조기진통산모에서 조직학적 융모양막염의 유무, 중증도, 양상과 양수내 interleukin-8 농도와의 연관성

장재원(Jae Weon Chang),윤보현(Bo Hyun Yoon),신희철(Hee Chul Syn) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.12

연구 목적: 양수파수없이 조기분만진통이 생긴 산모에서 양수내 IL-8과 조직학적 융모양막염의 유무, 염증 양상, 중증도와의 관련성을 확인하고자 본 연구를 시행하였다. 연구 대상 및 방법: 본 연구대상의 조건으로 1) 임신 36주 이전에 양막파수를 동반하지 않고 조기분만진통이 생겼으며, 2) 양수천자 후 5일 이내 분만되고, 3) 양수천자시 양수에서 세균배양 및 양수내 IL-8의 농도가 측정되고 분만 후 태반의 조직학적 검사가 시행되었으며, 4) 태아 및 산모의 합병증에 의한 조기분만이 아닌 경우 등으로 하였다. 이후 태반의 조직학적 검사 결과에 따른 양수내 IL-8의 농도, 양수배양결과를 Wilcoxon test for sensored data, Chi-square test for trend를 통해 비교하였다. 연구 결과: 양수배양 결과 양성인 경우는 17.4%(8/46), 조직학적 융모양막염이 있었는 경우는 63.0%(29/46)이었다. 조직학적 융모양막염이 가장 많이 발생한 부위는 융모탈락막(56.5%, 26/46)이었다. 태반염증을 부위별로 나뉘었을 때 양수내 IL-8의 농도는 양막, 융모탈락막, 제대, 융모막판의 염증 정도가 심할수록 모두에서 의미있게 높았으며(p<0.01), 전체 태반 염증 정도(total grade)가 증가할수록 양수내 IL-8의 농도는 증가하였다(p<0.001). 조직학적 융모양막염의 양상을 non-marginating, marginating, mixed로 나뉘었을 때 양수배양결과는 각각의 염증양상과 차이가 없었으나, 양수내 IL-8의 농도는 non-marginating 양상에 비해 marginating, mixed 양상의 경우 의미있게 높았으며(p<0.05), 전체 태반 염증 정도(total grade)가 grade 4 이상인 심한 조직학적 융모양막염의 빈도도 non-marginating 양상에 비해 marginating, mixed 양상에서 높았다(p<0.05). 결론: 1) 양막, 융모탈락막, 제대, 융모막판 모두에서 염증의 정도가 심한 경우, 2) marginating, mixed 염증 양상의 경우, 3) 전체 태반 염증 정도(total grade)가 높은 경우에서 양수내 IL-8의 농도가 높았으므로, 조직학적 융모양막염의 유무, 염증양상, 중증도와 양수내 IL-8 농도와의 연관성을 확인할 수 있었다. Objective: To evaluate the relationship between amniotic fluid interleukin-8 and the presence, severity and pattern of acute inflammatory lesions in the placenta delivered after preterm labor with intact membranes. Methods: The relationship between placental histologic finding and amniotic fluid interleukin-8 was examined in 46 consecutive patients who were admitted with the diagnosis of preterm labor with intact membranes and who delivered singleton gestations within 5 days. Results: The prevalence of acute histologic chorioamnionitis was 63.0%(29/46) and that of positive amniotic fluid culture was 17.4%(8/46). The most frequent site of histologic inflammation was chorion-decidua(56.5%, 26/46). The median amniotic fluid interleukin-8 increased significantly according to the presence and higher severity of inflammation in each type of placental section (p<0.05 for each). Three patterns of inflammation were identified in chorion-decidua: non-marginating, marginating, and mixed. Median amniotic fluid interleukin-8 and the rate of severe histologic chorioamnionitis (grade≥4) increased significantly in the order of non-marginating, marginating, and mixed (p<0.05 for each). Conclusion: Both the presence and greater severity of acute histologic chorioamnionitis are associated with an elevated amniotic fluid interleukin-8. A marginating and mixed pattern of inflammation are associated with a higher amniotic fluid interleukin-8. Amniotic fluid interleukin-8 is a reliable prenatal marker of histologic chorioamnionitis.

