흰쥐에 조사한 자외선B가 Nitric Oxide의 활성에 미치는 효과 (I)

민경옥,김중환,Min, Kyung-Ok,Kim, Jung-Hwan 대한물리치료과학회 1999 대한물리치료과학회지 Vol.6 No.3

Recent studies have revealed that Nitric oxide(NO) was one of the demonstration for the physiological regulator, endothelial derived relaxing factor(EDRF) and that NO was produced by ultraviolet irradiation in human. Thus, the present author have carried out a experimental study on the change of hematological, histological value of ultraviolet irradiation in sprague-dawley rats. The subjects were divided into four groups of ten rats each selected at random. There were 4 groups: 1. no irradiation control; 2. ultraviolet $75mJ/cm^2$ irradiation group; 3. ultraviolet $150mJ/cm^2$ irradiation group; 4. ultraviolet $225mJ/cm^2$ group. After a irradiation, hematological and histological tests were performed to observe erythrocyte, hemoglobin, hematocrit, MCH, MCHC, MCV, $O_2$ saturation, pH, $PO_2,\;PCO_2$ value and to observe histological changes. In hematological tests, erythrocyte, hemoglobin, hematocrit significantly increased in $75mJ/cm^2$ than control group and more $150mJ/cm^2$ ultraviolet irradiation group respectively. Also In blood gas tests, $PO_2$ significantly increased in $75mJ/cm^2$ and more $150mJ/cm^2$ group than control group. Whereas $PCO_2$ significantly decreased in $75mJ/cm^2$ and more $150mJ/cm^2$ group than control group (Duncan-Tukey test, P<0.05). In histological tests, control and $75mJ/cm^2$ group unchanged, but more $150mJ/cm^2$ group changed that it was cytolysis, cytotoxic effect, acanthosis, proliferation of keratinocyte, appearance of amorphous cell and pyknotic nucleus, production of sunburn cell. In conclusion, the present author results support the importance of the relation between NO effect and hematological, histological value by ultraviolet B irradiation.

The Effect of Testicular Torsion on the Contralateral Testis in Rats : Histologic Changes and its Reversibility

Choi, Seung Kang,Lee, Young Tae Yonsei University, College of Medicine 1987 Yonsei medical journal Vol.28 No.3

연조직 결손부 회복을 위한 인공진피 이식의 조직학적 및 임상적 연구

유선열(Sun-Youl Ryu),김선국(Sun-Kook Kim) 대한구강악안면외과학회 2006 대한구강악안면외과학회지 Vol.32 No.5

The present study was aimed to compare the resorption rate and the histological change of the autogenous dermis and the artificial dermis (Terudermis??) after the transplantation, and to report the clinical results of the use of Terudermis in order to restore the soft tissue defect. Twenty mature rabbits, weighing about 2 ㎏, were used for the experimental study. The autogenous dermis and the Terudermis?? size 1 × 1 cm were transplanted to the space between the external abdominal oblique muscle and the external abdominal oblique fascia of the each rabbits. They were divided into 4 groups (n=5 each) and gathered at 1, 2, 4, and 8 weeks after the transplantation. The resorption rate was calculated, and H-E stain was preformed to observe the histological changes. The chart review of the 17 patients who received Terudermis?? graft to the facial soft tissue defects was conducted for the clinical study. The resorption rate at 8 weeks after the transplantation was 21.5% for the autogenous dermis, and 36.4% Terudermis. In microscopic examinations, the infiltration of the inflammatory cells and the epidermal inclusion cyst were observed in the autogenous dermis graft. The neovascularization and the progressive growth of the new fibroblast were shown in the Terudermis?? graft. In clinical data of 17 patients, the size of the grafted Terudermis was from 1.5 ㎠ to 7.5 ㎠ (average 3.5 ㎠). Follow-up ranged from 5 to 25 months. Fourteen patients with cleft palate demonstrated stability of the graft and unremarkable complications. But unstability of the graft and the partial relapse were observed in three patients received the vestibuloplasty. These results indicate that Terudermis can be available substitute of autogenous dermis because of the stability about resorption, the histocompatibility, and the unremarkable clinical complications.

