Genome-wide identification and comperative analysis of RuBisCo genes/proteins in Asteracea

Murat Kemal Avci,Erdem Tezcan,Emre Sevindik 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.4

Asteraceae, the largest known plant family in the world, contains economically important species including ornamental plants. The most abundant enzyme on Earth, Ribulose-1,5-bisphosphate (RuBisCo), catalyzes the first stepof carbon assimilation in photosynthesis. In this study, we conducted sequence, physicochemical, phylogenetic, and three-dimensional comparative analyses of 22 RuBisCo proteins in the Asteraceae using various bioinformatic tools. The alignment results showed that all residues corresponding to the region between 48-179 amino acids were conserved in all species, except for just seven residues: G79, I80, S88, F90, F93, I135, and S136. The sequence lengths of the RuBisCo large subunits were between 163 and 477 amino acids, with an average length of 255 amino acids. Their molecular weights (Mw) ranged from 18341.8 to 52895.1 Da, with an average Mw of 26294.5 Da. We found that the most abundant amino acid residue was Gly, which comprised 9.88% of these RuBisCo proteins. Ala (8.17%) and Leu (8.53%) were also both abundant. The extinction coefficient of the RuBisCo proteins at 280 nm ranged from 27515 to 69830 M-1 cm-1. The instability index values ranged from 24.41 to 40.65, and only one protein (from Gymnarrhena micrantha) was unstable in vitro. The aliphatic index values ranged from 72.56 to 81.13, while the GRAVY values ranged from -0.394 to -0.179. A total of ten motifs were identified in the sequences of the RuBisCo proteins. Phylogenetic analysis revealed that the 22 RuBisCo proteins formed two main clades. A RAMPAGEanalysis revealed that 95.0-98.4% of residues were located in the favored region in 22 RuBisCo proteins. Sequence alignment and 3D analysis revealed that a catalytically important Lys residue was conserved among all the RuBisCoenzymes of Asteraceae. The results of this study provide insights into the fundamental characteristics of the RuBisCo proteins of the Asteraceae.

Complete chloroplast genome sequence of Adenostemma madurense (Asteraceae)

김지은,김상철,장주은,길희영 국립중앙과학관 2023 Journal of Asia-Pacific Biodiversity Vol.16 No.4

Adenostemma madurense D.C. belongs to the family Asteraceae and is a wild annual herb found in Korea. In this study, we determined the phylogenetic position of A. madurense by identifying its complete chloroplast sequence. The result indicated a genome size of 150,054 bp, which was composed of a large single copy of 82,008 bp, two inverted repeats of 24,952 bp each, and a small singlecopy of 18,142 bp. Overall, 128 unique genes were identified, including 85 protein-coding, 35 tRNA, and 8 rRNA genes. A comparison of the A. madurense and A. lavenia chloroplast genomes revealed seven variations. Phylogenetic analysis revealed that A. madurense formed a clade with A. lavenia and had a well-supported phylogenetic relationship with Asteraceae. The first complete chloroplast genome of A. madurense was obtained, which would be helpful in explaining the process of speciation in Adenostemma and the development of molecular markers.

Parasenecio pentaphyllaria (Asteraceae: Senecioneae), a new species of Parasenecio from Korea

권민지,남기흠,조형준,김재영,정대희,정 선,문병철,정규영 한국식물분류학회 2023 식물 분류학회지 Vol.53 No.3

Parasenecio pentaphylla, a new species of the genus Parasenecio from Mt. Ilwol- san, Ilwol-myeon, Yeongyang-gun, Gyeongsangbuk-do, Korea, is described and illustrated here. In Korea, this species has been recognized as P. adenostyloides, known to be a Japanese endemic species. However, it is clearly distinguished from P. adenostyloides by its involucre shape, the number of phyllaries, the number of florets per capitulum, and the ratio of the tube length to the throat length. A molecular phylogenetic analysis using the Internal Transcribed Spacer region also clearly indicated that P. pentaphyllaria was genetically distinct from P. adenostyloides. Therefore, it is recognized as an independent species, different from P. adenostyloides. The spe- cific epithet “pentaphyllaria” was given based on its characteristic of having five phyllaries.

