한국산 양귀비과 식물에 관한 본초학적 연구

정종길 ( Jong-gil Jeong ) 대한본초학회 2016 大韓本草學會誌 Vol.31 No.5

Objective : For the purpose of developing Korean herbology of the plants to Papaveraceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article. Methods : Books and research papers about Herbology which published at home and abroad were examined. Results : A list was made about Papaveraceae plant which were cultivated or grew naturally in Korea, after investigated the data on domestic and foreign Papaveraceae plants. Out of those lists, serviceable plants were selected and with those available plants, their distributions were analysed and parts which can be used as medicines were divided into 3 categories such as oriental medicine name, scientific name and non-official name. Papaveraceae’s properties flavor, channels they use, effects, and toxicity were also noted. Papaveraceae (grew in Korea) were divided into 4 classes with 8 species. Out of those, 3 classes with 4 species were found serviceable which indicates 50% of all. Out of all 8 species of Papaveraceae family, Papaver genus were found 4 species, which were shown the most. Among 3 genus of 4 species, medicinal plants were all one species each. Out of all serviceable parts in Papaveraceae, Radix and Herba, Etc parts took first place as 2 species. There are toxic, the 3 species and 6 parts survey said. Conclusion : There were totaled to 4 genera and 8 species in Papaveraceae in Korea and among them medicinal plants are 3 genera, 4 species, some 50% in total.

The First Step of Biotechnological Approaches for Alkaloid Biosynthesis in Papaveraceae: In vitro Plant Regenerations

Park, Sang-Un,Yu, Chang-Yeon,Chae, Young-Am The Korean Society of Medicinal Crop Science 2004 韓國藥用作物學會誌 Vol.12 No.5

Alkaloid producing species of plants have long been a major component of the medicinal social and magico-religious aspects of human culture. A diverse array of biological activities has been attributed to different alkaloids including numerous members of benzylisoquinoline family of alkaloids. For biotechnological approaches of alkaloid biosynthesis in poppy family, plant regeneration protocol through somatic embryogenesis or shoot organogenesis is a first step. This paper describes the methods and applications of plant regeneration of poppy family.

The First Step of Biotechnological Approaches for Alkaloid Biosynthesis in Papaveraceae: In vitro Plant Regenerations

박상운 한국약용작물학회 2004 韓國藥用作物學會誌 Vol.12 No.5

Alkaloid producing species of plants have long been a major component of the medicinal, social and magico-religious aspects of human culture. A diverse array of biological activities has been attributed to different alkaloids including numerous members of benzylisoquinoline family of alkaloids. For biotechnological approaches of alkaloid biosynthesis in poppy family, plant regeneration protocol through somatic embryogenesis or shoot organogenesis is a first step. This paper describes the methods and applications of plant regeneration of poppy family.

The First Step of Biotechnological Approaches for Alkaloid Biosynthesis in Papaveraceae: In vitro Plant Regenerations

Sang-Un Park,Chang-Yeon Yu,Young-Am Chae 韓國藥用作物學會 2004 한국약용작물학회지 Vol.12 No.5

Alkaloid producing species of plants have long been a major component of the medicinal social and magico-religious aspects of human culture. A diverse array of biological activities has been attributed to different alkaloids including numerous members of benzylisoquinoline family of alkaloids. For biotechnological approaches of alkaloid biosynthesis in poppy family, plant regeneration protocol through somatic embryogenesis or shoot organogenesis is a first step. This paper describes the methods and applications of plant regeneration of poppy family.

