말라리아 환자의 간스캔에서 폐섭취군과 비섭취군의 혈액생화학적 지표 및 임상양상의 비교

안석진 ( Seok Jin Ahn ),박상준 ( Sang Jun Park ),김윤권 ( Yun Kwon Kim ),김영중 ( Young Jung Kim ),김소연 ( So Yon Kim ),조민구 ( Min Gu Cho ),양병윤 ( Byeong Yun Yang ),김종수 ( Jong Soo Kim ),정재혁 ( Jae Hyuk Jung ),김지훈 ( Ji 대한내과학회 2007 대한내과학회지 Vol.73 No.2

Background: Lung uptake during liver scanning has been considered as a passing phenomenon related to several diseases, and especially infectious diseases and malignancy. Some reports have shown diffuse lung uptake during liver scanning of malarial patients. Therefore, we tried to determine the relationship between the abnormalities of the clinical features, including the hematobiochemical indices and the lung uptake during liver scanning, by analyzing the information of the malarial patients. Methods: We performed 99mTechnethium(Tc)-sulfur colloid liver scanning on 20 of the 45 malarial patients who were admitted from 1999 to 2004. We divided them into two groups, the Lung-Uptake (LU) group and the Non-Lung-Uptake (NLU) group. We analyzed the hematobiochemical indices and clinical features, including the respiratory symptoms, between the two groups. Results: 10 of the 20 malarial patients showed lung uptake on the liver scan. The mean platelet counts were 74,000/L and 165,000/L, respectively, in the LU group and the NLU group (p=0.012). Also, the mean total cholesterol levels were 80.3 mg/dL and 105.7 mg/dL, respectively, in the LU group and the NLU group (p=0.033). The scores of the bone marrow (BM) uptake in the LU group were higher than those in the NLU group (p=0.008). Yet the other values such as Hb, ALT, albumin and total bilirubin were not statistically significant, nor were the peak body temperatureand other features. Conclusions: Half of the patients had lung uptake on the liver scanning, and this may be considered as a characteristic of vivax malaria. The BM uptake during liver scanning in the LU group was more increased, and this is supposed to be a consequence of hyperstimulated reticuloendothelial system, which was accompanied by thrombocytopenia and a lower level of total cholesterol in malarial patients.(Korean J Med 73:183-191, 2007)

Destroying Deep Lung Tumor Tissue through Lung-Selective Accumulation and by Activation of Caveolin Uptake Channels Using a Specific Width of Carbon Nanodrug

Kim, Sang-Woo,Park, Jun-Young,Lee, Soyoung,Kim, Sang-Hyun,Khang, Dongwoo American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.5

<P>The main difficulty With current anticancer nanotherapeutics comes from the low efficiency of tumor targeting. Althoughniany strategies have been investigated, including cancer-specific antibody conjugation, lung tumors remain one of the invulnerable types of cancer that must be overcome in the near future. Meanwhile, despite their advantageous physiochemical properties, carbon nanotube structures are not considered safe medical drug delivery agents, but are considered a hazardous source that may cause pulmonary toxicity. However, high-aspect-ratio (width vs. length) nanostructures can he used as very efficient drug delivery agents due to their lung tissue accumulation property. Furthermore, selection of a specific width if the carbon nanostructures can activate additional caveolin uptake channels in cancer cells, thereby maximizing internalization of the nanodrug. The present study aimed to evaluate the therapeutic potential of carbon nanotube-based nanodrugs having various widths (10-30 nm, 60-100 mn, and 125-150 nm) as a delivery agent to treat lung tumors. The results of-the present study provided evidence that both lung tissue accumulation (passive targeting) and caveolin-assisted uptake (active targeting) can simultaneously contribute to the destruction of lung tumor tissues of carbon nanotube.</P>

