Antioxidant and Biological Activity in the Leaves of Adzuki Bean (Vigna angularis L.)

Kyung Jun Lee,Jung-Ro Lee,Myoung-Jae Shin,Gyu-Taek Cho,Ho-Sun Lee,Kyung-Ho Ma,Gi-An Lee,Jong-Wook Chung 한국자원식물학회 2018 한국자원식물학회지 Vol.31 No.3

The adzuki bean (Vigna angularis L.) is a red-grained legume that has a number of essential nutrients and is used in traditional dishes in Asia. Adzuki bean industrial by-products are also a potential low-cost source of some unique bioactive polyphenols. Hence, here, the authors aimed to perform a comparative study of the phytochemical profiles of the leaves and seeds of the adzuki bean and compare their antioxidant, α-glucosidase inhibition, and tyrosinase inhibition activity. The authors assessed antioxidant activity by DPPH, ABTS, FRAP, PR, TPC, and SOD assays, which showed wide variation, respectively. From the relative antioxidant capacity index results, 10 adzuki bean landraces were selected to compare for phytochemicals and bioactivity using leaf and seed extracts. Antioxidant, α-glucosidase inhibition, and tyrosinase inhibition activity in the leaf extracts were higher than in the seed extracts, and there were more flavonols and isoflavones in the leaf extracts than in the seed extracts. This study demonstrated that adzuki bean leaf extracts could be a new natural antioxidant or antidiabetic agent and a skin whitener and can also be used in industrial applications.

더덕과 일부 한약재 열수추출물의 혼합 비율에 따른 생리활성

오혜숙,김준호 한국지역사회생활과학회 2007 한국지역사회생활과학회지 Vol.18 No.3

The aim of this study was to investigate the physiological functionalities of Codonopsis lanceolata, Glycyrrhiza uralensis, Chaenomeles sinensis, Crataegus pinnafida, and their mixtures. We also determined their antioxidative, fibrinolytic, and αglucosidase inhibitory activities. The antioxidative activities of Codonopsis lanceolata, Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida were 79%, 88.3%, 89.9%, and 89.3% respectively. Their fibrinolytic activities were 0.80plasmin unit/ml, 0.57 plasmin unit/ml, 0.52 plasmin unit/ml, and 0.53 plasmin unit/ml respectively. The αglucosidase inhibitory activity of Codonopsis lanceolata was 25%. The 10fold diluents of Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida showed αglucosidase inhibitory activities of 93.6%, 65.3%, and 61.3% respectively. In antioxidative activity tests of the medicinal plants mixtures at various ratios, the mixtures of Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida with Codonopsis lanceolata showed antioxidative activities of approximately 90%. In fibrinolytic activity tests mixtures(1:1) of Codonopsis lanceolata with Chaenomeles sinensis and Crataegus pinnafida exhibited increases of 23% and 24% in activity respectively. In αglucosidase inhibitory activity tests, a mixture (4:1) of Codonopsis lanceolata and 10fold diluted Glycyrrhiza uralensis showed an inhibitory activity of 98%, a mixture (3:1) of Codonopsis lanceolata and 10fold diluted Chaenomeles sinensis showed an inhibitory activity of 69.6%, and a mixture (1:1) of Codonopsis lanceolata and 10fold diluted Crataegus pinnafida showed an inhibitory activity of 50.2%. In conclusion, the mixtures of Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida with Codonopsis lanceolata will be used as a material for the development of biofunctional foods.

Antioxidant Activity of Talinum portulacifolium (Forssk) Leaf Extracts

Thalapaneni Nageswara Rao,Chidambaram Kumarappan,Sabapathi Mohana Lakshmi,Subhash C Mandal 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.4