녹용, 홍삼, 홍화, ipriflavone 및 estrogen이 난소적출 rat의 체중, 골밀도, 장기중량 및 조직학적 변화에 관한 연구

유상식 ( Sang Sik Yoo ),김상근 ( Sang Keun Kim ),이명헌 ( Myung Heun Lee ),송운재 ( Woun Jae Song ),문병천 ( Byung Cheon Moon ),김무강 ( Moo Kang Kim ) 한국동물위생학회 2000 한국동물위생학회지 (KOJVS) Vol.23 No.3

This study was performed to elucidate the effects of antler, red ginseng, safflower seed, ipriflavone and estrogen on ovariectomized rats. The rats were fed with Ca and P deficient diet for five weeks to induce osteoporosis. After this period, these animals were fed with normal feed and treated every other day with antler(600mg/kg, PO), red ginseng(200mg/kg, PO), safflower(200mg/kg, PO), ipriflavone(80mg/kg, PO) and estrogene(400μg/kg, IM) for 5 weeks. During the treatment, the rats were examined for body, organ, femur and femur ash weights. And electron-microscopical examinations were also performed on femora. The results are summarized as follows 1. The body weights of the antler, red ginseng, safflower, ipriflavone and estrogen-treated groups after five weeks of treatment, were 303.0±3.0g, 273.3±4.4g, 288.3±5.lg, 300.5± 5.2g, 320.5 ± 4.2g, respectively. They, except the red ginseng-treated group, were significantly higher than 278.4±2.7g of normal control group(P<0.05). And the body weight of non-treatment group was 294.2±5.5g which was higher than that of control group, too. 2. The weights of left femur after five weeks of treatment were showed 0.83 ± 0.2g by antler, 0.89±0.2g by red ginseng, 0.87±0.2g by safflower, 0.83±0.1g by ipriflavone and 0.78±0.lg by estrogen-treated group. They were a little lower than 0.97±0.lg of normal control group. Non-treatment group showed 0.83±0.lg which was lower than that of control group but not significant. 3. The weights of ashed left femora after five weeks of treatment were showed 0.36±0.01g by antler, 0.40 ± 0.01g by red ginseng, 0.41 ± 0.01g by safflower, 0.36 ± 0.01g by ipriflavone and 0.35 ± 0.01g by estrogen-treated group. Those results were significantly lower than 0.43 ±0.01g of normal control group. And non-treatment group showed 0.29 ±0.01g of femoral ash which was significantly lower than that showed by control group(p<0.05). 4. The femoral Ca contents of the antler, red ginseng, safflower, ipriflavone and estrogen-treated rats were 19.6±0.7%, 17.8 ± 1.1%, 18.8 ± 0.9%, 18.0±1.1%, 1 B.3 ± 1.1%, respectively, after five weeks of treatment. The femoral Ca contents of antler and safflower-treated groups were higher than that of control group which showed 18.2 ± 0.9% of Ca. The non-treatment group, showing 15.3±0.7%, had significantly lower content of Ca than normal group(p<0,05). 5. The weights of liver, spleen, and kidney in the osteoporosis-induced rats during the treatment with antler, red ginseng, safflower, ipriflavone and estrogen showed not significant changes. 6. The connections among the trabeculae of control group were maintained well, showing no bone loss. However, the connections among the trabeculae of non-treatment group were thinner than those of control group and were almost disconnected and the lacunae were found to be broadened. Antler and safflower-treated group showed only slight changes. The recovery rates were prominent in antler, safflower, estrogen-treated groups but they were insignificant in red ginseng and ipriflavone-treated groups.