중추신경계 질환에서 전신 스캔으로 본 Tc-99m ECD의 생체 내 분포

류종걸,전석길,김순 啓明大學校 醫科大學 1999 계명의대학술지 Vol.18 No.1

각종 중추신경계 질환에서 Tc-99m ECD를 이용하여 국소뇌혈류 SPECT를 시행할 때, 생체 내 다른 부위에 어떻게 분포되는가를 알아보고자 하였다. 중추신경계 질환으로 국소뇌혈류 SPECT를 시행한 150례에서 800 MBq의 Tc-99m ECD를 정맥 주사한 후 1시간에 전신 스캔 하였다. 대상 환자는 현훈(vertigo, 64명), 간질(seizure, 30명), 정신과적 증상(psychiatric symptoms, 9명), 외상후증후군(pose-traumatic syndrome, 7명), 뇌혈관사고(cerebrovascular accident, 34명), 기타 (6명) 등 6개의 임상군으로 구분하였다. 전신 스캔 영상에서 뇌(B), 간(H), 및 폐(P)에 동일한 크기의 관심영역을 그려 mean counts/pixel을 구한 후, H/B 및 P/B 비율을 분석하였다. Tc-99m HMPAO 국소뇌혈류 SPECT를 시행한 12명에서 동일한 연구를 시행하여 Tc-99m ECD로 얻은 결과와 비교하였다. 전체 대상환자에서 Tc-99m ECD의 평균 H/B 및 평균 P/B 비율은 각각 0.99±0.35, 0.50±0.26이었다. 평균 H/B 및 평균 P/B 비율은 연령군 사이에서 유의한 차이를 보이지 않았으나, 남자의 평균 H/B 및 평균 P/B 비율(1.11±0.30, 0.57±0.29)은 여자(0.86±0.35, 0.42±0.20)보다 유의하게 높았다.(p<0.05). 임상군 사이에 평균 H/B 비율은 유의한 차이가 없었으나, 뇌혈관사고의 평균 P/B 비율은 다른 임상군보다 높았다.(p<0.05), Tc-99m ECD의 평균 H/B 및 평균 P/B 비율(0.99±0.35, 0.50±0.26)은 Tc-99m HMPAO (2.40±0.63, 1.09±0.34)보다 훨씬 낮았다.(p<0.05). Tc-99m ECD의 간 섭취는 뇌 섭취와 거의 같은 정도였으며 폐 섭취는 뇌 섭취보다 낮아, 정상인에서의 결과와 유사하였다. Tc-99m ECD의 간과 폐에서의 상대적 섭취율은 여자보다 남자에서 높았으며, 폐에서의 상대적 섭취율은 다른 임상군보다 뇌혈관사고에서 높았다. Tc-99m ECD는 간과 폐에서의 상대적 섭취율이 Tc-99m HMPAO 보다 낮아 표적장기인 뇌에서의 섭취가 상대적으로 높았다. We evaluated prospectively in vivo distribution of Tc-99m Ethyl cysteinate dimer (ECD) in patients with central nervous system (CNS) diseases, especially in brain, liver and lung. Whole body scans were performed in 150 patients with CNS diseases at one hour after the injection of 800 MBq Tc-99m ECD. The clinical comments were divided into six groups such as vertigo (n=64), seizure (n=30), psychiatric symptoms (n=9), post-traumatic symptoms (n=7), cerebrovascular accident (CVA, n=34) and others (n=6). Mean counts/pixel of brain (B), liver (H), lung (P) were calculated with same-sized region of interest (ROI)s in whole body scan, and H/B and P/B ratios were analyzed. The same kind of study with Tc-99m Hexamethylpropylene amine oxime (HMPAO) was done in twelve cases. Average H/B and P/B ratios in all patients studied with Tc-99m ECD were 0.99±0.35 and 0.50±0.26, respectively. Average H/B and P/B ratios in male (1.11±0.30 and 0.57±0.29) were significantly higher than those in female (0.86±0.35 and 0.42±0.20) (p < 0.05). The H/B ratio was not significantly different among six different clinical comment groups, but P/B ratio in CVA was significantly higher than those in other groups (p < 0.05). Average H/B and P/B ratios of Tc-99m ECD (0.99±0.35 and 0.50±0.26) were much lower than those of Tc-99m HMPAO (2.40±0.63 and 1.09±0.34)(p<0.05). The hepatic uptake of Tc-99m ECD was nearly same as the brain uptake, and lung uptake was lower than that of brain, similar to those in normal volunteers. Liver and lung uptakes of Tc-99m ECD in male were higher than those in female, and lung uptake in CVA was higher than those in other clinical comment groups. Tc-99m ECD was more safe than Tc-99m HMPAO in view of the radiation exposure to liver and lung.

