The First Report of Two Species of Polyporus (Polyporaceae, Basidiomycota) from South Korea

Jin Sung Lee,Eun Ju Woo,Young Woon Lim,김재진,Kyoung Hee Oh 한국미생물학회 2010 The journal of microbiology Vol.48 No.6

Based on morphological examination, two species of Polyporus, P. dictyopus, and P. tuberaster, were identified,which constitutes the first record of these species in South Korea. To confirm their affinity within the genus Polyporus, the phylogenetic relationships of Polyporus and allied genera were established from nuclear large subunit ribosomal DNA (nLSU rDNA) sequences, and a morphological diagnostic key is presented to clarify the Korean species of Polyporus.

작은노란대구멍장이버섯 균사체 배양액의 항염증 활성

홍혜현,김민경,이유정,박태진,김창무,지원재,김승영 한국응용생명화학회 2024 Journal of Applied Biological Chemistry (J. Appl. Vol.67 No.-

Polyporus parvovarius is a species of fungus in the genus Polyporus. Recently, studies are shown that Polyporus has been evaluated to have anti-bacterial and anti-tumor. However, Anti-inflammatory effect of P. parvovarius mycelial culture filtrate (Pp) has not reported yet. Therefore, in this study, the lipopolysaccharide-induced RAW264.7 inflammation model was used to evaluate the anti-inflammatory activity of Pp cultured in four types of culture media. As a result, Pp_DY inhibited nitric oxide, prostaglandin E2, and inflammatory cytokines including tumor necrosis factor-α, interleukin-6, interleukin-1β at non-toxic concentrations. In addition, Pp_DY significantly inhibited inducible nitric oxide synthase and cyclooxygenase-2 on lipopolysaccharidetreated RAW 264.7 macrophages. In conclusion, it was suggested that Pp_DY has the potential to be used as a mushroom resources with anti-inflammatory.

겨울우산버섯의 원형질체 분리와 유전자 형질전환

유선화,김명길,Ryu, Sun-Hwa,Kim, Myung-Kil 한국미생물학회 2014 미생물학회지 Vol.50 No.4

본 연구에서는 백색부후균인 겨울우산버섯의 원형질체 분리 조건과 유전자 형질전환 방법을 확립하였다. 겨울우산버섯 균사에 세포벽 분해효소로 0.5% Usukizyme을 처리하여 ml당 $1{\times}10^7/ml$개의 원형질체를 확보할 수 있었다. 형질전환체의 선별을 위해 hygromycin에 대한 저항성을 갖는 유전자(hph)를 선택표지로 이용하여 형질전환용 벡터(pHYgpt)를 제작하였다. 40% polyethylene glycol 용액과 aurintricarboxylic acid와 heparin, supermidine을 첨가하여 형질전환을 수행 한 결과 벡터 $1{\mu}g$ 당 100-160개의 수율로 형질전환체를 얻었다. 도입된 벡터는 hph 유전자의 PCR 증폭을 통해 형질전환체의 염색체내에 삽입되었음을 확인하였다. 이러한 결과는 polyporus 속의 유전자를 활용한 새로운 균주 개발에 유용한 기술이 될 것이다. This experiment was undertaken to investigate proper conditions for protoplast isolation and genetic transformation of the white rot fungi, Polyporus brumalis. The protoplasts were formed from mycelia at a frequency of $1{\times}10^7/ml$ with 0.5% Usukizyme. The transformation vector (pHYgpt) was constructed using hygromycin resistance gene (hph) for the selectable maker. The yield was 100-160 transformants/${\mu}g$ DNA in a transformation mediated by 40% polyethylene glycol solution with aurintricarboxylic acid, heparin and supermidine. The genomic integration of the pHYgpt was confirmed by hph-specific PCR and the expected amplified band appeared only in the transformants. These results could be an efficient tool in gene engineering of the genus polyporus.