자성선숙어(protogynous fish)의 성전환

이영돈,고환봉,김형배,박인석,이정재 제주대학교 해양연구소 1993 해양자원연구소연구보고 Vol.17 No.-

Studies and reviews have been based on sex reversal diagnosis of histological change and body color change by sexual dimorphism on protogynous hermaphrodite fish, Labridae, Serranidae inhabited at the costal area of Chejudo. Korea Halichorens poecilopterus, Pleragoguw flagellifera and H. teruispinis were summer season, spawing species which mainly spawn in Jun and July, but Pseudolobrus japonicus was autumn season spawing species which mainly spawn in October and November, The standard length range of sex reversal were 13.0-15.0cm H. poecilopterus 9.5-15.0cm P. flagellifer, 11.5-16.0 cm P. japonicus and 10.0-13.0cm (total length) H. teraispinis respectively. Sex reversal mainly appeared before and after spawing season. H. Poecilogterus, H. terusispirus and P. japonicus were composed of monandry and diantry, but P.flagellifera was diandry. By sex reversal, the body colors of H. poecilopterus, P.flagellifera and P. japoricus change from pale red or yellow lines to brilliant green or dark yellow lines. The body colors of H. tenurisspirnis change slight, but containe lateral stripe pattern and blue spot in body center. In P. flagellifera, 1st, 2nd spine process of dosal fin were grown with body colour change. In sex reversal process, histological change of Labridae of inner gonad showed development of various interstitial cells and blood vessel by degeneration of oocyte. Thereafter, spermatogonia and spermatocyte begin to appear at the inner edge regions of the ovarian lamellae and then converted from ovary to functional testis. Epinephelus moara of 30.0~92.3cm in TL, E. Septemfasciatus of 23.4~38.7cm in TL were female with contained oocyte of perinucleolus stage. E akaara of 22.0~33.0cm in TL appeared individuals of ovotestis.

수온과 염분이 조피볼락, Sebastes schlegeli의 생존, 대사 및 조직학적 변화에 미치는 영향

양성진(Sung Jin YANG),이정용(Jeong Young LEE),신윤경(Yun kyung SHIN),황형규(Hyung Kyu HWANG),명정인(Jeong-In MYEONG) 한국수산해양교육학회 2016 水産海洋敎育硏究 Vol.28 No.4

The suitable temperature and salinity for the long-distance transportation for the rockfish were investigated by assessing survival rate, metabolism, histological change. All experimental groups showed survival rates of 100%. Daily Oxygen consumption rhythm was decreased during nights and increased during days. Average oxygen consumption was significantly decreased as temperature and salinity were decreased. Ammonia excretion was significantly increased as temperature and salinity were decreased. Histological changes were observed in the skin and gill of the rockfish exposed to 10 psu under all the temperature conditions, with larger changes at 4℃. Further, nucleus deformation and uniformity in the cytoplasm were also observed.

Structural Alterations in the Gill of the Red Sea Bream, Pagrus major, Exposed to the Harmful Dinoflagellate Cochlodinium polykrikoides

Kim Chang Sook,Jee Bo-Young,Bae Heon Meen The Korean Society of Fisheries and Aquatic Scienc 2002 Fisheries and Aquatic Sciences Vol.5 No.1

The effects of Coehlodinium polykrikoides on the gill of red sea bream, Pagrus major, were examined to clarify the ichthyotoxic mechanisms of this plankton species. The gill of fish exposed to dense blooms over 3,000 cells/mL for 24 h showed severe epithelial separation: a severe edema was found in the secondary lamellar epithelium and interlamellar regions of primary filament. In addition, lipid peroxidation of gill tissue in Coehlodinium-exposed fish was about 2.5 times higher than that of control. The composition of glycoproteins in the gill mucus of Coehlodinium-exposed fish was also changed. These results suggest that the loss of structural integrity of cell membranes in fish gill may be deeply involved in fish death by C. polykrikoides.