일상적인 운동 부하 게이트 심근 관류 SPECT에서 90mTc-sestsmibi 폐섭취 : 좌심실 구혈률과 관류 결손 정도와의 비교 Comparison with Left Ventricular Ejection Fraction and Severity of Perfusion Defect

정신영,이재태,배진호,안병철,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.2

배경 및 목적 : ^201TI 심근 관류 SPECT에서, 폐-심장 섭취비(lung-to-heart uptake ratio; LHR)의 정량적 측정은 관동맥 질환자의 예후를 평가할 수 있는 지표이다. 그러나 최근 ^99mTc-sestamibi (MIBI)를 이용한 심근 관류 SPECT가 많이 이용되고 있으나 MIBI의 폐섭취가 관동맥 질환자에 ^201TI LHR와 같은 의미를 가지는 지는 의문이 있어왔다. 본 연구는 MIBI 주사후 1시간에 촬영한 전면상을 이용하여 LHR을 구하여 좌심실 구혈률, 관류 결손 정도와의 상관 관계를 알아보고자 하였다. 대상 및 방법 : Bruce 법으로 운동 부하를 실시한 241명(남성 130명, 평균 연령 55세)을 대상으로 하였다. 부하검사와 휴식기 검사를 같은 날에 실시하는 프로토콜(1일 영상법)은 152명, 다른 날에 실시하는 프로토콜(2일 영상법)은 89명에서 시행하였다. 운동 부하 SPECT는 MIBI를 주사한 후 1시간에 이중헤드 감마 카메라를 이용하여 얻었다. 폐와 심장의 MIBI 섭취 정도는 20초간 얻은 전면상에서 폐와 심장에 관심영역을 설정하여 구하고, 이를 이용하여 섭취비를 계산하였다. AutoQUANT 소프트 웨어를 이용한 자동화 방법으로 운동부하시 좌심실 구혈렬(LVEF)을 구하였고 운동부하시 관류 결손 정도(Summed Stress Score, SSS)를 구하였다. LVEF에 따라 3군(35%이하, 36-50%, 50%초과), SSS에 따라 4군(4미만, 4-8, 9-13, 13초과)으로 나누었다. 증가된 LHR의 정의는 관류 결손이 없는 남, 여 각 군에서 구한 LHR의 평균값에 표준편차의 2배값을 더한 값을 초과하는 것으로 판단하였고 각 군에서 증가된 LHR을 보인 환자의 분포를 구하여 비교하였다. 결과 : 정상 관류 소견을 보였던 30명의 분석에서, 여성에 비해 남성 에서 LHR이 유의하게 높았고 정상 관류 소견을 보였던 130명의 남성과 여성 모두에서, 1일 영상법와 2일 영상법을 실시한 군 사이의 LHR은 의미 있는 차이가 관찰되지 않았다. 정상 관류 소견을 보인 군과 관류 결손을 보인 군 사이의 비교에서는 관류 결손이 있는 남성과 여성에게 관류 결손이 없는 남성과 여성에 비해 LHR이 유의하게 높았다. 관류 결손이 있는 111명의 분석에서는 LHR과 EF는 역상관 관계, LHR과 SSS 사이에는 야의 상관 관계가 관찰되었고, 남성에서는 통계학적 의의가 있었으나 여성에서는 통계학적 의의는 없었다. EF군에 따른 분석에서는 좌심실 구혈률이 낮을수록 LHR이 높았고, SSS군에 따른 분석에서 관류 결손이 높은 군에서 유의하게 LHR가 높았다. 결론 : 일상적인 운동부하 심근 관류 SPECT상 관류 결손이 있는 환자에서 폐-심장 섭취비는 좌심실 구혈률과 심근 부하시 관류 결손 정도가 클수록 증가된 폐-심장 섭취비를 보인 환자가 많았으며, 좌심실 구혈률이 낮을수록 그리고 관류 결손의 정도가 클수록 폐-심장 섭취비가 증가하였다. 이런 결과로 보아 주사후 1시간 ^99mTc-MIBI의 LHR은 좌심실 부전과 큰 관류 결손을 나타내는 간접적인 지표로 생각되어 진다. 그러나 관류 결손의 정도나 좌심실 구혈률 감소 정도가 미약한 군에서는 폐-심장 섭취비가 정상군과 비슷하여, 저위험군의 감별에는 제한점이 있다. Background: Lung-to-heart uptake ratio (LHR) in ^201TI-chloride myocardial perfusion scan is believed to be a reliable marker for left ventricular (LV) dysfunction, but the clinical value of LHR is controversial for ^99mTc-MIBI imaging. Furthermore, most of results suggesting lung uptake of ^99mTc-MIBI as a potential marker for LV dysfunction used immediate post-stress images, instead of routine images