The aim of the study is to investigate the antioxidant activity through, reducing power, 2, 2- diphenyl-1-picryl hydrazyl radical (DPPH), nitric oxide radical (NO), superoxide radical, hydrogen peroxide radical (H2O2) scavenging activity and the amount of total phenolic compounds of chloroform, ethyl acetate, methanol and aqueous extracts of the leaves of Talinum portulacifolium. Chloroform extract of leaves of T. portulacifolium showed highest antioxidant activity, with a direct relationship between activity and concentration of extracts (15 - 240 μg/mL). Among all the extracts, the highest amount of the total polyphenolic compounds was found in the chloroform extract. Chloroform extract of T. portulacifolium showed an important free radical scavenging activity towards the DPPH, NO, Superoxide and H2O2 radicals, with IC50 values of 133.26, 165.75, 156.34 and 135.29 μg/mL, respectively. In the lipid peroxidation assay, extracts of chloroform and ethyl acetate showed a remarkable inhibitory activity. The extracts showed significant activity in all the experiments but lower than the standard antioxidant, ascorbic acid. The aim of the study is to investigate the antioxidant activity through, reducing power, 2, 2- diphenyl-1-picryl hydrazyl radical (DPPH), nitric oxide radical (NO), superoxide radical, hydrogen peroxide radical (H2O2) scavenging activity and the amount of total phenolic compounds of chloroform, ethyl acetate, methanol and aqueous extracts of the leaves of Talinum portulacifolium. Chloroform extract of leaves of T. portulacifolium showed highest antioxidant activity, with a direct relationship between activity and concentration of extracts (15 - 240 μg/mL). Among all the extracts, the highest amount of the total polyphenolic compounds was found in the chloroform extract. Chloroform extract of T. portulacifolium showed an important free radical scavenging activity towards the DPPH, NO, Superoxide and H2O2 radicals, with IC50 values of 133.26, 165.75, 156.34 and 135.29 μg/mL, respectively. In the lipid peroxidation assay, extracts of chloroform and ethyl acetate showed a remarkable inhibitory activity. The extracts showed significant activity in all the experiments but lower than the standard antioxidant, ascorbic acid.

Physiological Effects of Hot Water Extract and Solvent Fractions of Carthamus tinctorius L.

Jun-Ho Kim(김준호) 대한의생명과학회 2011 Biomedical Science Letters Vol.17 No.2

In this study, we investigated the biological activities of Carthamus tinctorius including antioxidative, fibrinolytic, thrombin inhibitory, α-glucosidase inhibitory and collagenase inhibitory activities. Carthamus tinctorius, hot water extract was fractionated into hexane, chloroform, ethyl acetate, butanol and water fraction. Each of these was assayed individually. The hot water extract showed high antioxidative activity and thrombin inhibitory activity at 90.17% and 97.10% respectively. In the fraction activity tests, chloroform fraction showed the highest antioxidative activity at 81.85%. The fibrinolytic activity was strong only in the butanol fraction at 0.70 plasmin units/ml. The thrombin inhibitory activities of hexane, ethyl acetate and butanol fractions were 97.35%, 86.74% and 93.18% respectively. In collagenase inhibitory activity test, hexane fraction showed the highest activity at 87.78%. In conclusion, the hot water extract and solvent fractions of Carthamus tinctorius L can be used as a material for the development of biofunctional tea and foods respectively.

Change of Fibrinolytic and Antioxidative Activities of Codonopsis lanceolata According to Various Storage Conditions, and Heat or Salt Treatments

김준호,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

We investigated the changes of fibrinolytic and antioxidative activities of wild or cultivated Codonopsis lanceolata, which were packed in woven polypropylene (WP) film or low density polyethylene (LDPE) film and stored for 0, 15, and 30 days at refrigerated (2~4℃) or room (18~20℃) temperature (So we have 16 samples, and wrote them as the abbreviated words such as W-WP-RE-15, W-WP-RO-15, W-LDPE-RE-15, W-LDPE-RO-15, W-WP-RE-30, W-WP-RO-30, W-LDPE-RE-30, W-LDPE-RO-30, C-WP-RE-15, C-WP-RO-15, C-LDPE-RE-15, C-LDPE-RO-15, C-WP-RE-30, C-WP-RO-30, C-LDPE-RE-30, C-LDPE-RO-30). Fibrinolytic activity of fresh Codonopsis lanceolata cultivated in the mountain or field were 0.8 unit (plasmin unit/ml) or 7.3 units, respectively. In descending order, the activities of wild Codonopsis lanceolata stored for 15 days were as followed ; LDPE-RE (0.70 unit), WP-RO (0.52 unit), WP-RE (0.45 unit), and LDPE-RO (0.30 unit). After 30 days, fibrinolytic activities of them decreased to 0.47 unit (LDPE-RE), 0.28 unit (WP-RO), 0.21 unit (WP-RE), and 0.30 unit (LDPE-RO). Considering from the point of fibrinolytic activity, the optimal storage condition of wild Codonopsis lanceolata was packing with LDPE film and storing at 4℃. The change of fibrinolytic activities of Codonopsis lanceolata cultivated in the field revealed the similar trend as wild samples, but the fibrinolytic activities of 30 days-stored samples were maintained better than the wild Codonopsis lanceolata. Fibinolytic activity of wild Codonopsis lanceolata was increased by heating for 5 min at 100℃ and decreased by addition of NaCl. Antioxidative activities of Codonopsis lanceolata were also compared from the electron donating activity. Fresh Codonopsis lanceolata had about 70% of electron donating activity. Independent of cultivation area, electron donating activity dropped to 19~74% (wild Codonopsis lanceolata) and 27~59% (cultivated Codonopsis lanceolata) during 15 days storage. But after 30 days storage, we obtained the unexpected results, which meant that the activities were higher activities than 15 days-stored samples or even though the fresh samples. In general, Codonopsis lanceolata could maintain antioxidative activities most strongly with LDPE film and chilled condition.