Production of Interleukin-5, Interleukin-13 and Interferon-γ in Peripheral Blood CD8+ T Cells from Children with Wheezing

이문희,문세나,장지현,정명숙,김유진,김현희,이준성,윤종서 대한 소아알레르기 호흡기학회 2008 Allergy Asthma & Respiratory Disease Vol.18 No.3

Purpose : Our objective was to investigate the role of CD8+ T cells in pathogenesis of wheezing in children with atopic nature. Methods : Twelve atopic wheezers, 8 nonatopic wheezers, 8 disease controls and 8 healthy controls were enrolled in the study. We isolated CD8+ T cells from peripheral blood samples, incubated them for 72 hours either in the absence or presence of phytohemagglutinin (PHA) and compared the concentrations of interleukin (IL)-5, IL-13, and interferon (IFN)-γ in the cell culture supernatants. Results : In the atopic wheezer group, the IL-5 concentration was significantly higher after PHA stimulation than after non-stimulated incubation. This difference was not observed in the nonatopic wheezer, disease control and healthy control groups. IL-13 was undetectable in all of the cell culture supernatants. There was no significant difference in the IFN-γ concentration between the PHA-stimulated and non-stimulated conditions in all 4 groups. Conclusion : The results of this study suggest that CD8+ T cells may play a role in the pathogenesis of wheezing in children with atopic nature through the production of IL-5. Purpose : Our objective was to investigate the role of CD8+ T cells in pathogenesis of wheezing in children with atopic nature. Methods : Twelve atopic wheezers, 8 nonatopic wheezers, 8 disease controls and 8 healthy controls were enrolled in the study. We isolated CD8+ T cells from peripheral blood samples, incubated them for 72 hours either in the absence or presence of phytohemagglutinin (PHA) and compared the concentrations of interleukin (IL)-5, IL-13, and interferon (IFN)-γ in the cell culture supernatants. Results : In the atopic wheezer group, the IL-5 concentration was significantly higher after PHA stimulation than after non-stimulated incubation. This difference was not observed in the nonatopic wheezer, disease control and healthy control groups. IL-13 was undetectable in all of the cell culture supernatants. There was no significant difference in the IFN-γ concentration between the PHA-stimulated and non-stimulated conditions in all 4 groups. Conclusion : The results of this study suggest that CD8+ T cells may play a role in the pathogenesis of wheezing in children with atopic nature through the production of IL-5.

Heilcobacter pylori 수용성 단백질에 의해 활성화된 인체 호중구의 interleukin-8 발현

김주성,정현채,김정목,송인성,김정룡 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.4

The effect of neuropeptides on secretion of Interleukin-8(IL-8)

Kim, Kyung-Jun,Park, Sang-Hyuk,Choi, Kyoung-Kyu,Park, Sang-Jin 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.3

본 연구는 치수조직, 치은, 치주인대로부터 배양된 조직을 SP (Substance P)로 4시간, SP, CGRP (Calcitonin Gene-related Peptide), Tumor necrosis factor-α (TNF-α)로 8시간 자극 후 RNase Protection Assay를 시행하고, IL-8의 분비량을 측정해 다음 결과를 얻었다. 1. IL-8 mRNA는 모든세포에서 발현됐다. 2. IL-8 mRNA 발현은 SP (10^(-5)M)와 SP (10^(-8)M)로 4시간 자극 시 증가되지 않았다. 3. IL-8 mRNA 발현은 SP (10^(-4)M)와 CGRP (10^(-6)M)로 8시간 자극 시 증가되지 않았다. 4. TNF-α (2 ng/㎖) 자극 시, IL-8 mRNA 발현이 증가됐다. 5. 치은 세포를 CGRP (10^(-6)M)로 8시간 자극 시, IL-8 분비량이 증가했다 (p < 0.05). 6. 치주인대 세포를 SP (10^(-4)M)로 8시간 자극 시 IL-8 분비량이 증가했다 (p < 0.05). We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted instact 3rd molars. Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05. According to this study, the results were as follows: 1. IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF). 2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10^(-5)M) and SP (10^(-8)M) compared with Mock stimulation in all type of cells studied. 3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10^(-4)M) and CGRP (10^(-6)M) compared with Mock stimulation in all type of cells studied. 4. TNF-α (2 ng/㎖) increased the expression of IL-8 mRNA in all kind of cells studied. 5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10^(-6)M) (p < 0.05). 6. The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10^(-4)M) (p < 0.05). Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