acquired 1 hour after tracer injection. The goal of our study was to investigate whether LHR evaluated with routine gated ^99mTc-MIBI imaging can reflect the degree or perfusion defect or left ventricular performance. Subjects and Methods: 241 patients underwent exercise ^99mTc-MIBI myocardial SPECT were classified into normal myocardial perfusion (NP, n=135) and abnormal myocardial perfusion (AP, n=106) group according to the presence of perfusion defect LHR was calculated from anterior projection image taken at 1-hour after injection. Two regions of interest (ROIs) were placed on left lung above LV and on myocardium showing the highest radioactivity. Subjects were classified by left ventricular ejection fraction (LVEF), as Gr-Ⅰ: >50%, Gr-Ⅱ: 36-50%, Gr-Ⅲ: <36% and by summed stress score (SSS), as Gr-A: <4, Gr-B:4-8, Gr-C: 9-13, Gr-D: >13, LHR was compared among these groups. Results: In NP group(n=135), LHR, were higher in men than women (men:0.311±0.03, women: 0.296±0.03, p<0.05). Significant difference, in LHR were found between NP and AP groups both for men and women (men: 0.311± 0.03 vs. 0.331± 0.06, women: 0.296±0.03 vs. 0.321±0.07, p<0.05). There were weak negative correlation between LFR and LVEF (r=-0.342, p<0.05) and weak positive correlation between LHR and SSS (r=0.478, p<0.05) in men, but not in women (LVEF: r=0.279, p=0.100, SSS: r=0.276, p=0.103). Increased LHR was defined when for more than mean + 2SD value (men≥0.38, women≥ 0.37) of the LHR of the subject with normal perfusion. Increased LHR were observed more frequently in subjects with lower LVEF (Gr-Ⅰ: 11.1%, Gr-Ⅱ: 27.0%, Gr-Ⅲ: 36.4%, p<0.05) and higher SSS(Gr-A: 14.0%, Gr-B: 6.7%m Gr-C: 18.2%, Gr-D: 40.7%, p<0.05). Conclusions: LHRs obtained from routine ^99mTc-MIBI gated SPECT images were weakly correlated with LVEF and perfusion defect. Although significant overlaps were observed between normal and abnormal perfusion group, LHRs could be used as an indirect marker of severe perfusion defect or reduced left ventricular function.

관상동맥질환에서 디피리다몰 부하 99mTc - MIBI 심근스캔의 폐 / 심장 섭취율

고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),서정돈(Jung Don Seo),이동수(Dong Soo Lee),최창운(Chang Woon Choi),이경한(Kyung Han Lee),강건욱(Keon Wook Kang) 대한핵의학회 1993 핵의학 분자영상 Vol.27 No.2