단삼분획물의 항산화 및 항염 활성

이주희,안병용 동아시아식생활학회 2023 동아시아식생활학회지 Vol.33 No.3

In this study, the antioxidant activities of solvent fractions obtained from the hot water extract of Salvia miltiorrhiza were investigated according to the polarity. The studies of DPPH radical scavenging activity, ABTS radical scavenging activity, superoxide anion radical scavenging activity, hydrogen peroxide scavenging activity, hydroxyl radical scavenging activity, nitric oxide radical scavenging activity, and reducing power confirmed the highest antioxidant activity in the n-butanol (n-BuOH) fraction. The highest Fe2+ ​​chelating activity was obtained in the ethyl acetate (EtOAc) fraction, which was converse to the radical scavenging activity. Solvent fractions of Salvia miltiorrhiza hot water extract with effective antioxidant activity and the n-BuOH fraction which showed the highest antioxidant activity were applied to an in vitro experiment to confirm the effect of regulating inflammation. In addition, the highest total polyphenol content was also obtained in the n-BuOH fraction (111.97±0.41 mg GAE/). The antioxidant and anti-inflammatory activities of the n-BuOH fraction were determined to be associated with the total polyphenol content. Antioxidant activity is effective in removing harmful free radicals. Considering the results of this study, we conclude that Salvia miltiorrhiza has the potential to be applied as a functional food material for eliminating reactive oxygen species. .

다류의 유형별 항산화 성분함량 및 항산화 활성 연구

김명길,오문석,전종섭,김한택,윤미혜 한국식품위생안전성학회 2016 한국식품위생안전성학회지 Vol.31 No.2

The purpose of this study was to investigate the contents of antioxidant compounds and antioxidant activities in teas. A total of 99 teas were tested for their antioxidant activities based on their ability to scavenge DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical. Antioxidant activity was expressed as mg of ascorbic acid equivalents per 100 g tea sample (L-ascorbic acid equivalent antioxidant capacity, AEAC) and was expressed as mg per 1 serving size(free radical scavenging activity, FSC50). The total polyphenol contents of the extracts was quantified using the Folin-Ciocalteu method and the total flavonoid contents of the extracts was determined using a modified method of Davis. Vitamin C was analyzed by HPLC method. According to the AEAC value, mate tea, green tea, black tea, oriental raisin tea, chamomile tea and burdock tea showed relatively high antioxidant activities. Polyphenolic compounds were the major naturally occurring antioxidant compounds found in teas and the high concentrations of total polyphenol compounds were observed in black tea, green tea and mate tea. The high concentrations of total flavonoid compounds were observed in mate tea, black tea and yam tea and the amount of vitamin C contents were found to be high in citron tea and green tea. As a result, steeping tea was much higher in antioxidant activity than either powdered tea or liquefied tea. According to the study, a high correlation was demonstrated between the total polyphenol contents and antioxidant activities in teas(r=0.846) and correlations between the total flavonoid contents and antioxidant activities was statistically significant in teas(r=0.625). It was found that also the proportional relationship established among the total polyphenol content and antioxidant activities. That is, antioxidant activity of teas has been confirmed to have been caused by the total polyphenol.