천명이 있는 소아의 CD8+ T 세포의 IL-5, IL-13, IFN-γ 생산

이문희 ( Moon Hee Lee ),문세나 ( Se Na Moon ),장지현 ( Ji Hyun Jang ),정명숙 ( Myung Sook Chung ),김유진 ( Eu Gene Kim ),윤종서 ( Jong Seo Yoon ),김현희 ( Hyun Hee Kim ),이준성 ( Joon Sung Lee ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2008 소아알레르기 및 호흡기학회지 Vol.18 No.3

목적: 소아 천명 환자에서 CD8+ T 세포의 IL-5, IL-13, 그리고 IFN-γ의 생산을 비교하여 천명성 질환의 병인에 있어서 CD8+ T 세포의 역할에 대한 이해의 폭을 넓히고자 하였다. 방법: 천명을 주소로 입원한 소아 환자를 대상으로 하여 알레르겐 특이 IgE의 양성여부를 기준으로 아토피성 천명군과 비아토피성 천명군으로 분류하였다. 반복적인 하기도 감염의 병력이 있으나 천명의 병력은 없었던 소아환자를 질병 대조군으로 하고, 비감염성 비염증성 질환으로 입원한 비아토피 소아 환자를 건강 대조군으로 하였다. 말초혈액에서 CD8+ T 세포를 분리하여 phytohemagglutinin (PHA)과 함께 또는 PHA 없이 72시간 동안 시험관에서 배양하였다. 배양 상층액에서 IL-5, IL-13과 IFN-γ의 농도를 측정하였다. 결과: 아토피성 천명군에서 PHA와 함께 배양된 CD8+ T 세포로부터의 IL-5의 생산이 PHA없이 배양된 경우보다 높았다. 비아토피성 천명군, 질병 대조군, 건강 대조군에서는 CD8+ T 세포의 IL-5 생산이 PHA 자극 유무에 따라 다르지 않았다. IL-13의 농도는 모든 CD8+ T 세포 배양 상층액에서 측정되지 않았다. 모든 군에서 CD8+ T 세포로부터 IFN-γ의 생산은 PHA 자극 유무에 따라 유의한 차이가 없었다. 결론: CD8+ T 세포는 IL-5의 생산을 통하여 아토피 소인을 가진 소아의 천명 발생에 기여할 가능성이 있다. Purpose: Our objective was to investigate the role of CD8+ T cells in pathogenesis of wheezing in children with atopic nature. Methods: Twelve atopic wheezers, 8 nonatopic wheezers, 8 disease controls and 8 healthy controls were enrolled in the study. We isolated CD8+ T cells from peripheral blood samples, incubated them for 72 hours either in the absence or presence of phytohemagglutinin (PHA) and compared the concentrations of interleukin (IL)-5, IL-13, and interferon (IFN)-γ in the cell culture supernatants. Results: In the atopic wheezer group, the IL-5 concentration was significantly higher after PHA stimulation than after non-stimulated incubation. This difference was not observed in the nonatopic wheezer, disease control and healthy control groups. IL-13 was undetectable in all of the cell culture supernatants. There was no significant difference in the IFN-γ concentration between the PHA-stimulated and non-stimulated conditions in all 4 groups. Conclusion: The results of this study suggest that CD8+ T cells may play a role in the pathogenesis of wheezing in children with atopic nature through the production of IL-5. [Pediatr Allergy Respir Dis(Korea) 2008;18: 228-235]