N/A Lung/heart uptake ratio (L/H R) in 201T1 myocardial perfusion scan is a reliable marker for long-term prognosis in patients with coronary artery disease. However, the value of L/H R in Tc-99m-MIBI myocardial perfusion scan is controversial in determining the prognosis and severity of the coronary artery disease. The purpose of this study was to determine the clinical implications of L/HR in Tc-99m-MIBI myocardial perfusion scan. Forty five patients who received Tc-99m-MIBI myocardial perfusion scan were divided into control group and coronary artery disease (CAD) group by their clinical findings, EKGs, and Tc-99m-MIBI myocardial perfusion scans. Twenty five patients in CAD group were, divided into ischemicc group and infarct group according to their results from Tc-99m-MIBI myocardial perfusion scan. L/H R was calculated on the anterior planar view, 60 minutes after infusion of dipyridamole. Two regions of interest (ROI) were placed on the left lung area 8 pixel above the left ventricle and on the myocardial area which had the highest radioactivity. In the control group, there were no significant differences of L/H R according to sex and age. No significant difference of L/H R was found between the control and CAD group (0.26±0.06, 0.29±0.05, p>0.05). In the CAD group, there was also no significant difference of L/H R between the ischemic group and infarct group (0.29±0.07, 0.30±0.04, p〉0.05). L/H R in CAD group did not show correlations with the defect are of stress polar map (r=0.18, p〉0.05) and with the sum of severity weighted extent score or reversibility score which represent severity and extent of myocardial perfusion defect area in stress (r=0.18, p〉0.05). We conclude that it is difficult to use L/H R as a marker for severity of CAD in dipyridamole Tc-99m-MIBI myocardial perfusion scan.

Maximum standardized uptake value at pre-treatment PET in estimating lung cancer progression after stereotactic body radiotherapy

박지선,최윤선,안기정,박성광,조흥래,이지영 대한방사선종양학회 2019 Radiation Oncology Journal Vol.37 No.1

Purpose: This study aimed to identify the feasibility of the maximum standardized uptake value (SUVmax) on baseline 18F-fluorodeoxyglucose positron emission tomography-computed tomography (FDG PET/CT) as a predictive factor for prognosis in early stage primary lung cancer treated with stereotactic body radiotherapy (SBRT). Materials and Methods: Twenty-seven T1-3N0M0 primary lung cancer patients treated with curative SBRT between 2010 and 2018 were retrospectively evaluated. Four patients (14.8%) treated with SBRT to address residual tumor after wedge resection and one patient (3.7%) with local recurrence after resection were included. The SUVmax at baseline PET/CT was assessed to determine its relationship with prognosis after SBRT. Patients were divided into two groups based on maximum SUVmax on pre-treatment FDG PET/ CT, estimated by receiver operating characteristic curve. Results: The median follow-up period was 17.7 months (range, 2.3 to 60.0 months). The actuarial 2-year local control, progressionfree survival (PFS), and overall survival were 80.4%, 66.0%, and 78.2%, respectively. With regard to failure patterns, 5 patients exhibited local failure (in-field failure, 18.5%), 1 (3.7%) experienced regional nodal relapse, and other 2 (7.4%) developed distant failure. SUVmax was significantly correlated with progression (p = 0.08, optimal cut-off point SUVmax > 5.1). PFS was significantly influenced by pretreatment SUVmax (SUVmax > 5.1 vs. SUVmax ≤ 5.1; p = 0.012) and T stage (T1 vs. T2-3; p = 0.012). Conclusion: SUVmax at pre-treatment FDG PET/CT demonstrated a predictive value for PFS after SBRT for lung cancer.

Relationship between 18F-FDG PET/CT Semi-Quantitative Parameters and International Association for the Study of Lung Cancer, American Thoracic Society/European Respiratory Society Classification in Lung Adenocarcinomas

Bu Lihong,Tu Ning,Wang Ke,Zhou Ying,Xie Xinli,Han Xingmin,Lin Huiqin,Feng Hongyan 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.1

