Probe-based Charge Injection Study of DNA Charge Transfer for Applications to Molecular Electro-optic Switching

류호정(Hojeong Ryu),김희영(Heeyoung Kim),김동현(Donghyun Kim) 大韓電子工學會 2011 電子工學會論文誌-SC (System and control) Vol.48 No.3

본 논문에서는 DNA 올리고뉴클리오타이드(oligonucleotide)를 통한 전하 이동을 기반으로 하는 분자성 전자광학 스위칭 소자를 제시한다. DNA 올리고머(oligomer)가 흡착되어 있는 금전극에 전자들이 주입되어 전극으로부터 DNA 올리고머로 전하가 흘러가게 하고 이 전하의 이동도를 광학적 스위칭으로 확인할 수 있도록 제안되었다. DNA 올리고머의 흡착량이 증가함에 따라 DNA를 통한 전하의 이동성과 전극 표면에서의 전하전달 제한성으로 인해 전리전류는 감소하였다. DNA의 끝단에 합성된 Cy3 형광 분자의 점멸도를 전극 기반의 전하 주입법을 이용하여 확인하였다. 이러한 결과들은 DNA 올리고머를 이용한 새로운 분자성 전자광학 스위칭 소자에 이용될 수 있다. Charge transfer through DNA oligonucleotides has been investigated for potential applications of DNA into molecular electrooptic switching devices. Electrons were injected using gold electrode probes where DNA oligomers were adsorbed that are separated in medium. The results show that increased adsorption of DNA reduces the ionization current due to the combined effect of charge transfer through DNA and surface-limited charge transport. The probe-based charge injection was extended to examine the capability of extinguishing fluorescence of Cy3 dye molecules attached to DNA. It is expected that the results may be employed to implementing a novel electrooptic switching device based on DNA molecules.

Probe-based Charge Injection Study of DNA Charge Transfer for Applications to Molecular Electro-optic Switching

류호정,김희영,김동현,Ryu, Ho-Jeong,Kim, Hee-Young,Kim, Dong-Hyun The Institute of Electronics and Information Engin 2011 電子工學會論文誌-SC (System and control) Vol.41 No.4

Charge transfer through DNA oligonucleotides has been investigated for potential applications of DNA into molecular electrooptic switching devices. Electrons were injected using gold electrode probes where DNA oligomers were adsorbed that are separated in medium. The results show that increased adsorption of DNA reduces the ionization current due to the combined effect of charge transfer through DNA and surface-limited charge transport. The probe-based charge injection was extended to examine the capability of extinguishing fluorescence of Cy3 dye molecules attached to DNA. It is expected that the results may be employed to implementing a novel electrooptic switching device based on DNA molecules. 본 논문에서는 DNA 올리고뉴클리오타이드(oligonucleotide)를 통한 전하 이동을 기반으로 하는 분자성 전자광학 스위칭 소자를 제시한다. DNA 올리고머(oligomer)가 흡착되어 있는 금전극에 전자들이 주입되어 전극으로부터 DNA 올리고머로 전하가 흘러가게 하고 이 전하의 이동도를 광학적 스위칭으로 확인할 수 있도록 제안되었다. DNA 올리고머의 흡착량이 증가함에 따라 DNA를 통한 전하의 이동성과 전극 표면에서의 전하전달 제한성으로 인해 전리전류는 감소하였다. DNA의 끝단에 합성된 Cy3 형광 분자의 점멸도를 전극 기반의 전하 주입법을 이용하여 확인하였다. 이러한 결과들은 DNA 올리고머를 이용한 새로운 분자성 전자광학 스위칭 소자에 이용될 수 있다.

Intratesticular Injection of Hypertonic Saline : Non-Invasive Alternative Method for Animal Castration Model

Byung Kuk Kwak,Sung-Ho Lee 한국발생생물학회 2013 발생과 생식 Vol.17 No.4

Previous studies, including our own, have demonstrated that the intratesticular injection of hypertonic saline (20%) decreased serum testosterone level which was similar to the surgical castration in the rat, showing the state of chemical castration. In the present study, we further verify the efficacy of this less invasive method as an alternative of surgical orchidectomy in the andrological field. Sterilized 20% saline was directly injected into the adult male rats (750 μl per testis). The tested rats were divided into 3 groups including intact group (intact), orchidectomy group (ORX) and saline injection group (SAL) after bilateral orchidectomy was performed at the same day of injection. All rats were sacrificed at 4 weeks after injection. The reproductive organs (testes, epididymis, seminal vesicles and prostates) were collected and used for DNA and protein pattern analyses. Also, patho-histological studies on the testes were performed. In contrast to the intact group, similar DNA damages of testis and seminal vesicle were appeared in ORX group and SAL group. The DNA degradations seemed to be the results of necrosis rather than apoptosis. In the protein pattern analysis, all the testing tissues exerted similar patterns in the ORX group and the SAL group compared to the those of intact group. Patho-histological studies revealed that severe degenerative changes in testicular seminiferous tubules and massive infiltration of immune cells in SAL group. The present study confirmed that direct injection of hypertonic saline into the testis caused the equivalent biochemical changes in the accessory sex organs as shown in the orchidectomized animals. These results suggest that hypertonic saline injection model could be a useful castration model which can substitute for surgical castration when its safety is secured through further study in the future.

