목단피 추출물이 RAW264.7 대식세포주에서 유도 일산화질소 합성효소 유전자의 발현 억제 요구

손성용,설종구 大韓泌尿器科學會 2002 Korean Journal of Urology Vol.43 No.6

Inhibitory Effects of <i>Paeonia suffruticosa</i> on Allergic Reactions by Inhibiting the NF-kappaB/IkappaB-alpha Signaling Pathway and Phosphorylation of ERK in an Animal Model and Human Mast Cells

HONG, Myung Hee,KIM, Jeong-Hyun,NA, Sang Hyuk,BAE, Hyunsu,SHIN, Yong-Cheol,KIM, Sung-Hoon,KO, Seong-Gyu Japan Society for Bioscience, Biotechnology, and A 2010 Bioscience, biotechnology, and biochemistry Vol.74 No.6

<P>The root cortex of <I>Paeonia suffruticosa</I> Andrews (PSA), also known as Moutan Cortex, is known to have anti-allergic and anti-inflammatory properties. This study investigates the effect and mechanism of PSA by <I>in vivo</I> and <I>in vitro</I> methods. Treatings the root cortex from PSA with up to 0.4 mg/ml of an ethanol extract showed no cytotoxicity in human mast cells. The ethanol extract of PSA (200 mg/kg) significantly inhibited the passive cutaneous anaphylaxis reaction <I>in vivo</I>, and suppressed the release of histamine from rat peritoneal mast cells induced by compound 48/80. It was also found that PSA decreased the expressions of TNF-alpha and IL-6 in PMA- and A23187-stimulated HMC-1 cells. The results show the inactivation of IkappaB-alpha and NF-kappaB, as well as suppression of the phosphorylation of extracellular signal-regulated kinase (ERK). Our findings therefore suggest that PSA could be promising for anti-allergic inflammation by inhibiting the NF-kappaB/IkappaB-alpha signaling pathway and the phosphorylation of ERK.</P>

Ethanol extract of paeonia suffruticosa Andrews (PSE) induced AGS human gastric cancer cell apoptosis via fas-dependent apoptosis and MDM2-p53 pathways

Choi, Hyeong Sim,Seo, Hye-Sook,Kim, Ji Hye,Um, Jae-Young,Shin, Yong Cheol,Ko, Seong-Gyu BioMed Central 2012 JOURNAL OF BIOMEDICAL SCIENCE -BASEL- Vol.19 No.1

<P><B>Background</B></P><P>The root bark of <I>Paeonia suffruticosa</I> Andrews (PSE), also known as Moutan Cortex, has been widely used in Asia to treat various diseases. The molecular mechanisms by which PSE exerts its anti-oxidant and anti-inflammatory activities are well known, but its anti-cancer activity is not yet well understood. Here, we present evidence demonstrating that PSE can be used as a potent anti-cancer agent to treat gastric cancer.</P><P><B>Methods</B></P><P>The effects of the ethanol extract of PSE on cell proliferation were determined using an MTT (1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan) assay. Cell cytotoxicity induced by the PSE extact is measured using an LDH leakage assay. Flow cytometry was used to analyze the cell cycle and to measure the subG0/G1 apoptotic cell fraction. Apoptosis induced by the PSE extact is also examined using a DNA fragmentation assay. Western blot analysis is used to measure the levels of apoptotic proteins such as Fas receptor, caspase-8, caspase-3, PARP, Bax, Bcl-2, MDM2, and p53.</P><P><B>Results</B></P><P>This study demonstrated that treating AGS cells with the PSE extact significantly inhibited cell proliferation and induced cytotoxicity in a dose- and time-dependent manner. The PSE extract also induced apoptosis in AGS cells, as measured by flow cytometry and a DNA fragmentation assay. We found that the PSE extract induced apoptosis via the extrinsic Fas-mediated apoptosis pathway, which was concurrent with the activation of caspases, including caspase-8 and caspase-3, and cleavage of PARP. The MDM2-p53 pathway also played a role in the apoptosis of AGS cells that was induced by the PSE extract.</P><P><B>Conclusions</B></P><P>These results clearly demonstrate that the PSE extact displays growth-suppressive activity and induces apoptosis in AGS cells. Our data suggest that the PSE extact might be a potential anti-cancer agent for gastric cancer.</P>

