Regulation of CYP1A1 and Inflammatory Cytokine by NCOA7 Isoform 4 in Response to Dioxin Induced Airway Inflammation

( Sung Hwan Cho ),( Shin Young Park ),( Eun Jeong Lee ),( Yo Han Cho ),( Hyun Sun Park ),( Seok Ho Hong ),( Woo Jin Kim ) 대한결핵 및 호흡기학회 2015 Tuberculosis and Respiratory Diseases Vol.78 No.2

Background: Aryl hydrocarbon receptor (AhR), a ligand-dependent transcription factor, binds to a wide variety of synthetic and naturally occurring compounds. AhR is involved in the regulation of inflammatory response during acute and chronic respiratory diseases. We investigated whether nuclear receptor coactivator 7 (NCOA7) could regulate transcriptional levels of AhR target genes and inflammatory cytokines in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-treated human bronchial epithelial cells. This study was based on our previous study that NCOA7 was differentially expressed between normal and chronic obstructive pulmonary disease lung tissues. Methods: BEAS-2B and A549 cells grown under serum-free conditions were treated with or without TCDD (0.15 nM and 6.5 nM) for 24 hours after transfection of pCMV-NCOA7 isoform 4. Expression levels of cytochrome P4501A1 (CYP1A1), IL-6, and IL-8 were measured by quantitative real-time polymerase chain reaction. Results: The transcriptional activities of CYP1A1 and inflammatory cytokines were strongly induced by TCDD treatment in both BEAS-2B and A549 cell lines. The NCOA7 isoform 4 oppositely regulated the transcriptional activities of CYP1A1 and inflammatory cytokines between BEAS-2B and A549 cell lines. Conclusion: Our results suggest that NCOA7 could act as a regulator in the TCDD-AhR signaling pathway with dual roles in normal and abnormal physiological conditions.

Regulation of CYP1A1 and Inflammatory Cytokine by NCOA7 Isoform 4 in Response to Dioxin Induced Airway Inflammation

Cho, Sung-Hwan,Park, Shin Young,Lee, Eun Jeong,Cho, Yo Han,Park, Hyun Sun,Hong, Seok-Ho,Kim, Woo Jin The Korean Academy of Tuberculosis and Respiratory 2015 Tuberculosis and Respiratory Diseases Vol.78 No.2

Background: Aryl hydrocarbon receptor (AhR), a ligand-dependent transcription factor, binds to a wide variety of synthetic and naturally occurring compounds. AhR is involved in the regulation of inflammatory response during acute and chronic respiratory diseases. We investigated whether nuclear receptor coactivator 7 (NCOA7) could regulate transcriptional levels of AhR target genes and inflammatory cytokines in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-treated human bronchial epithelial cells. This study was based on our previous study that NCOA7 was differentially expressed between normal and chronic obstructive pulmonary disease lung tissues. Methods: BEAS-2B and A549 cells grown under serum-free conditions were treated with or without TCDD (0.15 nM and 6.5 nM) for 24 hours after transfection of pCMV-NCOA7 isoform 4. Expression levels of cytochrome P4501A1 (CYP1A1), IL-6, and IL-8 were measured by quantitative real-time polymerase chain reaction. Results: The transcriptional activities of CYP1A1 and inflammatory cytokines were strongly induced by TCDD treatment in both BEAS-2B and A549 cell lines. The NCOA7 isoform 4 oppositely regulated the transcriptional activities of CYP1A1 and inflammatory cytokines between BEAS-2B and A549 cell lines. Conclusion: Our results suggest that NCOA7 could act as a regulator in the TCDD-AhR signaling pathway with dual roles in normal and abnormal physiological conditions.

