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Ciceribacter azotifigens sp. nov., a nitrogen-fixing bacterium isolated from activated sludge
Siddiqi, Muhammad Zubair,Choi, Gyu-Min,Im, Wan-Taek Society for General Microbiology 2018 International journal of systematic and evolutiona Vol.68 No.2
<P>A Gram-reaction-negative, catalase-and oxidase-positive, aerobic, transparent, motile and rod-shaped bacterium that was capable of fixing dinitrogen (designated strain A.slu09(T)), isolated from activated sludge, was characterized by a polyphasic approach to clarify its taxonomic position. Strain A.slu09(T) was observed to grow optimally at 30 degrees C and at pH 7.0 on R2A agar medium. Strain A.slu09(T) showed beta-glucosidase activity, converting the major ginsenoside Rd to ginsenoside F2. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A.slu09(T) belongs to the genus Ciceribacter of the family Rhizobiaceae and was most closely related to Ciceribacter lividus MSSRFBL1(T) (97.8% similarity). The DNA G+C content was 67.2 mol%. The DNA-DNA hybridization value between strain A.slu09(T) and C. lividus KCTC 32403(T) was 16.9 +/- 1.17 %. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, aminophospholipid and two glycolipids, and one unknown phospholipid as a minor lipid. The predominant quinone was ubiquinone-10 (Q-10). The major fatty acids were C-19 : 0 cyclo omega 8c, C-18 : 1 omega 7c and/or C-18 : 1 omega 6c (summed feature 8) and C-18 : 0, a profile that supported the affiliation of A. slu09(T) to the genus Ciceribacter. Moreover, the physiological and biochemical characteristics and low level of DNA-DNA relatedness allowed the phenotypic and genotypic differentiation of strain A.slu09(T) from the recognized species of the genus Ciceribacter. Therefore, strain A.slu09(T) represents a novel species of the genus Ciceribacter, for which the name Ciceribacter azotifigens sp. nov. is proposed. The type strain is A.slu09(T) (=KACC 19080(T)=LMG 29962(T)).</P>
Tongchu Deng,Youfen Qian,Xingjuan Chen,Xunan Yang,Jun Guo,Guoping Sun,Meiying Xu 한국미생물학회 2020 The journal of microbiology Vol.58 No.5
A nitrate-reducing Fe(II)-oxidizing bacterial strain, F8825T, was isolated from the Fe(II)-rich sediment of an urban creek in Pearl River Delta, China. The strain was Gram-negative, facultative chemolithotrophic, facultative anaerobic, nonspore- forming, and rod-shaped with a single flagellum. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that it belongs to the genus Ciceribacter and is most closely related to C. lividus MSSRFBL1T (99.4%), followed by C. thiooxidans F43bT (98.8%) and C. azotifigens A.slu09T (98.0%). Fatty acid, polar lipid, respiratory quinone, and DNA G + C content analyses supported its classification in the genus Ciceribacter. Multilocus sequence analysis of concatenated 16S rRNA, atpD, glnII, gyrB, recA, and thrC suggested that the isolate was a novel species. DNA–DNA hybridization and genome sequence comparisons (90.88 and 89.86%, for values of ANIm and ANIb between strains F8825T with MSSRFBL1T, respectively) confirmed that strain F8825T was a novel species, different from C. lividus MSSRFBL1T, C. thiooxidans F43bT, and C. azotifigens A.slu09T. The physiological and biochemical properties of the strain, such as carbon source utilization, nitrate reduction, and ferrous ion oxidation, further supported that this is a novel species. Based on the polyphasic taxonomic results, strain F8825T was identified as a novel species in the genus Ciceribacter, for which the name Ciceribacter ferrooxidans sp. nov. is proposed. The type strain is F8825T (= CCTCC AB 2018196T = KCTC 62948T).