Prevotella intermedia 및 Prevotella nigrescens의 지질다당질이 대식 세포에서의 Interleukin-8 생성에 미치는 영향

김성조,Kim, Sung-Jo 대한치주과학회 2009 Journal of Periodontal & Implant Science Vol.39 No.2

Purpose: Interleukin-8 (IL-8) is an important mediator of immune and inflammatory reactions and is produced by a variety of different cell types. This study was undertaken to investigate the effects of lipopolysaccharides (LPSs) from Prevotella intermedia and Prevotella nigrescens, the major causes of inflammatory periodontal disease, on the production of IL-8 and the expression of IL-8 mRNA in differentiated THP-1 cells, a human monocytic cell line. Methods: LPSs from P. intermedia ATCC 25611 and P. nigrescens ATCC 33563 were prepared by the standard hot phenol-water method. THP-1 cells were incubated in the medium supplemented with phorbol myristate acetate to induce differentiation into macrophage-like cells. Results: We found that LPS preparations from P. intermedia and P. nigrescens can induce IL-8 mRNA expression and stimulate the release of IL-8 in differentiated THP-1 cells without additional stimuli. Conclusions: There are no previous reports of the ability of P. intermedia and P. nigrescens LPS to stimulate the release of IL-8, and the present study clearly shows, for the first time, that LPSs from P. intermedia and P. nigrescens fully induced IL-8 mRNA expression and IL-8 production in differentiated human monocytic cell line THP-1. The ability of P. intermedia and P. nigrescens LPS to promote the production of IL-8 may be important in the pathogenesis of inflammatory periodontal disease.

이질아메바에 의한 인체 대장상피세포주 HT-29에서의 interleukin-8 유전자의 발현

김정목,정현채,임경일,조양자,김정룡 INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1995 YONSEI REPORTS ON TROPICAL MEDICINE Vol.26 No.1

이질아메바에 의한 장염 환자의 조직 또는 이질아메바를 실험적으로 감염시킨 동물의 조직 검사에서 호중구의 침윤이 특징적으로 관찰된다. 그러나 이와같은 호중구의 침윤을 설명할 수 있는 기전에 대한 연구는 매우 미흡하다. 따라서 본 연구자들은 아메바 감염 초기에 인체 대장상피세포에서 interleukin-8(IL-8)이 유도되어 호중구 침윤과 같은 염증반응이 유발될 것이라는 가설을 설정하였다. 이를 위하여 인체 대장상피세포주인 HT-29에 이질아메바 영양형을 실험적으로 노출시킨 뒤 발현되는 IL-8 mRNA를 역전사 중합효소법(reverse transcriptional polymerase chain reaction, RT-PCR)으로 검사함과 동시에 발현된 IL-8 mRNA를 인공적으로 합성시킨 표준 RNA와 RT-PCR법을 이용하여 정량하였다. 실험 결과 이질아메바 영양형에 노출된 30분 후 부터 IL-8 mRNA가 발현되기 시작하였다. 그리고 그 발현 분자수는 노출 시간의 증가에 따라 계속 증가하여 3시간 대에는 3.1×10(7) molecules/㎍ total RNA를 나타내었다. 동시에 IL-8 mRNA의 발현은 노출시킨 이질아메바 영양형의 수에 비례하였다. 즉, HT-29/아메바 영양형의 비율이 10:1인 경우 IL-8 mRNA의 발현 분자수는 1.2×10(7) molecules/㎍ total RNA로 나타났다. 이와같은 IL-8 mRNA의 발현은 IL-8 단백질 분비로 이어짐을 ELISA 검사로 확인할 수 있었다. 한편 이질아메바 파쇄액(lysate)도 대장상피세포주인 Caco-2에서 IL-8 mRNA발현을 유도하였다. 결론적으로 본 실험은 이질아메바 감염 초기에 대장상피세포로 부터 IL-8이 발현되며 이에 의하여 염증반응이 촉발될 가능성이 있음을 시사해 준다. The protozoan parasite, Entamoeba histolytica, is one of major causative agents of intestinal disease all over the world. In acute experimental infection, the early host response to E. histolytica is characterized by an infiltration of neutrophils. However, the chemotactic signal for this response is not well known. Based on the finding that human epithelial cells produce the potent neutrophil chemoattractant and activator, interlukin-8 (IL-8), IL-8 gene expression was examined thoroughly in human colon epithelial cells exposed to E. histolytica trophozoites. Cellular RNAs were extracted from HT-29 or Caco-2 human colon epithelial cells exposed to E. histolytica trophozoities for 30 minutes. 1 and 3 hours. IL-8 mRNA transcripts were measured by reverse transcriptional polymerase chain reaction (RT-PCR) using synthetic standard RNA. The number of IL-8 mRNA molecules increased from 30 minutes to 3 hours of exposure period, reaching 3.1×10(7) molecules/㎍ of total RNA. Expression pattern of IL-8 mRNA transcripts was parallel to the amounts of IL-8 protein measured by enzyme-linked immunosorbent assay (ELISA). Lysates of E. histolytica also induced expression of mRNA for IL-8 in colon epithelial cells. These results suggest that acute inflammatory reaction by E. hisstolytica may be initially triggered by proinflammatory cytokines such as IL-8 secreted from epithelial cells of the colon.