한국산 가막사리속(국화과)의 미기록 귀화식물: 왕도깨비바늘

김선유,홍석표,윤석민 한국식물분류학회 2012 식물 분류학회지 Vol.42 No.2

We report a newly naturalized species of the genus Bidens L. (Asteraceae) in Korea, specifically Bidens subalternans DC. var. subalternans. This naturalized plant was discovered Geumjung-gu in Busan; Gyeongsan-si, Jillyang-eup in Gyeongbuk-do; and in Buk-gu in Daegu. Bidens subalternans var. subalternans is distinguishable from other Bidens in Korea by the following combination of characteristics: an odd-pinnate leaf, deeply lobed, narrow lanceolate, four lobes in the corolla, an oblong achene shape. This taxon is termed ‘Wangdo-kka-bi-ba-neul’ as a common Korean name based on its large size (in particular, the size of leaf). The key for all taxa of the genus Bidens in Korea is also provided here. 부산광역시 금정구와 경상북도 경산시 진량읍 및 대구광역시 북구에서 채집된 한국산 국화과의 미기록 귀화식물인 Bidens subalternans DC. var. subalternans을 확인하여 보고한다. 본 분류군은 잎은 1회 우상복엽으로 깊게 갈라지며, 소엽은 너비가 좁은 피침형이고, 통상화는 4갈래, 수과는 장타원형으로 한국산 가막사리속 다른 분류군들과 구분이 된다. 국명은 식물체가 대형(특히, 잎 크기)임을 고려하여, ‘왕도깨비바늘’로 신칭하였다. 한국산 가막사리속 분류군에 대한 검색표를 제시하였다.

14종 국화과(Asteraceae) 식물 종자 추출물의 항산화 활성

김준혁,이다현,이미현,정영호,박초희,이희호,나채선,Kim, JunHyeok,Lee, Da Hyun,Lee, Mi Hyun,Jung, Young Ho,Park, Cho Hee,Lee, Hee Ho,Na, Chae Sun 한국생명과학회 2021 생명과학회지 Vol.31 No.6

국화과 식물은 현화식물 중 약 10%를 차지하며, 한반도에서 전통적으로 약용 식물로 사용되었다. 본 연구는 14종 국화과 식물 종자 추출물의 항산화 활성 평가를 통해 식물의 다양성 보전에 기여하기 위해 진행되었다. 충실률 90% 이상이고 발아율이 50% 이상인 종자를 대상으로 실험을 진행하였다. 총 페놀성 물질의 함량은 포천구절초(13.5 mg GAEs/g seeds), 구절초(11.8 mg GAEs/g seeds), 과꽃(11.0 mg GAEs/g seeds) 순으로 가장 높게 나타났다. 총 플라보노이드 함량은 과꽃(9.8 mg QEs/g seeds), 포천구절초(7.2 mg QEs/g seeds), 서양민들레(6.3 mg QEs/g seeds) 순으로 가장 높게 나타났다. DPPH 라디컬 소거활성은 종자 추출물의 종자 추출물의 IC₅₀ 값을 기준으로 포천구절초(57.4 ㎍/ml), 서양민들레(59.1 ㎍/ml), 구절초(65.0 ㎍/ml) 순으로 가장 높게 나타났다. ABTS 라디컬 소거활성은 종자 추출물의 IC₅₀ 값을 기준으로 과꽃(26.2 ㎍/ml), 포천구절초(38.4 ㎍/ml), 서양민들레(40.2 ㎍/ml) 순으로 가장 높게 나타났다. 항산화 활성을 기반으로 한 군집분석에 따라 14 종은 항산화 활성이 높은 group 4에서 항산화 활성이 낮은 group 0까지 5개의 group으로 분류되었다. 높은 페놀성 함량과 항산화 활성을 갖는 group 3와 4에 속하는 구절초, 포천구절초, 서양민들레, 과꽃 종자는 식물 유래 천연 항산화제로써 유력하다. Approximately 10% of all angiosperms belong to the Asteraceae family. Plant species belonging to this family have traditionally been used as medicinal plants in the Korean Peninsula. We investigated the antioxidant activity of seed extracts from 14 species belonging to the Asteraceae family. Seeds with ≥ 90% percentage of filled seed and ≥ 50% final germination were used. The total phenolic content was the highest in Dendranthema zawadskii var. tenuisectum (13.5 mg of gallic acid equivalents (GAEs)/g seeds), followed by Dendranthema zawadskii var. latilobum (11.8 mg of GAEs/g seeds), and Callistephus chinensis (11.0 mg of GAEs/g seeds). The total flavonoid content was highest in C. chinensis (9.8 mg of quercetin equivalents (QEs)/g seeds), followed by D. zawadskii var. tenuisectum (7.2 mg of QEs/g seeds) and Taraxacum officinale (6.3 mg of QEs/g seeds). Our results showed that 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity was highest in D. zawadskii var. tenuisectum (57.4 ㎍/ml), followed by T. officinale (59.1 ㎍/ml) and D. zawadskii var. latilobum (65.0 ㎍/ml), with a half maximal inhibitory concentration (IC₅₀) of DPPH scavenging activity. Furthermore, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity was highest in C. chinensis (26.2 ㎍/ml), followed by D. zawadskii var. tenuisectum (38.4 ㎍/ml), T. officinale (40.2 ㎍/ml), with a half maximal inhibitory concentration (IC₅₀) of ABTS scavenging activity. Based on a cluster analysis according to the antioxidant activity, the 14 species were classified into five groups, with group 4 having the highest antioxidant activity and group 0 having the lowest antioxidant activity. D. zawadskii var. latilobum, D. zawadskii var. tenuisectum, T. officinale, and C. chinensis belonging to groups 3 and 4, exhibited high phenolic content and antioxidant activity and can be considered potent plant-derived natural antioxidants.