Phylogenetic relationships of Coreanomecon (Papaveraceae: Papaveroideae), an endemic genus in Korea, using DNA sequences

윤나래,오상훈 한국식물분류학회 2018 식물 분류학회지 Vol.48 No.4

Coreanomecon is a monotypic and endemic genus in Korea, distributed mainly in the southern regions. Coreanomecon is morphologically similar to Hylomecon by producing red latex, easily distinguished from Chelidonium, which produces yellow latex. Coreanomecon were merged into Hylomecon or Chelidonium depending on the authors. To understand the phylogenetic relationship of Coreanomecon, DNA sequences of chloroplast rbcL and matK and nuclear Internal Transcribed Spacer (ITS) regions were determined from the spe- cies of Papaveroideae (Papaveraceae) in Korea and analyzed with the Maximum Parsimony and Bayesian meth- ods. Phylogenetic analyses of Papaveroideae suggest that Coreanomecon is sister to the clade of Chelidonium and Stylophorum in the ITS data and that it is sister to Hylomecon in the chloroplast (cpDNA) data. A con- straining analysis using the Shimodaira-Hasegawa test (S-H test) suggested that the ITS data do not reject the sister relationship of Coreanomecon and Hylomecon. The S-H test also suggested that the cpDNA data is com- patible with the placement of Coreanomecon as a sister to the clade of Chelidonium and Stylophorum. Although the conflicting phylogenetic results may stem from insufficient phylogenetic signals, they may also be associ- ated with hybridization between Hylomecon and an ancestor of Stylophorum and Chelidonium. The results of this study suggest that Coreanomecon is a distinct lineage as an endemic genus, supporting the morphological data.

Identification of Interspecific and Intraspecific Single Nucleotide Polymorphisms in Papaver spp.

( Seon-hwa Bae ),( Jae-hyeon Oh ),( Jundae Lee ) 한국육종학회 2021 Plant Breeding and Biotechnology Vol.9 No.1

The plants of the Papaveraceae family are used for ornamental purposes because of their varicolored flowers, and are known as medicinal crops. Some species of poppy are used in foods such as salads or sorbets, utilizing the seeds, leaves, pedicels, and petals. There are several morphological similarities among the species of this family, which make it difficult to distinguish the seeds of different species or identify opium poppies. The family is known to contain about 100 species. The leaves of Iceland poppy (Papaver nudicaule) cultivars with five different flower colors (white, yellow, pink, orange, and scarlet) were sequenced to obtain transcriptome data. Sequencing was done on plants in three different developmental growth stages (leaf rosette, branching and elongation of internodes, and blossom and seed formation). Systematic bioinformatics analysis was conducted to identify single nucleotide polymorphisms (SNPs) unique to the five Papaver nudicaule cultivars and two other Papaver species (Papaver rhoeas and Papaver somniferum). A 739-Mb reference transcriptome (94.6% BUSCO completeness score) from a 566-Gb RNA-sequencing (RNA-Seq) dataset was obtained. Likewise, 18 significant SNPs were identified to authenticate the three species and five cultivars of Papaver. This study will facilitate future Papaver research, including evaluation of the results for more detailed characterization.

A New Flavonol Glycoside from Hylomecon vernalis

Seung Young Lee,이강노,김기현,이일균,Kyu Ha Lee,Sang Un Choi 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.3

