compare p16INK4a immunocytochemistry with the HPV polymerase chain reaction in predicting high grade cervical squamous intraepithelial lesions. Materials and Methods: This diagnostic case-control study was conducted from January 2010 until December 2010. We obtained 30 samples, classified according to the degree of cervical intraepithelial neoplasia (CIN): 11 samples for CIN 1, 9 samples for CIN 2, and 10 samples for CIN 3. HPV PCR, p16INK4a immunocytochemistry, and histopathological examination were performed on all samples. Statistical analysis was conducted using SPSS 20.0. Results: In predicting CIN 2-3, we found p16INK4a to have similar specificity and positive predictive value as HPV PCR (95%, 97.2% vs 96.7%), but better sensitivity (87.5% vs 72.5%) and negative predictive value (82.1% vs 67.6%). The most prevalent types of high-risk HPV in our study were HPV 33, 35, 58, 52, and 16. Conclusions: p16INK4a has better diagnostic values than HPV PCR and may be incorporated in the triage of ASCUS and LSIL to replace HPV PCR. Genotype distribution of HPV differs in each region, providing a challenge to develop HPV vaccines based on the epidemiology of HPV in that particular region.

위 전암성 병변에 대한 내시경적 추적 관찰

김규종,박선자,남지현,임현정,김지연,박무인,구자영 고신대학교 의학부 2005 高神大學校 醫學部 論文集 Vol.20 No.1

Background: The aims of this study were to investigate the endoscopic findings of precancerous lesions of stomach and to see whether or not these lesions could progress to the carcinoma. Method: May 2000 to February 2001, consecutive patients diagnosed as having chronic active gastrits (CAG), intestinal metaplasia (IM), dysplasia, adenoma by endoscopic biopsy were enrolled. A total of 359 patients were included in this study and endoscopically followed-up to Autust 2001. Results; Among all 33 patients (26 dysplasia, 7 dysplasia with adenoma) diagnosed as dysplasia by endoscopic biopsy, there were 6 chronic erosive gastritis (CEG), 6 chronic erosion, 17 adenoma, 4 R/O cancer. On follow-up endoscopic biopsy (17 patients), there were 3 IM, 4 dysplasia, 5 adenoma, 5 carcinoma. Among all 107 patients (53 IM, 38 IM with CAG, 14 IM with CEG, 1 IM with adenoma, 1 IM with dysplasia) diagnosed as IM by endoscopic biopsy, there were 52 CEG, 33 chronic erosion, 6 local lesion, 5 adenoma, 5 R/O cancer, 3 gastric polyp, 3 atrophy with IM> On follow-up endoscopic biopsy (25 patients), there were 2 chronic gastritis, 1 CEG, 3 CAG, 14 IM 3 dysplasia, 2 adenoma, 1 carcinoma. Among all 185 patients (147 CAG, 37 CAG with IM, 1 CAG with adenoma) diagnosed as CAG by endoscopic biopsy, there were 86 CEG, 62 chronic erosion, 4 local lesion, 10 adenoma, 16 R/O cancer, 4 gastric polyp, 2 IM, 1 chronic gastritis. On follow-up endoscopic biopsy (35 patients), there were 8 chronic gastritis, 3 CEG, 14 CAG, 5 IM, 2 dysplasia, 1 adenoma, 2 arcinoma. Among All 34 patiets (22 adenoma, 8 adenoma with low grade dysplasia, 2 adenoma with moderate grade dysplasia, 2 adenoma with high grade dysplasia) diagnosed as gastric adenoma by endoscopic biospy, there were 1 local lesion, 24 adenoma, 2 R/O cancer, 7 gastric polyp. On follow-up endoscopic biopsy (17 patients), there were 2 CEG, 2 low grade dysplasia, 1 high grade dysplasia, 4 adenoma 1 adenoma with dysplasia, 4 adenoma with low grade dysplasia, 2 adenoma with high grade dysplasia, I cancerous change. Conclusions: Althought it was short period, there were some of cancerous change in precancerous lesions at follow-up endoscopy. This study suggests that screening for precancerous lesions, is one method of improving the prognosis of gastric carcinoma and when the precancerous lesions are diagnosed on endoscopy, serial endoscopic follow-up should be done to find early gastric cancer.