Protective effects of a herbal extract combination of <i>Bupleurum falcatum</i>, <i>Paeonia suffruticosa</i>, and <i>Angelica dahurica</i> against MPTP-induced neurotoxicity via regulation of nuclear receptor-related 1 protein

Sim, Yeomoon,Park, Gunhyuk,Eo, Hyeyoon,Huh, Eugene,Gu, Pil Sung,Hong, Seon-Pyo,Pak, Youngmi Kim,Oh, Myung Sook Elsevier 2017 NEUROSCIENCE Vol.340 No.-

<P><B>Abstract</B></P> <P>Parkinson’s disease (PD) is one of the progressive neurodegenerative diseases of whose condition is characterized by dopaminergic neuronal cell loss and dysfunction in the substantia nigra pars compacta (SNpc) and the striatum. Recent studies have demonstrated that the nuclear receptor-related 1 protein (Nurr1) is critical of dopaminergic phenotype induction in mesencephalic dopaminergic neurons. Further, Nurr1 engages in synthesizing and storing dopamine through regulating levels of tyrosine hydroxylase (TH), dopamine transporter (DAT) and vesicular monoamine transporter 2 (VMAT2). The aim of this study was to investigate the protective effects of a herbal extract combination, consisting of <I>Bupleurum falcatum</I>, <I>Paeonia suffruticosa</I>, and <I>Angelica dahurica</I> (MABH), on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD-like symptoms and to elucidate possible mechanisms of action focusing on Nurr1. In a subacute mouse model of MPTP-induced PD, MABH treatment resulted in recovery from movement impairments. MABH prevented dopamine depletion and protected against dopaminergic neuronal degradation induced by MPTP. Additionally, MABH increased Nurr1 expression in the SNpc of mice. To evaluate the effects of MABH on Nurr1 expression, we measured the protein levels of Nurr1 and its regulating factors using Western blot analysis in PC12 cells. MABH treatment induced the phosphorylation of extracellular signal-regulated kinase protein via increasing the protein expression levels of Nurr1 and ultimately the levels of TH, VMAT2, and DAT. These results indicate that MABH has protective effects on dopaminergic neurons in a mouse model of PD by regulating Nurr1.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MABH improved MPTP-induced movement impairments in mice. </LI> <LI> MABH prevented dopamine depletion and protected against MPTP-induced dopaminergic neuron damage in mice. </LI> <LI> MABH increased Nurr1 expression in the SNpc of mice. </LI> <LI> MABH increased TH, DAT, and VMAT2 expressions by upregulating Nurr1 via phosphorylation of ERK in PC12 cells. </LI> </UL> </P>

CT-26 세포 암 유발 마우스에서 Gallic acid의 항암 효과

이정희(Lee, Jung-He),최화정(Choi, Hwa-Jung),김범호(Kim, Pom-Ho) 한국산학기술학회 2014 한국산학기술학회논문지 Vol.15 No.10

본 연구에서 목단피로부터 분리한 gallic acid(GA)의 항암 활성을 평가하였다. 목단피로부터 GA는 스펙트로 분석법 (ESI-MS, 1H-NMR, and 13C-NMR)에 의해 구조를 밝혔으며, 항암활성은 마우스에 결장암을 유도시킨 후 하루에 한번 gallic acid(GA; 20, 100 mg/kg p.o)을 14일 동안 투여 한 후 암세포 크기를 측정하였다. 또한 GA투여 후 체중변화, 급성독성, 간과 비장의 무게 변화와 간의 생화학적 지표를 측정하였다. 결과로써 GA 처리군의 경우 체중 변화 및 급성 독성 없이 항암제 처리군과 비슷하게 암의 크기가 유의적으로 감소하였다(p<0.05). 또한 암의 유도에 의해 증가된 간과 비장의 무게 및 GSH, ALT, AST는 GA 처리에 의해 유의적으로 감소하였으며, 암의 유도에 의해 감소된 GSH는 GA 투여에 의해 유의적으로 증가 하였다(p<0.05). 이상의 연구 결과를 통해 GA는 항암제 개발을 위한 후보군으로 활용 가능하리라 사료된다. This study examined the anti-cancer activity of gallic acid(GA) isolated from P. suffruticosa. was analyzed by ESI-MS, 1H-NMR, and 13C-NMR. The anti-cancer activity was evaluated by measuring the cancer size in CT-26 cancer-allograft mice treated with GA(100 ㎎/㎏ p.o) for 14 days. The change in body weight, acute toxicity, weight change of the liver and spleen and biomaker of the liver were evaluated in the mice after the GA treatment. As a result, the cancer size of the CT-26 cancer-allograft mice treated with GA decreased significantly compared to that of the cancer mice without significant changes in weight loss (p<0.05) and acute toxicity. The weight of the liver and spleen and ALT, AST and LPO levels increased by cancer were decreased significantly after the GA treatment, and the GSH levels decreased by cancer were increased significantly with the GA treatment (p<0.05). Therefore, GA could be an attractive lead for the development of anticancer agents.

