방사선·알칼리 전처리 볏짚 당화액 및 당밀 활용Desmodesmus sp. KAERI-NJ5의 지질 생산성 비교 평가

조민호,김동호,배석 (사)한국방사선산업학회 2019 방사선산업학회지 Vol.13 No.3

Depending on species, microalgae can produce fatty acids more efficiently byusing organic carbon sources than by using the phototrophic cultivation mode. In this study,Desmodesmus sp. KAERI-NJ5 was grown in heterotrophic (HT) and mixotrophic (MT) modesusing enzymatic hydrolysate of rice straw pretreated with radiation and alkali solution to enhancebiodiesel feedstock productivity. In addition, biomass and lipid productivities were comparedwith the results from molasses based medium. Firstly, carbon source preference of the microalgawas determined. Although the presence of light caused a slight difference in the carbon sourcepreference, glucose was the most preferred carbon source in both types of cultivation. The ricestraw hydrolysate (RSH) was used as a carbon source and successfully utilized by the microalga. The biomass and lipid yield was higher in the HT cultivation mode than those from the MTcultivation mode. The final biomass and lipid yield of 6.0 and 3.4 g·l-1 was achieved, respectively,in HT cultivation. In addition, the conversion yield of consumed sugars to lipid and fatty acidmethyl esters (FAMEs) were 34.2 and 31.4%, respectively, which is even higher than that grownwith corresponding pure sugars. Analysis of the FAMEs composition revealed that palmitic, oleic,and stearic acids were the main FAMEs, and the FAMEs content was 52.19% in the HT cultivationmode corresponding to a 1.51-fold higher content than with MT cultivation. In addition, 92.52%of FAMEs consisted of saturated and monounsaturated fatty acids indicating good qualitybiodiesel feedstock. In addition, the productivities of biomass, total lipid and FAMEs were also evaluated when the microalga was grown with molasses, a representative low-cost carbon source,in both cultivation modes. The highest total lipid yield was 3.9 g·l-1 in the HT cultivation modecorresponding to 1.15-fold higher than that of the RSH grown microalga. However, the lipidproductivity was negligible considering the quality as a biodiesel feedstock, regional availabilityand sustainability of carbon sources. Thus, the RSH could be used as a carbon source for biodieselfeedstock production by Desmodesmus sp. KAERI-NJ5.

Optimization of Phototrophic Growth and Lipid Production of a Newly Isolated Microalga, Desmodesmus sp. KAERI-NJ5

( Min-ho Joe ),( Dong-ho Kim ),( Dae Seong Choi ),( Suk Bai ) 한국미생물 · 생명공학회 2018 한국미생물·생명공학회지 Vol.46 No.4

In this study, a novel microalgal strain, Desmodesmus sp. KAERI-NJ5, was isolated, identified, and evaluated as a candidate for biodiesel feedstock. In a preliminary study, the effects of four general microalgal growth factors, including temperature, pH, light intensity, and concentration of nitrogen source (KNO3), on the microalgal photoautotrophic growth were evaluated. With the exception of light intensity, the growth factors needed to be optimized for the microalgal biomass production. Optimization was done using response surface methodology. The optimal conditions for biomass production were pH 6.54, 27.66℃, and 0.52 g/l KNO3. The biomass production at the optimal conditions was 1.55 g/l, which correlated well with the predicted value of 1.5 g/l. The total lipid and fatty acid methyl ester contents of the cells grown at the optimal conditions were 49% and 21.2% of cell dry weight, respectively. To increase the lipid content of the biomass, microalgae were challenged by nitrogen starvation. Enhancement of total lipid and fatty acid content up to 52.02% and 49%, respectively, were observed. Lipid analysis of the nitrogen-starved cells revealed that C16 and C18 species accounted for 95.9% of the total fatty acids. Among them, palmitic acid (46.17%) and oleic acid (39.43%) dominantly constituted the algal fatty acids. These results suggest Desmodesmus sp. KAERI-NJ5 as a promising feedstock for biodiesel production.