정금나무의 기능성 생리활성 물질과 생리활성

채정우,조희선 한국생명과학회 2022 생명과학회지 Vol.32 No.2

In modern society, interest in antioxidants is increasing as the stress caused by oxidants increases. However, the demand for synthetic antioxidants is decreasing because some studies have confirmed that they are harmful when consumed in large quantities; thus, studies on antioxidants derived from natural substances are actively being conducted to replace synthetic antioxidants. Blueberry, known as one of the world's top ten long-lived foods, is a plant of the Vaccinium (Ericaceae) family, and various pharmacological activities of blueberry including antioxidant activity have been studied. Vaccinium oldhamii (VO) is a deciduous broad-leaved shrub in the same genus as blueberries, and in this paper, we summarize the studies on the efficacy analysis of VO extracts and purified products. The content of phenolic compounds in VO fruits was proportional to antioxidant and anti-influenza activity such as the inhibition of NO production, and the total content of polyphenols and anthocyanin was higher than that in blueberries. VO fruit extracts showed anti-inflammatory activity and anti-cancer activity against human acute leukemia; in contrast, VO branch extracts showed anti-inflammatory activity, activity to inhibit osteoclast differentiation and bone resorption due to inflammatory response, and anti-cancer activity against several human cancer cell lines. Compared to blueberries, VO showed higher phenolic compound content, antioxidant activity, and various physiological activities. In addition, VO is considered to have sufficient value as an alternative crop to blueberries, such as it can be grown natively in Korea, with simple mass cultivation and no need to pay royalties for commercialization. 현대사회에서 산화물질에 의한 스트레스가 증가함에 따라 항산화제에 대한 관심이 증가하고 있다. 그러나 합성 항산화제는 다량 섭취 시 유해하다는 연구 등에 기인하여 수요가 감소하고 있으며, 합성 항산화제를 대체하기 위하여 천연물에서 유래된 항산화 물질에 관한 연구가 활발히 진행되고 있다. 세계 10대 장수 식품의 하나로 알려진 블루베리는 진달래과(Ericaceae) 산앵도나무속(Vaccinium)의 식물로 항산화를 비롯한 다양한 약리활성에 대한 연구가 수행되었다. 정금나무(Vaccinium oldhamii)는 블루베리와 같은 속에 속한 낙엽활엽관목으로 본 논문에서는 정금나무의 추출물과 정제물의 효능 분석을 실시한 연구들을 정리하였다. 정금나무 열매의 페놀류 화합물의 함량은 항산화 활성 및 항인플루엔자 활성과 비례하였으며, 총 폴리페놀 및 안토시아닌의 함량은 블루베리에 비해 높았다. 정금나무 열매 추출물은 항염증 활성을 나타내었으며 정금나무 가지 추출물은 항염증 활성과 염증반응에 의한 파골세포의 분화 및 골흡수를 억제하는 활성을 나타내었다. 정금나무 열매 추출물은 인간 급성 백혈병에 대한 항암 활성을, 정금나무 가지 추출물은 여러 인간 암세포주에서의 항암활성을 나타내었다. 정금나무는 블루베리에 비하여 페놀성 화합물 함량과 항산화 활성이 높고 다양한 생리활성을 나타내며, 국내에 자생하여 대량 재배를 위한 연구가 용이하고, 상업화 시 해외 사용료를 부담할 필요가 없는 등 블루베리의 대체작물로서의 가치를 충분히 가지고 있다고 판단된다.

