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      • KCI등재

        Identification of New Source of Resistance to Powdery Mildew of Indian Mustard and Studying Its Inheritance

        Joghee Nanjundan,Channappa Manjunatha,Jalli Radhamani,Ajay Kumar Thakur,Rashmi Yadav,Arun Kumar,Mohan Lal Meena,Rishi Kumar Tyagi,Devender Kumar Yadava,Dhiraj Singh 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.2

        Powdery mildew of Indian mustard (Brassica juncea), caused by Erysiphe cruciferarum, is emerging as ma- jor problem in India. All the Indian mustard cultivars presently grown in India are highly susceptible to pow- dery mildew and so far no resistance source has been reported. In this study, with an aim to identify resistant source, 1,020 Indian mustard accessions were evaluated against E. cruciferarum PMN isolate, at Wellington, The Nilgiris, Tamil Nadu, India under natural hot spot conditions. The study identified one accession (RDV 29) with complete resistance against E. cruciferarum PMN isolate for the first time, which was consistent in five independent evaluations. Genetic analysis of F1, F2 and backcross populations obtained from the cross RSEJ 775 (highly susceptible) × RDV 29 (highly resistant) for two season revealed that the resistance is governed by two genes with semi-dominant and gene dosage effect. Further, a new disease rating system using six scales (0, 1, 2, 3, 4, and 5) has also been proposed in this study to score powdery mildew based on progress of fungal growth in different plant parts of the F2 population. The outcome of this study viz. newly identified powdery mildew-resistant Indian mustard accession (RDV 29), information on inheritance of resistance and the newly developed disease rating scale will provide the base for development of powdery mildew-resistant cultivars of Indian mustard.

      • KCI등재

        Lead Induced Organic Acid Exudation and Citrate Enhanced Pb Uptake in Hydroponic System

        Kim, Kwon-Rae,Owens, Gary,Naidu, Ravi,Kwon, Soon-Ik,Kim, Kye-Hoon The Korean Society of Environmental Agriculture 2009 한국환경농학회지 Vol.28 No.2

        The influence of Pb-citrate complex formation on Pb uptake and the effect of Pb on organic acid exudation were investigated using four plant species, viz., sunflower (Helianthus annuus L), Indian mustard (Brassica juncea), canola (Brassica napus) and vetiver grass (Vetiveria zizanioides) under hydroponic conditions. Seedlings were exposed to different levels of Pb and Pb-citrate for 24 hrs and subsequently Pb distributions in plant shoot, root and hydroponic solution were measured. The dissolved organic carbon (DOC) concentration generally decreased as the concentration of Pb in the hydroponic solution increased. In contrast to DOC, the total organic acid concentrations exuded from Indian mustard roots significantly increased (424 to 6656 mg $kg^{-1}$) with increased Pb treatment, implying that exuding organic acids were involved in Pb accumulation in Indian mustard. The complexation of Pb with citrate enhanced Pb accumulation in the above ground portions. Lead concentration in Indian mustard increased from 2.05 mg $kg^{-1}$ to 6.42 mg $kg^{-1}$ when the concentration of citrate in solution increased from 0 to 50 mg $L^{-1}$. This result showed enhanced translocation of Pb from root to shoot with observation of transfer coefficient ($K_t$) increase from 2.03E-3 to 5.72E-3.

      • KCI등재

        Lead Induced Organic Acid Exudation and Citrate Enhanced Pb Uptake in Hydroponic System

        Kwon Rae Kim,Gary Owens,Ravi Naidu,Soon Ik Kwon,Kye Hoon Kim 한국환경농학회 2009 한국환경농학회지 Vol.28 No.2