Respiratory syncytial virus와 아데노바이러스에 감염된 인간기도상피세포에서 사이토카인 생산과 NF-κB 활성화의 비교

조경순 ( Kyoung Soon Cho ),이주영 ( Ju Young Lee ),윤종서 ( Jong Seo Yoon ),이윤 ( Yoon Lee ),김현희 ( Hyun Hee Kim ),이준성 ( Joon Sung Lee ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2006 소아알레르기 및 호흡기학회지 Vol.16 No.4

목적: RSV와 Ad는 영유아 호흡기질환의 가장 흔한 원인으로서 RSV는 세기관지염, 재발성 천명, 그리고 천식의 이행에 관여하고, Ad는 심한 폐손상을 유발하여 기관지확장증과 투과성폐 등 제한성 폐질환을 일으킨다. 이러한 결과는 바이러스 감염에 대한 인체의 면역반응의 차이에 기인되리라 생각된다. 저자들은 RSV와 Ad에 감염된 기관지상피세포에서 분비되는 IL-6, IL-8, RANTES의 농도를 비교하고 그런 단백질의 생산에 전사인자 NF-κB가 관여하는지를 알아보기 위하여 EMSA를 시행하였다. 방법: BEAS-2B 세포를 RSV와 Ad3, Ad7으로 감염시킨 후 배양 상층액에서 IL-6, IL-8, RANTES의 단백농도를 측정하여 감염되지 않은 대조군과 비교하고 감염된 세포의 핵단백질을 분리하여 EMSA로 NF-κB의 활성화 여부와 그 정도를 비교하였다. 결과: RSV 감염의 경우 RANTES, IL-6 및 IL-8 단백질 생산이 모두 2시간 감염 시에 나타나 48시간까지 유지된 반면(P<0.001) Ad의 경우 Ad7(MOI=100)의 경우에서만 RANTES, IL-6 및 IL-8 단백질 생산이 2시간에서 증가하여 48시간까지 유지되었고, MOI가 1인 경우에는 2시간 감염 시에만 IL-6 단백질 생산에서 통계적 유의성을 나타내었다. (P<0.001). 반면 Ad3의 경우 MOI 100에서 RANTES 단백질 생산의 경우 2시간 감염시, (P<0.05) IL-8 단백질 생산의 경우 8시간에서 증가하여 48시간까지,(P<0.05) IL-6 단백질 생산의 경우 2시간 감염시 증가하여 48시간까지 유지되어(P<0.001) 각 cytokine과 감염시간에 따라 차이를 나타내었고, MOI 1에서는 RANTES, IL-6 및 IL-8 단백질 생산 모두가 대조군에 비해 증가하지 않았다. 감염된 세포의 핵단백질에서는 감염시간에 따라 차이가 있으나 각 바이러스에 따라서도 차이가 나타났는데, RSV 감염때 RANTES, IL-6, IL-8 gene의 경우 NF-κB binding activity가 2시간에서 나타나 24시간 또는 48시간까지 유지된 반면, Ad 감염때에는 RANTES, IL-6의 경우 type이나 MOI에 따른 차이가 크게 없었고, 2시간 또는 8시간에 활성도를 나타내었다. 반면 IL-8 gene의 경우는 Ad7(MOI=100)에서만 48시간까지 유지되었다. 이러한 NF-κB 활성도는 각각 gene specific probe에 의해 억제되었다. 결론: RSV와 Ad는 BEAS-2B세포에 감염시 감염시간이나 바이러스의 종류 및 양에 따라 기도 염증을 매개하는 IL-6, IL-8, RANTES 단백의 생산을 조절하는데 그 기전에는 전사인자인 NF-κB가 중요한 역할을 한다. 이러한 바이러스들에 대한 면역반응의 차이가 각 바이러스 감염 질환의 임상 양상과 예후에 관여할 것으로 추정된다. Purpose: Our study was designed to evaluate the effects of respiratory syncytial virus (RSV) and adenovirus (Ad) on the production of interleukin (IL)-6, IL-8 and RANTES by BEAS-2B cells. The role of NF-κB in the production of those was also studied. Methods: We studied the concentration of IL-6, IL-8, RANTES in the culture supernatant of BEAS-2B cell infected by RSV, adenovirus type 3 (Ad3), Ad7. The activation of NF-κB was also investigated and the role in the production of IL-6, IL-8, RANTES compared among RSV-, Ad3-, and Ad7- infected cells by electromobility shift assay (EMSA). Results: The protein production of RANTES, IL-6, and IL-8 in RSV infection was more than Ad infection. The NF-κB binding activities in the promotor genes of RANTES, IL-6, IL-8 appeared in RSV, Ad3, and Ad7. These activities of NF-κB were inhibited by each gene specific probe. Consulsion: We suggest that such differences in the immune responses between RSV and Ad may affect the clinical patterns and prognosis after RSV and Ad infection. [Pediatr Allergy Respir Dis(Korea) 2006;15:306-316]