Assembly and Comparative Analysis of Complete Mitogenome of Silybum marianum (L.) Gaertner

이정우(Jeongwoo Lee),예도몬(Yedomon Ange Bovys Zoclanclounon ),정화진(Hwajin Jung),이태호(Taeho Lee),김정구(Jeonggu Kim),박규황(Guhwang Park),이근표(Keunpyo Lee),안광훈(Kwanghoon An),심지형(Jeehyoung Shim),진중현(Joonghyoun Chin) 한국육종학회 2022 한국육종학회지 Vol.54 No.4

Silybum marianum (L.) Gaertner. (milk thistle), is a member of the Asteraceae family. Silymarin has hepatoprotective effects, accumulates at high levels in the external cover of milk thistle seeds, and is composed of flavonolignan isomers. In the present study, we assembled and annotated the mitogenome of Silybum marianum. This mitogenome was found to have a length of 407,123 base pairs and an overall base composition: A, 27.41%; T, 27.33%; G, 22.72%; and C, 22.54%. Seventy-four unique genes were identified in the Silybum marianum mitogenome based on annotation results, including 27 protein-coding genes, 44 tRNA genes, and 3 rRNA genes. Common protein-coding genes of 11 Asteraceae family references and four outgroup (Campanulaceae and Solanaceae) mitogenomes were used to construct a phylogenetic tree. The phylogenetic tree of the Silybum marianum mitogenome revealed close relationships with three reference mitogenomes (Arctium tomentosum, Arctium lappa, and Saussurea costus), and the flower morphology of Silybum marianum was similar to that of the three reference mitogenomes. This report describes unique features of the Silybum marianum mitogenome relative to the three related reference mitogenomes. In addition, we could envisage a specific analysis of the phylogenetic relationship of Silybum mariaum using additional Asteraceae family mitogenomes.

세계 각지에서 수집된 산형과, 국화과 식물의 종자 발아 특성

김용호 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

This study was carried out to recognize the germination patterns of world-widely collections in Apiaceae and Asteraceae seeds. The sixty five and two hundred eighty two accessions of Apiaceae and Asteraceae, respectively, which were collected from 16 countries in 2002, were examined at Soonchunhyang University in korea. Total 254 accessions were germinated in 347 materials tested. In case Asteraceae, mean value of germination rate was 76.95% whereas Apiaceae showed 56.92%. The highest germination rate was investigated in standard germination analysis method at room temperature, which was 42.13%, among some different germinating method, Dark and light treatment showed 28.35% and 16.14%, respectively. Also 14.17% of accessions were needed a low temperature condition for seed germination and a few accessions were germinated through a chemical treatment(KNO_(3), 1% solution).

국내외 문헌에 나타난 식용꽃 분석

박주경(Jukyung Park),장유진(Eujean Jang),박천호(Chunho Pak) 인간식물환경학회 2015 인간식물환경학회지 Vol.18 No.6

This study examined the current status of the types of edible flowers used domestically and overseas and the discovery and utilization methods of edible flowers naturally growing in Korea, analyzed the types of edible flowers that have been reported until present in order to seek a development method to strengthen competitiveness in the edible flower industry. Domestic and overseas records contain records of 406 species in 80 families of edible flowers. There were 38 species in 18 families of edible flowers appearing in at least 50% of the records, and per family, the Asteraceae, and per type, the Viola spp. type appeared most frequently. A total of 127 species in 36 families appeared in overseas records. There were 27 species in 15 families of edible flowers appearing in at least 60% of the records, and per family, the Asteraceae, and per type, Borago officinalis, Dianthus spp., Lavandula spp., Tropaeolum majus sp. and Viola spp. appeared most frequently. A total of 381 species in 78 families appeared in domestic records. There were 62 species in 26 families of edible flowers appearing in at least 60% of the records, and per family, the Asteraceae and Lamiaceae appeared most frequently, while per type, Dendranthema spp., Hemerocallis fulva, Rosa spp., Taraxacum platycarpum Dahlst., and Viola spp. appeared most frequently. Thus, in order for the edible flower industry to achieve development in the future, there would be a need to make efforts to expand consumption of edible flowers, such as through discovery of naturally growing flowers, research on production and storage technology, promotional activities and research on its functionality, and development of various processed goods through mass industrialization.

