Fumigation effect of phosphine, ethyl formate, and combined use of phosphine and ethyl formate on Tribolium castaneum (Herbst)

Ja-Eun Song,Eun-Mi Shin,Young Ju Park,Bong-su Kim,Soo-jung Suh,Jeongoh Yang 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04

The effect of fumigation on the phosphine-susceptible and -resistant strains in the T. castaneum was evaluated using phosphine, ethyl formate, and combination with phosphine and ethyl formate (phosphine+ethyl formate). The Lethal Concentration Time (LCT)50 analysis of susceptible strains, late larva showed that phosphine (0.13 mg·h / L), ethyl formate (80.91 mg·h / L), and phosphine + ethyl formate (19.36 mg·h / L). The LCT50 of adult was 0.05 mg·h / L, 68.58 mg·h / L and 17.84 mg·h / L when treated with phosphine, ethyl formate, and phosphine + ethyl formate. The LCT50 of resistant strains, late larva was found to 33.32 mg·h / L of phosphine, 113.46 mg·h / L of ethyl formate and 129.85 mg·h / L of phosphine + ethyl formate, and the LCT50 of adult was 55.71 mg·h / L of phosphine, 85.39 mg·h / L, phosphine + ethyl formate 85.83 mg·h / L. The treatment of three fumigants (phosphine, ehtyl formate, and phosphine+ethyl formate) showed the possibility of controlling against T. castaneum of phosphine-susceptible and –resistant strains.

A 90-Day Inhalation Toxicity Study of Ethyl Formate in Rats

Mi Ju Lee,Hyeon-Yeong Kim 한국독성학회 2017 Toxicological Research Vol.33 No.4

Ethyl formate, a volatile solvent, has insecticidal and fungicidal properties and is suggested as a potential fumigant for stored crop and fruit. Its primary contact route is through the respiratory tract; however, reliable repeated toxicological studies focusing on the inhalation route have not been published to date. Therefore, the present study was conducted to investigate the safety of a 90-day repeated inhalation exposure in rats. Forty male and 40 female rats were exposed to ethyl formate vapor via inhalation at concentrations of 0, 66, 330, and 1,320 ppm for 6 hr/day, 5 days a week for 13 weeks. Clinical signs, body weights, food consumption, urinalysis, hematologic parameters, serum chemistry measurements, organ weights, necropsy, and histopathological findings were compared between the control and ethyl formate-exposed groups. Locomotor activity decreased during exposure and recovered afterward in male and female rats exposed to 1,320 ppm ethyl formate. Body weight and food consumption continuously decreased in both sexes exposed to 1,320 ppm ethyl formate from week 1 or 3 compared with the control values. The increases in adrenal weight and decreases in thymus weight were noted in both sexes exposed to ethyl formate at 1,320 ppm. Degeneration, squamous metaplasia of olfactory epithelium in the nasopharyngeal tissue, or both were noted in the male and female rats at 1,320 ppm and female rats at 330 ppm ethyl formate. Taken together, our results indicate that ethyl formate-induced changes were not observed in male and female rats at 330 and 66 ppm, respectively. This indicates that exposure to ethyl formate at concentrations below 66 ppm for 90 days is relatively safe in rats. This is the first report of a full-scale repeated inhalation toxicity assessment in rats and could contribute to controlling occupational environmental hazards related to ethyl formate.

A 90-Day Inhalation Toxicity Study of Ethyl Formate in Rats

Lee, Mi Ju,Kim, Hyeon-Yeong Korean Society of ToxicologyKorea Environmental Mu 2017 Toxicological Research Vol.33 No.4

Ethyl formate, a volatile solvent, has insecticidal and fungicidal properties and is suggested as a potential fumigant for stored crop and fruit. Its primary contact route is through the respiratory tract; however, reliable repeated toxicological studies focusing on the inhalation route have not been published to date. Therefore, the present study was conducted to investigate the safety of a 90-day repeated inhalation exposure in rats. Forty male and 40 female rats were exposed to ethyl formate vapor via inhalation at concentrations of 0, 66, 330, and 1,320 ppm for 6 hr/day, 5 days a week for 13 weeks. Clinical signs, body weights, food consumption, urinalysis, hematologic parameters, serum chemistry measurements, organ weights, necropsy, and histopathological findings were compared between the control and ethyl formate-exposed groups. Locomotor activity decreased during exposure and recovered afterward in male and female rats exposed to 1,320 ppm ethyl formate. Body weight and food consumption continuously decreased in both sexes exposed to 1,320 ppm ethyl formate from week 1 or 3 compared with the control values. The increases in adrenal weight and decreases in thymus weight were noted in both sexes exposed to ethyl formate at 1,320 ppm. Degeneration, squamous metaplasia of olfactory epithelium in the nasopharyngeal tissue, or both were noted in the male and female rats at 1,320 ppm and female rats at 330 ppm ethyl formate. Taken together, our results indicate that ethyl formate-induced changes were not observed in male and female rats at 330 and 66 ppm, respectively. This indicates that exposure to ethyl formate at concentrations below 66 ppm for 90 days is relatively safe in rats. This is the first report of a full-scale repeated inhalation toxicity assessment in rats and could contribute to controlling occupational environmental hazards related to ethyl formate.