식물유래 천연색소의 항산화 활성

부희옥,황성진,배춘식,박수현,송원섭 한국자원식물학회 2011 한국자원식물학회지 Vol.24 No.1

The objective of this study was to determine the effect of antioxidant enzyme activity and radical scavenging activities of thirteen kinds of natural plant pigments. The analytic method of antioxidant activities were measured by estimating DPPH free radical scavenging and nitrite scavenging ability activity. The free radical scavenging activity by method using stable free radical DPPH was the highest in the red cabbaged pigment. Addition of ethanol extract 1㎎/㎖ from onion peel pigment displayed remarkable effect on nitrite scavenging ability about 91.9%. Antioxidative enzyme activity was evaluated in terms of superoxide dismutase(SOD), catalase(CAT), and ascorbate peroxidase(APX) activity. The bitter melon pigment had the highest SOD activity of 87.3%. The activities of CAT and APX were higher in the mulberry leave pigment compared with other natural plant pigments. In contrast, CAT activity of plant pigment samples were unaffected. These results suggest that natural plant pigment had the potent biological activities such as antioxidant enzyme activities, and that their activities exhibited differently depending on each kind of pigments. 선발된 13종의 천연색소 추출물에 대하여 free radical 소거활성 및 아질산염 소거활성, 그리고 항산화효소 활성에 대하여 조사하였다. 천연색소 종류별 free radical 소거활성을 보면 적양배추(red cabbage), 양파껍질(onion peel), 레드비트(redbeet), 흑미(black rice), 오디(mulberry) 색소 등에서 상대적으로 높게 나타났고, 청치자(blue gardenia) 및 황치자(yellow gardenia), 완숙여주(mature bitter melon) 색소 등에서는 비교적 낮은 활성을 보였다 또한 아질산염에 대한 소거능을 조사한 결과, 반응용액 pH 1.2의 경우 양파껍질, 레드비트, 치자황, 지황(chinese foxglove), 치자청 색소에서 높은 소거 활성을 나타냈으며, 적양배추와 오디 색소에서는 상대적으로 낮은 소거활성을 보였다. 그리고 반응용액 pH 6.0에서는 대부분의 색소 추출물에서 아질산염 소거활성을 나타내지 않았다. 그리고 항산화효소 활성 결과를 보면, SOD 활성의 경우 여주, 흑미, 포도과피 등에서 높은 활성을 나타냈고, 자색고구마, 지황에서도 비교적 높은 활성을 보였다. 또한 APX 활성은 뽕잎, 오디, 지황의 순으로 높은 활성을 보였으며, CAT 활성도 APX 결과와 비슷한 양상으로 나타났다. 이는 천연색소에 있어 항산화 효소의 종류에 따라 그 활성에 차이가 있음을 알 수 있다. 본 연구결과를 통해 다양한 생리활성을 지니고 있는 천연식물색소에 대해 높은 항산화 기능성을 확인함으로서 향후 고부가가치의 향장소재 및 식품소재로서의 개발가능성이 매우 높음을 시사해 주고 있다.

천연화장품의 재료로서 복숭아 잎 추출물의 기능성 평가

장혜륜,권혜진,박두현 한국피부과학연구원 2014 대한피부미용학회지 Vol.12 No.2

Goal of this study is to evaluate the suitability of peach leaf as a raw material for natural cosmetics basedon specific functions that are antioxidative activity, antibacterial activity, and tyrosinase inhibition. Theantioxidative activity was determined based on DPPH-scavenging effect and the antibacterial activity wasmeasured based on inhibition effect of bacterial community isolated from face skin of 4 women. The peachleaf was grinded using ball meal for improving extraction efficiency. Triply distilled (highly pure) water or99.9% ethanol was used as a solvent for extraction of functional compounds from ground peach leaf. Dryweight of the extract was not quantitatively determined but functional efficiency of the extract was evaluatedbased on ratio of the peach leaf weight to the solvent volume. The ratio of peach leaf weight to solventvolume was adjusted to 20 g to 1,000 mL, which was employed as a criterion to determine functional activityof the extract. Ten times-diluted peach leaf extract was employed to determine the DPPH-scavengingactivity and the tyrosinase inhibition activity; however, the peach leaf extract was not diluted to determineantibacterial activity. Functional activity was the mean value of results obtained from triply repeated tests. The DPPH-scavenging activity of the water-extracted and the ethanol-extracted peach leaf was approximate57.5% and 68.0%, respectively; meanwhile, the tyrosinase inhibition activity of the water-extracted and theethanol-extracted peach leaf was approximate 52.5% and 41.9%, respectively. Differences between thewater-extracted and the ethanol-extracted peach leaf for antioxidative, tyrosinase inhibition, and antibacterialactivity was approximate 15%, 20%, and 10%, respectively. Conclusively, the functional activity contained inthe peach leaf is considered to be suitable as a raw material for the natural cosmetics.