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양웅모,김향미,장문석,박완수,김원남,김산웅,최동기,이학철,김윤경,박성규,Yang, Woong-Mo,Kim, Hyang-Mi,Chang, Mun-Seog,Park, Wan-Su,Kim, Won-Nam,Kim, San-Woong,Choi, Dong-Gi,Lee, Hak-Chul,Kim, Yun-Kyung,Park, Seong-Kyu 대한한의학방제학회 2006 大韓韓醫學方劑學會誌 Vol.14 No.2
The natural plants were screened for new cosmetic materials. Many parts of Nelumbo nucifera has been investigated for anti-wrinkling and anti-furrowing effects. The leaves. flowers and stamens of Nelumbo nucifera were extracted with ethanol or distilled water. The effects of cell viability and $H_2O_2-induced$ cytotoxicity were studied on CCD-986sk human fibroblast cell line by MTT assay. We measured various effects related to skin such as the synthesis of MMP-1 and type I procollagen. The ethanol extract of Nelumbo nucifera leaves (ENL) at concentrations of 30 ${\mu}g/ml$ showed maximum cell viability on CCD-986sk cells by 129.70%. and $H_2O_2-induced$ cytotoxicity was blocked by ENL (5. 10. 20. 30. and 40 ${\mu}g/ml$) concentration-dependently. The synthesis of MMP-1 and type I procollagen were positive in ENL. These results suggest that ENL has anti-oxidant and anti-furrowing effects.