목단피((牧丹皮).도인(桃仁) 배합(配合)이 항염증(抗炎症) 작용(作用)에 미치는 영향

김영일,이성준,허진,이태형,신동근,이재철,신용서,윤용갑,Kim, Young-Il,Lee, Sung-Jun,Huh, Jin,Lee, Tae-Hyung,Shin, Dong-Gean,Lee, Jae-Cheol,Shin, Yong-Seo,Yun, Young-Gab 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.1

Paeonia Suffruticosa and Prunus Persica have been used as oriental medicine for removal of fever, alleviation of pain, an anti-phlogistic effect and removal of extravasated blood. However, it has been never shown the effects of these herbal medicines on anti-inflammatory processes. This experiment was performed to show how these herbs could act as anti-inflammatory medicines at cellular level. Anti-inflammation effects of water extracts from Paeonia Suffruticosa and Prunus Persica as well as their mixture have been investigated, and the results were follows; 1) each extract slightly suppressed the expression and production of inflammatory mediators and enzymes such as NO, iNOS, IL-$1{\beta}$, and TNF-$\alpha$ in lipopolysaccharid(LPS)-stimulated RAW264.7 cells and mouse primary peritoneal macrophages in a dose-dependent manner. These suppressive effects, however, were synergistically increased by their mixture. 2) Each extract of Paeonia Suffruticosa and Prunus Persica insignificantly suppressed the activation and activity of NF-${\kappa}B$ in LPS-stimulated RAW264.7 cells, which controls the expression of inflammatory mediators such as NO, iNOS, IL-$1{\beta}$, and TNF-$\alpha$. However, extract mixture of Paeonia Suffruticosa and Prunus Persica suppressed effectively the activation and activity of NF-${\kappa}B$. 3) Each of Paeonia Suffruticosa and Prunus Persica induced translocation of NF-${\kappa}B$ to the nucleus from the cytosol and DNA-binding activity of nuclear NF-${\kappa}B$ in LPS-activated RAW264.7 cells. The extract mixture of Paeonia Suffruticosa and Prunus Persica showed more significant suppression of the NF-${\kappa}B$ translocation and its DNA-binding activity, as compared to those of the each extract. These results suggest that the extract mixture of Paeonia Suffruticosa and Prunus Persica may affect different control mechanisms for NF-${\kappa}B$ activation and the expression and production of NF-${\kappa}B$-dependent inflammatory mediators, indicating that this extract mixture may be useful for treatment of inflammatory diseases.

목단피 물 추출물의 UVB와 Hydrogen peroxide로 유도된 산화적 손상에 대한 사람각질형성세포 보호효과

서승희 ( Seung Hee Seo ),최미옥 ( Mee Ok Choi ) 한국미용학회 2016 한국미용학회지 Vol.22 No.3

Keratinocytes is an important role to protecting the skin from the external environment for a variety of stimuli to the skin, and the inner material and the water is the key to defend the skin from being lost out. However, it is still largely unknown about effective protectors against ultraviolet B (UVB) or oxidative stress in human keratinocyte, HaCaT cells. Therefore, the purpose of this study was in exploring the ingredients to prepare a Paeonia suffruticosa extracts, we were evaluated for the cytoprotective effect on the oxidative damage induced by UVB and Hydrogen peroxide(H2O2). P. suffruticosa extracts showed the large amount of polyphenol content and total flavonoid content. In addition, DPPH scavenging, total antioxidant capacity and superoxide scavenging activity also showed a superior effect by P. suffruticosa extracts more than the positive control. Furthermore, P. suffruticosa extracts also exhibited an effect of HaCaT cells protection significantly against the UVB or H2O2 toxicity. Equivalent to P. suffruticosa extracts showed the effect of significantly inhibiting the DNA segment and pro-caspase 9 degradation on the UVB or H2O2-induced HaCaT cells. Based on the result of this comprehensive P. suffruticosa extracts have excellent antioxidant effects, and it also have protected the keratinocytes against the UVB or H2O2 HaCaT cells toxicity. Therefore, these results suggest that P. suffruticosa extracts can be used the development potential as a cosmetic material. The further study is necessary accordingly to find a standardization of the active ingredient.