Regulation of CYP1A1 and Inflammatory Cytokine by NCOA7 Isoform 4 in Response to Dioxin Induced Airway Inflammation

조성환,박신영,이은정,조요한,박현선,홍석호,김우진 대한결핵및호흡기학회 2015 Tuberculosis and Respiratory Diseases Vol.78 No.2

Background: Aryl hydrocarbon receptor (AhR), a ligand-dependent transcription factor, binds to a wide variety of synthetic and naturally occurring compounds. AhR is involved in the regulation of inflammatory response during acute and chronic respiratory diseases. We investigated whether nuclear receptor coactivator 7 (NCOA7) could regulate transcriptional levels of AhR target genes and inflammatory cytokines in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-treated human bronchial epithelial cells. This study was based on our previous study that NCOA7 was differentially expressed between normal and chronic obstructive pulmonary disease lung tissues. Methods: BEAS-2B and A549 cells grown under serum-free conditions were treated with or without TCDD (0.15 nM and 6.5 nM) for 24 hours after transfection of pCMV-NCOA7 isoform 4. Expression levels of cytochrome P4501A1 (CYP1A1), IL-6, and IL-8 were measured by quantitative real-time polymerase chain reaction. Results: The transcriptional activities of CYP1A1 and inflammatory cytokines were strongly induced by TCDD treatment in both BEAS-2B and A549 cell lines. The NCOA7 isoform 4 oppositely regulated the transcriptional activities of CYP1A1 and inflammatory cytokines between BEAS-2B and A549 cell lines. Conclusion: Our results suggest that NCOA7 could act as a regulator in the TCDD-AhR signaling pathway with dual roles in normal and abnormal physiological conditions.

Characterization of steroid receptor coactivator in sea urchin, <i>Strongylocentrotus nudus</i>, and its involvement in embryonic development

Kim, Mi Ae,Kim, Gil Jung,Maeng, Sejung,Jin, Deuk-Hee,Sohn, Young Chang Elsevier 2011 Molecular and cellular endocrinology Vol.331 No.1

<P><B>Abstract</B></P><P>Ligand-bound nuclear receptors (NRs) recruit coactivators such as members of the p160 steroid receptor coactivator (SRC) family and cyclic AMP responsive element binding protein (CREB)-binding protein (CBP) to specific enhancer elements and activate target gene transcription. In the present study, we isolated a novel SRC from the sea urchin <I>Strongylocentrotus nudus</I> (SnSRC) by using the ligand-binding domain of retinoid X receptor as a bait in a yeast two-hybrid screening. The SnSRC and vertebrate SRCs are different in size but share the overall characteristic domains, such as NR interacting domain (NID), CBP-binding and glutamine-rich regions. <I>SnSRC</I> mRNA showed highest expression levels at the 32-cell, 64-cell and pluteus larval stages. Full-length SnSRC (1992 amino acids) interacted with several NRs, including sea urchin estrogen receptor-related receptor (ERR), human and masu salmon estrogen receptors (ERα), mouse ERRγ, rat glucocorticoid receptor α, and rat thyroid receptor β. The SnSRC possesses two functional NIDs, both of which are dependent on their core LxxLL motifs. Furthermore, preferential interacting domains for ERα in the SnSRC are located in the central LxxLL motifs, revealed by the truncation and mutagenesis studies. Strikingly, the SnSRC has a single transcription activation domain, which interacts with CBP, a transcriptional integrator. In addition, transient knockdown of the <I>SnSRC</I> gene in the sea urchin embryo using morpholino antisense RNA induced abnormal phenotypes at gastrulation stage such as the lack of primary invagitation and exogastrulation. These results suggest that the SnSRC is a new member of the SRC family and plays an important role during early embryonic development.</P>

육군부사관의 역사와 발전방향 연구

김경수 ( Kim Kyung Su ),이진헌 ( Lee Jin Hun ),신진 ( Shin Jin ) 미래군사학회 2021 한국군사학논총 Vol.10 No.2