마황, 상륙 및 저령의 독성평가를 위한 성분분석 및 안정성 시험

뉴엔티퐁타오,트란만흥,토다오쿵,허정임,곽승준,김지명,강태석,이제현,우미희,최재수,강삼식,배기환,민병선 한국생약학회 2010 생약학회지 Vol.41 No.2

A simple and reliable reverse phase HPLC method was developed to determine pharmacologically active marker compounds of Ephedrae Herba, Phytolaccae Radix and Polyporus. The stability test of water-extract of three natural medicines were examined for six months. However, no significant change in the content of the marker compounds of each extract observed during the time of investigation.

rDNA의 ITS 부위 염기서열 분석에 의한 겨울우산버섯 (Polyporus)속 균주의 유전적인 유연관계 분석

이찬중,전창성,정종천,공원식 한국버섯학회 2012 한국버섯학회지 Vol.10 No.1

This study was carried to identify a correct species and asses genetic diversity within the same species of Polyporus spp. preserved in Division of applied Microbiology. Contaminated isolates showed different growth rates, morphology and color of hyphae. We have reconstructed the phylogenetic tree of a select group of Polyporus spp. using nucleotide sequences of the internal transcribed spacer region(ITS) region. The phylogenetic tree was constructed by using the neighbor-joining method. PELF primers of 20-mer were used to assess genetic diversity of preserved isolates. Sequence analysis showed that three strains were different species and four strains were identified completely different nomenclature. According to the analysis of ITS sequences, the genus Polyporus clustered into five distinct group, most of which correlated with species-groups identified by RAPD method. Four isolates included strain PM02 showed high similarity with P. arcularius, four isolates included strain PM03 high similarity with P. alveolaris, three isolates included strain PM01 high similarity with P. tuberaster, and PM 06 and PM04 high similarity with P. brumalis and P. squamossus. Isolates were collected in the United States(PM10, PM11) was identified as P. alveolarius and P. arcularius. RAPD analysis of genetic polymorphisms of genus Polyporus showed a very different band patterns. As the result of RAPD and ITS region sequences analysis for preserved isolates, it seems likely that 6 isolates of Polyporus spp. may be need to reclassify or eliminate from preserved catalogue.

Identification of Metabolites from Phenanthrene Oxidation by Phenoloxidases and Dioxygenases of Polyporus sp. S133

( Hadibarata Tony ),( Sanro Tachibana ),( Muhamad Askari ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.3

Phenanthrene degradation by Polyporus sp. S133, a new phenanthrene-degrading strain, was investigated in this work. The analysis of degradation was performed by calculation of the remaining phenanthrene by gas chromatography-mass spectrometry. When cells were grown in phenanthrene culture after 92 h, all but 200 and 250 mg/l of the phenanthrene had been degraded. New metabolic pathways of phenanthrene and a better understanding of the phenoloxidases and dioxygenase mechanism involved in degradation of phenanthrene were explored in this research. The mechanism of degradation was determined through identification of the several metabolites; 9,10-phenanthrenequinone, 2,2`-diphenic acid, salicylic acid, and catechol. 9,10-Oxidation and ring cleavage to give 9,10-phenanthrenequinone is the major fate of phenanthrene in ligninolytic Polyporus sp. S133. The identification of 2,2`-diphenic acid in culture extracts indicates that phenanthrene was initially attacked through dioxigenation at C9 and C10 to give cis-9,10-dihydrodiol. Dehydrogenation of phenanthrene-cis-9,10-dihydrodiol to produce the corresponding diol, followed by ortho-cleavage of the oxygenated ring, produced 2,2`-diphenic acid. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase, and 2,3-dioxygenase) produced by Polyporus sp. S133 was detected during the incubation. The highest level of activity was shown at 92 h of culture.

Transformation of Terpene Synthase from Polyporus brumalis in Pichia pastoris for Recombinant Enzyme Production

( Ji-eun An ),( Su-yeon Lee ),( Sun-hwa Ryu ),( Myungkil Kim ) 한국목재공학회 2018 목재공학 Vol.46 No.4

Terpenoids have a wide range of biological functions and have extensive applications in the pharmaceutical, cosmetic, and flavoring industry. The white-rot fungus, Polyporus brumalis, is able to synthesize terpenoids via terpene synthase, which catalyzes an important step that forms a large variety of sesquiterpene products from farnesyl pyrophosphate (FPP). To improve the production of sesquiterpenes, the terpene synthase gene was isolated from Polyporus brumalis and was heterologously transformed into a Pichia pastoris strain. The open reading frame of the isolated gene (approximately 1.2 kb) was inserted into Pichia pastoris to obtain a recombinant enzyme. Five transformants were obtained and the expression of terpene synthase was analyzed at the transcript level by reverse transcription PCR (polymerase chain reaction) and at the protein level by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Expression of the terpene synthase gene product was elevated in the transformants and as expected the molecular weight of the protein was approximately 45 kDa. These recombinant enzymes have potential practical applications and future studies should focus on their functional characterization.