        The influence of Pb-citrate complex formation on Pb uptake and the effect of Pb on organic acid exudation were investigated using four plant species, viz., sunflower (Helianthus annuus L), Indian mustard (Brassica juncea), canola (Brassica napus) and vetiver grass (Vetiveria zizanioides) under hydroponic conditions. Seedlings were exposed to different levels of Pb and Pb-citrate for 24 hrs and subsequently Pb distributions in plant shoot, root and hydroponic solution were measured. The dissolved organic carbon (DOC) concentration generally decreased as the concentration of Pb in the hydroponic solution increased. In contrast to DOC, the total organic acid concentrations exuded from Indian mustard roots significantly increased (424 to 6656 mg kg-1) with increased Pb treatment, implying that exuding organic acids were involved in Pb accumulation in Indian mustard. The complexation of Pb with citrate enhanced Pb accumulation in the above ground portions. Lead concentration in Indian mustard increased from 2.05 mg kg-1 to 6.42 mg kg-1 when the concentration of citrate in solution increased from 0 to 50 mg L-1. This result showed enhanced translocation of Pb from root to shoot with observation of transfer coefficient (Kt) increase from 2.03E-3 to 5.72E-3.

      • SCOPUSKCI등재

        A Comparison of Electrical Stimulation for Electrodic and EDTA-Enhanced Phytoremediation of Lead using Indian Mustard (Brassica juncea)

        Lim, Jae-Min,Jin, Biao,Butcher, David J. Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.8

        The use of plants to remove toxic metals from soil (phytoremediation) is emerging as a cost-effective alternative to conventional methods for the removal of heavy metals from contaminated soil. Indian mustard (Brassica juncea) was used as the plant to accumulate high tissue concentrations of lead when grown in contaminated soil. For this study, the application of an electric field combined effectively with EDTA-enhanced phytoremediation. A stimulation of direct and alternating electric potential was compared and EDTA-enhanced phytoremediation of lead using Indian mustard has been performed. The effects of experimental parameters such as operating voltage with different concentration of EDTA, the number of graphite electrodes, and cultivation period on the removal of toxic metal were studied. Shoot lead accumulations in Indian mustard increased as the concentration of EDTA and dc electric potential was increased. Two to four folds was increased when EDTA plus a dc electric potential was applied, compared to an ac electric potential. The maximum lead accumulation in the shoots was achieved by applying EDTA plus dc electric potential with 6 graphite electrodes.

      • KCI등재

        Effect of Indian mustard on feeding, larval survival and development of Plutella xylostella at constant temperatures

        M. Shafiq ANSARI,Tufail AHMAD,Haidar ALI 한국곤충학회 2010 Entomological Research Vol.40 No.3

        Feeding behavior of Plutella xylostella under optional to non-optional conditions was studied at 10°C, 15°C, 20°C, and 25°C on Indian mustard, Brassica juncea. The study reveals that the variety Pusa Bahar was significantly less preferred by the larvae as compared with Pusa Bold and Varuna under optional to non-optional conditions. Larvae of P. xylostella consumed more food at 25°C than 20°C, 15°C and 10°C. Larval survival was found to be highest on cabbage (control) as compared with Indian mustard and was found to vary with host plants and temperature. The larval survival decreased to 11.29% on Pusa Bahar at 10°C. Increasing the temperature from 10°C to 20°C, larval mortality resulted more on Varuna than Pusa Bahar and Pusa Bold. Developmental period was prolonged on Pusa Bold at 10°C while it was shortest on cabbage at 25°C. A total of 536.47 degree days were required to complete the development by immature stages on Varuna at 25°C and 421.64 degree days on cabbage.

      • KCI등재

        A Comparison of Electrical Stimulation for Electrodic and EDTA-Enhanced Phytoremediation of Lead using Indian Mustard (Brassica juncea)

        임재민,Biao Jin,David J. Butcher 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.8

        The use of plants to remove toxic metals from soil (phytoremediation) is emerging as a cost-effective alternative to conventional methods for the removal of heavy metals from contaminated soil. Indian mustard (Brassica juncea) was used as the plant to accumulate high tissue concentrations of lead when grown in contaminated soil. For this study, the application of an electric field combined effectively with EDTAenhanced phytoremediation. A stimulation of direct and alternating electric potential was compared and EDTA-enhanced phytoremediation of lead using Indian mustard has been performed. The effects of experimental parameters such as operating voltage with different concentration of EDTA, the number of graphite electrodes, and cultivation period on the removal of toxic metal were studied. Shoot lead accumulations in Indian mustard increased as the concentration of EDTA and dc electric potential was increased. Two to four folds was increased when EDTA plus a dc electric potential was applied, compared to an ac electric potential. The maximum lead accumulation in the shoots was achieved by applying EDTA plus dc electric potential with 6 graphite electrodes.