모란과 작약의 구별을 위한 엽록체 기반 InDel 마커의 개발

이미선,정희정,박소현,정 희,정진태,정진태,김문교,김문교,길진수,이재복,김세림,윤기훈,이이 한국약용작물학회 2022 한국약용작물학회지 Vol.30 No.6

Background: Paeonia suffruticosa Andrew and P. lactiflora Pallas are important medicinal plants that are difficult to differentiate due to their similar appearances. The development of molecular markers will aid in the discrimination of these two species. Methods and Results: We found several chloroplast genomic loci that were polymorphic between P. suffruticosa and P. lactiflora. Among them, the insertion/deletion (InDel) marker PsPl-InDel-12 clearly discriminated 25 P. suffruticosa and P. lactiflora samples collected in the Republic of Korea. We were able to discriminate the two species in material of mixed status. Conclusions: The marker developed in this study can be used to ensure P. suffruticosa and P. lactiflora quality and increase consumer confidence when purchasing medicinal products made from these species.

목단피(牧丹皮) Methyl Gallate 성분의 항염증효능에 대한 연구

박용기 ( Yong Ki Park ),민지영 ( Ji Young Min ),이제현 ( Je Hyun Lee ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4

Objectives: In this study, we investigated the effect of methyl gallate of Paeonia suffruticosa(Moutan Cortex Radicis) on inflammatory response in activated macrophages. Methods: RAW264.7 cells were incubated with different concentrations of methyl gallate of Paeonia suffruticosa for 30 min and then stimulated with or without LPS at indicated times. Cell toxicity was determined by MTT assay. The concentrations of nitric oxide (NO), prostaglandin E2 (PGE2) and inflammatory cytokines (TNF-α, IL-6) were measured in culture medium by Griess assay, enzyme-immuno assay, and ELISA, respectively. The expressions of iNOS, COX-2 and cytokine mRNA and protein were determined by RT-PCR and Western blot, respectively. The Iκ-Bα degradation in cytosol and NF-κB p65 translocation into nuclear of the cells were determined by Western blot. Results: Methyl gallate was significantly inhibited LPS-induced production of NO and PGE2 in RAW264.7 cells. Methyl gallate was also suppressed LPS-induced expression of iNOS and COX-2 mRNA and protein in the cells. Methyl gallate was inhibited LPS-induced production of TNF-α and IL-6 via suppression of their mRNA expressions. Methyl gallate blocked the NF-κB pathway in LPS-stimulated RAW264.7 cells. Conclusions: This study suggests that methyl gallate of Paeonia suffruticosa may have an anti-inflammatory property through suppressing inflammatory mediator production in activated macrophages.

Bioassay-guided Isolation of Novel Compound from Paeonia suffruticosa Andrews Roots as an IL-1β Inhibitor

Yun-Hyeok Choi,Jung Ho Choi,Hee-Jung Yoo,Ill Chan Noh,Jeong-Min Lee,Jae Won Park,최완수 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.5

The inhibition of Interleukin-1beta (IL-1β) is of substantial interest for the treatment of rheumatoid arthritis. Using an in vitro assay with RAW 264.7 cells, oxo-acetic acid 2-ethoxy-4-(3-hydroxy-2-oxopropyl) phenyl ester (1) was isolated from the roots of Paeonia suffruticosa Andrews as an inhibitor of IL-1β with an IC50 value of 56 μM. Compound 1 is a novel phenylesteric compound from P. suffruticosa Andrews. Compound 1 was shown to inhibit the production of pro-inflammatory cytokines in RAW 264.7 cells. Thus, a possible new action of novel compound is provided explaining the anti-rheumatoid arthritic properties of P. suffruticosa Andrews.