Objective: To investigate the relationship between 18F-FDG PET/CT semi-quantitative parameters and the International Association for the Study of Lung Cancer, American Thoracic Society/European Respiratory Society (IASLC/ATS/ERS) histopathologic classification, including histological subtypes, proliferation activity, and somatic mutations. Materials and Methods: This retrospective study included 419 patients (150 males, 269 females; median age, 59.0 years; age range, 23.0–84.0 years) who had undergone surgical removal of stage IA–IIIA lung adenocarcinoma and had preoperative PET/CT data of lung tumors. The maximum standardized uptake values (SUVmax), background-subtracted volume (BSV), and background-subtracted lesion activity (BSL) derived from PET/CT were measured. The IASLC/ATS/ERS subtypes, Ki67 score, and epidermal growth factor/anaplastic lymphoma kinase (EGFR/ALK) mutation status were evaluated. The PET/CT semiquantitative parameters were compared between the tumor subtypes using the Mann–Whitney U test or the Kruskal–Wallis test. The optimum cutoff values of the PET/CT semi-quantitative parameters for distinguishing the IASLC/ATS/ERS subtypes were calculated using receiver operating characteristic curve analysis. The correlation between the PET/CT semi-quantitative parameters and pathological parameters was analyzed using Spearman’s correlation. Statistical significance was set at p < 0.05. Results: SUVmax, BSV, and BSL values were significantly higher in invasive adenocarcinoma (IA) than in minimally IA (MIA), and the values were higher in MIA than in adenocarcinoma in situ (AIS) (all p < 0.05). Remarkably, an SUVmax of 0.90 and a BSL of 3.62 were shown to be the optimal cutoff values for differentiating MIA from AIS, manifesting as pure ground-glass nodules with 100% sensitivity and specificity. Metabolic-volumetric parameters (BSV and BSL) were better potential independent factors than metabolic parameters (SUVmax) in differentiating growth patterns. SUVmax and BSL, rather than BSV, were strongly or moderately correlated with Ki67 in most subtypes, except for the micropapillary and solid predominant groups. PET/CT parameters were not correlated with EGFR/ALK mutation status. Conclusion: As noninvasive surrogates, preoperative PET/CT semi-quantitative parameters could imply IASLC/ATS/ERS subtypes and Ki67 index and thus may contribute to improved management of precise surgery and postoperative adjuvant therapy.

Maximum standardized uptake value at pre-treatment PET in estimating lung cancer progression after stereotactic body radiotherapy

Park, Jisun,Choi, Yunseon,Ahn, Ki Jung,Park, Sung Kwang,Cho, Heunglae,Lee, Ji Young The Korean Society for Radiation Oncology 2019 Radiation Oncology Journal Vol.37 No.1

Purpose: This study aimed to identify the feasibility of the maximum standardized uptake value (SUVmax) on baseline 18F-fluorodeoxyglucose positron emission tomography-computed tomography (FDG PET/CT) as a predictive factor for prognosis in early stage primary lung cancer treated with stereotactic body radiotherapy (SBRT). Materials and Methods: Twenty-seven T1-3N0M0 primary lung cancer patients treated with curative SBRT between 2010 and 2018 were retrospectively evaluated. Four patients (14.8%) treated with SBRT to address residual tumor after wedge resection and one patient (3.7%) with local recurrence after resection were included. The SUVmax at baseline PET/CT was assessed to determine its relationship with prognosis after SBRT. Patients were divided into two groups based on maximum SUVmax on pre-treatment FDG PET/CT, estimated by receiver operating characteristic curve. Results: The median follow-up period was 17.7 months (range, 2.3 to 60.0 months). The actuarial 2-year local control, progression-free survival (PFS), and overall survival were 80.4%, 66.0%, and 78.2%, respectively. With regard to failure patterns, 5 patients exhibited local failure (in-field failure, 18.5%), 1 (3.7%) experienced regional nodal relapse, and other 2 (7.4%) developed distant failure. SUVmax was significantly correlated with progression (p = 0.08, optimal cut-off point SUVmax > 5.1). PFS was significantly influenced by pretreatment SUVmax (SUVmax > 5.1 vs. SUVmax ≤ 5.1; p = 0.012) and T stage (T1 vs. T2-3; p = 0.012). Conclusion: SUVmax at pre-treatment FDG PET/CT demonstrated a predictive value for PFS after SBRT for lung cancer.