Optimization of Phototrophic Growth and Lipid Production of a Newly Isolated Microalga, Desmodesmus sp. KAERI-NJ5

Joe, Min-Ho,Kim, Dong-Ho,Choi, Dae Seong,Bai, Suk The Korean Society for Microbiology and Biotechnol 2018 한국미생물·생명공학회지 Vol.46 No.4

In this study, a novel microalgal strain, Desmodesmus sp. KAERI-NJ5, was isolated, identified, and evaluated as a candidate for biodiesel feedstock. In a preliminary study, the effects of four general microalgal growth factors, including temperature, pH, light intensity, and concentration of nitrogen source ($KNO_3$), on the microalgal photoautotrophic growth were evaluated. With the exception of light intensity, the growth factors needed to be optimized for the microalgal biomass production. Optimization was done using response surface methodology. The optimal conditions for biomass production were pH 6.54, $27.66^{\circ}C$, and 0.52 g/l $KNO_3$. The biomass production at the optimal conditions was 1.55 g/l, which correlated well with the predicted value of 1.5 g/l. The total lipid and fatty acid methyl ester contents of the cells grown at the optimal conditions were 49% and 21.2% of cell dry weight, respectively. To increase the lipid content of the biomass, microalgae were challenged by nitrogen starvation. Enhancement of total lipid and fatty acid content up to 52.02% and 49%, respectively, were observed. Lipid analysis of the nitrogen-starved cells revealed that C16 and C18 species accounted for 95.9% of the total fatty acids. Among them, palmitic acid (46.17%) and oleic acid (39.43%) dominantly constituted the algal fatty acids. These results suggest Desmodesmus sp. KAERI-NJ5 as a promising feedstock for biodiesel production.

ANSIM 문서관리시스템에서 연구로사업 문서흐름

박국남(Kook-Nam Park),김권호(Kwon-Ho Kim),김준연(Jun-Yeon Kim),우상익(Sang-ik Wu),오수열(Soo-Youl Oh) 한국산업경영시스템학회 2013 한국산업경영시스템학회지 Vol.36 No.4

A document control system (DCS), ANSIM (KAERI Advanced Nuclear Safety Information Management) was designed for the purpose of documents preparation, review, and approvement for JRTR (Jordan Research and Training Reactor) project. The ANSIM system consists of a document management, document container, project management, organization management, and EPC (Engineering, Procurement and Construction) document folder. The document container folder run after specific contents, a revision history of the design documents and drawings are issued in KAERI. The EPC document work-scope is a registry for incoming documents in ANSIM, the assignment of a manager or charger, document review, preparing and outgoing PM memorandum as attached the reviewed paper. On the other hand, KAERI is aiming another extra network server for the NRR (New Research Reactor) by the end of this year. In conclusion, it is the first, computation system of DCS that provides document form, document number, and approval line. Second, ANSIM increases the productivity of performance that can be recognized the document work-flow of oneself and all participants. Finally, a plenty of experience and knowledge of nuclear technology can be transmitted to next generation for the design, manufacturing, testing, installation, and commissioning. Though this, ANSIM is expected to allow the export of a knowledge and information system as well as a research reactor.

Optimization of Phototrophic Growth and Lipid Production of a Newly Isolated Microalga, Desmodesmus sp. KAERI-NJ5

조민호,김동호,최대성,배석 한국미생물·생명공학회 2018 한국미생물·생명공학회지 Vol.46 No.4

In this study, a novel microalgal strain, Desmodesmus sp. KAERI-NJ5, was isolated, identified, and evaluated as a candidate for biodiesel feedstock. In a preliminary study, the effects of four general microalgal growth factors, including temperature, pH, light intensity, and concentration of nitrogen source (KNO3), on the microalgal photoautotrophic growth were evaluated. With the exception of light intensity, the growth factors needed to be optimized for the microalgal biomass production. Optimization was done using response surface methodology. The optimal conditions for biomass production were pH 6.54, 27.66℃, and 0.52 g/l KNO3. The biomass production at the optimal conditions was 1.55 g/l, which correlated well with the predicted value of 1.5 g/l. The total lipid and fatty acid methyl ester contents of the cells grown at the optimal conditions were 49% and 21.2% of cell dry weight, respectively. To increase the lipid content of the biomass, microalgae were challenged by nitrogen starvation. Enhancement of total lipid and fatty acid content up to 52.02% and 49%, respectively, were observed. Lipid analysis of the nitrogen-starved cells revealed that C16 and C18 species accounted for 95.9% of the total fatty acids. Among them, palmitic acid (46.17%) and oleic acid (39.43%) dominantly constituted the algal fatty acids. These results suggest Desmodesmus sp. KAERI-NJ5 as a promising feedstock for biodiesel production.