A 90-Day Inhalation Toxicity Study of Ethyl Formate in Rats

이미주,김현영 한국독성학회 2017 Toxicological Research Vol.33 No.4

Ethyl formate, a volatile solvent, has insecticidal and fungicidal properties and is suggested as a potential fumigant for stored crop and fruit. Its primary contact route is through the respiratory tract; however, reliable repeated toxicological studies focusing on the inhalation route have not been published to date. Therefore, the present study was conducted to investigate the safety of a 90-day repeated inhalation exposure in rats. Forty male and 40 female rats were exposed to ethyl formate vapor via inhalation at concentrations of 0, 66, 330, and 1,320 ppm for 6 hr/day, 5 days a week for 13 weeks. Clinical signs, body weights, food consumption, urinalysis, hematologic parameters, serum chemistry measurements, organ weights, necropsy, and histopathological findings were compared between the control and ethyl formate-exposed groups. Locomotor activity decreased during exposure and recovered afterward in male and female rats exposed to 1,320 ppm ethyl formate. Body weight and food consumption continuously decreased in both sexes exposed to 1,320 ppm ethyl formate from week 1 or 3 compared with the control values. The increases in adrenal weight and decreases in thymus weight were noted in both sexes exposed to ethyl formate at 1,320 ppm. Degeneration, squamous metaplasia of olfactory epithelium in the nasopharyngeal tissue, or both were noted in the male and female rats at 1,320 ppm and female rats at 330 ppm ethyl formate. Taken together, our results indicate that ethyl formate-induced changes were not observed in male and female rats at 330 and 66 ppm, respectively. This indicates that exposure to ethyl formate at concentrations below 66 ppm for 90 days is relatively safe in rats. This is the first report of a full-scale repeated inhalation toxicity assessment in rats and could contribute to controlling occupational environmental hazards related to ethyl formate.

복숭아혹진딧물(Myzus persicae) 약충에 대한 에틸포메이트 훈증 독성의 생화학적 메커니즘

김경남 ( Kyeongnam Kim ),이병호 ( Byung-ho Lee ),박정선 ( Jeong Sun Park ),양정오 ( Jeong Oh Yang ),이성은 ( Sung-eun Lee ) 한국응용생명화학회(구 한국농화학회) 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.3

에틸포메이트는 해충을 방제하기 위한 훈증제로서 사용되어 왔다. 그러나 농산업에서 해충을 박멸하기 위하여 사용된 이 물질이 일으키는 훈증독성의 작용점에 대해서는 많은 연구가 수행되지 않았다. 본 연구에서 저자들은 복숭아혹진딧물 약충에 에틸포메이트를 훈증 처리 하였을 때 추측 가능한 작용점들을 제시하였다. 에틸포메이트 훈증 처리 후 복숭아혹진딧물 약충에 대한 생화학 및 분자적 수준에서의 변화를 측정하였다. Cytochrome c oxidase (COX)의 활성은 에틸포메이트 훈증 처리된 복숭아혹진딧물 약충에서 약 2배 이상 증가하였다. Acetylcholinesterase (AChE)의 경우 에틸포메이트의 훈증 처리농도가 증가됨과 함께 유전자 발현이 감소되었다. 이 두 발견은 COX와 AChE가 주요한 에틸포메이트 훈증독성의 작용점임을 시사하였다. 이 결과들과 함께 MALDI-TOF MS/MS를 이용하여 지질대사체를 분석한 후 2배 이상 증감을 보인 9종 인지질들을 동정하였고 이들이 세포막 조성에 변화를 유발함을 밝혔다. 결론적으로 복숭아혹진딧물 약충에 대한 에틸포메이트의 훈증독성은 COX 활성 변화, AChE 발현 변화, 그리고 인지질의 생성 변화에 기인하였다. Ethyl formate has been used for the control of insect pests by fumigation. However, there were not many reports to show its target site of fumigant toxicity on insect pests since its first use in the agricultural industry. In the present study, we showed the presumable target sites of ethyl formate fumigation in insect pests using Myzus persicae nymphs. After ethyl formate fumigation, the nymphs of this species were collected and the changes at the biochemical and molecular level were determined. The activity of cytochrome c oxidase (COX) was approximately two-fold higher after ethyl formate fumigation. In addition, the expression levels of acetylcholinesterase (AChE) decreased gradually with increasing ethyl formate concentration. These two findings suggested that COX and AChE might be the major target sites of ethyl formate fumigation. In addition to these results, the analysis of lipid content using MALDI-TOF MS/MS identified 9 phospholipids differently generated 2-fold higher in the ethyl formate-treated nymphs than that in the control nymphs, thereby leading to changes in cell membrane composition in M. persicae nymphs. Therefore, the ethyl formate fumigation caused lethal effects on M. persicae nymphs by changing COX activity, AChE gene expression, and phospholipid production.