The effect of interleukin-8 in the early stage after anterior cruciate ligament reconstruction with remnant preservation

Kyung-Ok Kim,Jae Ang Sim,Ji-Uk Choi,Beom Koo Lee,박홍기 대한슬관절학회 2020 대한슬관절학회지 Vol.32 No.-

Purpose: We studied the effect of interleukin-8 (IL-8) as the factor for angiogenesis in the joint fluid of remnantpreserved anterior cruciate ligament reconstruction (RP-ACLR). Materials and methods: We measured 12 cytokines in joint fluid by multiplex assay and assessed the relationship between IL-8 and vascular endothelial growth factor (VEGF) concentrations. The signal intensity and mean sagittal diameter via postoperative magnetic resonance imaging (MRI) scans were evaluated and the stress X-ray image was analyzed at 3, 6, and 12 months after operation. Results: The IL-8 concentration was highest 3 months postoperatively in those patients who underwent RP-ACLR. Clinical data also showed that the signal intensity and stress radiography of the knee graft were significantly better at the early postoperative stage. Discussion: Our results show that IL-8 plays an important role in angiogenesis within 3 months after RP-ACLR. This effect yields better recovery after operation. RP-ACLR patients with high knee stability in clinical data were identical to those with high expression of IL-8 in experimental data. Therefore, IL-8 has been shown to help revascularization and ligamentization of the grafted tendon. These results indicate that IL-8 in RP-ACLR is an important factor for angiogenesis after operation. Unfortunately, the relationship of IL-8 and VEGF in vivo has not been studied. Conclusion: Our results showed that the IL-8 concentration was very high within 3 months after RP-ACLR operation. The increase in concentration of IL-8 over time was consistent with the increase in VEGF concentration. In the IL-8 clinical setting, MRI analysis showed that ACL synovialization and tension were better in patients who underwent the remnant preservation method. In addition, it was shown that RP-ACLR may be advantageous for early anterior stability within 1 year post operation and beneficial for tendon graft in the early stage post operation. Taken together, our findings suggest that IL-8 may contribute to angiogenesis which is helpful for revascularization and ligamentization of the graft tendon in the early stages of RP-ACLR.