육상 섭취경로 핵종 이동인자 값에 대한 IAEA와 KAERI의 최근 동향

최용호,임광묵,전인,금동권 대한방사선방어학회 2010 대한방사선방어학회 학술발표회 논문요약집 Vol.2010 No.04

Document Management for Jordan Research and Training Reactor Project by ANSIM

박국남(Kook Nam Park),최민호(Min Ho Choi),권용세(Yong se Kwon) 한국산업경영시스템학회 2016 한국산업경영시스템학회지 Vol.39 No.2

Project management is a tool for smooth operation during a full cycle from the design to normal operation including the schedule, document, and budget management, and document management is an important work for big projects such as the JRTR (Jordan Research and Training Reactor). To manage the various large documents for a research reactor, a project management system was resolved, a project procedure manual was prepared, and a document control system was established. The ANSIM (Advanced Nuclear Safety Information Management) system consists of a document management folder, document container folder, project management folder, organization management folder, and EPC (Engineering, Procurement and Construction) document folder. First, the system composition is a computerized version of the Inter-office Correspondence (IOC), the Document Distribution for Agreement (DDA), Design Documents, and Project Manager Memorandum (PM Memo) works prepared for the research reactor design. Second, it reviews, distributes, and approves design documents in the system and approves those documents to register and supply them to the research reactor user. Third, it integrates the information of the document system-using organization and its members, as well as users' rights regarding the ANSIM document system. Throughout these functions, the ANSIM system has been contributing to the vitalization of united research. Not only did the ANSIM system realize a design document input, data load, and search system and manage KAERI's long-period experience and knowledge information properties using a manage-ment strategy, but in doing so, it also contributed to research activation and will actively help in the construction of other nuclear facilities and exports abroad.

원자력 통합안전경영시스템을 이용한 요르단연구로사업의 문서관리

Kook-Nam Park,Min-Ho Choi,Yongse Kwon 한국산업경영시스템학회 2016 한국산업경영시스템학회지 Vol.39 No.2

Project management is a tool for smooth operation during a full cycle from the design to normal operation including the schedule, document, and budget management, and document management is an important work for big projects such as the JRTR (Jordan Research and Training Reactor). To manage the various large documents for a research reactor, a project management system was resolved, a project procedure manual was prepared, and a document control system was established. The ANSIM (Advanced Nuclear Safety Information Management) system consists of a document management folder, document container folder, project management folder, organization management folder, and EPC (Engineering, Procurement and Construction) document folder. First, the system composition is a computerized version of the Inter-office Correspondence (IOC), the Document Distribution for Agreement (DDA), Design Documents, and Project Manager Memorandum (PM Memo) works prepared for the research reactor design. Second, it reviews, distributes, and approves design documents in the system and approves those documents to register and supply them to the research reactor user. Third, it integrates the information of the document system-using organization and its members, as well as users’ rights regarding the ANSIM document system. Throughout these functions, the ANSIM system has been contributing to the vitalization of united research. Not only did the ANSIM system realize a design document input, data load, and search system and manage KAERI’s long-period experience and knowledge information properties using a management strategy, but in doing so, it also contributed to research activation and will actively help in the construction of other nuclear facilities and exports abroad.

요르단연구로건설사업 문서관리시스템 구축

박국남(Kook-Nam Park),고영철(Young-Cheol Ko),우상익(Sang-Ik Wu),오수열(Soo-Youl Oh),이두정(Doo-Jeong Lee) 한국산업경영시스템학회 2011 한국산업경영시스템학회지 Vol.34 No.4

The Project of Jordan Research and Training Reactor (JRTR) officially launched in Aug. 2010. JRTR is the first made-in-Korea nuclear system to be built abroad by year 2015, and Korea Atomic Energy Research Institute (KAERI) is responsible for the design of major systems including the reactor core. While the PDCS (Project Document Control System) being operated by EPC company controls all the documents of the whole Project, KAERI is supposed to have its own system for KAERI documents. Meeting such a need, KAERI has implemented a document control for the JRTR Project into already existing ANSIM (KAERI Advanced Nuclear Safety Information Management) system. The documents of JRTR project to be controlled are defined in the PPM (Project Procedures Manual), QAP (Quality Assurance Procedure) and PEP (Project Execution Program). The ANSIM consists of the document management holder, document container holder and organization management holder. The document management holder, which is the most important part of ANSIM-JRTR, consists of the DDA (Document Distribution for Agreement), IOC (Inter-office Correspondence), PM Memo. (Project Manager Memorandum) and cover sheets of design documents. Other materials such as meeting minutes, sub-department materials and design information materials are stored in an independent COP (Community of Practice). This established computerized document control system, ANSIM, could lessen a burden for project management team and enhance the productivity as well.