Biochemical mechanisms of fumigant toxicity by ethyl formate towards Myzus persicae nymphs

Kyeongnam Kim,Byung-Ho Lee,Jeong Sun Park,Jeong Oh Yang,Sung-Eun Lee 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

Ethyl formate has been used for the control of insect pests by fumigation. However, there were not many reports toshow its target site of fumigant toxicity on insect pests since its first use in the agricultural industry. In the present study,we showed the presumable target sites of ethyl formate fumigation in insect pests using Myzus persicae nymphs. Afterethyl formate fumigation, the nymphs of this species were collected and the changes at the biochemical and molecularlevel were determined. The activity of cytochrome c oxidase (COX) was approximately two-fold higher after ethyl formatefumigation. In addition, the expression levels of acetylcholinesterase (AChE) decreased gradually with increasing ethylformate concentration. These two findings suggested that COX and AChE might be the major target sites of ethyl formatefumigation. In addition to these results, the analysis of lipid content using MALDI-TOF MS/MS identified 9 phospholipidsdifferently generated 2-fold higher in the ethyl formate-treated nymphs than that in the control nymphs, thereby leadingto changes in cell membrane composition in M. persicae nymphs. Therefore, the ethyl formate fumigation caused lethaleffects on M. persicae nymphs by changing COX activity, AChE gene expression, and phospholipid production.

에틸포메이트의 비표적생물에 대한 급성독성 및 sodium silicate의 약해저감 효과

김경남,이유빈,김유림,김동현,김채은,조예린,박준영,유용하,이병호,이성은 한국환경생물학회 2023 환경생물 : 환경생물학회지 Vol.41 No.3

에틸포메이트 (Ethyl formate)는 자연상에서 발생되는 천연살충제로서 훈증방식으로 검역단계에서 해외로부터 유입되는 해충의 방제를 위하여 사용되고 있다. 특히, 메틸브로마이드의 대체제로서 주로 사용되며 인축에 독성이 약하고 식물에 대한 약해가 적다. 이러한 에틸포메이트를 농업해충방제를 훈증제로 사용하기 전에 유용생물체에 대한 독성과 수박, 애호박, 참외에 대한 식물독성 및 발생한 식물독성의 저감화 방안을 강구하고자 본 연구를 수행하였다. 에틸포메이트 2시간 훈증처리 후 지렁이, 꿀벌, 누에에 대한 LC50 값은 각각 39.9, 7.09, 17.9 g m-3였다. EF에 대한 감수성 정도는 LC50 값 기준 지렁이, 누에, 꿀벌 순으로 낮게 나타났으며 에틸포메이트 훈증처리는 꿀벌에 대하여 강한 독성을 유발하였다. 식물독성은 7.5 g m-3 농도로 처리된 수박 잎에서 관찰되었으며 10.0 g m-3 농도로 처리 시 수박 잎의 가장자리는 까맣게 타들어 가는 산에 의한 약해가 발생함을 확인하였다. 다른 박과 식물인 애호박 및 참외에는 10 g m-3 농도처리 시 잎에 강한 약해를 보였고 이를 저감하기 위하여 사용된 sodium silicate는 10% 및 20% 처리 시 약해의 저감을 확인할 수 있었다. 이와 같이 에틸포메이트 훈증처리 시 비표적생물에 대한 급성독성연구와 대상작물의 약해발생을 저감하는 연구는 에틸포메이트의 효율적인 농업해충 방제를 위한 중요한 결과를 도출했다. Ethyl formate (EF) is a naturally occurring insecticidal compound and is used to control pests introduced from abroad, in quarantine, by a fumigation method. In particular, it is mainly used as a substitute for methyl bromide and is less toxic to humans and less harmful to plants. This study aimed to investigate the possible acute toxicity of EF to useful organisms, and how to reduce phytotoxicity in watermelon, zucchini, and oriental melon. After fumigation with EF for 2 h, the LC50 values for earthworms, honey bees, and silkworms were 39.9, 7.09, and 17.9 g m-3, respectively. The degree of susceptibility to EF was in the order of earthworms, silkworms, and honey bees based on the LC50 value, and EF fumigation induced stronger acute toxicity to honey bees. Phytotoxicity was observed in watermelon leaves treated with a concentration of 7.5 g m-3 EF, and when treated with a concentration of 10.0 g m-3, it was confirmed that the edges of watermelon leaves were charred and seemed to be damaged by acids. Zucchini and melon, and other cucurbits, showed strong damage to the leaves when treated with a concentration of 10 g m-3, and sodium silicate, at concentrations of 10% and 20%, was used to reduce phytotoxicity. Therefore, acute toxicity towards nontarget organisms and phytotoxicity during the fumigation of EF should be reduced for efficient agricultural pest control.