Interleukin-8-like chemotactic factor from feline peripheral blood mononuclear cells cultured with egg white derivatives

이재권,양만표,Lee, Jae-kwon,Yang, Mhan-pyo The Korean Society of Veterinary Science 2000 大韓獸醫學會誌 Vol.40 No.2

계난백유래물질(EWD)로 배양한 고양이 말초혈액 단핵구세포(MNC)의 배양상충액에서 다형핵백혈구(PMN)에 대한 유주성인자를 조사하였다. EWD로 배양한 MNC 배양상층액과 human recombinant (hr) interleukin (IL-8)는 고양이 PMN의 유주성을 현저하게 증가시켰다. 이 유주활성물질을 규명하기 위해 EWD 처리 MNC 배양상충액을 gel electro-phoresis(denaturing 조건 18% loading gel 및 nondenaturing 조건 12.5% loading gel)를 실시한 결과, EWD로 배양한 MNC 배양상충액과 hr IL-8는 모두 분자량 6~8kDa에서 band를 나타내었다. Denaturing 조건에서 6~8kDa band의 gel slices에서 용출시킨 용출액에서도 고양이 PMN의 유주활성이 인정되었다. EWD로 배양한 MNC 배양상층액, hr IL-8 및 용출액에 의한 유도된 고양이 PMN의 유주활성은 rabbit anti-feline polyclonal IgG(RAF pIgG)와 hr IL-8에 대한 mAb에 의해 농도의존적으로 억제되었다. 또한 RAF pIgG는 hr IL-8와 결합을 보임으로써 사람의 IL-8와 교차반응을 시사하였다. 이상의 결과로부터 EWD로 배양한 고양이 MNC는 분자량 6~8kDa의 IL-8 양(樣) 유주성인자를 분비하여 PMN의 유주성을 유도하는 것으로 사료되었다. The feline chemotactic factor(s) for polymorphonuclear cells (PMN) in culture supernatant from mononuclear cells (MNC) treated with egg white derivatives (EWD) were examined. Culture supernatant from MNC treated with EWD and human recombinant (hr) IL-8 remarkably enhanced chemo-taxis of feline PMN. To investigate feline chemotactic factor(s), gel electrophoresis was performed with culture supernatant from MNC treated with EWD under denaturing (18% loading gel/5% stacking gel) and nondenaturing (12.5% loading gel/5% stacking gel) condition. Hr IL-8 and culture supernatant from MNC treated with EWD yielded a distinct band in a molecular weight, 6 to 8 kDa. Eluted solution from gel slices of 6 to 8 kDa band in denaturing condition also enhanced feline PMN chemotaxis. These chemotactic activities of feline PMN induced by culture supernatant from MNC treated with EWD, hr IL-8 and eluted solution were inhibited in a dose-dependent manner by rabbit anti-feline polyclonal IgG (RAF pIgG) and monoclonal antibody (mAb) against hr IL-8. RAF pIgG also showed a binding activity with hr IL-8, suggesting that RAF pIgG against feline IL-8-like chemotactic factor(s) had cross-reactivity with human IL-8. These results suggested that feline MNC treated with EWD might release feline IL-8-like chemotactic factor(s) with a molecular weight, 6 to 8 kDa, which induces the chemotaxis of feline PMN.