(n,γ)⁹⁹Mo를 이용한 ⁹⁹Mo-^99mTc발생기의 유용성 평가

서한경,김정호,심철민,김병철,최도철,권용주,박영순,김동윤,Seo, Han Kyung,Kim, Jeong Ho,Shim, Cheol Min,Kim, Byung Cheol,Choi, Do Cheol,Gwon, Yong Ju,Park, Yung Sun,Kim, Dong Yun 대한핵의학기술학회 2013 핵의학 기술 Vol.17 No.2

본 연구의 목적은 한국원자력연구원에서 개발되고 있는 $(n,{\gamma})^{99}Mo/^{99m}Tc$발생기의 성능을 평가하고 개선방안을 연구함으로써 의학적 유효성을 확보하는 것이다. 이를 위해 본 연구에서는 한국원자력연구원에서 제조된 $(n,{\gamma})^{99}Mo/^{99m}Tc$발생기를 이용하여 $^{99m}Tc$ 용출 방사능량 시험, $^{99m}Tc$정도평가, $^{99m}Tc-DPD$, $^{99m}Tc-tetrafomin$ 표지수율 및 동물영상 평가를 진행하였다. 용출 방사능양은 상용 제품에 동일 용량에 비해 다소 적었으나, 발열성 물질 검사를 제외한 전영역의 $^{9m}Tc$정 도평가 실험에서 정상범위를 나타냈다. 표지 수율도 90% 이상 높은 수율을 관찰할 수 있었다. $^{99m}Tc-HDP$와 $^{99m}Tc-DPD$를 주입한 쥐 영상에서 한국 원자력 연구원 발생기는 모두 간과 비장의 집적을 관찰할 수 있었고 간과 비장은 상용발생기 일부에서도 관찰되었다. 이에 원자력 연구원 발생기는 발열성 물질을 제거한다면 비상시 훌륭한 대체 발생기 역할을 수행할 수 있을거라 생각한다. Purpose: The Molybdenum which is the raw material of $^{99}Mo-^{99m}Tc$ generator is produced from the nuclear reactor. However, output has dwindled as the two nuclear reactors supplying the bulk of radioactive material-one in Chalk River, Ontario and the other in Petten, the Netherlands-have been closed for repairs or maintenance. This resulted in the enhancement of its price. So $^{99}Mo-^{99m}Tc$ generator using$(n,{\gamma})^{99}Mo$ is developed by Korea Atomic Energy Research Institute (KAERI). Medicinal availability of this generator is evaluated in this study. Materials and Methods: The radioactivity of $^{99m}Tc$ eluted in generator 1, 2 and 3 unit developed by KAERI was measured. The quality control test of generator such as appearance test, pH test, LAL test, sterility test, chemical impurity (Al) test and radiochemical purity test were performed. Planar and SPECT/CT image sof SD rat (6 weeks, Female) at 2 hr after injection of $^{99m}Tc-HDP$ (hydroxymethylenediphosphonate) (TechneScan HDP, Malinckrodt Medical, Dutch) and $^{99m}Tc-DPD$ (diphosphono-1, 2-propanedicarboxylicacid) (TECEOS, CIS bio international, France) which were labeled with $^{99m}Tc$ eluted in KAERI and commercial generator (40.5 GBq, Malinckrodt Medical, Dutch) using SPECT/CT camera (Symbia, Siemense, Germany) were obtained respectively. Results: The mean radioactivity of $^{99m}Tc$ elution generator 1unit was 4.18 GBq (113 mCi), generator 2 unit was 4.73 GBq (128 mCi) and generator 3 unit was 3.33 GBq (90 mCi). All quality control tests were within normal limit except pyrogentest. Pyrogen test was positive. Planar and SPECT/CT images of rat injected $^{99m}Tc-HDP$ which was labeled with $^{99m}Tc$ eluted in commercial generator show increased uptake in bone, stomach and bowl. Planar images show increased uptake in liver and bone in case of $^{99m}Tc-DPD$. However, images of rat injected $^{99m}Tc-HDP$ and $^{99m}Tc-DPD$ which were labelled $^{99m}Tc$ eluted in KAERI generator show increased uptake in bone, liver and spleen. Conclusion: If shortcoming is removed such as pyrogen and liver appearance, domestic role as an alternative generator is thought to be able to fill and to secure the national medical service by supplying $^{99m}Tc$ when the supply of $^{99m}Tc$ be comes short.