실험적 뇌실질내 혈종의 외과적 치료와 주위뇌조직의 시간경과에 따른 변화

신원한 순천향대학교 1991 논문집 Vol.14 No.2

뇌실질내 혈종 주위뇌조직의 손상은 혈종 자체의 괴효과(mass effect)와 저산소증, 산성증, 뇌혈류의 감소 또는 혈액의 독성효과 등에 의하여 시간경과에 따라서 2차적으로 발생하여 진행된다고 하겠다. 저자는 시간경과에 따른 이러한 손상의 정도를 병리조직학적으로 조사 검토하여 뇌출혈 환자의 치료상에 도움을 얻고자 실험적 뇌정위수술로써 가토의 대뇌 기저핵에 뇌실질내에 혈종을 만들어서 혈종 주위조직에 미치는 시간경과에 따른 변화를 광학 및 전자 현미경적 소견으로 비교관찰하였고, 아울러 뇌실질내 혈종의 제거 시기와 제거 정도에 따른 주위뇌조직의 변화를 규명하여 뇌출혈 환자에 대한 수술적 치료의 지표를 설정하고자 가토의 뇌실질내 혈종을 만들어 일정한 시간이 지난 후에 제거수술을 시행하였고, 다시 일정한 시점에 실험동물을 희생시켜서 혈종의 제거술 시기와 제거정도에따른 주위조직 변화를 광학현미경 소견으로 비교관찰한, 그 결과는 다음과 같다. 첫째, 혈종 주위내조직의 변화는 조직의 손상인 "초기변화"와 "후기변화"로 나눌수 있었고, "초기변화"는 부종, 적혈구의 이동, 혈관주위 출혈, 괴사 및 호중구의 출현 등이고, "후기변화"는 조직의 복원 과정이라고 생각되는 대식구, 혈관의 증식과 섬유소의 출현등이며, 전자현미경의 특징적인 변화로는 축삭과 신경교세포의 변성이었다. 이들의 변화는 혈종 형성후 12시간부터 심하게 확대 진행되었다. 둘째, 수술로써 대부분의 혈종이 제거된 군에서 12시간 이전 수술한 경우에서는 주위뇌조직의 "초기와 후기변화"는 모두 약한 정도였으나, 1일 이후에 수술한 경우에서는 "초기변화"는 수술하지 않은 군과 같으며 특히 괴사의 정도는 고도로 관찰되었고, "후기변화"의 정도가 다소 경하였다. 그리고 수술로써 혈종이 부분 제거된 군에서는 주위뇌조직의 변화 정도는 혈종을 제거하지 않은 군과 별 차이가 없었다. 위의 실험결과를 토대로 뇌실질내 혈종의 수술적 치료에서 제거 시기로는 주위뇌조직의 "초기변화"의 하나인 불가역적 괴사가 심해지기 이전인 출혈후 12시간 이내로 해야하며, 혈종의 제거 정도로는 가능한 한 전부를 제거해야 할 것으로 생각된다. In an attempt to investigate the sequential histological changes in the brain tissues surrounding hematoma after the production and subsequent removal of an experimental intracerebral hematoma(ICH), an experimental model of intracerebral hematoma was made by an injection of 0.7ml of autologous whole blood with the stereotaxic frame into the right basal ganglia of the anesthetized adult rabbit. 210 adult rabbits weighing 2.8 to 3.6㎏ were divided into 3 groups : non-removal group(60 rabbits-10 subgroups) which were sacrificed in 0.5, 3, 6, 9 and 12 hours, and 1, 2, 3, 4 and 7 days after the formation of ICH for light microscopic studies, non-removal group (40 rabbits-7 subgroups) which were sacrificed in 0.5, 3, 6, 9 and 12 hours, and 1 and 3 days after the formation of ICH for eletron microscopic studies, and hematoma removal group(110 rabbits-15 subgroups) was performed by transcortical evacuation under surgical microscope at 3, 6 and 12 hours, 1 and 2 days after the formation of ICH and then were sacrificed in 6 and 12 hours, 1, 2, and 3 days after the formation of ICH for light microscopic studies. The sequential microscopic changes in the brain tissues surrounding hematoma of each subgroup were observed. The results obtained were as follows : 1. Light microscopic findings on non-removal group ; At the earlier stage, 0.5 to 24 hours after hematoma formation, edema, migration of RBCs, perivascular hemorrhage and infiltration of neurophils were observed in the surrounding brain tissues. These changes began to appear for the 3 and 6 hours groups, and were aggravated rapidly for the 12 and 24 hours groups. At the later stage, 2 to 7 days after hematoma formation, infiltration of macrophages, proliferation of vessels and fibrin were observed in the surrounding brain tissues. The process of tissue repair in these changes began on 2 days and promoted on 3 to 7 days after hematoma formation. 2. Electron microscopic findings of non-removal group ; Degeneneration of glial cell began to appear at 3 hours, and was aggravated at 12 hours, and necrosis of glial cell occurred on 1 day. Degeneration of axon began to appear at 6 hour, and was aggravated at 12 hour to 1 day, and phagocytosis of degeneratiog axon was observed on 3 days after hematoma formation. 3. Light microscopic findings of total removal group ; ⅰ) The histologic changes in the 3 and 6 hour operation groups after hematoma formation were mild in degree at the earlier and later stages, and superficial parenchymal necrosis was noted at the later stage. ⅱ) The histologic changes in the 12 hour operation group, especially necrosis, were more widened than the 6 hour operation group at the earlier stage, but other changes were mild in degree at the later stage. ⅲ) The histologic changes in the 1 and 2 day operation group were severe in degree at the earlier stage, the markedly necrotic parenchymal tissues were noted, and other changes were moderate in degree at the later stage. 4. Light microscopic findings of subtotal removal group ; There was no significant difference in histologic changes between subtotal removal group and non-removal one. These experimental data suggest that operative removal of ICH after the ictus might be carried out as quickly as possible, particuraly within 6 hours and should be done within 12 hours at the latest, before the degree and extent of necrosis in the surrounding brain tissues would be marked, and surgery after 24 hours from the onset seems to be less benefit. Surgical removal of hematoma might be performed as totally as possible, because subtotal removal of hematoma could not alleviate the anatomical extent of necrosis.