      • KCI등재

        Evaluation of Indian Mustard Genotypes to Heat Stress in Irrigated Environment - Seed Yield Stability and Physiological Model

        Vikrant Singh,CRam Bhajan 한국작물학회 2016 Journal of crop science and biotechnology Vol.19 No.5

        Indian mustard is mostly targeted for commercial cultivation as an early-sown or late-sown crop with the expectation of higher seed yield and oil content. With this lacuna, 45 genotypes were analyzed for yield traits by growing them as early, timely, and late-sown crops over 2 years in Pantnagar, India. The results of the ‘Eberhart and Russell Model’ and ‘GGE Biplot’ analyses were in accordance to each other but Eberhart and Russell’s model was more appropriate for judging the genotype(s) to environment specificity/adaptation while GGE Biplot was the best approach to evaluate the concerned environments for their discriminating power to genotypes. Inverse and counteracting relationships were observed among model parameters, i.e., crop growth rate (C), partitioning coefficient (P), and duration of reproductive phase (Dr) with seed yield and oil content. Seed yield was positively (P ≤ 0.01) related to all related traits except Dr, while oil content was positively (P ≤ 0.01) related to only Dr. Both C and P contributed to final yields, but P had a greater contribution particularly under terminal heat stress.

      • KCI등재

        Separation of sinigrin from Indian mustard (Brassica juncea L.) seed using macroporous ion-exchange resin

        Tianxin Wang,Qipeng Yuan,Hao Liang 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.3

        Sinigrin is a major glucosinolate present in Indian mustard (Brassica juncea L.) seeds as the precursor of the anticancer compound allyl isothiocyanate. In the present study, the adsorption and desorption characteristics of six macroporous ion-exchange resins for the separation of sinigrin from crude aqueous extracts have been compared. The results indicated that D261 resin showed the best adsorption and desorption capacity to sinigrin, and its adsorption data fit best to the Freundlich isotherm. The dynamic adsorption/desorption experiments were carried out to optimize the separation process. After treatment with D261 resin in one run, the purity of sinigrin in the product was increased 15.57-fold from 3.75% to 58.37% with the recovery of 79.82%. Meanwhile, the separation effect of D261 resin was also supported by UV and IR. The separation process using macroporous ion-exchange resin in our paper provides a novel, rapid and economical method for separation of sinigrin.

      • SCOPUSKCI등재
      • KCI등재후보

        절편부위와 사이토키닌이 갓의 재분화에 미치는 영향

        노경희,곽보경,김종범,이경렬,김현욱,김순희,강한철 한국식물생명공학회 2013 식물생명공학회지 Vol.40 No.2

        To establish an efficient protocol for plant regeneration of Brassica juncea L. Czern, the effects of explant types, explant ages and cytokinins on shoot regeneration were examined in this study. Shoot regeneration was markedly affected by the explant types used in the following order: cotyledon with petiole> hypocotyl> leaf with petiole> cotyledon> leaf. Five-day-old seedlings of cotyledon with petiole explants showed the maximum shoot regeneration frequency. Of the six cytokinins-6-γ-γ-Dimethylallylaminopurine (2-ip), 6-γ-γ-Dimethylallylamino-purine riboside (2-ip riboside), 6-Benzyl amino-purine (BAP), Thidiazuron (TDZ), Zeatin, Zeatin riboside-TDZ (8 μM) was found to be the best cytokinin for shoot regeneration with the highest shoot induction frequency (80%) from cotyledon with petiole after 4 weeks. All the regenerated plantlets were developed well and they produced morphologically normal flowers.

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