Purification of a MeOH extract from the aerial parts of Hylomecon vernalis Maxim. (Papaveraceae) using column chromatography furnished a new acetylated flavonol glycoside (1), together with twenty known phenolic compounds (2-21). Structural elucidation of 1 was based on 1D- and 2D-NMR spectroscopy data analysis to be quercetin 3-O-[4'''-O-acetyl-α-L-arabinopyranosyl]-(1'''→6'')-β-D-galactopyranoside (1). The structures of compounds 2-21 were elucidated by spectroscopy and confirmed by comparison with reported data; quercetin 3-O-[2'''-O-acetyl-α-L-arabinopyranosyl]-(1'''→6'')-β-D-galactopyranoside (2), quercetin 3-O-α-L-arabinopyranosyl-(1'''→6'')-β-D-galactopyranoside (3), quercetin 3-O-β-D-galactopyranoside (4), kaempferol 3,7-O-α-L-dirhamnopyranoside (5), diosmetin 7-O-β-D-glucopyranoside (6), diosmetin 7-O-β-D-xylopyranosyl-(1'''→6'')-β-D-glucopyranoside (7), p-hydroxybenzoic acid (8), protocatechuic acid (9), caffeic acid (10), 6-hydroxy-3,4-dihydro-1-oxo-β-carboline (11), (Z)-3-hexenyl-β-D-glucopyranoside (12), (E)-2-hexenyl-β-D-glucopyranoside (13), (Z)-3-hexenyl-α-Larabinopyranosyl-(1''→6')-β-D-glucopyranoside (14), oct-1-en-3-yl-α-L-arabinopyranosyl-(1''→6')-β-D-glucopyranoside (15), benzyl-β-D-apiofuranosyl-(1''→6')-β-D-glucopyranoside (16), benzyl-α-L-arabinopyranosyl-(1''→6')-β-D-glucopyranoside (17), benzyl-β-D-xylopyranosyl-(1''→6')-β-D-glucopyranoside (18), 2-phenylethyl-α-L-arabinopyranosyl-(1''→6')-β-D-glucopyranoside (19), 2-phenylethyl-β-D-apiofuranosyl-(1''→6')-β-D-glucopyranoside (20), and aryl-β-D-glucopyranoside (21). Compounds 2-21 were isolated for the first time from this plant. The isolated compounds were tested for cytotoxicity against four human tumor cell lines in vitro using a Sulforhodamin B bioassay.

들현호색으로부터 Berberine과 Coptisine의 분리 및 함량분석

이향이,김종원,Lee, Hyang-Yi,Kim, Chong-Won 한국생약학회 1999 생약학회지 Vol.30 No.3

Corydalis Tuber has been used in traditional medicine for an analgesic, antispasmodic and gastric ulcers. For the quality control on this drug, isolation and quantitative determination of berberine and coptisine from Corydalis ternata Nakai (Papaveraceae) has been conducted by using HPLC method. Berberine and coptisine in quarternary alkaloidal fraction from the crude drug were separated on silicagel column using a $CHCl_3:MeOH\;(85:15)$ and $CHCl_3:MeOH:H_2O\;(70:30:4)$ as an eluent, and the average contents were about 0.93 and 0.36%.

식물세포배양에 의한 Corydalis Alkaloid의 생산(I)

장정인,신승원,지형준,Chang, Jung-In,Shin, Seung-Won,Chi, Hyung-Joon 한국생약학회 1995 생약학회지 Vol.26 No.4

Corydalis remota Fish. ex Max. (Papaveraceae) is a well known medicinal plant being used as analgesics or anticonvulsive in oriental medicine. As the alkaloid content is known to vary depending on the environmental factors, the technology of plant tissue culture can be adopted as source of Corydalis-alkaloids. The present study describes an establishment of tissue cultures of Corydalis which produce alkaloids consistently. Callus were induced from immature seeds of Corydalis remota by placing the seeds on MS static media containing NAA(0.25, 1.0 and 4.0 mg/l, respectively). The combined treatment of NAA(1.0 mg/l) with cytokinin(BAP 0.5 mg/l) improved the induction of callus. TLC scanning data followed by sequential extraction and purification revealed that the induced callus contains a significant amount of alkaloids. Cell suspension cultures were established by transferring the induced callus into the liquid media with the same condition of plant growth regulators as the callus culture.

Cytotoxic Isoquinoline Alkaloids from the Aerial Parts of Corydalis incisa

Sang Un Choi,백남인,김성훈,Jae Heon Yang,은재순,신태용,임종필,이재혁,전훈,윤미영,임강현,박희욱,김대근 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.2

Three known isoquinoline alkaloids were isolated from the chloroform-soluble fraction of the methanolic extract of the aerial parts of Corydalis incisa (Papaveraceae) through repeated column chromatography. Their chemical structures were elucidated as corynoline (1), corynoloxine (2) and 6-oxocorynoline (3) using spectroscopic analysis. Compounds 1-3 exhibited cytotoxicity against human A549, SK-OV-3, SK-MEL-2 and HCT15 tumor cells.