Pleural Carcinoembryonic Antigen and Maximum Standardized Uptake Value as Predictive Indicators of Visceral Pleural Invasion in Clinical T1N0M0 Lung Adenocarcinoma

Na Hye Rim,문석환,김경수,문미형,현관용,윤승근 대한심장혈관흉부외과학회 2024 Journal of Chest Surgery (J Chest Surg) Vol.57 No.1

Background: Visceral pleural invasion (VPI) is a poor prognostic factor that contributes to the upstaging of early lung cancers. However, the preoperative assessment of VPI presents challenges. This study was conducted to examine intraoperative pleural carcinoembryonic antigen (pCEA) level and maximum standardized uptake value (SUVmax) as predictive markers of VPI in patients with clinical T1N0M0 lung adenocarcinoma. Methods: A retrospective review was conducted of the medical records of 613 patients who underwent intraoperative pCEA sampling and lung resection for non-small cell lung cancer. Of these, 390 individuals with clinical stage I adenocarcinoma and tumors ≤30 mm were included. Based on computed tomography findings, these patients were divided into pleural contact (n=186) and non-pleural contact (n=204) groups. A receiver operating characteristic (ROC) curve was constructed to analyze the association between pCEA and SUVmax in relation to VPI. Additionally, logistic regression analysis was performed to evaluate risk factors for VPI in each group. Results: ROC curve analysis revealed that pCEA level greater than 2.565 ng/mL (area under the curve [AUC]=0.751) and SUVmax above 4.25 (AUC=0.801) were highly predictive of VPI in patients exhibiting pleural contact. Based on multivariable analysis, pCEA (odds ratio [OR], 3.00; 95% confidence interval [CI], 1.14–7.87; p=0.026) and SUVmax (OR, 5.25; 95% CI, 1.90–14.50; p=0.001) were significant risk factors for VPI in the pleural contact group. Conclusion: In patients with clinical stage I lung adenocarcinoma exhibiting pleural contact, pCEA and SUVmax are potential predictive indicators of VPI. These markers may be helpful in planning for lung cancer surgery.

Modulation of serum albumin protein corona for exploring cellular behaviors of fattigation-platform nanoparticles

Nguyen, Van Hong,Meghani, Nilesh M.,Amin, Hardik H.,Tran, Thao T.D.,Tran, Phuong H.L.,Park, Chulhun,Lee, Beom-Jin Elsevier 2018 Colloids and Surfaces B Vol.170 No.-

<P><B>Abstract</B></P> <P>Albumin is the most abundant protein in blood, and is the most frequently identified protein in the protein corona of nanoparticles (NPs). Thus, albumin plays an important role in modulating NPs’ physicochemical properties and bioavailability. In this study, the effect of bovine serum albumin (BSA) on gelatin-oleic nanoparticles’ (GONs) physicochemical properties and cellular uptake were evaluated. Coumarin-6 was used as indicator to track the cellular uptake of GONs. The binding of BSA onto the GON surface increased the size, slightly reduced the negative net charge of the GON, and improved GON stability. The presence of BSA in cell culture media reduced the cellular uptake of BSA-uncoated GONs on human embryonic kidney cells 293 (HEK 293) and human adenocarcinoma alveolar basal epithelial cells (A549) in the media without FBS addition. Pre-coated BSA corona decreased cellular uptake of GONs in A549 cells in the media, with and without supplemented with 10% fetal bovine serum (FBS) but drastically increased cellular uptake on HEK 293 cells. BSA could be used to modulate protein corona as an endogenous ligand in NP design simply by mixing or incubating BSA with NPs before in vivo administration to inhibit or induce cellular uptake in specific cell types.</P> <P><B>Highlights</B></P> <P> <UL> <LI> BSA adsorption increase GON size, reduced GON zeta potential and stabilized GONs. </LI> <LI> A lower cellular uptake of intact GONs was observed at a higher BSA concentration. </LI> <LI> BSA pre-coated GON modified cellular uptake of GONs in complete media. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>