Intratesticular Injection of Hypertonic Saline: Non-Invasive Alternative Method for Animal Castration Model

Kwak, Byung Kuk,Lee, Sung-Ho The Korean Society of Developmental Biology 2013 발생과 생식 Vol.17 No.4

Previous studies, including our own, have demonstrated that the intratesticular injection of hypertonic saline (20%) decreased serum testosterone level which was similar to the surgical castration in the rat, showing the state of chemical castration. In the present study, we further verify the efficacy of this less invasive method as an alternative of surgical orchidectomy in the andrological field. Sterilized 20% saline was directly injected into the adult male rats (750 ${\mu}l$ per testis). The tested rats were divided into 3 groups including intact group (intact), orchidectomy group (ORX) and saline injection group (SAL) after bilateral orchidectomy was performed at the same day of injection. All rats were sacrificed at 4 weeks after injection. The reproductive organs (testes, epididymis, seminal vesicles and prostates) were collected and used for DNA and protein pattern analyses. Also, patho-histological studies on the testes were performed. In contrast to the intact group, similar DNA damages of testis and seminal vesicle were appeared in ORX group and SAL group. The DNA degradations seemed to be the results of necrosis rather than apoptosis. In the protein pattern analysis, all the testing tissues exerted similar patterns in the ORX group and the SAL group compared to the those of intact group. Patho-histological studies revealed that severe degenerative changes in testicular seminiferous tubules and massive infiltration of immune cells in SAL group. The present study confirmed that direct injection of hypertonic saline into the testis caused the equivalent biochemical changes in the accessory sex organs as shown in the orchidectomized animals. These results suggest that hypertonic saline injection model could be a useful castration model which can substitute for surgical castration when its safety is secured through further study in the future.

Intratesticular Injection of Hypertonic Saline : Non-Invasive Alternative Method for Animal Castration Model

곽병국,이성호 한국발생생물학회 2013 발생과 생식 Vol.17 No.4

Previous studies, including our own, have demonstrated that the intratesticular injection of hypertonic saline (20%) decreased serum testosterone level which was similar to the surgical castration in the rat, showing the state of chemical castration. In the present study, we further verify the efficacy of this less invasive method as an alternative of surgical orchidectomy in the andrological field. Sterilized 20% saline was directly injected into the adult male rats (750 μl per testis). The tested rats were divided into 3 groups including intact group (intact), orchidectomy group (ORX) and saline injection group (SAL) after bilateral orchidectomy was performed at the same day of injection. All rats were sacrificed at 4 weeks after injection. The reproductive organs (testes, epididymis, seminal vesicles and prostates) were collected and used for DNA and protein pattern analyses. Also, patho-histological studies on the testes were performed. In contrast to the intact group, similar DNA damages of testis and seminal vesicle were appeared in ORX group and SAL group. The DNA degradations seemed to be the results of necrosis rather than apoptosis. In the protein pattern analysis, all the testing tissues exerted similar patterns in the ORX group and the SAL group compared to the those of intact group. Patho-histological studies revealed that severe degenerative changes in testicular seminiferous tubules and massive infiltration of immune cells in SAL group. The present study confirmed that direct injection of hypertonic saline into the testis caused the equivalent biochemical changes in the accessory sex organs as shown in the orchidectomized animals. These results suggest that hypertonic saline injection model could be a useful castration model which can substitute for surgical castration when its safety is secured through further study in the future.

DNA 검출 공정 전용 플라스틱 튜브형 시험관 개발에 관한 연구

최규완,라문우,강정희,장성호,Choi, Kyu-wan,La, Moon-woo,Gang, Jung-hee,Chang, Sung-ho 한국금형공학회 2017 한국금형공학회지 Vol.11 No.3

PCR(Polymerase chain reaction) is a technique to replicate and amplify a desired part of DNA. It is used in various aspects such as DNA fingerprint analysis and rare DNA amplification of an extinct animal. Especially in the medical diagnosis field, it provides various measurement methods at the molecular level such as genetic diagnosis, and is a basic tool for molecular diagnostics. The internal shape of the plastic vial tube for PCR analysis used in the DNA detection process, and the surface roughness and internal cleanliness can affect detection and discrimination results. The plastic vial tube demanded by the developer of the PCR analysis equipment should be changed to a structure that eliminates the residual washing solution in the washing process to ensure the internal cleanliness. Thus the internal structure and the internal surface design for improving the PCR amplification efficiency are key issues to develop the plastic vial tube for the DNA detection process.