Bioremediation of Crude Oil by White Rot Fungi Polyporus sp. S133

( Kristanti Risky Ayu ),( Tony Hadibarata ),( Tadashi Toyama ),( Yasuhiro Tanaka ),( Kazuhiro Mori ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.9

The bioremediation potential of crude oil by Polyporus sp. S133 pre-grown in wood meal was investigated in two separate experiment trials; liquid medium and soil. The effect of three nutrients (glucose, polypeptone, and wood meal), oxygen flow, and some absorbent on the efficiency of the process was also evaluated. Degradation of crude oil in soil was significantly increased with an addition of oxygen flow and some absorbent (kapok and pulp). The highest degradation rate of crude oil was 93% in the soil with an addition of 10% kapok. The present study clearly demonstrates that, if suitably developed, Polyporus sp. S133 could be used to remediate soil contaminated with crude oil.

사람 모유두세포에서 PI3K/Akt와 Wnt/β-catenine 신호전달을 경유한 저령추출물의 세포증식 효과

강리아민주,강석종,문연자 대한본초학회 2024 大韓本草學會誌 Vol.39 No.1

Objectives : Polyporus umbellatus is a medicinal mushroom that has been used for over thousands years in Chinese medicine as a powerful diuretic to relieve fluid retention and edema. Dermal papilla is located at the bottom of the hair follicle and connected to the blood vessels where it gets the nutrients and oxygen to nurture hair follicle. This study examined the mechanism through which the ethanol extract of Polyporus umbellatus (EPU) promoted the proliferation of human dermal papilla cells (HHDPCs). Methods : To estimate the proliferative effects of EPU on HHDPCs, cell viability was estimated by thiazolyl blue tetrazolium bromide (MTT) assay. Western blotting was used to investgate the activation of ERK, phosphoinositide 3-kinase (PI3K)/Akt, β-catenin, GSK-3β and heme oxygenase-1 (HO-1). Cells were treated with inhibitors of ERK and Akt prior to EPU treatment. Results : EPU promoted the proliferation of HHDPCs and the phosphorylation of ERK and Akt in dose dependent manner. However, the proliferative effect of EPU on HHDPCs was inhibited by pre-treatment of ERK inhibitor (PD98059) and Akt inhibitor (LY294002). Furthermore, EPU respectively stimulated the protein expression of β- catenin and phosphorylated GSK-3β. EPU significantly increased the protein expression levels of proliferation and cytoprotection related genes such as Bcl-2, SIRT-1, and HO-1 in cells. Conclusion : This results suggest that EPU promoted the proliferation of HHDPCs via activating PI3K/Akt and Wnt/ β-catenin signaling pathway in HHDPCs.

Biosynthesis of Eudesmane-type Sesquiterpenoids by The Wood-rotting Fungus, Polyporus brumalis, on Specific Medium, including Inorganic Magnesium Source

Su-yeon Lee,Sun-hwa Ryu,In-gyu Choi,Myungkil Kim 한국목재공학회 2016 목재공학 Vol.44 No.2

Fungi, such as the wood-rotting Polyporus brumalis, are excellent sources of pharmaceutically interesting natural products such as sesquiterpenoids. In this study, we investigated the biosynthesis of P. brumalis sesquiterpenoids on modified medium. Ten additional species of white rot fungi were inoculated in medium containing nutrients such as C6H12O6, C4H12N2O6, KH2PO4, MgSO4, and CaCl2 at 28℃ for 25 days. After 10 days of incubation, eudesmane-type sesquiterpenes, β-eudesmane and β-eudesmol, were only synthesized during the growth phase of P. brumalis. Experiments excluding one nutrient at a time were conducted to determine the effects of inorganic nutrients on sesquiterpene biosynthesis. In conclusion, GC-MS analysis showed that biosynthesis of sesquiterpenes was differentially regulated by inorganic nutrients such as MgSO4, C4H12N2O6, and KH2PO4. We found MgSO4 supplementation to be vital for eudesmane-type sesquiterpene biosynthesis in P. brumalis; nitrogen (C4H12N2O6) and phosphate (KH2PO4) inhibited the synthesis of P. brumalis metabolites. Magnesium is a known cofactor of sesquiterpene synthase, which promotes β-eudesmol synthesis. To mechanistically understand eudesmane-type sesquiterpene biosynthesis in P. brumalis, further research into the genes regulating the dynamics of such biosynthesis is warranted.