위 전암성 병변에 대한 내시경적 추적 관찰

김규종,박선자,남지현,임현정,김지연,박무인,구자영 고신대학교(의대) 고신대학교 의과대학 학술지 2005 고신대학교 의과대학 학술지 Vol.20 No.1

Backgrounds : The aims of this study were to investigate the endoscopic findings of precancerous lesions of stomach and to see whether or not these lesions could progress to the carcinoma. Methods : May 2000 to February 2001, consecutive patients diagnosed as having chronic active gastritis (CAG), intestinal metaplasia (IM), dysplasia, adenoma by endoscopic biopsy were enrolled. A total of 359 patients were included in this study and endoscopically followed-up to Augus 2001. Results : Among all 33 patients (26 dysplasia, 7 dysplasia with adenoma) diagnosed as dysplasia by endoscopic biopsy, there were 6 chronic erosive gastritis (CEG), 6 chronic erosion, 17 adenoma, 4 R/O cancer. On follow-up endoscopic biopsy *17 patients), there were 3 IM, 4 dysplasia, 5 adenoma, 5 carcinoma. Among all 107 patients (53 IM, 38 IM with CAG, 14 IM with CEG, 1 IM with adenoma, 1 IM with dysplasia) diagnosed as IM by endoscopic biopsy, there were 52 CEG, 33 chronic erosion, 6 local lesion, 5 adenoma, 5 R/O cancer, 3 gastric polyp, 3 atrophy with IM. On follow-up endoscopic biopsy (25 patients), there were 2 chronic gastritis, 1 CEG, 2 CAG, 14 IM, 3 displasia, 2 adenoma, 1 carcinoma. Among all 185 patients (147 CAG, 37 CAG with IM, 1 CAG with adenoma) diagnosed as CAG by endoscopic biopsy, there were 86 CEG, 62 chronic erosion, 4 local lesion, 10 adenoma, 16 R/O cancer, 4 gastric polyp, 2 IM, 1 chronic gastritis. On follow-up endoscopic biopsy (35 patients), there were 8 chronic gastritis, 3 CEG, 14 CAG, 5 IM, 2 dysplasia, 1 adenoma, 2 carcinoma. Among all 34 patients (22 adenoma, 8 adenoma with low grade dysplasia, 2 adenoma with moderate grade dysplasia, 2 adenoma with high grade dysplasia) diagnosed follow-up endoscopic biopsy (17 patients), there were 2 CEG, 2 low grade dysplasia, 1 high grade dysplasia, 4 adenoma, 1 adenoma with dysplasia, 4 adenoma with low grade dysplasia, 2 adenoma with high grade dysplasia, 4 adenoma, 1 adenoma with dysplasia, 4 adenoma with low grade dysplasia, 2 adenoma with high grade dysplasia, 1 cancerous change. Conclusions : Although it was short period, there were some of cancerous change in precancerous lesions at follow-up endoscopy. This study suggests that screening for precancerous lesions, is one method of improving the prognosis of gastric carcinoma and when the precancerous lesions are diagnosed on endoscopy, serial endoscopic follow-up should be done to find early gastric cancer.

Cervical Precancerous Lesions and Cancer among Patients in the Gynaecology Outpatient Department at a Tertiary Hospital in South Africa

Hank, Edward,Hoque, Muhammad Ehsanul,Zungu, Lindiwe Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Background: The purpose of this study was to determine proportional rates of cervical precancerous lesions and cancer, and associations with socio-demographic variables, HIV status, and CD4 cell count. Materials and Methods: A retrospective comparative study was conducted targeting the medical records of all women over age 18 that were diagnosed histologically through colposcopy with cervical precancerous lesions and cancer from 1 December 2011 to 30 November 2012. Results: A total of 313 patient records were used for data analysis. The average age was 39.1 (SD=2.04) years. More than a third (37.1%) of the patients had CIN III or cervical cancer. There was a significant association between age, CD4 cell count, HIV status, and cervical lesions (p<0.05). Age was found to be an influential predictor of cervical lesions (OR=0.67, 95%CI: 0.46-0.98). Conclusions: There exists an association between age and cervical lesions, with presentation of cervical precancerous lesions and cervical cancer at a younger age among HIV reactive patients. The National Guidelines should be brought in line with the National HIV Counseling and Testing Policy to offer Pap smears to all sexually active women that test HIV reactive during routine HIV Testing.