한국산 금방망이속(Senecio L.)과 근연분류군(국화과)의 체세포 염색체수

장진,정규영 한국식물분류학회 2011 식물 분류학회지 Vol.41 No.2

The somatic chromosome numbers of the Korean species of Senecio L. and two related genera are investigated here. Three different chromosome numbers were found: 2n = 40 in Senecio argunensis Turcz., S. nemorensis L., S. vulgaris L., Tephroseris flammea (DC.) Holub and 2n = 44 in T. phaeantha (Nakai) C. Jeffrey & Y.L. Chen. and 2n = 48 in Sinosenecio koreanus (Kom.) B. Nord., T. kirilowii (Turcz. ex DC.) Holub and T. pierotii (Miq.) Holub. The chromosome numbers of Sinosenecio koreanus (Kom.) B. Nord., Tephroseris flammea (DC.) Holub and T. phaeantha (Nakai) C. Jeffrey & Y.L. Chen. were different from those in previous reports. 한국산 금방망이속(Senecio L.) 및 근연속인 국화방망이속(Sinosenecio B. Nord.), 솜방망이속(Tephroseris (Rchb.) Rchb.)에 속하는 8분류군을 대상으로 체세포 염색체수를 조사하였다. 체세포 염색체수는 2n = 40, 2n = 44인 종류와 2n = 48인 종류로 구분되었으며, 2n = 40인 분류군은 쑥방망이(Senecio argunensis Turcz.), 금방망이(S. nemorensis L.), 개쑥갓(S. vulgaris L.), 산솜방망이(Tephroseris flammea (DC.) Holub)이었다. 2n = 44인 분류군은 바위솜나물(T. phaeantha (Nakai) C. Jeffrey & Y.L. Chen)이었으며, 2n = 48인 분류군은 국화방망이(Sinosenecio koreanus (Kom.) B. Nord.), 솜방망이(T. kirilowii (Turcz. ex DC.) Holub), 물솜방망이(T. pierotii (Miq.) Holub.)로서, 이중 국화방망이와 산솜방망이, 바위솜나물은 기존의 연구 결과와 차이를 보였다.

Assessment of the Growth and Ornamental Quality of Senecio haworthii (Sweet) Sch.Bip. Grown under Different Day/Night Temperatures

이재환,남상용 강원대학교 농업생명과학연구원 2023 강원 농업생명환경연구 Vol.35 No.3

Senecio haworthii is a succulent plant belonging to the Asteraceae family. Optimal growth conditions for this species have not been well-established. In this study, we investigated the optimal day/night temperature range for enhancing the growth and ornamental quality of S. haworthii. Four day/night temperature treatments were designed as follows: 20/15, 24/19, 28/23, and 32/27°C. Based on the plant sizes and biomass analysis, the best growth performance was determined to be achieved under a relatively low-temperature treatment of 20/15°C. Conversely, the 32/27°C treatment resulted in the lowest growth rates, likely attributed to decreased leaf function caused by prolonged exposure to high-temperature conditions. Additionally, leaf color analysis using the CIELAB color space revealed that CIELAB L*, representing lightness, and CIELAB a*, indicating green-red opponent colors, proportionally increased with the rise in day/night temperature levels. Previous studies have reported an inverse relationship between L* and plant growth parameters. Consistent with previous research, the treatment at 32/27°C, which exhibited the lowest growth, showed higher L* values. Conversely, the CIELAB b* value, representing blue-yellow opponent colors, was highest under the 20/15°C treatment, suggesting leaf-yellowing tendencies in relatively lower temperature conditions. Based on the color difference analysis using CIE76 color difference (ΔE*ab), temperatures of 20/15 and 24/19°C were used as reference points; under these conditions, the temperatures of 28/23 and 32/27°C were evaluated as having a ‘small color difference’. Therefore, even with significant differences in day/night temperatures, no noticeable differences were concluded to be found in leaf color. Based on these findings, cultivating S. haworthii in relatively low-temperature conditions of around 20/15°C is concluded to be advantageous for enhancing both plant growth and ornamental quality compared to higher temperature conditions such as 32/27°C.