부사관은 "군사력의 중추"로 정의될 수 있다. 부사관이 전투력을 발휘하고 수행하는데 주력하는 반면, 장교는 전투력을 관리한다는 점에서 기인한다. 대부분의 전투는 중대급 이하에서 발생해 적과 직접 대치한다. 따라서 대부분 장교들은 계획을 수립하고, 부사관들은 소규모 부대장으로서 현장에서 병사들을 이끌고 전투력을 발휘한다. 현재 대한민국이 북한과 휴전 중인 것처럼 한반도에는 전쟁 가능성이 항상 존재한다. 또한, 부사관은 북한뿐만 아니라 다른 나라와의 다양한 전투 상황에서 소규모 부대 전투 지도자로서 역할을 수행해야 한다. 다만 부사관들의 현실을 고려할 때 미흡한 요소가 적지 않다. 예컨대 부사관에 대한 인지도와 처우가 부사관에 비해 열악하고 교육 수준도 떨어진다. 따라서, 부사관의 이력과 실상을 조사하고 발전방향에 대한 개선방향을 제시해야 했다. Non-commissioned officer can be defined as "The backbone of military combat power". It is due to the fact that non-commissioned officers focus on displaying and performing the combat power, while commissioned officers manage the combat power. Most of the combats occur at the company level or lower echelon, confronting the enemy directly. Therefore, commissioned officers mostly establish the plans and non-commissioned officers show the combat power as leaders of small units by leading the soldiers at the field. As Currently, Republic of Korea at an armistice with North Korea, there is always the possibility of war on the Korean Peninsula. Also, non-commissioned officers have to perform their roles as small unit combat leaders in various combat situations with not only North Korea, but also other countries. However, there are many insufficient factors, considering the reality of non-commissioned officers. For example, the recognition and treatment of non-commissioned officers are worse than the commissioned officers and the level of education is also deficient. Thus, the history and reality of a non-commissioned officer had to be investigated and the direction of improvement had to be provided to development direction.

자동차 무선 네트워크 환경에서 연속적이고 견고한 IPv6기반의 이동성 프로토콜

오하영(Hayoung Oh),김종권(Chong-kwon Kim) 한국정보과학회 2010 정보과학회논문지 : 정보통신 Vol.37 No.2

자동차 관련 응용 프로그램에 대한 요구가 빠르게 증가되면서, 자동차 무선 네트워크에서 연속 적이고 견고한 멀티미디어 서비스를 위한 필요성이 대두되고 있다. 이동성이 있는 환경에서 연속적인 서비스를 제공해주기 위해, 기본적인 이동성 프로토콜인 MIPv6은 물론 MIPv6의 문제점을 해결하기 위한 FMIPv6와 같은 프로토콜들이 개발되었다. 하지만, MIPv6, FMIPv6는 긴 핸드오버 지연시간과 패킷 손실문제로 인해 멀티미디어와 같이 품질 보증이 필요한 서비스에는 사용할 수 없다. 따라서 본 논문은 자동차 무선 네트워크 환경에서 자동차가 처음 속해있던 액세스 라우터에서 만든 원래 임시 주소(oCoA : original CoA)와 뒷단 주소 중복성 검사(background DAD : Duplicate Address Detection)를 이용하여 연속적이고 견고한 멀티미디어 서비스를 제공해줄 수 있는 이동성 프로토콜을 제안한다. 성능평가를 통해 제안한 방식이 자동차 무선 네트워크 환경에서 제일 적합함을 증명한다. With the rapidly increasing demand of traffic applications, the need to support seamless and robust multimedia services in the Vehicular Ad hoc Network is growing. Fast handover for the MIPv6 (FMIPv6) developed to solve the MIPv6’s problem as well as Mobile IPv6 (MIPv6) for basic mobility protocol have been developed to support seamless handover in mobile environment. However, MIPv6 and FMIPv6 are useless for Quality-of-Service (QoS) services such as multimedia applications due to the long handover latency and the packet loss problem. In this paper, we propose a seamless and robust handover scheme that supports multimedia services in Vehicular Ad hoc Network using oCoA and background DAD. Through performance evaluation, we show that the proposed scheme is more proper for Vehicular Ad hoc Network than other schemes.