Induction of Genetic Variants of Horticultural Crops by DNA Injection, Protoplasts and Embryo Culture

KANG, NAM JUN,PARK, CHAN HEE,CHO, JEOUNG LAI,KIM, ZHOO HYEON,PARK, JUNG CHOON,KANG, SEONG MO 경상대학교 유전공학연구소 1987 遺傳工學硏究所報 Vol.6 No.-

Genetic transformation was induced by microinjection and co-cultivation techniques. Total DNA extracted from Altari(A) radish leaves was microinjected into the ovary of Kungzung(K) radish when the recipient was self-pollinated. As judged by their hypocotyl colors and leaf types. seven plants out of 2,760 were found transformed when the seeds harvested from the treated K plants were grown(TS-0 generation). When the seeds from those 7 transformants were grown again (Ts-1 generation), the hypocotyl colors, the number of parted leaflets, and leaf margins were segregated to the ratio of 3 to 1 as if the TS-1 plants were the hybrids between A adn K. The ratio suggested that each of such characters was determined by a single gene, and those genes were stably incorporated into the recipient genome. The transformation was also demonstrated at the seed stage by comparing the banding patterns of not only total proteins but the isozymes of malate dehydrogenase, urease, and acid phosphatase. Some transformants have been obtained by co-cultivating immature K embryos with the DNA prepared from A leaves. The optimum conditions have been established to culture tomato protoplasts in the presence of potato leaf DNA. Production of genetic variants in this study implies that such techniques could be a way to bypass the vector system in transformation studies and the genetic barrier could be overcome at the molecular level.

Fundamental study on gene transfer utilizing magnetic force and jet injector

Hasegawa, T.,Nakagam, H.,Akiyama, Y.,Nishijima, S. The Korea Institute of Applied Superconductivity a 2017 한국초전도저온공학회논문지 Vol.19 No.1

Recently, DNA vaccination is attracting attentions as a new therapeutic method for lifestyle diseases and autoimmune diseases. However, its clinical applications are limited because a safe and efficient gene transfer method has not been established yet. In this study, a new method of gene transfer was proposed which utilizes the jet injection and the magnetic transfection. The jet injection is a method to inject medical liquid by momentary high pressure without needle. The injected liquid diffuses in the bio tissue and the endocytosis is considered to be improved by the diffusion. The magnetic transfection is a method to deliver the conjugates of plasmid DNA and magnetic particles to the desired site by external magnetic field. It is expected that jet injection of the conjugates causes slight membrane disruptions and the traction of the conjugates by magnetic field induces the efficient gene transfer. In conclusion, the possibility of improvement of the gene expression by the combination of jet injection and magnetic transfection was confirmed.

Fundamental study on gene transfer utilizing magnetic force and jet injector

T. Hasegawa,H. Nakagami,Y. Akiyama,S. Nishijima 한국초전도저온학회 (구 한국초전도저온공학회) 2017 한국초전도저온공학회논문지 Vol.19 No.1

Genetic Stability of the Characters Transformed by Microinjection of Exogenous DNA into Crop Plants

KANG, NAM-JUN,PARK, CHAN-HEE,JEONG, YEON-OK,CHO, JEOUNG-LAI,KIM, ZHOO-HYEON,UM, SUNG-KYUN,PARK, JUNG-CHOON,KANG, SEONG-MO 경상대학교 유전공학연구소 1988 遺傳工學硏究所報 Vol.7 No.-

Genetic stability was examined for the second generation(TS-2) progenies of 4 transformants induced by injecting Altari(A) DNA into kungzung(K) radish. All TS-2 plants originated from T3 and T4 had red hypocotyls, suggesting the stable incorporation of the foreign gene and its expression at the second generation. The progenies of these 2 transformants had bigger leaves and heavier roots. However, the segregation of hypocotyl colors of TS-2 plants from T5 and T7 was variable, although the majority still had red hypocotyls. Except the progenises derived from T7, the shapes of cotyledons and the first true leaf, and overall morphology of TS-2 plants as well, were of A types as long as their hypocotyl color was red. For the seedlings to have red hypocotyls, the banding patterns of total suluble proteins and the isozymes of MDH and acid phosphatase should be similar to those of A radish, or at least a mixed type of A and K radish. The later the embryos were removed from the mother plants, and the higher the concn of exogenous DNA up to 10㎍ embryo, the better the differentiation of the explants. The probability to porduce intergeneric hybrids between radish and Chinese cabbage (CC) depended on which was being served as a female. The hybrid plants tended to have mixed banding patterns of total proteins and the isozyme when CC served as a female