Cyclosporin A 반복투여에 의한 랫드 간장의 조직학적 변화

윤화중,손무호,장병준,권구범 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-

This study was carried out to investigate the histological changes of rat liver by repeated treatment of cyclosporin A that has been widely used for treating some autoimmune diseases and immunosuppressive drug in organ transplantation. 70 Sprague-Dawley rats were kept in experimental circumstances for 2 weeks and 50mg/kg B.W of cyclosporin A was injected once a day subcutaneously for 7 days and sacrificed at 1 hour, 1 day, 3 days, 7 days, 14 days, 28 days after the last injection. Histological changes were observed by H & E, Oil red O, and Masson trichrome stains and the results obtained were as follows. 1. 26 percent of animals has died during the treatment of cyclosporin A, and the animals survived were observed to be dull and inactivated. 2. The mild cytoplasmic changes of hepatocytes in portal area were observed by H & E stain in 1 hour lapse group and this finding was recovered with time lapse. 3. The lipid accumulation in periportal areas was observed in 1 hour and 1 day lapse groups and this finding was recovered with time lapse. 4. Although the cytoplasmic changes and lipid accumulation were started in the portal area and moved to intermediated and central areas, the changes in the intermediate and central areas were not as severe as that of portal area. 5. No specific increase of connective tissue was observed in all groups. 6. Histological changes of rat liver induced by cyclosporin A were recovered with time lapse, and the entire recovery was observed in 28 days lapse group after the last injection.

Effects of Low intensity Cardiac Rehabilitation Exercise on Weight and Histological Changes of Rat Models with Acute Myocardial Infarction

Sung Ha Ji,Ki Jong Kim 국제물리치료학회 2016 Journal of International Academy of Physical Ther Vol.7 No.1

The purpose of the study was to investigate effects of Low intensity cardiac rehabilitation, using a treadmill, on the myocardial structure. We identified the effects by analyzing changes in the rats' weights and the results of biopsies. Twenty Sprague-Dawley male rats, 50 weeks old, were randomly divided into the exercise group and the control group. myocardial infarction( MI) was induced by ligaturing their left anterior descending artery. After the acute MI induction, two rats of each group began to fall dead, therefore, eight of each group completed at the end of the experiment. We used treadmills for animals for the exercise group. This exercise group performed 30 minutes of exercise five times per week for six weeks, while the control group did not perform any exercise. No statistically significant differences in weight were found in within group comparison and between group comparison. Furthermore, we observed histological changes in the myocardium using Hematoxylin & Eosin and Masson's trichrome staining in both groups. Low-intensity exercise inhibited myocardial fibrosis, may serve as a reference in the cardiopulmonary field, which plays a role in rehabilitating patients with cardiac disorders, including acute MI.