Versatile Formate Production through Enzyme-catalyzed Carbon Monoxide Hydration Reaction

Jinhee LEE,Ho Won HWANG,Suk Min KIM,Byoung Wook JEON,Yong Hwan KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Carbon monoxide is hugely emitted from industrial processes as a part of waste gas in modern age. This research suggests the CO (carbon monoxide) hydration reaction which works at room temperature without additional energy supply and even produce versatile formate. This reaction is catalyzed by the combined action of CODH (CO dehydrogenase), FDH (formate dehydrogenase) and electron mediator. The CODH oxidizes CO to CO₂ (carbon dioxide) through water split and yields electrons which subsequently used to reduce electron mediators. Then, the reduced mediators transfer electrons to FDH catalyzing the CO₂ to formate. Isotope analysis of <SUP>13</SUP>CO and D₂O supports the production of formate from CO. As a result, no additional energy is necessary because CO was the sole source of electron as well as CO₂. Furthermore, this reaction system was robust to produce over 1 M of formate and stable reusability was shown with real LDG (Linz-Donawiz converter gas) from Hyundai Steel without any pretreatment by using mutated enzyme with enhanced O₂ tolerance.

Ethyl Docosahexaenoate and Its Acidic Form Increase Bone Formation by Induction of Osteoblast Differentiation and Inhibition of Osteoclastogenesis

( Tae Sung Bae ),( Mi Kyung Lee ),( Byung Il Kim ),( Jae Soon Eun ),( Yun Jo Soh ),( Bo Yun Choi ),( Manoj Nepal ) 한국응용약물학회 2011 Biomolecules & Therapeutics(구 응용약물학회지) Vol.19 No.1

Bone remodeling is a dynamic process involving a constant balance between osteoclast-induced bone resorption and osteoblast induced bone formation. Osteoclasts play a crucial homeostatic role in skeletal modeling and remodeling, and destroy bone in many pathological conditions. Previously, we reported that the hexane soluble fraction of Ficus carica inhibited osteoclast differentiation. Poly unsaturated fatty acids, such as ethyl docosahexaenoate (E-DHA), docosahexaenoic acid (DHA), cis-11,14-eicosadienoic acid (EDA) and eicosapentaenoic acid (EPA), were identified from the hexane soluble fraction of Ficus carica. Among them, E-DHA most potently inhibited osteoclastogenesis in RAW264.7 cells. E-DHA reduced the activities of JNK and NF-KB. E-DHA suppressed the expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1). Interestingly, DHA increased the activity of alkaline phosphatase and expression of bone morphogenetic protein 2 (BMP2) more than E-DHA in MC3T3-E1 cells, suggesting that DHA may induce osteoblast differentiation. The data suggests that a combination of E-DHA and DHA has potential use in the treatment of diseases involving abnormal bone lysis, such as osteoporosis, rheumatoid arthritis and periodontal bone erosion.

저곡해충 가루좀벌레에 대한 Ethyl formate와 Phosphine 병행처리 효과

이장훈,조진훈,홍진표,심재훈,이한영 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

국내 정부양곡 보관창고에서는 알루미늄포스파이드 정제를 사용하여 쌀 해충 방제를 실시하고 있다. 이 약제는훈증시간이 4~7일로 장시간 소요되며, 제형 특성상 분말(잔사물)제거가 필요하고, 훈증완료 후에도 3~5%의 미반응물질이 남아있는 것으로 알려져 있다. 따라서 신속하고 안전한 새로운 훈증기술개발이 필요한 실정이며, Methylbromide(MB) 대체훈증제인 Ethyl formate(EF)와 Phosphine(PH3)의 병행처리를 통해 국내 주요 저곡해충인 가루좀벌레(장두)에 대한 약효를 조사하였다.가루좀벌레 성충을 대상으로 데시케이터 실험을 통해 도출된 100% 방제 가능 조건인 EF+PH3(16.6 + 1g/m3),24시간을 금속훈증상(1m3)에서 처리한 결과 가루좀벌레에 대해 100% 방제효과를 확인하였다. 본 연구는 양곡보관창고 훈증처리시 알루미늄포스파이드 대체제로서 EF+PH3의 사용 가능성을 제시하였다.